Restriction map for
pFN21AE0702
(Circular) (Six-base) MAPSORT of: pFN21AE0702.seq Check: 5174 from: 1 to: 5923
pFN21AE0702 5923 bp
With 182 enzymes: SgfI *
October 20, 2009 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2812 2812
Size: 5923
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2091 3733 278
Size: 53 83 186 936 555 1642 2468
Fragments arranged by size:
2468 1642 936 555 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2646 3266 1937
Size: 709 620 4594
Fragments arranged by size:
4594 709 620
Acc65I G'GTAC_C
Cuts at: 3244 3244
Size: 5923
AclI AA'CG_TT
Cuts at: 1563 4050 4423 1563
Size: 2487 373 3063
Fragments arranged by size:
3063 2487 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2486 3186 3986 5370 5638 1632
Size: 854 700 800 1384 268 1917
Fragments arranged by size:
1917 1384 854 800 700 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5726
AflIII A'CryG_T
Cuts at: 1203 4822 5799 1203
Size: 3619 977 1327
Fragments arranged by size:
3619 1327 977
AgeI A'CCGG_T
Cuts at: 4754 4754
Size: 5923
AhdI GACnn_n'nnGTC
Cuts at: 4652 4652
Size: 5923
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5923
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5923
AlwNI CAG_nnn'CTG
Cuts at: 2722 3666 5238 2722
Size: 944 1572 3407
Fragments arranged by size:
3407 1572 944
ApaI G_GGCC'C
Cuts at: 3164 3164
Size: 5923
ApaLI G'TGCA_C
Cuts at: 3980 5136 3980
Size: 1156 4767
ApoI r'AATT_y
Cuts at: 1493 3232 3458 3610 5719 1493
Size: 1739 226 152 2109 1697
Fragments arranged by size:
2109 1739 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4477 160
Size: 872 3445 1606
Fragments arranged by size:
3445 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5923
AvaI C'yCGr_G
Cuts at: 1870 1943 2471 2768 3248 1870
Size: 73 528 297 480 4545
Fragments arranged by size:
4545 528 480 297 73
BamHI G'GATC_C
Cuts at: 2827 3253 3620 2827
Size: 426 367 5130
Fragments arranged by size:
5130 426 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2706 3045 3244 4699 618
Size: 325 217 627 919 339 199 1455 1842
Fragments arranged by size:
1842 1455 919 627 339 325 217 199
BanII G_rGCy'C
Cuts at: 1391 2111 3164 3242 1391
Size: 720 1053 78 4072
Fragments arranged by size:
4072 1053 720 78
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2292 928
Size: 971 393 4559
Fragments arranged by size:
4559 971 393
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2626 2660 3044 3078 4114 4148 1903
Size: 34 689 34 384 34 1036 34 3678
Fragments arranged by size:
3678 1036 689 384 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3815 5025 3815
Size: 1210 4713
BclI T'GATC_A
Cuts at: 1548 2736 1548
Size: 1188 4735
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3142 4534 136
Size: 107 122 71 631 605 1470 1392 1525
Fragments arranged by size:
1525 1470 1392 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2461 5918 2461
Size: 3457 2466
BlpI GC'TnA_GC
Cuts at: 2181 2850 3340 2181
Size: 669 490 4764
Fragments arranged by size:
4764 669 490
Bme1580I G_kGCm'C
Cuts at: 1790 3084 3164 3984 5140 1790
Size: 1294 80 820 1156 2573
Fragments arranged by size:
2573 1294 1156 820 80
BmgBI CAC'GTC
Cuts at: 1318 2017 1318
Size: 699 5224
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2053 3094 3654
Size: 416 53 83 399 24 636 1041 560
Cuts at: 3654 4612 442
Size: 958 1753
Fragments arranged by size:
1753 1041 958 636 560 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5923
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3196 3196
Size: 5923
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2225 2794 4583 1239
Size: 986 569 1789 2579
Fragments arranged by size:
2579 1789 986 569
Bpu10I CC'TnA_GC
Cuts at: 2196 2972 2196
Size: 776 5147
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4048 4913 5211 4048
Size: 865 298 4760
Fragments arranged by size:
4760 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2415 4586 882
Size: 617 916 2171 2219
Fragments arranged by size:
2219 2171 916 617
BsaAI yAC'GTr
Cuts at: 493 1204 3714 493
Size: 711 2510 2702
Fragments arranged by size:
2702 2510 711
BsaBI GATnn'nnATC
Cuts at: 5917 5917
Size: 5923
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2088 3730 4112 4747
Size: 53 83 186 936 555 1642 382 635
Cuts at: 4747 275
Size: 1451
Fragments arranged by size:
1642 1451 936 635 555 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4356 4754 5028 5175 5586 4356
Size: 398 274 147 411 4693
Fragments arranged by size:
4693 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2735 2765 2792 2822 1178
Size: 30 165 30 1332 30 27 30 4279
Fragments arranged by size:
4279 1332 165 30 30 30 30 27
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2115 2222 3185 1186
Size: 929 107 963 3924
Fragments arranged by size:
3924 963 929 107
BseYI C'CCAG_C
Cuts at: 3217 5126 5622 3217
Size: 1909 496 3518
Fragments arranged by size:
3518 1909 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5350
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3373 4134 5162 1540
Size: 275 186 1372 761 1028 2301
Fragments arranged by size:
2301 1372 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2111 3984 4069 5140 2111
Size: 1873 85 1071 2894
Fragments arranged by size:
2894 1873 1071 85
BsmI GAATG_Cn'
Cuts at: 3010 3434 3527 3010
Size: 424 93 5406
Fragments arranged by size:
5406 424 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2111 3084 3164 3242 3984 4069 5140
Size: 399 321 973 80 78 742 85 1071
Cuts at: 5140 1391
Size: 2174
Fragments arranged by size:
2174 1071 973 742 399 321 85 80
78
BspEI T'CCGG_A
Cuts at: 5586 5586
Size: 5923
BspHI T'CATG_A
Cuts at: 3812 5542 3812
Size: 1730 4193
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2812 3278 844
Size: 1968 466 3489
Fragments arranged by size:
3489 1968 466
BsrBI CCG'CTC
Cuts at: 1394 1823 2185 3810 1394
Size: 429 362 1625 3507
Fragments arranged by size:
3507 1625 429 362
BsrDI GCAATG_nn'
Cuts at: 65 1248 4418 4592 65
Size: 1183 3170 174 1396
Fragments arranged by size:
3170 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2258 2709 3132 4567 4754 1700
Size: 110 448 451 423 1435 187 2869
Fragments arranged by size:
2869 1435 451 448 423 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5923
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5923
BssSI C'ACGA_G
Cuts at: 2147 3983 4995 2147
Size: 1836 1012 3075
Fragments arranged by size:
3075 1836 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5923
BstBI TT'CG_AA
Cuts at: 4727 4727
Size: 5923
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5923
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2703 2011
Size: 692 5231
BstYI r'GATC_y
Cuts at: 1972 2461 2502 2595 2718 2827 3253 3620 4005
Size: 489 41 93 123 109 426 367 385
Cuts at: 4005 4022 5474 5560 5572 5918 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 489 426 385 367 346 123
109 93 86 41 17 12
Bsu36I CC'TnA_GG
Cuts at: 2082 2832 2082
Size: 750 5173
BtgI C'CryG_G
Cuts at: 513 1065 2610 513
Size: 552 1545 3826
Fragments arranged by size:
3826 1545 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5184
Fragments arranged by size:
5184 683 56
BtsI GCAGTG_nn'
Cuts at: 2430 2943 3439 4233 4253 2430
Size: 513 496 794 20 4100
Fragments arranged by size:
4100 794 513 496 20
ClaI AT'CG_AT
Cuts at: 1554 2286 1554
Size: 732 5191
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5888
DraI TTT'AAA
Cuts at: 3227 3227
Size: 5923
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4930 809
Size: 507 3614 1802
Fragments arranged by size:
3614 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2143 2260 2990
Size: 54 1425 50 275 18 313 117 730
Cuts at: 2990 3370 4259 5888 8
Size: 380 889 1629 43
Fragments arranged by size:
1629 1425 889 730 380 313 275 117
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3370 1537
Size: 275 1558 4090
Fragments arranged by size:
4090 1558 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3162 3853 1353
Size: 1809 691 3423
Fragments arranged by size:
3423 1809 691
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2108 4510 4884 5030 387
Size: 1064 138 519 2402 374 146 1280
Fragments arranged by size:
2402 1280 1064 519 374 146 138
EcoICRI GAG'CTC
Cuts at: 2109 2109
Size: 5923
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2225 2486 2794 3186 3986 4583 5370
Size: 393 593 261 308 392 800 597 787
Cuts at: 5370 5638 1239
Size: 268 1524
Fragments arranged by size:
1524 800 787 597 593 393 392 308
268 261
EcoNI CCTnn'n_nnAGG
Cuts at: 2249 2249
Size: 5923
EcoO109I rG'GnC_Cy
Cuts at: 2571 2857 3019 3161 2571
Size: 286 162 142 5333
Fragments arranged by size:
5333 286 162 142
EcoRI G'AATT_C
Cuts at: 3232 3610 3232
Size: 378 5545
FspI TGC'GCA
Cuts at: 4429 4429
Size: 5923
HaeII r_GCGC'y
Cuts at: 1678 2591 5070 1678
Size: 913 2479 2531
Fragments arranged by size:
2531 2479 913
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2792 2824 4564 4596 4638
Size: 32 135 32 1220 32 1740 32 42
Cuts at: 4638 4670 1373
Size: 32 2626
Fragments arranged by size:
2626 1740 1220 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2647 3267 3513 669
Size: 988 281 709 620 246 3079
Fragments arranged by size:
3079 988 709 620 281 246
HindIII A'AGCT_T
Cuts at: 748 2552 748
Size: 1804 4119
HpaI GTT'AAC
Cuts at: 3513 3513
Size: 5923
KpnI G_GTAC'C
Cuts at: 3248 3248
Size: 5923
MfeI C'AATT_G
Cuts at: 3522 3522
Size: 5923
MluI A'CGCG_T
Cuts at: 5799 5799
Size: 5923
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2530 5037 5221 1305
Size: 1225 2507 184 2007
Fragments arranged by size:
2507 2007 1225 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2145 10
Size: 54 1425 343 313 3788
Fragments arranged by size:
3788 1425 343 313 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2738 3881 4240 4399 518
Size: 648 1572 1143 359 159 2042
Fragments arranged by size:
2042 1572 1143 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2180 2203 2849 2923 3339 4016 5164 5409
Size: 574 23 646 74 416 677 1148 245
Cuts at: 5409 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 646 574 416 245 74
23
NaeI GCC'GGC
Cuts at: 2260 2260
Size: 5923
NcoI C'CATG_G
Cuts at: 513 1065 2610 513
Size: 552 1545 3826
Fragments arranged by size:
3826 1545 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5923
NgoMIV G'CCGG_C
Cuts at: 2258 2258
Size: 5923
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5923
NotI GC'GGCC_GC
Cuts at: 3370 3370
Size: 5923
NspI r_CATG'y
Cuts at: 1132 1229 3281 4826 1132
Size: 97 2052 1545 2229
Fragments arranged by size:
2229 2052 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2576 3140 1348
Size: 1228 564 4131
Fragments arranged by size:
4131 1228 564
PciI A'CATG_T
Cuts at: 4822 4822
Size: 5923
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2633 1108
Size: 1525 4398
PmeI GTTT'AAAC
Cuts at: 3227 3227
Size: 5923
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3992 4024 5505 5537 3992
Size: 32 1481 32 4378
Fragments arranged by size:
4378 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2571 3019 2571
Size: 448 5475
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5923
PsiI TTA'TAA
Cuts at: 3493 3493
Size: 5923
PspOMI G'GGCC_C
Cuts at: 3160 3160
Size: 5923
PstI C_TGCA'G
Cuts at: 830 2823 3275 830
Size: 1993 452 3478
Fragments arranged by size:
3478 1993 452
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5923
PvuII CAG'CTG
Cuts at: 2180 2203 2849 2180
Size: 23 646 5254
Fragments arranged by size:
5254 646 23
SacI G_AGCT'C
Cuts at: 2111 2111
Size: 5923
SalI G'TCGA_C
Cuts at: 1936 2645 3265 1936
Size: 709 620 4594
Fragments arranged by size:
4594 709 620
SanDI GG'GwC_CC
Cuts at: 2571 3019 2571
Size: 448 5475
SbfI CC_TGCA'GG
Cuts at: 3275 3275
Size: 5923
ScaI AGT'ACT
Cuts at: 4171 5883 4171
Size: 1712 4211
SexAI A'CCwGG_T
Cuts at: 2393 2393
Size: 5923
SfcI C'TryA_G
Cuts at: 826 1045 2819 2861 3271 4406 5087 5278 826
Size: 219 1774 42 410 1135 681 191 1471
Fragments arranged by size:
1774 1471 1135 681 410 219 191 42
SfiI GGCCn_nnn'nGGCC
Cuts at: 3142 3142
Size: 5923
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5923
SgrAI Cr'CCGG_yG
Cuts at: 1700 2709 1700
Size: 1009 4914
SmaI CCC'GGG
Cuts at: 1872 3250 1872
Size: 1378 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4027 4928 5190 820
Size: 197 926 2084 901 262 1553
Fragments arranged by size:
2084 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3714 493
Size: 3221 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5923
SphI G_CATG'C
Cuts at: 1132 3281 1132
Size: 2149 3774
SspI AAT'ATT
Cuts at: 5 52 2479 3847 5
Size: 47 2427 1368 2081
Fragments arranged by size:
2427 2081 1368 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5923
StyI C'CwwG_G
Cuts at: 513 1065 2610 3362 513
Size: 552 1545 752 3074
Fragments arranged by size:
3074 1545 752 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2640 2993 3950 3967 4120 4305 4750
Size: 1255 1060 353 957 17 153 185 445
Cuts at: 4750 325
Size: 1498
Fragments arranged by size:
1498 1255 1060 957 445 353 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4169 5881 96
Size: 274 80 33 51 1443 2192 1712 138
Fragments arranged by size:
2192 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5883 5915 5883
Size: 32 5891
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5923
XbaI T'CTAG_A
Cuts at: 3259 3259
Size: 5923
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2540 2630 2540
Size: 90 5833
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5923
XmaI C'CCGG_G
Cuts at: 1870 3248 1870
Size: 1378 4545
XmnI GAAnn'nnTTC
Cuts at: 4052 4052
Size: 5923
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2089 3731 276
Size: 53 83 186 936 555 1642 2468
Fragments arranged by size:
2468 1642 936 555 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI
KasI NarI NruI NsiI PacI PfoI PmlI PspXI
PsrI RsrII SacII SapI SfoI SrfI SwaI