Restriction map for
pFN21AE0701
(Circular) (Six-base) MAPSORT of: pFN21AE0701.seq Check: 2979 from: 1 to: 5566
pFN21AE0701 5566 bp
With 182 enzymes: SgfI *
October 20, 2009 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2236 2236
Size: 5566
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3376 278
Size: 53 83 186 936 1840 2468
Fragments arranged by size:
2468 1840 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2361 2909 1937
Size: 424 548 4594
Fragments arranged by size:
4594 548 424
Acc65I G'GTAC_C
Cuts at: 2887 2887
Size: 5566
AclI AA'CG_TT
Cuts at: 1563 2236 3693 4066 1563
Size: 673 1457 373 3063
Fragments arranged by size:
3063 1457 673 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2829 3629 5013 5281 1632
Size: 1197 800 1384 268 1917
Fragments arranged by size:
1917 1384 1197 800 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5369
AflIII A'CryG_T
Cuts at: 1203 4465 5442 1203
Size: 3262 977 1327
Fragments arranged by size:
3262 1327 977
AgeI A'CCGG_T
Cuts at: 4397 4397
Size: 5566
AhdI GACnn_n'nnGTC
Cuts at: 2839 4295 2839
Size: 1456 4110
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5566
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5566
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2045 2077 2045
Size: 32 5534
AlwNI CAG_nnn'CTG
Cuts at: 2830 3309 4881 2830
Size: 479 1572 3515
Fragments arranged by size:
3515 1572 479
ApaLI G'TGCA_C
Cuts at: 3623 4779 3623
Size: 1156 4410
ApoI r'AATT_y
Cuts at: 1493 2156 2345 2432 2875 3101 3253 5362 1493
Size: 663 189 87 443 226 152 2109 1697
Fragments arranged by size:
2109 1697 663 443 226 189 152 87
AseI AT'TA_AT
Cuts at: 160 1032 2199 4120 160
Size: 872 1167 1921 1606
Fragments arranged by size:
1921 1606 1167 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5566
AvaI C'yCGr_G
Cuts at: 1870 1943 2891 1870
Size: 73 948 4545
Fragments arranged by size:
4545 948 73
BamHI G'GATC_C
Cuts at: 2130 2896 3263 2130
Size: 766 367 4433
Fragments arranged by size:
4433 766 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2887 4342 618
Size: 325 217 627 1100 1455 1842
Fragments arranged by size:
1842 1455 1100 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2464 2885 1391
Size: 1073 421 4072
Fragments arranged by size:
4072 1073 421
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2736 2835 928
Size: 971 837 99 3659
Fragments arranged by size:
3659 971 837 99
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3757 3791 1903
Size: 34 1820 34 3678
Fragments arranged by size:
3678 1820 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3458 4668 3458
Size: 1210 4356
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5566
BfrBI ATG'CAT
Cuts at: 2723 2723
Size: 5566
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2509 4177
Size: 107 122 71 631 605 337 500 1668
Cuts at: 4177 136
Size: 1525
Fragments arranged by size:
1668 1525 631 605 500 337 122 107
71
BglII A'GATC_T
Cuts at: 5561 5561
Size: 5566
BlpI GC'TnA_GC
Cuts at: 2513 2983 2513
Size: 470 5096
Bme1580I G_kGCm'C
Cuts at: 1790 3627 4783 1790
Size: 1837 1156 2573
Fragments arranged by size:
2573 1837 1156
BmgBI CAC'GTC
Cuts at: 1318 2323 1318
Size: 1005 4561
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2136 2501 2662
Size: 416 53 83 399 24 719 365 161
Cuts at: 2662 3297 4255 442
Size: 635 958 1753
Fragments arranged by size:
1753 958 719 635 416 399 365 161
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5566
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2047 4226 1239
Size: 808 2179 2579
Fragments arranged by size:
2579 2179 808
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3691 4556 4854 3691
Size: 865 298 4403
Fragments arranged by size:
4403 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2081 4229 882
Size: 617 582 2148 2219
Fragments arranged by size:
2219 2148 617 582
BsaAI yAC'GTr
Cuts at: 493 1204 3357 493
Size: 711 2153 2702
Fragments arranged by size:
2702 2153 711
BsaBI GATnn'nnATC
Cuts at: 5560 5560
Size: 5566
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3373 3755 4390 275
Size: 53 83 186 936 1840 382 635 1451
Fragments arranged by size:
1840 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3999 4397 4671 4818 5229 3999
Size: 398 274 147 411 4336
Fragments arranged by size:
4336 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2045 2075 2370 2400 1178
Size: 30 165 30 642 30 295 30 4344
Fragments arranged by size:
4344 642 295 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2804 1186
Size: 1618 3948
BseYI C'CCAG_C
Cuts at: 2134 4769 5265 2134
Size: 2635 496 2435
Fragments arranged by size:
2635 2435 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4993
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3016 3777 4805 1540
Size: 275 186 1015 761 1028 2301
Fragments arranged by size:
2301 1028 1015 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2690 3627 3712 4783 2690
Size: 937 85 1071 3473
Fragments arranged by size:
3473 1071 937 85
BsmI GAATG_Cn'
Cuts at: 3077 3170 3077
Size: 93 5473
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2464 2690 2885 3627 3712 4783 1391
Size: 399 674 226 195 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 674 399 226 195 85
BspEI T'CCGG_A
Cuts at: 5229 5229
Size: 5566
BspHI T'CATG_A
Cuts at: 3455 5185 3455
Size: 1730 3836
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2236 2921 844
Size: 1392 685 3489
Fragments arranged by size:
3489 1392 685
BsrBI CCG'CTC
Cuts at: 1394 1823 3453 1394
Size: 429 1630 3507
Fragments arranged by size:
3507 1630 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4061 4235 65
Size: 1183 2813 174 1396
Fragments arranged by size:
2813 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4210 4397 1700
Size: 110 2400 187 2869
Fragments arranged by size:
2869 2400 187 110
BsrGI T'GTAC_A
Cuts at: 96 2568 96
Size: 2472 3094
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5566
BssSI C'ACGA_G
Cuts at: 3626 4638 3626
Size: 1012 4554
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5566
BstBI TT'CG_AA
Cuts at: 4370 4370
Size: 5566
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5566
BstYI r'GATC_y
Cuts at: 1972 2130 2850 2896 3263 3648 3665 5117 5203
Size: 158 720 46 367 385 17 1452 86
Cuts at: 5203 5215 5561 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 720 385 367 346 158 86
46 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2079 513
Size: 552 939 75 4000
Fragments arranged by size:
4000 939 552 75
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4827
Fragments arranged by size:
4827 683 56
BtsI GCAGTG_nn'
Cuts at: 2490 3082 3876 3896 2490
Size: 592 794 20 4160
Fragments arranged by size:
4160 794 592 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5566
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5531
DraI TTT'AAA
Cuts at: 2242 2870 2242
Size: 628 4938
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2407 4573 809
Size: 507 1091 2166 1802
Fragments arranged by size:
2166 1802 1091 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2010 2169 3013
Size: 54 1425 50 275 18 180 159 844
Cuts at: 3013 3902 5531 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 844 275 180 159 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3013 1537
Size: 275 1201 4090
Fragments arranged by size:
4090 1201 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3496 1353
Size: 2143 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4153 4527 4673 387
Size: 1064 138 2564 374 146 1280
Fragments arranged by size:
2564 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2047 2829 3629 4226 5013 5281 1239
Size: 393 415 782 800 597 787 268 1524
Fragments arranged by size:
1524 800 787 782 597 415 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2308 2308
Size: 5566
EcoRI G'AATT_C
Cuts at: 2345 2875 3253 2345
Size: 530 378 4658
Fragments arranged by size:
4658 530 378
FspI TGC'GCA
Cuts at: 4072 4072
Size: 5566
HaeII r_GCGC'y
Cuts at: 1678 4713 1678
Size: 3035 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2800 2825 2832 2857 4207
Size: 32 135 32 1228 25 7 25 1350
Cuts at: 4207 4239 4281 4313 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1350 1228 135 42 32 32 32
32 25 25 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2910 3156 669
Size: 988 281 972 246 3079
Fragments arranged by size:
3079 988 972 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5566
HpaI GTT'AAC
Cuts at: 3156 3156
Size: 5566
KpnI G_GTAC'C
Cuts at: 2891 2891
Size: 5566
MfeI C'AATT_G
Cuts at: 3165 3165
Size: 5566
MluI A'CGCG_T
Cuts at: 5442 5442
Size: 5566
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2692 4680 4864 1305
Size: 1387 1988 184 2007
Fragments arranged by size:
2007 1988 1387 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2171 10
Size: 54 1425 343 339 3405
Fragments arranged by size:
3405 1425 343 339 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3524 3883 4042 518
Size: 648 2358 359 159 2042
Fragments arranged by size:
2358 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2138 2177 2746 2982 3659 4807 5052 1606
Size: 532 39 569 236 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 569 532 245 236 39
NcoI C'CATG_G
Cuts at: 513 1065 2004 2079 513
Size: 552 939 75 4000
Fragments arranged by size:
4000 939 552 75
NdeI CA'TA_TG
Cuts at: 387 2701 387
Size: 2314 3252
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5566
NotI GC'GGCC_GC
Cuts at: 3013 3013
Size: 5566
NsiI A_TGCA'T
Cuts at: 2725 2725
Size: 5566
NspI r_CATG'y
Cuts at: 1132 1229 2338 2924 4469 1132
Size: 97 1109 586 1545 2229
Fragments arranged by size:
2229 1545 1109 586 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5566
PciI A'CATG_T
Cuts at: 4465 4465
Size: 5566
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2019 1108
Size: 911 4655
PfoI T'CCnGG_A
Cuts at: 2215 2215
Size: 5566
PmeI GTTT'AAAC
Cuts at: 2870 2870
Size: 5566
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2045 2077 3635 3667 5148 5180 2045
Size: 32 1558 32 1481 32 2431
Fragments arranged by size:
2431 1558 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2308 2308
Size: 5566
PshAI GACnn'nnGTC
Cuts at: 1613 2494 1613
Size: 881 4685
PsiI TTA'TAA
Cuts at: 3136 3136
Size: 5566
PstI C_TGCA'G
Cuts at: 830 2918 830
Size: 2088 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5566
PvuII CAG'CTG
Cuts at: 2138 2177 2746 2138
Size: 39 569 4958
Fragments arranged by size:
4958 569 39
SalI G'TCGA_C
Cuts at: 1936 2908 1936
Size: 972 4594
SbfI CC_TGCA'GG
Cuts at: 2918 2918
Size: 5566
ScaI AGT'ACT
Cuts at: 3814 5526 3814
Size: 1712 3854
SfcI C'TryA_G
Cuts at: 826 1045 2650 2656 2914 4049 4730 4921 826
Size: 219 1605 6 258 1135 681 191 1471
Fragments arranged by size:
1605 1471 1135 681 258 219 191 6
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5566
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5566
SmaI CCC'GGG
Cuts at: 1872 2893 1872
Size: 1021 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3670 4571 4833 820
Size: 197 926 1727 901 262 1553
Fragments arranged by size:
1727 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3357 493
Size: 2864 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5566
SphI G_CATG'C
Cuts at: 1132 2338 2924 1132
Size: 1206 586 3774
Fragments arranged by size:
3774 1206 586
SspI AAT'ATT
Cuts at: 5 52 3490 5
Size: 47 3438 2081
Fragments arranged by size:
3438 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5566
StyI C'CwwG_G
Cuts at: 513 1065 2004 2079 3005 513
Size: 552 939 75 926 3074
Fragments arranged by size:
3074 939 926 552 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3593 3610 3763 3948 4393 325
Size: 1255 2013 17 153 185 445 1498
Fragments arranged by size:
2013 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2568 3812 5524
Size: 274 80 33 51 1443 591 1244 1712
Cuts at: 5524 96
Size: 138
Fragments arranged by size:
1712 1443 1244 591 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2370 2402 5526 5558 2370
Size: 32 3124 32 2378
Fragments arranged by size:
3124 2378 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5566
XbaI T'CTAG_A
Cuts at: 2902 2902
Size: 5566
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2020 2743 2020
Size: 723 4843
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5566
XmaI C'CCGG_G
Cuts at: 1870 2891 1870
Size: 1021 4545
XmnI GAAnn'nnTTC
Cuts at: 3695 3695
Size: 5566
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3374 276
Size: 53 83 186 936 1840 2468
Fragments arranged by size:
2468 1840 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI