Restriction map for
pFN21AE0699
(Circular) (Six-base) MAPSORT of: pFN21AE0699.seq Check: 8006 from: 1 to: 18394
pFN21AE0699 18394 bp
With 182 enzymes: SgfI *
August 20, 2012 11:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 11874 16204 278
Size: 53 83 186 936 10338 4330 2468
Fragments arranged by size:
10338 4330 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6090 7167 9054 11413 13671 15737 1937
Size: 4153 1077 1887 2359 2258 2066 4594
Fragments arranged by size:
4594 4153 2359 2258 2066 1887 1077
Acc65I G'GTAC_C
Cuts at: 15715 15715
Size: 18394
AclI AA'CG_TT
Cuts at: 1563 16521 16894 1563
Size: 14958 373 3063
Fragments arranged by size:
14958 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2300 2363 3623 3877 4038 4790 5123 5702
Size: 668 63 1260 254 161 752 333 579
Cuts at: 5702 5798 6390 6823 7057 7481 7617 8321 8998
Size: 96 592 433 234 424 136 704 677
Cuts at: 8998 9414 9800 9809 10112 10496 12573 13256 13282
Size: 416 386 9 303 384 2077 683 26
Cuts at: 13282 13401 13849 13889 14885 14998 15089 16457 17841
Size: 119 448 40 996 113 91 1368 1384
Cuts at: 17841 18109 1632
Size: 268 1917
Fragments arranged by size:
2077 1917 1384 1368 1260 996 752 704
683 677 668 592 579 448 433 424
416 386 384 333 303 268 254 234
161 136 119 113 96 91 63 40
26 9
AflII C'TTAA_G
Cuts at: 820 1017 7211 8810 15257 820
Size: 197 6194 1599 6447 3957
Fragments arranged by size:
6447 6194 3957 1599 197
AflIII A'CryG_T
Cuts at: 1203 3202 9267 14514 17293 18270 1203
Size: 1999 6065 5247 2779 977 1327
Fragments arranged by size:
6065 5247 2779 1999 1327 977
AgeI A'CCGG_T
Cuts at: 17225 17225
Size: 18394
AhdI GACnn_n'nnGTC
Cuts at: 17123 17123
Size: 18394
AleI CACnn'nnGTG
Cuts at: 1166 14885 1166
Size: 13719 4675
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2354 5640 11289 14352 1184
Size: 1170 3286 5649 3063 5226
Fragments arranged by size:
5649 5226 3286 3063 1170
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4871 4903 11745 11777 4871
Size: 32 6842 32 11488
Fragments arranged by size:
11488 6842 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2998 3196 3280 3391 3595 4387 7568 9373 10042
Size: 198 84 111 204 792 3181 1805 669
Cuts at: 10042 14195 14455 14467 16137 17709 2998
Size: 4153 260 12 1670 1572 3683
Fragments arranged by size:
4153 3683 3181 1805 1670 1572 792 669
260 204 198 111 84 12
ApaLI G'TGCA_C
Cuts at: 16451 17607 16451
Size: 1156 17238
ApoI r'AATT_y
Cuts at: 1493 3741 3899 5199 5528 6290 6311 6862 7091
Size: 2248 158 1300 329 762 21 551 229
Cuts at: 7091 7253 7793 8156 8383 8665 9257 9363 9536
Size: 162 540 363 227 282 592 106 173
Cuts at: 9536 9950 10163 10223 10965 11107 11167 11305 11483
Size: 414 213 60 742 142 60 138 178
Cuts at: 11483 11745 12215 12300 12319 12380 12568 12649 13190
Size: 262 470 85 19 61 188 81 541
Cuts at: 13190 13225 13373 14546 14966 15086 15539 15703 15929
Size: 35 148 1173 420 120 453 164 226
Cuts at: 15929 16081 18190 1493
Size: 152 2109 1697
Fragments arranged by size:
2248 2109 1697 1300 1173 762 742 592
551 541 540 470 453 420 414 363
329 282 262 229 227 226 213 188
178 173 164 162 158 152 148 142
138 120 106 85 81 61 60 60
35 21 19
AseI AT'TA_AT
Cuts at: 160 1032 5693 6912 10461 11894 14982 16948 160
Size: 872 4661 1219 3549 1433 3088 1966 1606
Fragments arranged by size:
4661 3549 3088 1966 1606 1433 1219 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 18394
AvaI C'yCGr_G
Cuts at: 1870 1943 3260 6000 12522 13364 15719 1870
Size: 73 1317 2740 6522 842 2355 4545
Fragments arranged by size:
6522 4545 2740 2355 1317 842 73
AvrII C'CTAG_G
Cuts at: 11860 11860
Size: 18394
BamHI G'GATC_C
Cuts at: 4428 6526 12992 15724 16091 4428
Size: 2098 6466 2732 367 6731
Fragments arranged by size:
6731 6466 2732 2098 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2075 4409 5546 6590 7940
Size: 325 217 627 288 2334 1137 1044 1350
Cuts at: 7940 12152 14024 14411 15715 17170 618
Size: 4212 1872 387 1304 1455 1842
Fragments arranged by size:
4212 2334 1872 1842 1455 1350 1304 1137
1044 627 387 325 288 217
BanII G_rGCy'C
Cuts at: 1391 2397 3267 4466 5070 5711 6166 7238 8103
Size: 1006 870 1199 604 641 455 1072 865
Cuts at: 8103 11730 14289 15713 1391
Size: 3627 2559 1424 4072
Fragments arranged by size:
4072 3627 2559 1424 1199 1072 1006 870
865 641 604 455
BbeI G_GCGC'C
Cuts at: 2079 2079
Size: 18394
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2288 2877 2990 3519 5729 7681 9806
Size: 971 389 589 113 529 2210 1952 2125
Cuts at: 9806 12579 13422 14298 928
Size: 2773 843 876 5024
Fragments arranged by size:
5024 2773 2210 2125 1952 971 876 843
589 529 389 113
BbvCI CC'TCA_GC
Cuts at: 3268 3958 10351 14002 3268
Size: 690 6393 3651 7660
Fragments arranged by size:
7660 6393 3651 690
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2624 2658 16585 16619 1903
Size: 34 687 34 13927 34 3678
Fragments arranged by size:
13927 3678 687 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2536 5089 8755 11747 16286 17496 2536
Size: 2553 3666 2992 4539 1210 3434
Fragments arranged by size:
4539 3666 3434 2992 2553 1210
BclI T'GATC_A
Cuts at: 1548 2718 8317 8548 15234 1548
Size: 1170 5599 231 6686 4708
Fragments arranged by size:
6686 5599 4708 1170 231
BfrBI ATG'CAT
Cuts at: 6374 11648 6374
Size: 5274 13120
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3590 17005 136
Size: 107 122 71 631 605 1918 13415 1525
Fragments arranged by size:
13415 1918 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3288 3832 5929 13267 18389 3288
Size: 544 2097 7338 5122 3293
Fragments arranged by size:
7338 5122 3293 2097 544
BlpI GC'TnA_GC
Cuts at: 3338 7929 9134 12446 15811 3338
Size: 4591 1205 3312 3365 5921
Fragments arranged by size:
5921 4591 3365 3312 1205
Bme1580I G_kGCm'C
Cuts at: 1790 4414 7915 11251 12155 12608 14229 16455 17611
Size: 2624 3501 3336 904 453 1621 2226 1156
Cuts at: 17611 1790
Size: 2573
Fragments arranged by size:
3501 3336 2624 2573 2226 1621 1156 904
453
BmgBI CAC'GTC
Cuts at: 1318 14386 1318
Size: 13068 5326
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3450 4174 6338
Size: 416 53 83 399 24 2033 724 2164
Cuts at: 6338 7142 7785 11067 11238 12819 13468 14281 14427
Size: 804 643 3282 171 1581 649 813 146
Cuts at: 14427 16125 17083 442
Size: 1698 958 1753
Fragments arranged by size:
3282 2164 2033 1753 1698 1581 958 813
804 724 649 643 416 399 171 146
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 18394
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3059 4349 13615 13690 3059
Size: 1290 9266 75 7763
Fragments arranged by size:
9266 7763 1290 75
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2270 2975 3173 3498 4364 4545 4578 4605
Size: 1031 705 198 325 866 181 33 27
Cuts at: 4605 4757 5054 5777 5908 6246 7025 7908 10480
Size: 152 297 723 131 338 779 883 2572
Cuts at: 10480 10625 12678 14082 15654 17054 1239
Size: 145 2053 1404 1572 1400 2579
Fragments arranged by size:
2579 2572 2053 1572 1404 1400 1031 883
866 779 723 705 338 325 297 198
181 152 145 131 33 27
Bpu10I CC'TnA_GC
Cuts at: 3268 3958 7575 10351 13578 14002 15423 3268
Size: 690 3617 2776 3227 424 1421 6239
Fragments arranged by size:
6239 3617 3227 2776 1421 690 424
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2381 3506 5100 6896 7937 8966 11927 14489 14526
Size: 1125 1594 1796 1041 1029 2961 2562 37
Cuts at: 14526 14858 15102 15298 16519 17384 17682 2381
Size: 332 244 196 1221 865 298 3093
Fragments arranged by size:
3093 2961 2562 1796 1594 1221 1125 1041
1029 865 332 298 244 196 37
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2619 4789 4843 6282 8222 9558 17057
Size: 617 1120 2170 54 1439 1940 1336 7499
Cuts at: 17057 882
Size: 2219
Fragments arranged by size:
7499 2219 2170 1940 1439 1336 1120 617
54
BsaAI yAC'GTr
Cuts at: 493 1204 5680 14894 16185 493
Size: 711 4476 9214 1291 2702
Fragments arranged by size:
9214 4476 2702 1291 711
BsaBI GATnn'nnATC
Cuts at: 10077 13962 15605 18388 10077
Size: 3885 1643 2783 10083
Fragments arranged by size:
10083 3885 2783 1643
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2076 11871 16201 16583
Size: 53 83 186 936 543 9795 4330 382
Cuts at: 16583 17218 275
Size: 635 1451
Fragments arranged by size:
9795 4330 1451 936 635 543 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2151 4678 5203 12915 16827 17225 17499 17646 18057
Size: 2527 525 7712 3912 398 274 147 411
Cuts at: 18057 2151
Size: 2488
Fragments arranged by size:
7712 3912 2527 2488 525 411 398 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2941 2971 4943 4958 4973
Size: 30 165 30 1538 30 1972 15 15
Cuts at: 4973 4988 5430 5460 5949 5979 7832 7862 10611
Size: 15 442 30 489 30 1853 30 2749
Cuts at: 10611 10641 11422 11452 13344 13374 13690 13720 1178
Size: 30 781 30 1892 30 316 30 5852
Fragments arranged by size:
5852 2749 1972 1892 1853 1538 781 489
442 316 165 30 30 30 30 30
30 30 30 30 30 15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2378 3718 4364 6565 10293 11427 1186
Size: 1192 1340 646 2201 3728 1134 8153
Fragments arranged by size:
8153 3728 2201 1340 1192 1134 646
BseYI C'CCAG_C
Cuts at: 2259 3952 4760 7622 10986 11127 11250 13945 14014
Size: 1693 808 2862 3364 141 123 2695 69
Cuts at: 14014 17597 18093 2259
Size: 3583 496 2560
Fragments arranged by size:
3583 3364 2862 2695 2560 1693 808 496
141 123 69
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3421 4915 7908 13641 13890 15320 1163
Size: 573 1685 1494 2993 5733 249 1430 4237
Fragments arranged by size:
5733 4237 2993 1685 1494 1430 573 249
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 15844 16605 17633 1540
Size: 275 186 13843 761 1028 2301
Fragments arranged by size:
13843 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2397 2942 3176 5070 5711 6925 7652 8649 11730
Size: 545 234 1894 641 1214 727 997 3081
Cuts at: 11730 12581 12699 16455 16540 17611 2397
Size: 851 118 3756 85 1071 3180
Fragments arranged by size:
3756 3180 3081 1894 1214 1071 997 851
727 641 545 234 118 85
BsmI GAATG_Cn'
Cuts at: 4510 7024 11554 11810 12877 15905 15998 4510
Size: 2514 4530 256 1067 3028 93 6906
Fragments arranged by size:
6906 4530 3028 2514 1067 256 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2397 2942 3176 3267 4414 4466 5070
Size: 399 607 545 234 91 1147 52 604
Cuts at: 5070 5711 6166 6925 7238 7652 7915 8103 8649
Size: 641 455 759 313 414 263 188 546
Cuts at: 8649 11251 11730 12155 12581 12608 12699 14229 14289
Size: 2602 479 425 426 27 91 1530 60
Cuts at: 14289 15713 16455 16540 17611 1391
Size: 1424 742 85 1071 2174
Fragments arranged by size:
2602 2174 1530 1424 1147 1071 759 742
641 607 604 546 545 479 455 426
425 414 399 313 263 234 188 91
91 85 60 52 27
BspEI T'CCGG_A
Cuts at: 2151 4678 5203 12915 18057 2151
Size: 2527 525 7712 5142 2488
Fragments arranged by size:
7712 5142 2527 2488 525
BspHI T'CATG_A
Cuts at: 7834 15645 16283 18013 7834
Size: 7811 638 1730 8215
Fragments arranged by size:
8215 7811 1730 638
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2413 6207 10355 10728 13627 15749 844
Size: 1569 3794 4148 373 2899 2122 3489
Fragments arranged by size:
4148 3794 3489 2899 2122 1569 373
BsrBI CCG'CTC
Cuts at: 1394 1823 16281 1394
Size: 429 14458 3507
Fragments arranged by size:
14458 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2602 3399 4563 5709 7278 7690 7750
Size: 1183 1354 797 1164 1146 1569 412 60
Cuts at: 7750 9145 12984 13506 15447 16889 17063 65
Size: 1395 3839 522 1941 1442 174 1396
Fragments arranged by size:
3839 1941 1569 1442 1396 1395 1354 1183
1164 1146 797 522 412 174 60
BsrFI r'CCGG_y
Cuts at: 1700 1810 6540 17038 17225 1700
Size: 110 4730 10498 187 2869
Fragments arranged by size:
10498 4730 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 6102 13131 96
Size: 6006 7029 5359
Fragments arranged by size:
7029 6006 5359
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 18394
BssSI C'ACGA_G
Cuts at: 4724 7865 9561 16454 17466 4724
Size: 3141 1696 6893 1012 5652
Fragments arranged by size:
6893 5652 3141 1696 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5126 13655 15334 794
Size: 4332 8529 1679 3854
Fragments arranged by size:
8529 4332 3854 1679
BstBI TT'CG_AA
Cuts at: 6302 14702 17198 6302
Size: 8400 2496 7498
Fragments arranged by size:
8400 7498 2496
BstEII G'GTnAC_C
Cuts at: 1184 3235 7478 1184
Size: 2051 4243 12100
Fragments arranged by size:
12100 4243 2051
BstXI CCAn_nnnn'nTGG
Cuts at: 4442 5497 7159 4442
Size: 1055 1662 15677
Fragments arranged by size:
15677 1662 1055
BstYI r'GATC_y
Cuts at: 1972 3288 3832 4428 5412 5590 5929 6221 6526
Size: 1316 544 596 984 178 339 292 305
Cuts at: 6526 7423 10087 12992 13267 13519 15132 15724 16091
Size: 897 2664 2905 275 252 1613 592 367
Cuts at: 16091 16476 16493 17945 18031 18043 18389 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
2905 2664 1977 1613 1452 1316 984 897
596 592 544 385 367 346 339 305
292 275 252 178 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2325 4936 6563 13010 2325
Size: 2611 1627 6447 7709
Fragments arranged by size:
7709 6447 2611 1627
BtgI C'CryG_G
Cuts at: 513 1065 2004 3498 3749 6868 12003 12375 12782
Size: 552 939 1494 251 3119 5135 372 407
Cuts at: 12782 14028 513
Size: 1246 4879
Fragments arranged by size:
5135 4879 3119 1494 1246 939 552 407
372 251
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5862 7603 487
Size: 683 56 4636 1741 11278
Fragments arranged by size:
11278 4636 1741 683 56
BtsI GCAGTG_nn'
Cuts at: 2642 4035 6791 7670 9444 10064 10383 12131 15417
Size: 1393 2756 879 1774 620 319 1748 3286
Cuts at: 15417 15910 16704 16724 2642
Size: 493 794 20 4312
Fragments arranged by size:
4312 3286 2756 1774 1748 1393 879 794
620 493 319 20
ClaI AT'CG_AT
Cuts at: 1554 7725 1554
Size: 6171 12223
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5318 5353 10554 10589 13072 13107 529
Size: 35 4754 35 5201 35 2483 35 5816
Fragments arranged by size:
5816 5201 4754 2483 35 35 35 35
DraI TTT'AAA
Cuts at: 6714 7395 9232 11079 12034 14857 15040 15698 6714
Size: 681 1837 1847 955 2823 183 658 9410
Fragments arranged by size:
9410 2823 1847 1837 955 681 658 183
DraIII CAC_nnn'GTG
Cuts at: 13859 13859
Size: 18394
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 14686 17401 809
Size: 507 13370 2715 1802
Fragments arranged by size:
13370 2715 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2403 5851 6538
Size: 54 1425 50 275 18 573 3448 687
Cuts at: 6538 6946 7066 10753 12853 15841 16730 18359 8
Size: 408 120 3687 2100 2988 889 1629 43
Fragments arranged by size:
3687 3448 2988 2100 1629 1425 889 687
573 408 275 120 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 15841 1537
Size: 275 14029 4090
Fragments arranged by size:
14029 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2082 2807 3050 3942 5511 5840 6487 7309
Size: 729 725 243 892 1569 329 647 822
Cuts at: 7309 7537 9828 11958 12373 14178 16324 1353
Size: 228 2291 2130 415 1805 2146 3423
Fragments arranged by size:
3423 2291 2146 2130 1805 1569 892 822
729 725 647 415 329 243 228
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4960 6103 16981 17355 17501 387
Size: 1064 138 3371 1143 10878 374 146 1280
Fragments arranged by size:
10878 3371 1280 1143 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2395 5068 5709 11728 2395
Size: 2673 641 6019 9061
Fragments arranged by size:
9061 6019 2673 641
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2270 2300 2363 2975 3173 3498 3623
Size: 393 638 30 63 612 198 325 125
Cuts at: 3623 3877 4038 4364 4545 4578 4605 4757 4790
Size: 254 161 326 181 33 27 152 33
Cuts at: 4790 5054 5123 5702 5777 5798 5908 6246 6390
Size: 264 69 579 75 21 110 338 144
Cuts at: 6390 6823 7025 7057 7481 7617 7908 8321 8998
Size: 433 202 32 424 136 291 413 677
Cuts at: 8998 9414 9800 9809 10112 10480 10496 10625 12573
Size: 416 386 9 303 368 16 129 1948
Cuts at: 12573 12678 13256 13282 13401 13849 13889 14082 14885
Size: 105 578 26 119 448 40 193 803
Cuts at: 14885 14998 15089 15654 16457 17054 17841 18109 1239
Size: 113 91 565 803 597 787 268 1524
Fragments arranged by size:
1948 1524 803 803 787 677 638 612
597 579 578 565 448 433 424 416
413 393 386 368 338 326 325 303
291 268 264 254 202 198 193 181
161 152 144 136 129 125 119 113
110 105 91 75 69 63 40 33
33 32 30 27 26 21 16 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2303 11617 12854 2303
Size: 9314 1237 7843
Fragments arranged by size:
9314 7843 1237
EcoO109I rG'GnC_Cy
Cuts at: 3381 10859 11736 12727 14242 14421 14905 3381
Size: 7478 877 991 1515 179 484 6870
Fragments arranged by size:
7478 6870 1515 991 877 484 179
EcoRI G'AATT_C
Cuts at: 8383 10163 10223 11483 12568 14546 15703 16081 8383
Size: 1780 60 1260 1085 1978 1157 378 10696
Fragments arranged by size:
10696 1978 1780 1260 1157 1085 378 60
EcoRV GAT'ATC
Cuts at: 3826 12625 14380 3826
Size: 8799 1755 7840
Fragments arranged by size:
8799 7840 1755
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3995 4492 4927 7226 8102 9830 11227 11503 12466
Size: 497 435 2299 876 1728 1397 276 963
Cuts at: 12466 3995
Size: 9923
Fragments arranged by size:
9923 2299 1728 1397 963 876 497 435
276
FspI TGC'GCA
Cuts at: 10724 16900 10724
Size: 6176 12218
HaeII r_GCGC'y
Cuts at: 1678 2036 2045 2079 4944 17541 1678
Size: 358 9 34 2865 12597 2531
Fragments arranged by size:
12597 2865 2531 358 34 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3025 3057 3691 3723 3941
Size: 32 135 32 1453 32 634 32 218
Cuts at: 3941 3973 4178 4210 4286 4318 4437 4469 4852
Size: 32 205 32 76 32 119 32 383
Cuts at: 4852 4884 4958 4990 6809 6841 6944 6976 7200
Size: 32 74 32 1819 32 103 32 224
Cuts at: 7200 7232 8189 8221 9009 9041 10547 10579 10611
Size: 32 957 32 788 32 1506 32 32
Cuts at: 10611 10624 10643 10656 13090 13122 14308 14340 14786
Size: 13 19 13 2434 32 1186 32 446
Cuts at: 14786 14818 15214 15246 15506 15538 17035 17067 17109
Size: 32 396 32 260 32 1497 32 42
Cuts at: 17109 17141 1373
Size: 32 2626
Fragments arranged by size:
2626 2434 1819 1506 1497 1453 1186 957
788 634 446 396 383 260 224 218
205 135 119 103 76 74 42 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 19
13 13
HincII GTy'rAC
Cuts at: 669 1657 1938 2712 3352 3510 4150 5218 6196
Size: 988 281 774 640 158 640 1068 978
Cuts at: 6196 7168 9253 11196 12436 13395 13464 14260 14472
Size: 972 2085 1943 1240 959 69 796 212
Cuts at: 14472 15738 15984 669
Size: 1266 246 3079
Fragments arranged by size:
3079 2085 1943 1266 1240 1068 988 978
972 959 796 774 640 640 281 246
212 158 69
HindIII A'AGCT_T
Cuts at: 748 4155 9212 11945 12443 15111 15662 748
Size: 3407 5057 2733 498 2668 551 3480
Fragments arranged by size:
5057 3480 3407 2733 2668 551 498
HpaI GTT'AAC
Cuts at: 3510 9253 11196 13395 13464 15984 3510
Size: 5743 1943 2199 69 2520 5920
Fragments arranged by size:
5920 5743 2520 2199 1943 69
KasI G'GCGC_C
Cuts at: 2075 2075
Size: 18394
KpnI G_GTAC'C
Cuts at: 15719 15719
Size: 18394
MfeI C'AATT_G
Cuts at: 8163 8392 8903 9484 9990 10251 15993 8163
Size: 229 511 581 506 261 5742 10564
Fragments arranged by size:
10564 5742 581 511 506 261 229
MluI A'CGCG_T
Cuts at: 18270 18270
Size: 18394
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3902 4974 6582 9166 12697 14950 17508 17692
Size: 2597 1072 1608 2584 3531 2253 2558 184
Cuts at: 17692 1305
Size: 2007
Fragments arranged by size:
3531 2597 2584 2558 2253 2007 1608 1072
184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2405 6948 10755 12855 10
Size: 54 1425 343 573 4543 3807 2100 5549
Fragments arranged by size:
5549 4543 3807 2100 1425 573 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4496 5511 5622 6265 7521 7643 7737
Size: 648 3330 1015 111 643 1256 122 94
Cuts at: 7737 8658 10524 10792 11079 11801 13110 13571 14039
Size: 921 1866 268 287 722 1309 461 468
Cuts at: 14039 14885 15644 16352 16711 16870 518
Size: 846 759 708 359 159 2042
Fragments arranged by size:
3330 2042 1866 1309 1256 1015 921 846
759 722 708 648 643 468 461 359
287 268 159 122 111 94
MspA1I CmG'CkG
Cuts at: 1606 3112 3280 4058 4987 7783 7928 10468 14953
Size: 1506 168 778 929 2796 145 2540 4485
Cuts at: 14953 15810 16487 17635 17880 1606
Size: 857 677 1148 245 2120
Fragments arranged by size:
4485 2796 2540 2120 1506 1148 929 857
778 677 245 168 145
NaeI GCC'GGC
Cuts at: 6542 6542
Size: 18394
NarI GG'CG_CC
Cuts at: 2076 2076
Size: 18394
NcoI C'CATG_G
Cuts at: 513 1065 2004 3498 3749 6868 12003 12375 12782
Size: 552 939 1494 251 3119 5135 372 407
Cuts at: 12782 513
Size: 6125
Fragments arranged by size:
6125 5135 3119 1494 939 552 407 372
251
NdeI CA'TA_TG
Cuts at: 387 5619 6238 6505 7240 14553 15031 387
Size: 5232 619 267 735 7313 478 3750
Fragments arranged by size:
7313 5232 3750 735 619 478 267
NgoMIV G'CCGG_C
Cuts at: 6540 6540
Size: 18394
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 18394
NotI GC'GGCC_GC
Cuts at: 15841 15841
Size: 18394
NsiI A_TGCA'T
Cuts at: 6376 11650 6376
Size: 5274 13120
NspI r_CATG'y
Cuts at: 1132 1229 2771 3212 4021 4612 7472 8417 8954
Size: 97 1542 441 809 591 2860 945 537
Cuts at: 8954 9271 10994 11845 13755 15752 17297 1132
Size: 317 1723 851 1910 1997 1545 2229
Fragments arranged by size:
2860 2229 1997 1910 1723 1545 1542 945
851 809 591 537 441 317 97
PasI CC'CwG_GG
Cuts at: 1348 2198 8347 1348
Size: 850 6149 11395
Fragments arranged by size:
11395 6149 850
PciI A'CATG_T
Cuts at: 9267 17293 9267
Size: 8026 10368
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3499 4387 5800 7990 10924 10993 12862 1108
Size: 2391 888 1413 2190 2934 69 1869 6640
Fragments arranged by size:
6640 2934 2391 2190 1869 1413 888 69
PfoI T'CCnGG_A
Cuts at: 2991 3664 4314 4570 5637 8744 11749 13740 14139
Size: 673 650 256 1067 3107 3005 1991 399
Cuts at: 14139 15670 2991
Size: 1531 5715
Fragments arranged by size:
5715 3107 3005 1991 1531 1067 673 650
399 256
PmeI GTTT'AAAC
Cuts at: 15698 15698
Size: 18394
PmlI CAC'GTG
Cuts at: 5680 5680
Size: 18394
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 9740 9772 16463 16495 17976 18008 9740
Size: 32 6691 32 1481 32 10126
Fragments arranged by size:
10126 6691 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3381 10859 11736 14242 14421 14905 3381
Size: 7478 877 2506 179 484 6870
Fragments arranged by size:
7478 6870 2506 877 484 179
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 18394
PsiI TTA'TAA
Cuts at: 7344 8969 15964 7344
Size: 1625 6995 9774
Fragments arranged by size:
9774 6995 1625
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 14063 14095 14063
Size: 32 18362
PstI C_TGCA'G
Cuts at: 830 2270 2377 4425 8529 8805 10059 12138 12603
Size: 1440 107 2048 4104 276 1254 2079 465
Cuts at: 12603 14331 15746 830
Size: 1728 1415 3478
Fragments arranged by size:
4104 3478 2079 2048 1728 1440 1415 1254
465 276 107
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 18394
PvuII CAG'CTG
Cuts at: 3112 3280 4058 7783 7928 14953 3112
Size: 168 778 3725 145 7025 6553
Fragments arranged by size:
7025 6553 3725 778 168 145
SacI G_AGCT'C
Cuts at: 2397 5070 5711 11730 2397
Size: 2673 641 6019 9061
Fragments arranged by size:
9061 6019 2673 641
SalI G'TCGA_C
Cuts at: 1936 7166 15736 1936
Size: 5230 8570 4594
Fragments arranged by size:
8570 5230 4594
SanDI GG'GwC_CC
Cuts at: 3381 3381
Size: 18394
SapI GCTCTTCn'nnn_
Cuts at: 9828 9828
Size: 18394
SbfI CC_TGCA'GG
Cuts at: 15746 15746
Size: 18394
ScaI AGT'ACT
Cuts at: 8737 12241 16642 18354 8737
Size: 3504 4401 1712 8777
Fragments arranged by size:
8777 4401 3504 1712
SexAI A'CCwGG_T
Cuts at: 3243 3243
Size: 18394
SfcI C'TryA_G
Cuts at: 826 1045 2266 2373 3375 4421 5025 5466 6091
Size: 219 1221 107 1002 1046 604 441 625
Cuts at: 6091 6121 7367 8525 8801 9455 10055 12134 12599
Size: 30 1246 1158 276 654 600 2079 465
Cuts at: 12599 14080 14327 15742 16877 17558 17749 826
Size: 1481 247 1415 1135 681 191 1471
Fragments arranged by size:
2079 1481 1471 1415 1246 1221 1158 1135
1046 1002 681 654 625 604 600 465
441 276 247 219 191 107 30
SfoI GGC'GCC
Cuts at: 2077 2077
Size: 18394
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 18394
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 18394
SmaI CCC'GGG
Cuts at: 1872 15721 1872
Size: 13849 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2396 3521 5115 6000 6875 7211
Size: 197 926 453 1125 1594 885 875 336
Cuts at: 7211 7952 8810 8945 11942 14504 14541 14873 15117
Size: 741 858 135 2997 2562 37 332 244
Cuts at: 15117 15257 15313 16498 17399 17661 820
Size: 140 56 1185 901 262 1553
Fragments arranged by size:
2997 2562 1594 1553 1185 1125 926 901
885 875 858 741 453 336 332 262
244 197 140 135 56 37
SnaBI TAC'GTA
Cuts at: 493 14894 16185 493
Size: 14401 1291 2702
Fragments arranged by size:
14401 2702 1291
SpeI A'CTAG_T
Cuts at: 152 152
Size: 18394
SphI G_CATG'C
Cuts at: 1132 3212 4021 11845 15752 1132
Size: 2080 809 7824 3907 3774
Fragments arranged by size:
7824 3907 3774 2080 809
SspI AAT'ATT
Cuts at: 5 52 6366 8243 8314 8605 8710 9707 12013
Size: 47 6314 1877 71 291 105 997 2306
Cuts at: 12013 12530 13403 15167 15203 15346 16318 5
Size: 517 873 1764 36 143 972 2081
Fragments arranged by size:
6314 2306 2081 1877 1764 997 972 873
517 291 143 105 71 47 36
StuI AGG'CCT
Cuts at: 1517 3075 5023 1517
Size: 1558 1948 14888
Fragments arranged by size:
14888 1948 1558
StyI C'CwwG_G
Cuts at: 513 1065 2004 3147 3498 3749 4578 5088 6586
Size: 552 939 1143 351 251 829 510 1498
Cuts at: 6586 6868 8418 9423 9784 11860 12003 12375 12782
Size: 282 1550 1005 361 2076 143 372 407
Cuts at: 12782 12850 14229 15833 513
Size: 68 1379 1604 3074
Fragments arranged by size:
3074 2076 1604 1550 1498 1379 1143 1005
939 829 552 510 407 372 361 351
282 251 143 68
SwaI ATTT'AAAT
Cuts at: 11079 11079
Size: 18394
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4397 6053 7151 9582 16421 16438 16591
Size: 1255 2817 1656 1098 2431 6839 17 153
Cuts at: 16591 16776 17221 325
Size: 185 445 1498
Fragments arranged by size:
6839 2817 2431 1656 1498 1255 1098 445
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2157 2742 3551
Size: 274 80 33 51 1443 180 585 809
Cuts at: 3551 4161 6102 8275 8735 11295 11700 12138 12239
Size: 610 1941 2173 460 2560 405 438 101
Cuts at: 12239 12411 13131 16640 18352 96
Size: 172 720 3509 1712 138
Fragments arranged by size:
3509 2560 2173 1941 1712 1443 809 720
610 585 460 438 405 274 180 172
138 101 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2941 2973 3555 3587 5669 5701 5949 5981 9965
Size: 32 582 32 2082 32 248 32 3984
Cuts at: 9965 9997 11422 11454 11540 11572 18354 18386 2941
Size: 32 1425 32 86 32 6782 32 2949
Fragments arranged by size:
6782 3984 2949 2082 1425 582 248 86
32 32 32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4145 5182 1316
Size: 2829 1037 14528
Fragments arranged by size:
14528 2829 1037
XbaI T'CTAG_A
Cuts at: 6070 6295 9550 15730 6070
Size: 225 3255 6180 8734
Fragments arranged by size:
8734 6180 3255 225
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5008 5584 8846 12262 5008
Size: 576 3262 3416 11140
Fragments arranged by size:
11140 3416 3262 576
XhoI C'TCGA_G
Cuts at: 1943 6000 1943
Size: 4057 14337
XmaI C'CCGG_G
Cuts at: 1870 15719 1870
Size: 13849 4545
XmnI GAAnn'nnTTC
Cuts at: 3423 5783 6254 8387 11450 11483 13902 15090 16523
Size: 2360 471 2133 3063 33 2419 1188 1433
Cuts at: 16523 3423
Size: 5294
Fragments arranged by size:
5294 3063 2419 2360 2133 1433 1188 471
33
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 11872 16202 276
Size: 53 83 186 936 10338 4330 2468
Fragments arranged by size:
10338 4330 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PsrI PstI PvuI PvuII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BaeI BsiWI BsmBI BstZ17I
FseI FspAI NruI PacI PspOMI PspXI RsrII SacII
SfiI SrfI