Restriction map for
pFN21AE0698
(Circular) (Six-base) MAPSORT of: pFN21AE0698.seq Check: 8537 from: 1 to: 6994
pFN21AE0698 6994 bp
With 182 enzymes: SgfI *
February 26, 2010 09:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4804 278
Size: 53 83 186 936 3268 2468
Fragments arranged by size:
3268 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4337 1937
Size: 2400 4594
Acc65I G'GTAC_C
Cuts at: 4315 4315
Size: 6994
AclI AA'CG_TT
Cuts at: 1563 5121 5494 1563
Size: 3558 373 3063
Fragments arranged by size:
3558 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2603 4142 4199 5057 6441 6709 1632
Size: 971 1539 57 858 1384 268 1917
Fragments arranged by size:
1917 1539 1384 971 858 268 57
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6797
AflIII A'CryG_T
Cuts at: 1203 3187 5893 6870 1203
Size: 1984 2706 977 1327
Fragments arranged by size:
2706 1984 1327 977
AgeI A'CCGG_T
Cuts at: 5825 5825
Size: 6994
AhdI GACnn_n'nnGTC
Cuts at: 5723 5723
Size: 6994
AleI CACnn'nnGTG
Cuts at: 1166 2567 3113 1166
Size: 1401 546 5047
Fragments arranged by size:
5047 1401 546
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3740 1184
Size: 2556 4438
AlwNI CAG_nnn'CTG
Cuts at: 2965 2983 4036 4737 6309 2965
Size: 18 1053 701 1572 3650
Fragments arranged by size:
3650 1572 1053 701 18
ApaLI G'TGCA_C
Cuts at: 5051 6207 5051
Size: 1156 5838
ApoI r'AATT_y
Cuts at: 1493 2294 2505 2708 3295 3954 4303 4529 4681
Size: 801 211 203 587 659 349 226 152
Cuts at: 4681 6790 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 801 659 587 349 226 211
203 152
AseI AT'TA_AT
Cuts at: 160 1032 3486 4275 5548 160
Size: 872 2454 789 1273 1606
Fragments arranged by size:
2454 1606 1273 872 789
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6994
AvaI C'yCGr_G
Cuts at: 1870 1943 2182 4319 1870
Size: 73 239 2137 4545
Fragments arranged by size:
4545 2137 239 73
BamHI G'GATC_C
Cuts at: 4324 4691 4324
Size: 367 6627
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2792 3826 4315 5770 618
Size: 325 217 627 1005 1034 489 1455 1842
Fragments arranged by size:
1842 1455 1034 1005 627 489 325 217
BanII G_rGCy'C
Cuts at: 1391 2120 2925 4313 1391
Size: 729 805 1388 4072
Fragments arranged by size:
4072 1388 805 729
BbeI G_GCGC'C
Cuts at: 2796 2796
Size: 6994
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2541 2744 3111 3668 3941 928
Size: 971 642 203 367 557 273 3981
Fragments arranged by size:
3981 971 642 557 367 273 203
BbvCI CC'TCA_GC
Cuts at: 3681 3681
Size: 6994
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5185 5219 1903
Size: 34 3248 34 3678
Fragments arranged by size:
3678 3248 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4886 6096 4886
Size: 1210 5784
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6994
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5605 136
Size: 107 122 71 631 605 3933 1525
Fragments arranged by size:
3933 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3068 6989 3068
Size: 3921 3073
BlpI GC'TnA_GC
Cuts at: 2976 4411 2976
Size: 1435 5559
Bme1580I G_kGCm'C
Cuts at: 1790 2633 5055 6211 1790
Size: 843 2422 1156 2573
Fragments arranged by size:
2573 2422 1156 843
BmgBI CAC'GTC
Cuts at: 1318 4000 1318
Size: 2682 4312
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3256 4725 5683
Size: 416 53 83 399 24 1839 1469 958
Cuts at: 5683 442
Size: 1753
Fragments arranged by size:
1839 1753 1469 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6994
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2595 3667 2595
Size: 1072 5922
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2123 3606 3888 3965 5654 1239
Size: 884 1483 282 77 1689 2579
Fragments arranged by size:
2579 1689 1483 884 282 77
Bpu10I CC'TnA_GC
Cuts at: 3681 3681
Size: 6994
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3525 4088 5119 5984 6282 3525
Size: 563 1031 865 298 4237
Fragments arranged by size:
4237 1031 865 563 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3318 5657 882
Size: 617 1819 2339 2219
Fragments arranged by size:
2339 2219 1819 617
BsaAI yAC'GTr
Cuts at: 493 1204 2437 3188 4785 493
Size: 711 1233 751 1597 2702
Fragments arranged by size:
2702 1597 1233 751 711
BsaBI GATnn'nnATC
Cuts at: 2484 2604 6988 2484
Size: 120 4384 2490
Fragments arranged by size:
4384 2490 120
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2793 4801 5183 5818
Size: 53 83 186 936 1260 2008 382 635
Cuts at: 5818 275
Size: 1451
Fragments arranged by size:
2008 1451 1260 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2892 3310 4133 5427 5825 6099 6246 6657 2892
Size: 418 823 1294 398 274 147 411 3229
Fragments arranged by size:
3229 1294 823 418 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2134 2164 1178
Size: 30 165 30 731 30 6008
Fragments arranged by size:
6008 731 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2297 4184 1186
Size: 1111 1887 3996
Fragments arranged by size:
3996 1887 1111
BseYI C'CCAG_C
Cuts at: 2784 3018 3407 4044 6197 6693 2784
Size: 234 389 637 2153 496 3085
Fragments arranged by size:
3085 2153 637 496 389 234
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2897 1163
Size: 573 1161 5260
Fragments arranged by size:
5260 1161 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4444 5205 6233 1540
Size: 275 186 2443 761 1028 2301
Fragments arranged by size:
2443 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5055 5140 6211 5055
Size: 85 1071 5838
Fragments arranged by size:
5838 1071 85
BsmI GAATG_Cn'
Cuts at: 2581 3367 4505 4598 2581
Size: 786 1138 93 4977
Fragments arranged by size:
4977 1138 786 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2120 2633 2925 4313 5055 5140 6211
Size: 399 330 513 292 1388 742 85 1071
Cuts at: 6211 1391
Size: 2174
Fragments arranged by size:
2174 1388 1071 742 513 399 330 292
85
BspEI T'CCGG_A
Cuts at: 2892 6657 2892
Size: 3765 3229
BspHI T'CATG_A
Cuts at: 4883 6613 4883
Size: 1730 5264
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2919 4349 844
Size: 2075 1430 3489
Fragments arranged by size:
3489 2075 1430
BsrBI CCG'CTC
Cuts at: 1394 1823 4881 1394
Size: 429 3058 3507
Fragments arranged by size:
3507 3058 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2345 5489 5663 65
Size: 1183 1097 3144 174 1396
Fragments arranged by size:
3144 1396 1183 1097 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5638 5825 1700
Size: 110 3828 187 2869
Fragments arranged by size:
3828 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6994
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6994
BssSI C'ACGA_G
Cuts at: 5054 6066 5054
Size: 1012 5982
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2167 3551 3560 794
Size: 1373 1384 9 4228
Fragments arranged by size:
4228 1384 1373 9
BstBI TT'CG_AA
Cuts at: 5798 5798
Size: 6994
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6994
BstYI r'GATC_y
Cuts at: 1972 3068 3613 4324 4691 5076 5093 6545 6631
Size: 1096 545 711 367 385 17 1452 86
Cuts at: 6631 6643 6989 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1096 711 545 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2207 2207
Size: 6994
BtgI C'CryG_G
Cuts at: 513 1065 2004 2872 3375 3733 3881 3891 4111
Size: 552 939 868 503 358 148 10 220
Cuts at: 4111 513
Size: 3396
Fragments arranged by size:
3396 939 868 552 503 358 220 148
10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3159 487
Size: 683 56 1933 4322
Fragments arranged by size:
4322 1933 683 56
BtsI GCAGTG_nn'
Cuts at: 3006 4510 5304 5324 3006
Size: 1504 794 20 4676
Fragments arranged by size:
4676 1504 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6994
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6959
DraI TTT'AAA
Cuts at: 2175 4298 2175
Size: 2123 4871
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6001 809
Size: 507 4685 1802
Fragments arranged by size:
4685 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2619 2812 3372
Size: 54 1425 50 275 18 789 193 560
Cuts at: 3372 3941 4441 5330 6959 8
Size: 569 500 889 1629 43
Fragments arranged by size:
1629 1425 889 789 569 560 500 275
193 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4441 1537
Size: 275 2629 4090
Fragments arranged by size:
4090 2629 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2122 2907 3234 4014 4161 4185 4239 4924
Size: 769 785 327 780 147 24 54 685
Cuts at: 4924 1353
Size: 3423
Fragments arranged by size:
3423 785 780 769 685 327 147 54
24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5581 5955 6101 387
Size: 1064 138 3992 374 146 1280
Fragments arranged by size:
3992 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2123 2603 3606 3888 3965 4142 4199
Size: 393 491 480 1003 282 77 177 57
Cuts at: 4199 5057 5654 6441 6709 1239
Size: 858 597 787 268 1524
Fragments arranged by size:
1524 1003 858 787 597 491 480 393
282 268 177 77 57
EcoNI CCTnn'n_nnAGG
Cuts at: 3233 3233
Size: 6994
EcoO109I rG'GnC_Cy
Cuts at: 3244 3380 3866 3244
Size: 136 486 6372
Fragments arranged by size:
6372 486 136
EcoRI G'AATT_C
Cuts at: 4303 4681 4303
Size: 378 6616
FspI TGC'GCA
Cuts at: 2862 5500 2862
Size: 2638 4356
HaeII r_GCGC'y
Cuts at: 1678 2796 6141 1678
Size: 1118 3345 2531
Fragments arranged by size:
3345 2531 1118
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3150 3182 3853 3885 5635
Size: 32 135 32 1578 32 671 32 1750
Cuts at: 5635 5667 5709 5741 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1750 1578 671 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2956 4338 4584 669
Size: 988 281 1018 1382 246 3079
Fragments arranged by size:
3079 1382 1018 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6994
HpaI GTT'AAC
Cuts at: 4584 4584
Size: 6994
KasI G'GCGC_C
Cuts at: 2792 2792
Size: 6994
KpnI G_GTAC'C
Cuts at: 4319 4319
Size: 6994
MfeI C'AATT_G
Cuts at: 2948 4593 2948
Size: 1645 5349
MluI A'CGCG_T
Cuts at: 6870 6870
Size: 6994
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2386 6108 6292 1305
Size: 1081 3722 184 2007
Fragments arranged by size:
3722 2007 1081 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2621 2814 10
Size: 54 1425 343 789 193 4190
Fragments arranged by size:
4190 1425 789 343 193 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2567 3113 3732 4952 5311 5470 518
Size: 648 1401 546 619 1220 359 159 2042
Fragments arranged by size:
2042 1401 1220 648 619 546 359 159
MspA1I CmG'CkG
Cuts at: 1606 2332 2965 2980 4093 4410 5087 6235 6480
Size: 726 633 15 1113 317 677 1148 245
Cuts at: 6480 1606
Size: 2120
Fragments arranged by size:
2120 1148 1113 726 677 633 317 245
15
NarI GG'CG_CC
Cuts at: 2793 2793
Size: 6994
NcoI C'CATG_G
Cuts at: 513 1065 2004 2872 3375 3733 513
Size: 552 939 868 503 358 3774
Fragments arranged by size:
3774 939 868 552 503 358
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6994
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6994
NotI GC'GGCC_GC
Cuts at: 4441 4441
Size: 6994
NspI r_CATG'y
Cuts at: 1132 1229 2291 4352 5897 1132
Size: 97 1062 2061 1545 2229
Fragments arranged by size:
2229 2061 1545 1062 97
PasI CC'CwG_GG
Cuts at: 1348 2918 1348
Size: 1570 5424
PciI A'CATG_T
Cuts at: 5893 5893
Size: 6994
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2965 1108
Size: 1857 5137
PmeI GTTT'AAAC
Cuts at: 4298 4298
Size: 6994
PmlI CAC'GTG
Cuts at: 3188 3188
Size: 6994
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5063 5095 6576 6608 5063
Size: 32 1481 32 5449
Fragments arranged by size:
5449 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3244 3380 3244
Size: 136 6858
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6994
PsiI TTA'TAA
Cuts at: 4564 4564
Size: 6994
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2657 2689 4079 4111 2657
Size: 32 1390 32 5540
Fragments arranged by size:
5540 1390 32 32
PstI C_TGCA'G
Cuts at: 830 4346 830
Size: 3516 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6994
PvuII CAG'CTG
Cuts at: 2332 2965 2980 2332
Size: 633 15 6346
Fragments arranged by size:
6346 633 15
SalI G'TCGA_C
Cuts at: 1936 4336 1936
Size: 2400 4594
SapI GCTCTTCn'nnn_
Cuts at: 4239 4239
Size: 6994
SbfI CC_TGCA'GG
Cuts at: 4346 4346
Size: 6994
ScaI AGT'ACT
Cuts at: 2671 5242 6954 2671
Size: 2571 1712 2711
Fragments arranged by size:
2711 2571 1712
SfcI C'TryA_G
Cuts at: 826 1045 2457 4342 5477 6158 6349 826
Size: 219 1412 1885 1135 681 191 1471
Fragments arranged by size:
1885 1471 1412 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2794 2794
Size: 6994
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6994
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6994
SmaI CCC'GGG
Cuts at: 1872 4321 1872
Size: 2449 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3540 4103 5098 5999 6261 820
Size: 197 926 1597 563 995 901 262 1553
Fragments arranged by size:
1597 1553 995 926 901 563 262 197
SnaBI TAC'GTA
Cuts at: 493 4785 493
Size: 4292 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6994
SphI G_CATG'C
Cuts at: 1132 2291 4352 1132
Size: 1159 2061 3774
Fragments arranged by size:
3774 2061 1159
SspI AAT'ATT
Cuts at: 5 52 4918 5
Size: 47 4866 2081
Fragments arranged by size:
4866 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6994
StyI C'CwwG_G
Cuts at: 513 1065 2004 2622 2872 3375 3733 3969 4433
Size: 552 939 618 250 503 358 236 464
Cuts at: 4433 513
Size: 3074
Fragments arranged by size:
3074 939 618 552 503 464 358 250
236
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3342 3421 5021 5038 5191 5376 5821
Size: 1255 1762 79 1600 17 153 185 445
Cuts at: 5821 325
Size: 1498
Fragments arranged by size:
1762 1600 1498 1255 445 185 153 79
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2144 2669 4062
Size: 274 80 33 51 1443 167 525 1393
Cuts at: 4062 5240 6952 96
Size: 1178 1712 138
Fragments arranged by size:
1712 1443 1393 1178 525 274 167 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2134 2166 6954 6986 2134
Size: 32 4788 32 2142
Fragments arranged by size:
4788 2142 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4036 1316
Size: 2720 4274
XbaI T'CTAG_A
Cuts at: 4330 4330
Size: 6994
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6994
XmaI C'CCGG_G
Cuts at: 1870 4319 1870
Size: 2449 4545
XmnI GAAnn'nnTTC
Cuts at: 5123 5123
Size: 6994
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4802 276
Size: 53 83 186 936 3268 2468
Fragments arranged by size:
3268 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PspOMI PspXI RsrII SacI SacII SanDI SexAI SfiI
SrfI SwaI XcmI