Restriction map for
pFN21AE0695
(Circular) (Six-base) MAPSORT of: pFN21AE0695.seq Check: 4964 from: 1 to: 5557
pFN21AE0695 5557 bp
With 182 enzymes: SgfI *
October 20, 2009 10:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3367 278
Size: 53 83 186 936 1831 2468
Fragments arranged by size:
2468 1831 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2900 1937
Size: 963 4594
Acc65I G'GTAC_C
Cuts at: 2017 2878 2017
Size: 861 4696
AclI AA'CG_TT
Cuts at: 1563 3684 4057 1563
Size: 2121 373 3063
Fragments arranged by size:
3063 2121 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2390 2495 3620 5004 5272 1632
Size: 758 105 1125 1384 268 1917
Fragments arranged by size:
1917 1384 1125 758 268 105
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5360
AflIII A'CryG_T
Cuts at: 1203 4456 5433 1203
Size: 3253 977 1327
Fragments arranged by size:
3253 1327 977
AgeI A'CCGG_T
Cuts at: 4388 4388
Size: 5557
AhdI GACnn_n'nnGTC
Cuts at: 4286 4286
Size: 5557
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5557
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5557
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2819 2851 2819
Size: 32 5525
AlwNI CAG_nnn'CTG
Cuts at: 2653 3300 4872 2653
Size: 647 1572 3338
Fragments arranged by size:
3338 1572 647
ApaLI G'TGCA_C
Cuts at: 3614 4770 3614
Size: 1156 4401
ApoI r'AATT_y
Cuts at: 1493 2866 3092 3244 5353 1493
Size: 1373 226 152 2109 1697
Fragments arranged by size:
2109 1697 1373 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4111 160
Size: 872 3079 1606
Fragments arranged by size:
3079 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5557
AvaI C'yCGr_G
Cuts at: 1870 1943 2882 1870
Size: 73 939 4545
Fragments arranged by size:
4545 939 73
BamHI G'GATC_C
Cuts at: 2887 3254 2887
Size: 367 5190
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2017 2291 2452 2878 4333
Size: 325 217 627 230 274 161 426 1455
Cuts at: 4333 618
Size: 1842
Fragments arranged by size:
1842 1455 627 426 325 274 230 217
161
BanII G_rGCy'C
Cuts at: 1391 2075 2233 2876 1391
Size: 684 158 643 4072
Fragments arranged by size:
4072 684 643 158
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2146 2606 2715 928
Size: 971 247 460 109 3770
Fragments arranged by size:
3770 971 460 247 109
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3748 3782 1903
Size: 34 1811 34 3678
Fragments arranged by size:
3678 1811 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3449 4659 3449
Size: 1210 4347
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5557
BfrBI ATG'CAT
Cuts at: 2642 2642
Size: 5557
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2237 4168 136
Size: 107 122 71 631 605 565 1931 1525
Fragments arranged by size:
1931 1525 631 605 565 122 107 71
BglII A'GATC_T
Cuts at: 5552 5552
Size: 5557
BlpI GC'TnA_GC
Cuts at: 2069 2974 2069
Size: 905 4652
Bme1580I G_kGCm'C
Cuts at: 1790 3618 4774 1790
Size: 1828 1156 2573
Fragments arranged by size:
2573 1828 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5557
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2697 3288 4246
Size: 416 53 83 399 24 1280 591 958
Cuts at: 4246 442
Size: 1753
Fragments arranged by size:
1753 1280 958 591 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5557
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2089 2089
Size: 5557
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2256 4217 1239
Size: 1017 1961 2579
Fragments arranged by size:
2579 1961 1017
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3682 4547 4845 3682
Size: 865 298 4394
Fragments arranged by size:
4394 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4220 882
Size: 617 2721 2219
Fragments arranged by size:
2721 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3348 493
Size: 711 2144 2702
Fragments arranged by size:
2702 2144 711
BsaBI GATnn'nnATC
Cuts at: 5551 5551
Size: 5557
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3364 3746 4381 275
Size: 53 83 186 936 1831 382 635 1451
Fragments arranged by size:
1831 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2075 2247 3990 4388 4662 4809 5220 2075
Size: 172 1743 398 274 147 411 2412
Fragments arranged by size:
2412 1743 411 398 274 172 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2786 2816 2819 2849 1178
Size: 30 165 30 1383 30 3 30 3886
Fragments arranged by size:
3886 1383 165 30 30 30 30 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2561 2807 2840 1186
Size: 1375 246 33 3903
Fragments arranged by size:
3903 1375 246 33
BseYI C'CCAG_C
Cuts at: 4760 5256 4760
Size: 496 5061
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4984
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2088 2248 3007 3768 4796 1540
Size: 275 186 87 160 759 761 1028 2301
Fragments arranged by size:
2301 1028 761 759 275 186 160 87
BsiHKAI G_wGCw'C
Cuts at: 2075 3618 3703 4774 2075
Size: 1543 85 1071 2858
Fragments arranged by size:
2858 1543 1071 85
BsmI GAATG_Cn'
Cuts at: 2642 3068 3161 2642
Size: 426 93 5038
Fragments arranged by size:
5038 426 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2075 2233 2876 3618 3703 4774 1391
Size: 399 285 158 643 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 643 399 285 158 85
BspEI T'CCGG_A
Cuts at: 2075 5220 2075
Size: 3145 2412
BspHI T'CATG_A
Cuts at: 3446 5176 3446
Size: 1730 3827
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2912 844
Size: 2068 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3444 1394
Size: 429 1621 3507
Fragments arranged by size:
3507 1621 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4052 4226 65
Size: 1183 2804 174 1396
Fragments arranged by size:
2804 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4201 4388 1700
Size: 110 2391 187 2869
Fragments arranged by size:
2869 2391 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5557
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5557
BssSI C'ACGA_G
Cuts at: 3617 4629 3617
Size: 1012 4545
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2219 794
Size: 1425 4132
BstBI TT'CG_AA
Cuts at: 4361 4361
Size: 5557
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5557
BstYI r'GATC_y
Cuts at: 1972 2887 3254 3639 3656 5108 5194 5206 5552
Size: 915 367 385 17 1452 86 12 346
Cuts at: 5552 1972
Size: 1977
Fragments arranged by size:
1977 1452 915 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2082 513
Size: 552 939 78 3988
Fragments arranged by size:
3988 939 552 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4818
Fragments arranged by size:
4818 683 56
BtsI GCAGTG_nn'
Cuts at: 2175 3073 3867 3887 2175
Size: 898 794 20 3845
Fragments arranged by size:
3845 898 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5557
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5522
DraI TTT'AAA
Cuts at: 2861 2861
Size: 5557
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4564 809
Size: 507 3248 1802
Fragments arranged by size:
3248 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2085 2785 3004
Size: 54 1425 50 275 18 255 700 219
Cuts at: 3004 3893 5522 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 700 275 255 219 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2085 3004 1537
Size: 275 273 919 4090
Fragments arranged by size:
4090 919 275 273
EarI CTCTTCn'nnn_
Cuts at: 1353 2826 3487 1353
Size: 1473 661 3423
Fragments arranged by size:
3423 1473 661
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 4144 4518 4664 387
Size: 1064 138 435 2120 374 146 1280
Fragments arranged by size:
2120 1280 1064 435 374 146 138
EcoICRI GAG'CTC
Cuts at: 2073 2073
Size: 5557
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2256 2390 2495 3620 4217 5004 5272
Size: 393 624 134 105 1125 597 787 268
Cuts at: 5272 1239
Size: 1524
Fragments arranged by size:
1524 1125 787 624 597 393 268 134
105
EcoRI G'AATT_C
Cuts at: 2866 3244 2866
Size: 378 5179
FspI TGC'GCA
Cuts at: 4063 4063
Size: 5557
HaeII r_GCGC'y
Cuts at: 1678 4704 1678
Size: 3026 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4198 4230 4272 4304 1373
Size: 32 135 32 2626 32 42 32 2626
Fragments arranged by size:
2626 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2901 3147 669
Size: 988 281 963 246 3079
Fragments arranged by size:
3079 988 963 281 246
HindIII A'AGCT_T
Cuts at: 748 2402 2612 748
Size: 1654 210 3693
Fragments arranged by size:
3693 1654 210
HpaI GTT'AAC
Cuts at: 3147 3147
Size: 5557
KpnI G_GTAC'C
Cuts at: 2021 2882 2021
Size: 861 4696
MfeI C'AATT_G
Cuts at: 3156 3156
Size: 5557
MluI A'CGCG_T
Cuts at: 5433 5433
Size: 5557
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4671 4855 1305
Size: 3366 184 2007
Fragments arranged by size:
3366 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2787 10
Size: 54 1425 343 955 2780
Fragments arranged by size:
2780 1425 955 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3515 3874 4033 518
Size: 648 2349 359 159 2042
Fragments arranged by size:
2349 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2973 3650 4798 5043 1606
Size: 1367 677 1148 245 2120
Fragments arranged by size:
2120 1367 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4066
Fragments arranged by size:
4066 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5557
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5557
NotI GC'GGCC_GC
Cuts at: 3004 3004
Size: 5557
NsiI A_TGCA'T
Cuts at: 2644 2644
Size: 5557
NspI r_CATG'y
Cuts at: 1132 1229 2244 2915 4460 1132
Size: 97 1015 671 1545 2229
Fragments arranged by size:
2229 1545 1015 671 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5557
PciI A'CATG_T
Cuts at: 4456 4456
Size: 5557
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2353 1108
Size: 1245 4312
PmeI GTTT'AAAC
Cuts at: 2861 2861
Size: 5557
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2819 2851 3626 3658 5139 5171 2819
Size: 32 775 32 1481 32 3205
Fragments arranged by size:
3205 1481 775 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5557
PsiI TTA'TAA
Cuts at: 3127 3127
Size: 5557
PstI C_TGCA'G
Cuts at: 830 2170 2909 830
Size: 1340 739 3478
Fragments arranged by size:
3478 1340 739
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5557
SacI G_AGCT'C
Cuts at: 2075 2075
Size: 5557
SalI G'TCGA_C
Cuts at: 1936 2899 1936
Size: 963 4594
SbfI CC_TGCA'GG
Cuts at: 2909 2909
Size: 5557
ScaI AGT'ACT
Cuts at: 3805 5517 3805
Size: 1712 3845
SfcI C'TryA_G
Cuts at: 826 1045 2166 2905 4040 4721 4912 826
Size: 219 1121 739 1135 681 191 1471
Fragments arranged by size:
1471 1135 1121 739 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5557
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5557
SmaI CCC'GGG
Cuts at: 1872 2884 1872
Size: 1012 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3661 4562 4824 820
Size: 197 926 1718 901 262 1553
Fragments arranged by size:
1718 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3348 493
Size: 2855 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5557
SphI G_CATG'C
Cuts at: 1132 2244 2915 1132
Size: 1112 671 3774
Fragments arranged by size:
3774 1112 671
SspI AAT'ATT
Cuts at: 5 52 3481 5
Size: 47 3429 2081
Fragments arranged by size:
3429 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5557
StyI C'CwwG_G
Cuts at: 513 1065 2004 2996 513
Size: 552 939 992 3074
Fragments arranged by size:
3074 992 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2370 3584 3601 3754 3939 4384 325
Size: 1255 790 1214 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1214 790 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2694 3803 5515
Size: 274 80 33 51 1443 717 1109 1712
Cuts at: 5515 96
Size: 138
Fragments arranged by size:
1712 1443 1109 717 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5517 5549 5517
Size: 32 5525
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5557
XbaI T'CTAG_A
Cuts at: 2668 2893 2668
Size: 225 5332
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5557
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5557
XmaI C'CCGG_G
Cuts at: 1870 2882 1870
Size: 1012 4545
XmnI GAAnn'nnTTC
Cuts at: 2480 3686 2480
Size: 1206 4351
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3365 276
Size: 53 83 186 936 1831 2468
Fragments arranged by size:
2468 1831 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI