Restriction map for
pFN21AE0694
(Circular) (Six-base) MAPSORT of: pFN21AE0694.seq Check: 851 from: 1 to: 5914
pFN21AE0694 5914 bp
With 182 enzymes: SgfI *
October 20, 2009 10:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3724 278
Size: 53 83 186 936 2188 2468
Fragments arranged by size:
2468 2188 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2768 3257 1937
Size: 831 489 4594
Fragments arranged by size:
4594 831 489
Acc65I G'GTAC_C
Cuts at: 3235 3235
Size: 5914
AclI AA'CG_TT
Cuts at: 1563 4041 4414 1563
Size: 2478 373 3063
Fragments arranged by size:
3063 2478 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2391 2886 3977 5361 5629 1632
Size: 759 495 1091 1384 268 1917
Fragments arranged by size:
1917 1384 1091 759 495 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5717
AflIII A'CryG_T
Cuts at: 1203 4813 5790 1203
Size: 3610 977 1327
Fragments arranged by size:
3610 1327 977
AgeI A'CCGG_T
Cuts at: 4745 4745
Size: 5914
AhdI GACnn_n'nnGTC
Cuts at: 4643 4643
Size: 5914
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5914
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2439 1184
Size: 1255 4659
AlwNI CAG_nnn'CTG
Cuts at: 3657 5229 3657
Size: 1572 4342
ApaI G_GGCC'C
Cuts at: 2087 2087
Size: 5914
ApaLI G'TGCA_C
Cuts at: 3971 5127 3971
Size: 1156 4758
ApoI r'AATT_y
Cuts at: 1493 2052 2263 2874 3000 3223 3449 3601 5710
Size: 559 211 611 126 223 226 152 2109
Cuts at: 5710 1493
Size: 1697
Fragments arranged by size:
2109 1697 611 559 226 223 211 152
126
AseI AT'TA_AT
Cuts at: 160 1032 4468 160
Size: 872 3436 1606
Fragments arranged by size:
3436 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5914
AvaI C'yCGr_G
Cuts at: 1870 1943 3239 1870
Size: 73 1296 4545
Fragments arranged by size:
4545 1296 73
BamHI G'GATC_C
Cuts at: 3244 3611 3244
Size: 367 5547
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2252 3235 4690 618
Size: 325 217 627 465 983 1455 1842
Fragments arranged by size:
1842 1455 983 627 465 325 217
BanII G_rGCy'C
Cuts at: 1391 2087 3233 1391
Size: 696 1146 4072
Fragments arranged by size:
4072 1146 696
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2634 928
Size: 971 735 4208
Fragments arranged by size:
4208 971 735
BbvCI CC'TCA_GC
Cuts at: 2673 2692 2673
Size: 19 5895
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4105 4139 1903
Size: 34 2168 34 3678
Fragments arranged by size:
3678 2168 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3806 5016 3806
Size: 1210 4704
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5914
BfrBI ATG'CAT
Cuts at: 2420 2420
Size: 5914
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2961 4525 136
Size: 107 122 71 631 605 1289 1564 1525
Fragments arranged by size:
1564 1525 1289 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3208 5909 3208
Size: 2701 3213
BlpI GC'TnA_GC
Cuts at: 3331 3331
Size: 5914
Bme1580I G_kGCm'C
Cuts at: 1790 2087 2160 3975 5131 1790
Size: 297 73 1815 1156 2573
Fragments arranged by size:
2573 1815 1156 297 73
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5914
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2343 2812 3645
Size: 416 53 83 399 24 926 469 833
Cuts at: 3645 4603 442
Size: 958 1753
Fragments arranged by size:
1753 958 926 833 469 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2955 1056
Size: 1899 4015
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4574 1239
Size: 3335 2579
Bpu10I CC'TnA_GC
Cuts at: 2673 2692 2673
Size: 19 5895
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4039 4904 5202 4039
Size: 865 298 4751
Fragments arranged by size:
4751 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2036 4577 882
Size: 617 537 2541 2219
Fragments arranged by size:
2541 2219 617 537
BsaAI yAC'GTr
Cuts at: 493 1204 3705 493
Size: 711 2501 2702
Fragments arranged by size:
2702 2501 711
BsaBI GATnn'nnATC
Cuts at: 5908 5908
Size: 5914
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 3721 4103 4738
Size: 53 83 186 936 476 1712 382 635
Cuts at: 4738 275
Size: 1451
Fragments arranged by size:
1712 1451 936 635 476 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4347 4745 5019 5166 5577 4347
Size: 398 274 147 411 4684
Fragments arranged by size:
4684 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3084 3114 1178
Size: 30 165 30 1681 30 3978
Fragments arranged by size:
3978 1681 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5914
BseYI C'CCAG_C
Cuts at: 2013 2311 5117 5613 2013
Size: 298 2806 496 2314
Fragments arranged by size:
2806 2314 496 298
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5341
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3364 4125 5153 1540
Size: 275 186 1363 761 1028 2301
Fragments arranged by size:
2301 1363 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3975 4060 5131 3975
Size: 85 1071 4758
Fragments arranged by size:
4758 1071 85
BsmI GAATG_Cn'
Cuts at: 3425 3518 3425
Size: 93 5821
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2087 2160 3233 3975 4060 5131 1391
Size: 399 297 73 1073 742 85 1071 2174
Fragments arranged by size:
2174 1073 1071 742 399 297 85 73
BspEI T'CCGG_A
Cuts at: 5577 5577
Size: 5914
BspHI T'CATG_A
Cuts at: 3803 5533 3803
Size: 1730 4184
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2230 2992 3269 844
Size: 1386 762 277 3489
Fragments arranged by size:
3489 1386 762 277
BsrBI CCG'CTC
Cuts at: 1394 1823 3801 1394
Size: 429 1978 3507
Fragments arranged by size:
3507 1978 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4409 4583 65
Size: 1183 3161 174 1396
Fragments arranged by size:
3161 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4558 4745 1700
Size: 110 2748 187 2869
Fragments arranged by size:
2869 2748 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5914
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5914
BssSI C'ACGA_G
Cuts at: 3974 4986 3974
Size: 1012 4902
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5914
BstBI TT'CG_AA
Cuts at: 4718 4718
Size: 5914
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5914
BstYI r'GATC_y
Cuts at: 1972 3208 3244 3611 3996 4013 5465 5551 5563
Size: 1236 36 367 385 17 1452 86 12
Cuts at: 5563 5909 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1236 385 367 346 86 36
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3096 513
Size: 552 939 1092 3331
Fragments arranged by size:
3331 1092 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5175
Fragments arranged by size:
5175 683 56
BtsI GCAGTG_nn'
Cuts at: 2209 3430 4224 4244 2209
Size: 1221 794 20 3879
Fragments arranged by size:
3879 1221 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5914
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2917 2952 529
Size: 35 2353 35 3491
Fragments arranged by size:
3491 2353 35 35
DraI TTT'AAA
Cuts at: 3218 3218
Size: 5914
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4921 809
Size: 507 3605 1802
Fragments arranged by size:
3605 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2705 2962 3361
Size: 54 1425 50 275 18 875 257 399
Cuts at: 3361 4250 5879 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 875 399 275 257 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3361 1537
Size: 275 1549 4090
Fragments arranged by size:
4090 1549 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2605 3844 1353
Size: 1252 1239 3423
Fragments arranged by size:
3423 1252 1239
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4501 4875 5021 387
Size: 1064 138 2912 374 146 1280
Fragments arranged by size:
2912 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2391 2886 3977 4574 5361 5629 1239
Size: 393 759 495 1091 597 787 268 1524
Fragments arranged by size:
1524 1091 787 759 597 495 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2084 2111 2084
Size: 27 5887
EcoRI G'AATT_C
Cuts at: 2052 3223 3601 2052
Size: 1171 378 4365
Fragments arranged by size:
4365 1171 378
FspI TGC'GCA
Cuts at: 4420 4420
Size: 5914
HaeII r_GCGC'y
Cuts at: 1678 5061 1678
Size: 3383 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4555 4587 4629 4661 1373
Size: 32 135 32 2983 32 42 32 2626
Fragments arranged by size:
2983 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3258 3504 669
Size: 988 281 1320 246 3079
Fragments arranged by size:
3079 1320 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5914
HpaI GTT'AAC
Cuts at: 3504 3504
Size: 5914
KpnI G_GTAC'C
Cuts at: 3239 3239
Size: 5914
MfeI C'AATT_G
Cuts at: 3513 3513
Size: 5914
MluI A'CGCG_T
Cuts at: 5790 5790
Size: 5914
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5028 5212 1305
Size: 3723 184 2007
Fragments arranged by size:
3723 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2707 2964 10
Size: 54 1425 343 875 257 2960
Fragments arranged by size:
2960 1425 875 343 257 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2778 3872 4231 4390 518
Size: 648 1612 1094 359 159 2042
Fragments arranged by size:
2042 1612 1094 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2462 2593 3330 4007 5155 5400 1606
Size: 856 131 737 677 1148 245 2120
Fragments arranged by size:
2120 1148 856 737 677 245 131
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4423
Fragments arranged by size:
4423 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5914
NheI G'CTAG_C
Cuts at: 1052 2951 1052
Size: 1899 4015
NotI GC'GGCC_GC
Cuts at: 3361 3361
Size: 5914
NsiI A_TGCA'T
Cuts at: 2422 2422
Size: 5914
NspI r_CATG'y
Cuts at: 1132 1229 2420 2664 3272 4817 1132
Size: 97 1191 244 608 1545 2229
Fragments arranged by size:
2229 1545 1191 608 244 97
PasI CC'CwG_GG
Cuts at: 1348 2037 1348
Size: 689 5225
PciI A'CATG_T
Cuts at: 4813 4813
Size: 5914
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2037 1108
Size: 929 4985
PfoI T'CCnGG_A
Cuts at: 2056 2056
Size: 5914
PmeI GTTT'AAAC
Cuts at: 3218 3218
Size: 5914
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3983 4015 5496 5528 3983
Size: 32 1481 32 4369
Fragments arranged by size:
4369 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2111 2111
Size: 5914
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5914
PsiI TTA'TAA
Cuts at: 3484 3484
Size: 5914
PspOMI G'GGCC_C
Cuts at: 2083 2083
Size: 5914
PstI C_TGCA'G
Cuts at: 830 3266 830
Size: 2436 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5914
PvuII CAG'CTG
Cuts at: 2462 2462
Size: 5914
RsrII CG'GwC_CG
Cuts at: 2066 2066
Size: 5914
SalI G'TCGA_C
Cuts at: 1936 3256 1936
Size: 1320 4594
SbfI CC_TGCA'GG
Cuts at: 3266 3266
Size: 5914
ScaI AGT'ACT
Cuts at: 4162 5874 4162
Size: 1712 4202
SfcI C'TryA_G
Cuts at: 826 1045 2281 2588 3262 4397 5078 5269 826
Size: 219 1236 307 674 1135 681 191 1471
Fragments arranged by size:
1471 1236 1135 681 674 307 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5914
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5914
SmaI CCC'GGG
Cuts at: 1872 3241 1872
Size: 1369 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4018 4919 5181 820
Size: 197 926 2075 901 262 1553
Fragments arranged by size:
2075 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3705 493
Size: 3212 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5914
SphI G_CATG'C
Cuts at: 1132 2420 2664 3272 1132
Size: 1288 244 608 3774
Fragments arranged by size:
3774 1288 608 244
SspI AAT'ATT
Cuts at: 5 52 2712 3838 5
Size: 47 2660 1126 2081
Fragments arranged by size:
2660 2081 1126 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5914
StyI C'CwwG_G
Cuts at: 513 1065 2004 2174 2665 2734 3353 513
Size: 552 939 170 491 69 619 3074
Fragments arranged by size:
3074 939 619 552 491 170 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2362 2694 3941 3958 4111 4296 4741
Size: 1255 782 332 1247 17 153 185 445
Cuts at: 4741 325
Size: 1498
Fragments arranged by size:
1498 1255 1247 782 445 332 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4160 5872 96
Size: 274 80 33 51 1443 2183 1712 138
Fragments arranged by size:
2183 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5874 5906 5874
Size: 32 5882
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5914
XbaI T'CTAG_A
Cuts at: 3250 3250
Size: 5914
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5914
XmaI C'CCGG_G
Cuts at: 1870 3239 1870
Size: 1369 4545
XmnI GAAnn'nnTTC
Cuts at: 4043 4043
Size: 5914
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3722 276
Size: 53 83 186 936 2188 2468
Fragments arranged by size:
2468 2188 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII RsrII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PspXI PsrI SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI