Restriction map for
pFN21AE0692
(Circular) (Six-base) MAPSORT of: pFN21AE0692.seq Check: 2381 from: 1 to: 5911
pFN21AE0692 5911 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2285 3721 278
Size: 53 83 186 936 749 1436 2468
Fragments arranged by size:
2468 1436 936 749 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2601 3254 1937
Size: 664 653 4594
Fragments arranged by size:
4594 664 653
Acc65I G'GTAC_C
Cuts at: 3232 3232
Size: 5911
AclI AA'CG_TT
Cuts at: 1563 4038 4411 1563
Size: 2475 373 3063
Fragments arranged by size:
3063 2475 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2679 3150 3974 5358 5626 1632
Size: 1047 471 824 1384 268 1917
Fragments arranged by size:
1917 1384 1047 824 471 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5714
AflIII A'CryG_T
Cuts at: 1203 4810 5787 1203
Size: 3607 977 1327
Fragments arranged by size:
3607 1327 977
AgeI A'CCGG_T
Cuts at: 4742 4742
Size: 5911
AhdI GACnn_n'nnGTC
Cuts at: 4640 4640
Size: 5911
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5911
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5911
AlwNI CAG_nnn'CTG
Cuts at: 3654 5226 3654
Size: 1572 4339
ApaLI G'TGCA_C
Cuts at: 2452 3968 5124 2452
Size: 1516 1156 3239
Fragments arranged by size:
3239 1516 1156
ApoI r'AATT_y
Cuts at: 1493 2888 2982 3220 3446 3598 5707 1493
Size: 1395 94 238 226 152 2109 1697
Fragments arranged by size:
2109 1697 1395 238 226 152 94
AseI AT'TA_AT
Cuts at: 160 1032 4465 160
Size: 872 3433 1606
Fragments arranged by size:
3433 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5911
AvaI C'yCGr_G
Cuts at: 1870 1943 3236 1870
Size: 73 1293 4545
Fragments arranged by size:
4545 1293 73
BamHI G'GATC_C
Cuts at: 3241 3608 3241
Size: 367 5544
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2066 2467 2621 3232 4687
Size: 325 217 627 279 401 154 611 1455
Cuts at: 4687 618
Size: 1842
Fragments arranged by size:
1842 1455 627 611 401 325 279 217
154
BanII G_rGCy'C
Cuts at: 1391 3230 1391
Size: 1839 4072
BbeI G_GCGC'C
Cuts at: 2070 2070
Size: 5911
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2211 2970 928
Size: 971 312 759 3869
Fragments arranged by size:
3869 971 759 312
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4102 4136 1903
Size: 34 2165 34 3678
Fragments arranged by size:
3678 2165 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3803 5013 3803
Size: 1210 4701
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5911
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4522 136
Size: 107 122 71 631 605 2850 1525
Fragments arranged by size:
2850 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2698 3114 5906 2698
Size: 416 2792 2703
Fragments arranged by size:
2792 2703 416
BlpI GC'TnA_GC
Cuts at: 2403 3328 2403
Size: 925 4986
Bme1580I G_kGCm'C
Cuts at: 1790 2456 3972 5128 1790
Size: 666 1516 1156 2573
Fragments arranged by size:
2573 1516 1156 666
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5911
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3642 4600 442
Size: 416 53 83 399 24 2225 958 1753
Fragments arranged by size:
2225 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5911
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2102 2236 2253 4571 1239
Size: 863 134 17 2318 2579
Fragments arranged by size:
2579 2318 863 134 17
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4036 4901 5199 4036
Size: 865 298 4748
Fragments arranged by size:
4748 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2600 4574 882
Size: 617 1101 1974 2219
Fragments arranged by size:
2219 1974 1101 617
BsaAI yAC'GTr
Cuts at: 493 1204 3702 493
Size: 711 2498 2702
Fragments arranged by size:
2702 2498 711
BsaBI GATnn'nnATC
Cuts at: 2438 5905 2438
Size: 3467 2444
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2067 2282 3718 4100
Size: 53 83 186 936 534 215 1436 382
Cuts at: 4100 4735 275
Size: 635 1451
Fragments arranged by size:
1451 1436 936 635 534 382 215 186
83 53
BsaWI w'CCGG_w
Cuts at: 4344 4742 5016 5163 5574 4344
Size: 398 274 147 411 4681
Fragments arranged by size:
4681 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2155 2185 2504 2534 1178
Size: 30 165 30 752 30 319 30 4555
Fragments arranged by size:
4555 752 319 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5911
BseYI C'CCAG_C
Cuts at: 2246 2472 5114 5610 2246
Size: 226 2642 496 2547
Fragments arranged by size:
2642 2547 496 226
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5338
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3361 4122 5150 1540
Size: 275 186 1360 761 1028 2301
Fragments arranged by size:
2301 1360 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2456 3972 4057 5128 2456
Size: 1516 85 1071 3239
Fragments arranged by size:
3239 1516 1071 85
BsmI GAATG_Cn'
Cuts at: 3422 3515 3422
Size: 93 5818
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2456 3230 3972 4057 5128 1391
Size: 399 666 774 742 85 1071 2174
Fragments arranged by size:
2174 1071 774 742 666 399 85
BspEI T'CCGG_A
Cuts at: 5574 5574
Size: 5911
BspHI T'CATG_A
Cuts at: 2335 2536 3800 5530 2335
Size: 201 1264 1730 2716
Fragments arranged by size:
2716 1730 1264 201
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3266 844
Size: 2422 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3798 1394
Size: 429 1975 3507
Fragments arranged by size:
3507 1975 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2571 4406 4580 65
Size: 1183 1323 1835 174 1396
Fragments arranged by size:
1835 1396 1323 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4555 4742 1700
Size: 110 2745 187 2869
Fragments arranged by size:
2869 2745 187 110
BsrGI T'GTAC_A
Cuts at: 96 2883 96
Size: 2787 3124
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5911
BssSI C'ACGA_G
Cuts at: 3971 4983 3971
Size: 1012 4899
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2413 794
Size: 1619 4292
BstBI TT'CG_AA
Cuts at: 4715 4715
Size: 5911
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5911
BstYI r'GATC_y
Cuts at: 1972 2698 2809 2837 3114 3241 3608 3993 4010
Size: 726 111 28 277 127 367 385 17
Cuts at: 4010 5462 5548 5560 5906 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 726 385 367 346 277 127
111 86 28 17 12
Bsu36I CC'TnA_GG
Cuts at: 2715 2750 2715
Size: 35 5876
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5359
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5172
Fragments arranged by size:
5172 683 56
BtsI GCAGTG_nn'
Cuts at: 2668 3427 4221 4241 2668
Size: 759 794 20 4338
Fragments arranged by size:
4338 794 759 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5911
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5876
DraI TTT'AAA
Cuts at: 3215 3215
Size: 5911
DraIII CAC_nnn'GTG
Cuts at: 2230 2230
Size: 5911
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4918 809
Size: 507 3602 1802
Fragments arranged by size:
3602 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3358 4247 5876
Size: 54 1425 50 275 18 1528 889 1629
Cuts at: 5876 8
Size: 43
Fragments arranged by size:
1629 1528 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3358 1537
Size: 275 1546 4090
Fragments arranged by size:
4090 1546 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3841 1353
Size: 2488 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4498 4872 5018 387
Size: 1064 138 2909 374 146 1280
Fragments arranged by size:
2909 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2102 2236 2253 2679 3150 3974 4571
Size: 393 470 134 17 426 471 824 597
Cuts at: 4571 5358 5626 1239
Size: 787 268 1524
Fragments arranged by size:
1524 824 787 597 471 470 426 393
268 134 17
EcoNI CCTnn'n_nnAGG
Cuts at: 2268 2268
Size: 5911
EcoRI G'AATT_C
Cuts at: 3220 3598 3220
Size: 378 5533
FspI TGC'GCA
Cuts at: 4417 4417
Size: 5911
HaeII r_GCGC'y
Cuts at: 1678 2070 5058 1678
Size: 392 2988 2531
Fragments arranged by size:
2988 2531 392
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4552 4584 4626 4658 1373
Size: 32 135 32 2980 32 42 32 2626
Fragments arranged by size:
2980 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3255 3501 669
Size: 988 281 1317 246 3079
Fragments arranged by size:
3079 1317 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5911
HpaI GTT'AAC
Cuts at: 3501 3501
Size: 5911
KasI G'GCGC_C
Cuts at: 2066 2066
Size: 5911
KpnI G_GTAC'C
Cuts at: 3236 3236
Size: 5911
MfeI C'AATT_G
Cuts at: 3510 3510
Size: 5911
MluI A'CGCG_T
Cuts at: 5787 5787
Size: 5911
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5025 5209 1305
Size: 3720 184 2007
Fragments arranged by size:
3720 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4089
Fragments arranged by size:
4089 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2313 2387 3869 4228 4387 518
Size: 648 1147 74 1482 359 159 2042
Fragments arranged by size:
2042 1482 1147 648 359 159 74
MspA1I CmG'CkG
Cuts at: 1606 2476 3327 4004 5152 5397 1606
Size: 870 851 677 1148 245 2120
Fragments arranged by size:
2120 1148 870 851 677 245
NarI GG'CG_CC
Cuts at: 2067 2067
Size: 5911
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5359
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5911
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5911
NotI GC'GGCC_GC
Cuts at: 3358 3358
Size: 5911
NspI r_CATG'y
Cuts at: 1132 1229 3269 4814 1132
Size: 97 2040 1545 2229
Fragments arranged by size:
2229 2040 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5911
PciI A'CATG_T
Cuts at: 4810 4810
Size: 5911
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2789 1108
Size: 1681 4230
PfoI T'CCnGG_A
Cuts at: 2269 2269
Size: 5911
PmeI GTTT'AAAC
Cuts at: 3215 3215
Size: 5911
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3980 4012 5493 5525 3980
Size: 32 1481 32 4366
Fragments arranged by size:
4366 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5911
PsiI TTA'TAA
Cuts at: 3481 3481
Size: 5911
PstI C_TGCA'G
Cuts at: 830 3263 830
Size: 2433 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5911
SalI G'TCGA_C
Cuts at: 1936 3253 1936
Size: 1317 4594
SbfI CC_TGCA'GG
Cuts at: 3263 3263
Size: 5911
ScaI AGT'ACT
Cuts at: 4159 5871 4159
Size: 1712 4199
SfcI C'TryA_G
Cuts at: 826 1045 2515 3259 4394 5075 5266 826
Size: 219 1470 744 1135 681 191 1471
Fragments arranged by size:
1471 1470 1135 744 681 219 191
SfoI GGC'GCC
Cuts at: 2068 2068
Size: 5911
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5911
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5911
SmaI CCC'GGG
Cuts at: 1872 3238 1872
Size: 1366 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4015 4916 5178 820
Size: 197 926 2072 901 262 1553
Fragments arranged by size:
2072 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3702 493
Size: 3209 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5911
SphI G_CATG'C
Cuts at: 1132 3269 1132
Size: 2137 3774
SspI AAT'ATT
Cuts at: 5 52 3835 5
Size: 47 3783 2081
Fragments arranged by size:
3783 2081 47
StuI AGG'CCT
Cuts at: 1517 3180 1517
Size: 1663 4248
StyI C'CwwG_G
Cuts at: 513 1065 2052 3350 513
Size: 552 987 1298 3074
Fragments arranged by size:
3074 1298 987 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3063 3938 3955 4108 4293 4738 325
Size: 1255 1483 875 17 153 185 445 1498
Fragments arranged by size:
1498 1483 1255 875 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2883 3196 4157
Size: 274 80 33 51 1443 906 313 961
Cuts at: 4157 5869 96
Size: 1712 138
Fragments arranged by size:
1712 1443 961 906 313 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2155 2187 5871 5903 2155
Size: 32 3684 32 2163
Fragments arranged by size:
3684 2163 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2597 1316
Size: 1281 4630
XbaI T'CTAG_A
Cuts at: 3247 3247
Size: 5911
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2059 2059
Size: 5911
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5911
XmaI C'CCGG_G
Cuts at: 1870 3236 1870
Size: 1366 4545
XmnI GAAnn'nnTTC
Cuts at: 4040 4040
Size: 5911
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2283 3719 276
Size: 53 83 186 936 749 1436 2468
Fragments arranged by size:
2468 1436 936 749 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI
EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SrfI
SwaI