Restriction map for
pFN21AE0690
(Circular) (Six-base) MAPSORT of: pFN21AE0690.seq Check: 7213 from: 1 to: 5908
pFN21AE0690 5908 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3718 278
Size: 53 83 186 936 2182 2468
Fragments arranged by size:
2468 2182 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3251 1937
Size: 1314 4594
Acc65I G'GTAC_C
Cuts at: 3229 3229
Size: 5908
AclI AA'CG_TT
Cuts at: 1563 4035 4408 1563
Size: 2472 373 3063
Fragments arranged by size:
3063 2472 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2708 2898 3971 5355 5623 1632
Size: 1076 190 1073 1384 268 1917
Fragments arranged by size:
1917 1384 1076 1073 268 190
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5711
AflIII A'CryG_T
Cuts at: 1203 3197 4807 5784 1203
Size: 1994 1610 977 1327
Fragments arranged by size:
1994 1610 1327 977
AgeI A'CCGG_T
Cuts at: 4739 4739
Size: 5908
AhdI GACnn_n'nnGTC
Cuts at: 4637 4637
Size: 5908
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5908
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5908
AlwNI CAG_nnn'CTG
Cuts at: 3651 5223 3651
Size: 1572 4336
ApaI G_GGCC'C
Cuts at: 2284 2356 2284
Size: 72 5836
ApaLI G'TGCA_C
Cuts at: 2675 3965 5121 2675
Size: 1290 1156 3462
Fragments arranged by size:
3462 1290 1156
ApoI r'AATT_y
Cuts at: 1493 2345 3217 3443 3595 5704 1493
Size: 852 872 226 152 2109 1697
Fragments arranged by size:
2109 1697 872 852 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4462 160
Size: 872 3430 1606
Fragments arranged by size:
3430 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5908
AvaI C'yCGr_G
Cuts at: 1870 1943 2115 2146 2283 2318 2784 3233 1870
Size: 73 172 31 137 35 466 449 4545
Fragments arranged by size:
4545 466 449 172 137 73 35 31
BamHI G'GATC_C
Cuts at: 2125 2505 3238 3605 2125
Size: 380 733 367 4428
Fragments arranged by size:
4428 733 380 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2024 2080 2373 2723 2779
Size: 325 217 627 237 56 293 350 56
Cuts at: 2779 3229 4684 618
Size: 450 1455 1842
Fragments arranged by size:
1842 1455 627 450 350 325 293 237
217 56 56
BanII G_rGCy'C
Cuts at: 1391 2284 2356 2459 2529 2991 3227 1391
Size: 893 72 103 70 462 236 4072
Fragments arranged by size:
4072 893 462 236 103 72 70
BbeI G_GCGC'C
Cuts at: 2028 2084 2377 2028
Size: 56 293 5559
Fragments arranged by size:
5559 293 56
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4937
BbvCI CC'TCA_GC
Cuts at: 2096 2520 2845 2096
Size: 424 325 5159
Fragments arranged by size:
5159 424 325
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4099 4133 1903
Size: 34 2162 34 3678
Fragments arranged by size:
3678 2162 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3800 5010 3800
Size: 1210 4698
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5908
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2162 2288 4519
Size: 107 122 71 631 605 490 126 2231
Cuts at: 4519 136
Size: 1525
Fragments arranged by size:
2231 1525 631 605 490 126 122 107
71
BglII A'GATC_T
Cuts at: 5903 5903
Size: 5908
BlpI GC'TnA_GC
Cuts at: 3325 3325
Size: 5908
Bme1580I G_kGCm'C
Cuts at: 1790 2284 2356 2679 3969 5125 1790
Size: 494 72 323 1290 1156 2573
Fragments arranged by size:
2573 1290 1156 494 323 72
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5908
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2063 3639 4597
Size: 416 53 83 399 24 646 1576 958
Cuts at: 4597 442
Size: 1753
Fragments arranged by size:
1753 1576 958 646 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5908
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4568 1239
Size: 3329 2579
Bpu10I CC'TnA_GC
Cuts at: 2096 2520 2845 2096
Size: 424 325 5159
Fragments arranged by size:
5159 424 325
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2351 4033 4898 5196 2351
Size: 1682 865 298 3063
Fragments arranged by size:
3063 1682 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2477 2562 2969 4571 882
Size: 617 978 85 407 1602 2219
Fragments arranged by size:
2219 1602 978 617 407 85
BsaAI yAC'GTr
Cuts at: 493 1204 3699 493
Size: 711 2495 2702
Fragments arranged by size:
2702 2495 711
BsaBI GATnn'nnATC
Cuts at: 5902 5902
Size: 5908
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2025 2081 2313 2374
Size: 53 83 186 936 492 56 232 61
Cuts at: 2374 3715 4097 4732 275
Size: 1341 382 635 1451
Fragments arranged by size:
1451 1341 936 635 492 382 232 186
83 61 56 53
BsaWI w'CCGG_w
Cuts at: 2128 4341 4739 5013 5160 5571 2128
Size: 2213 398 274 147 411 2465
Fragments arranged by size:
2465 2213 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5683
Fragments arranged by size:
5683 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2414 2537 1186
Size: 1228 123 4557
Fragments arranged by size:
4557 1228 123
BseYI C'CCAG_C
Cuts at: 2084 2430 2605 2771 5111 5607 2084
Size: 346 175 166 2340 496 2385
Fragments arranged by size:
2385 2340 496 346 175 166
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2411 2642 1163
Size: 573 675 231 4429
Fragments arranged by size:
4429 675 573 231
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2139 2204 3358 4119 5147 1540
Size: 275 186 138 65 1154 761 1028 2301
Fragments arranged by size:
2301 1154 1028 761 275 186 138 65
BsiHKAI G_wGCw'C
Cuts at: 2459 2679 2991 3969 4054 5125 2459
Size: 220 312 978 85 1071 3242
Fragments arranged by size:
3242 1071 978 312 220 85
BsmI GAATG_Cn'
Cuts at: 3419 3512 3419
Size: 93 5815
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2284 2356 2459 2529 2679 2991 3227
Size: 399 494 72 103 70 150 312 236
Cuts at: 3227 3969 4054 5125 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 494 399 312 236 150
103 85 72 70
BspEI T'CCGG_A
Cuts at: 5571 5571
Size: 5908
BspHI T'CATG_A
Cuts at: 3797 5527 3797
Size: 1730 4178
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2478 3263 844
Size: 1634 785 3489
Fragments arranged by size:
3489 1634 785
BsrBI CCG'CTC
Cuts at: 1394 1823 2053 2716 3795 1394
Size: 429 230 663 1079 3507
Fragments arranged by size:
3507 1079 663 429 230
BsrDI GCAATG_nn'
Cuts at: 65 1248 2425 2646 2949 4403 4577 65
Size: 1183 1177 221 303 1454 174 1396
Fragments arranged by size:
1454 1396 1183 1177 303 221 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2101 3153 4552 4739 1700
Size: 110 291 1052 1399 187 2869
Fragments arranged by size:
2869 1399 1052 291 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5908
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5908
BssSI C'ACGA_G
Cuts at: 3968 4980 3968
Size: 1012 4896
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5908
BstBI TT'CG_AA
Cuts at: 4712 4712
Size: 5908
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5908
BstYI r'GATC_y
Cuts at: 1972 2125 2301 2505 3238 3605 3990 4007 5459
Size: 153 176 204 733 367 385 17 1452
Cuts at: 5459 5545 5557 5903 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 733 385 367 346 204 176
153 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2178 2178
Size: 5908
BtgI C'CryG_G
Cuts at: 513 1065 2004 2075 2121 513
Size: 552 939 71 46 4300
Fragments arranged by size:
4300 939 552 71 46
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5169
Fragments arranged by size:
5169 683 56
BtsI GCAGTG_nn'
Cuts at: 2268 3424 4218 4238 2268
Size: 1156 794 20 3938
Fragments arranged by size:
3938 1156 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5908
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5873
DraI TTT'AAA
Cuts at: 3212 3212
Size: 5908
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3097 4915 809
Size: 507 1781 1818 1802
Fragments arranged by size:
1818 1802 1781 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2136 2201 2265
Size: 54 1425 50 275 18 306 65 64
Cuts at: 2265 2742 3355 4244 5873 8
Size: 477 613 889 1629 43
Fragments arranged by size:
1629 1425 889 613 477 306 275 65
64 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2136 2201 3355 1537
Size: 275 324 65 1154 4090
Fragments arranged by size:
4090 1154 324 275 65
EarI CTCTTCn'nnn_
Cuts at: 1353 3838 1353
Size: 2485 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2237 2924 4495 4869 5015 387
Size: 1064 138 648 687 1571 374 146 1280
Fragments arranged by size:
1571 1280 1064 687 648 374 146 138
EcoICRI GAG'CTC
Cuts at: 2457 2989 2457
Size: 532 5376
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2708 2898 3971 4568 5355 5623 1239
Size: 393 1076 190 1073 597 787 268 1524
Fragments arranged by size:
1524 1076 1073 787 597 393 268 190
EcoO109I rG'GnC_Cy
Cuts at: 2143 2280 2353 2683 2997 2143
Size: 137 73 330 314 5054
Fragments arranged by size:
5054 330 314 137 73
EcoRI G'AATT_C
Cuts at: 2345 3217 3595 2345
Size: 872 378 4658
Fragments arranged by size:
4658 872 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2976 3136 2976
Size: 160 5748
FspI TGC'GCA
Cuts at: 4414 4414
Size: 5908
HaeII r_GCGC'y
Cuts at: 1678 2028 2084 2339 2377 2646 3044 5055 1678
Size: 350 56 255 38 269 398 2011 2531
Fragments arranged by size:
2531 2011 398 350 269 255 56 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2490 2522 4549 4581 4623
Size: 32 135 32 918 32 2027 32 42
Cuts at: 4623 4655 1373
Size: 32 2626
Fragments arranged by size:
2626 2027 918 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3252 3498 669
Size: 988 281 1314 246 3079
Fragments arranged by size:
3079 1314 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5908
HpaI GTT'AAC
Cuts at: 3498 3498
Size: 5908
KasI G'GCGC_C
Cuts at: 2024 2080 2373 2024
Size: 56 293 5559
Fragments arranged by size:
5559 293 56
KpnI G_GTAC'C
Cuts at: 3233 3233
Size: 5908
MfeI C'AATT_G
Cuts at: 3507 3507
Size: 5908
MluI A'CGCG_T
Cuts at: 5784 5784
Size: 5908
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5022 5206 1305
Size: 3717 184 2007
Fragments arranged by size:
3717 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4086
Fragments arranged by size:
4086 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3866 4225 4384 518
Size: 648 2700 359 159 2042
Fragments arranged by size:
2700 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2077 2277 2307 2635 3324 4001 5149 5394
Size: 471 200 30 328 689 677 1148 245
Cuts at: 5394 1606
Size: 2120
Fragments arranged by size:
2120 1148 689 677 471 328 245 200
30
NaeI GCC'GGC
Cuts at: 2103 2103
Size: 5908
NarI GG'CG_CC
Cuts at: 2025 2081 2374 2025
Size: 56 293 5559
Fragments arranged by size:
5559 293 56
NcoI C'CATG_G
Cuts at: 513 1065 2004 2121 513
Size: 552 939 117 4300
Fragments arranged by size:
4300 939 552 117
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5908
NgoMIV G'CCGG_C
Cuts at: 2101 2101
Size: 5908
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5908
NotI GC'GGCC_GC
Cuts at: 2201 3355 2201
Size: 1154 4754
NspI r_CATG'y
Cuts at: 1132 1229 3201 3266 4811 1132
Size: 97 1972 65 1545 2229
Fragments arranged by size:
2229 1972 1545 97 65
PasI CC'CwG_GG
Cuts at: 1348 2923 1348
Size: 1575 4333
PciI A'CATG_T
Cuts at: 3197 4807 3197
Size: 1610 4298
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5908
PfoI T'CCnGG_A
Cuts at: 2591 2591
Size: 5908
PmeI GTTT'AAAC
Cuts at: 3212 3212
Size: 5908
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3977 4009 5490 5522 3977
Size: 32 1481 32 4363
Fragments arranged by size:
4363 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2683 2997 2683
Size: 314 5594
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5908
PsiI TTA'TAA
Cuts at: 3478 3478
Size: 5908
PspOMI G'GGCC_C
Cuts at: 2280 2352 2280
Size: 72 5836
PstI C_TGCA'G
Cuts at: 830 2415 3260 830
Size: 1585 845 3478
Fragments arranged by size:
3478 1585 845
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5908
PvuII CAG'CTG
Cuts at: 2635 2635
Size: 5908
SacI G_AGCT'C
Cuts at: 2459 2991 2459
Size: 532 5376
SacII CC_GC'GG
Cuts at: 2078 2078
Size: 5908
SalI G'TCGA_C
Cuts at: 1936 3250 1936
Size: 1314 4594
SbfI CC_TGCA'GG
Cuts at: 3260 3260
Size: 5908
ScaI AGT'ACT
Cuts at: 2834 4156 5868 2834
Size: 1322 1712 2874
Fragments arranged by size:
2874 1712 1322
SexAI A'CCwGG_T
Cuts at: 3189 3189
Size: 5908
SfcI C'TryA_G
Cuts at: 826 1045 2411 2630 3256 4391 5072 5263 826
Size: 219 1366 219 626 1135 681 191 1471
Fragments arranged by size:
1471 1366 1135 681 626 219 219 191
SfoI GGC'GCC
Cuts at: 2026 2082 2375 2026
Size: 56 293 5559
Fragments arranged by size:
5559 293 56
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5908
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5908
SmaI CCC'GGG
Cuts at: 1872 2117 2148 2285 3235 1872
Size: 245 31 137 950 4545
Fragments arranged by size:
4545 950 245 137 31
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2366 4012 4913 5175 820
Size: 197 926 423 1646 901 262 1553
Fragments arranged by size:
1646 1553 926 901 423 262 197
SnaBI TAC'GTA
Cuts at: 493 3699 493
Size: 3206 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5908
SphI G_CATG'C
Cuts at: 1132 3266 1132
Size: 2134 3774
SrfI GCCC'GGGC
Cuts at: 2117 2117
Size: 5908
SspI AAT'ATT
Cuts at: 5 52 3832 5
Size: 47 3780 2081
Fragments arranged by size:
3780 2081 47
StuI AGG'CCT
Cuts at: 1517 2156 1517
Size: 639 5269
StyI C'CwwG_G
Cuts at: 513 1065 2004 2121 2904 3347 513
Size: 552 939 117 783 443 3074
Fragments arranged by size:
3074 939 783 552 443 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3935 3952 4105 4290 4735 325
Size: 1255 2355 17 153 185 445 1498
Fragments arranged by size:
2355 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2832 4154 5866
Size: 274 80 33 51 1443 855 1322 1712
Cuts at: 5866 96
Size: 138
Fragments arranged by size:
1712 1443 1322 855 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5868 5900 5868
Size: 32 5876
Tth111I GACn'n_nGTC
Cuts at: 1316 3191 1316
Size: 1875 4033
XbaI T'CTAG_A
Cuts at: 3244 3244
Size: 5908
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2128 2737 2128
Size: 609 5299
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5908
XmaI C'CCGG_G
Cuts at: 1870 2115 2146 2283 3233 1870
Size: 245 31 137 950 4545
Fragments arranged by size:
4545 950 245 137 31
XmnI GAAnn'nnTTC
Cuts at: 4037 4037
Size: 5908
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3716 276
Size: 53 83 186 936 2182 2468
Fragments arranged by size:
2468 2182 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FseI
FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII
SanDI SapI SfiI SwaI