Restriction map for
pFN21AE0687
(Circular) (Six-base) MAPSORT of: pFN21AE0687.seq Check: 9223 from: 1 to: 5989
pFN21AE0687 5989 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3799 278
Size: 53 83 186 936 2263 2468
Fragments arranged by size:
2468 2263 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3332 1937
Size: 1395 4594
Acc65I G'GTAC_C
Cuts at: 3310 3310
Size: 5989
AclI AA'CG_TT
Cuts at: 1563 4116 4489 1563
Size: 2553 373 3063
Fragments arranged by size:
3063 2553 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2977 4052 5436 5704 1632
Size: 1345 1075 1384 268 1917
Fragments arranged by size:
1917 1384 1345 1075 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5792
AflIII A'CryG_T
Cuts at: 1203 4888 5865 1203
Size: 3685 977 1327
Fragments arranged by size:
3685 1327 977
AgeI A'CCGG_T
Cuts at: 4820 4820
Size: 5989
AhdI GACnn_n'nnGTC
Cuts at: 4718 4718
Size: 5989
AleI CACnn'nnGTG
Cuts at: 1166 2375 1166
Size: 1209 4780
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2567 1184
Size: 1383 4606
AlwNI CAG_nnn'CTG
Cuts at: 3732 5304 3732
Size: 1572 4417
ApaLI G'TGCA_C
Cuts at: 4046 5202 4046
Size: 1156 4833
ApoI r'AATT_y
Cuts at: 1493 2726 3298 3524 3676 5785 1493
Size: 1233 572 226 152 2109 1697
Fragments arranged by size:
2109 1697 1233 572 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2696 4543 160
Size: 872 1664 1847 1606
Fragments arranged by size:
1847 1664 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5989
AvaI C'yCGr_G
Cuts at: 1870 1943 2063 2451 2833 3314 1870
Size: 73 120 388 382 481 4545
Fragments arranged by size:
4545 481 388 382 120 73
BamHI G'GATC_C
Cuts at: 3319 3686 3319
Size: 367 5622
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2369 2502 2551 3277 3310
Size: 325 217 627 582 133 49 726 33
Cuts at: 3310 4765 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 726 627 582 325 217 133
49 33
BanII G_rGCy'C
Cuts at: 1391 2070 2850 3308 1391
Size: 679 780 458 4072
Fragments arranged by size:
4072 780 679 458
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5018
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2932 2966 4180 4214 1903
Size: 34 995 34 1214 34 3678
Fragments arranged by size:
3678 1214 995 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3881 5091 3881
Size: 1210 4779
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5989
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3011 4600 136
Size: 107 122 71 631 605 1339 1589 1525
Fragments arranged by size:
1589 1525 1339 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5984 5984
Size: 5989
BlpI GC'TnA_GC
Cuts at: 2165 3406 2165
Size: 1241 4748
Bme1580I G_kGCm'C
Cuts at: 1790 2372 4050 5206 1790
Size: 582 1678 1156 2573
Fragments arranged by size:
2573 1678 1156 582
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5989
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3720 4678 442
Size: 416 53 83 399 24 2303 958 1753
Fragments arranged by size:
2303 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2739 1056
Size: 1683 4306
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2864 4649 1239
Size: 1625 1785 2579
Fragments arranged by size:
2579 1785 1625
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2300 4114 4979 5277 2300
Size: 1814 865 298 3012
Fragments arranged by size:
3012 1814 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4652 882
Size: 617 3153 2219
Fragments arranged by size:
3153 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3780 493
Size: 711 2576 2702
Fragments arranged by size:
2702 2576 711
BsaBI GATnn'nnATC
Cuts at: 2987 5983 2987
Size: 2996 2993
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3796 4178 4813 275
Size: 53 83 186 936 2263 382 635 1451
Fragments arranged by size:
2263 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2892 4422 4820 5094 5241 5652 2892
Size: 1530 398 274 147 411 3229
Fragments arranged by size:
3229 1530 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3011 3041 3074 3104 1178
Size: 30 165 30 1608 30 33 30 4063
Fragments arranged by size:
4063 1608 165 33 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2040 2234 3062 3079 3224 3227 1186
Size: 854 194 828 17 145 3 3948
Fragments arranged by size:
3948 854 828 194 145 17 3
BseYI C'CCAG_C
Cuts at: 2173 5192 5688 2173
Size: 3019 496 2474
Fragments arranged by size:
3019 2474 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2153 1163
Size: 573 417 4999
Fragments arranged by size:
4999 573 417
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3439 4200 5228 1540
Size: 275 186 1438 761 1028 2301
Fragments arranged by size:
2301 1438 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2850 4050 4135 5206 2850
Size: 1200 85 1071 3633
Fragments arranged by size:
3633 1200 1071 85
BsmI GAATG_Cn'
Cuts at: 3500 3593 3500
Size: 93 5896
BsmBI CGTCTCn'nnnn_
Cuts at: 2072 2072
Size: 5989
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2070 2372 2850 3308 4050 4135 5206
Size: 399 280 302 478 458 742 85 1071
Cuts at: 5206 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 478 458 399 302 280
85
BspEI T'CCGG_A
Cuts at: 2892 5652 2892
Size: 2760 3229
BspHI T'CATG_A
Cuts at: 2520 3120 3878 5608 2520
Size: 600 758 1730 2901
Fragments arranged by size:
2901 1730 758 600
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2823 3344 844
Size: 1979 521 3489
Fragments arranged by size:
3489 1979 521
BsrBI CCG'CTC
Cuts at: 1394 1823 3876 1394
Size: 429 2053 3507
Fragments arranged by size:
3507 2053 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4484 4658 65
Size: 1183 3236 174 1396
Fragments arranged by size:
3236 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2864 4633 4820 1700
Size: 110 1054 1769 187 2869
Fragments arranged by size:
2869 1769 1054 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5989
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5989
BssSI C'ACGA_G
Cuts at: 4049 5061 4049
Size: 1012 4977
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5989
BstBI TT'CG_AA
Cuts at: 4793 4793
Size: 5989
BstEII G'GTnAC_C
Cuts at: 1184 2227 1184
Size: 1043 4946
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2920 2011
Size: 909 5080
BstYI r'GATC_y
Cuts at: 1972 3319 3686 4071 4088 5540 5626 5638 5984
Size: 1347 367 385 17 1452 86 12 346
Cuts at: 5984 1972
Size: 1977
Fragments arranged by size:
1977 1452 1347 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4498
Fragments arranged by size:
4498 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2817 487
Size: 683 56 1591 3659
Fragments arranged by size:
3659 1591 683 56
BtsI GCAGTG_nn'
Cuts at: 3505 4299 4319 3505
Size: 794 20 5175
Fragments arranged by size:
5175 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5989
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5954
DraI TTT'AAA
Cuts at: 3293 3293
Size: 5989
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4996 809
Size: 507 3680 1802
Fragments arranged by size:
3680 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2922 3150 3436
Size: 54 1425 50 275 18 1092 228 286
Cuts at: 3436 4325 5954 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1092 889 286 275 228 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3436 1537
Size: 275 1624 4090
Fragments arranged by size:
4090 1624 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2285 2972 3919 1353
Size: 932 687 947 3423
Fragments arranged by size:
3423 947 932 687
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3125 4576 4950 5096 387
Size: 1064 138 1536 1451 374 146 1280
Fragments arranged by size:
1536 1451 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2848 2848
Size: 5989
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2864 2977 4052 4649 5436 5704 1239
Size: 393 1232 113 1075 597 787 268 1524
Fragments arranged by size:
1524 1232 1075 787 597 393 268 113
EcoO109I rG'GnC_Cy
Cuts at: 2829 2829
Size: 5989
EcoRI G'AATT_C
Cuts at: 3298 3676 3298
Size: 378 5611
FspI TGC'GCA
Cuts at: 4495 4495
Size: 5989
HaeII r_GCGC'y
Cuts at: 1678 2340 3000 3040 5136 1678
Size: 662 660 40 2096 2531
Fragments arranged by size:
2531 2096 662 660 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3011 3043 4630 4662 4704
Size: 32 135 32 1439 32 1587 32 42
Cuts at: 4704 4736 1373
Size: 32 2626
Fragments arranged by size:
2626 1587 1439 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2017 3100 3333 3579 669
Size: 988 281 79 1083 233 246 3079
Fragments arranged by size:
3079 1083 988 281 246 233 79
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5989
HpaI GTT'AAC
Cuts at: 3579 3579
Size: 5989
KpnI G_GTAC'C
Cuts at: 3314 3314
Size: 5989
MfeI C'AATT_G
Cuts at: 3588 3588
Size: 5989
MluI A'CGCG_T
Cuts at: 5865 5865
Size: 5989
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5103 5287 1305
Size: 3798 184 2007
Fragments arranged by size:
3798 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2924 3152 10
Size: 54 1425 343 1092 228 2847
Fragments arranged by size:
2847 1425 1092 343 228 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2195 2375 3267 3947 4306 4465
Size: 648 843 186 180 892 680 359 159
Cuts at: 4465 518
Size: 2042
Fragments arranged by size:
2042 892 843 680 648 359 186 180
159
MspA1I CmG'CkG
Cuts at: 1606 2129 2784 2857 3405 4082 5230 5475 1606
Size: 523 655 73 548 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 655 548 523 245 73
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4498
Fragments arranged by size:
4498 939 552
NdeI CA'TA_TG
Cuts at: 387 2689 3264 387
Size: 2302 575 3112
Fragments arranged by size:
3112 2302 575
NheI G'CTAG_C
Cuts at: 1052 2735 1052
Size: 1683 4306
NotI GC'GGCC_GC
Cuts at: 3436 3436
Size: 5989
NspI r_CATG'y
Cuts at: 1132 1229 2290 2947 3347 4892 1132
Size: 97 1061 657 400 1545 2229
Fragments arranged by size:
2229 1545 1061 657 400 97
PasI CC'CwG_GG
Cuts at: 1348 3185 1348
Size: 1837 4152
PciI A'CATG_T
Cuts at: 4888 4888
Size: 5989
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2118 3006 1108
Size: 1010 888 4091
Fragments arranged by size:
4091 1010 888
PfoI T'CCnGG_A
Cuts at: 2665 2665
Size: 5989
PmeI GTTT'AAAC
Cuts at: 3293 3293
Size: 5989
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2052 2084 4058 4090 5571 5603 2052
Size: 32 1974 32 1481 32 2438
Fragments arranged by size:
2438 1974 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5989
PsiI TTA'TAA
Cuts at: 3559 3559
Size: 5989
PstI C_TGCA'G
Cuts at: 830 3341 830
Size: 2511 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5989
PvuII CAG'CTG
Cuts at: 2857 2857
Size: 5989
SacI G_AGCT'C
Cuts at: 2850 2850
Size: 5989
SalI G'TCGA_C
Cuts at: 1936 3331 1936
Size: 1395 4594
SbfI CC_TGCA'GG
Cuts at: 3341 3341
Size: 5989
ScaI AGT'ACT
Cuts at: 4237 5949 4237
Size: 1712 4277
SexAI A'CCwGG_T
Cuts at: 2498 2498
Size: 5989
SfcI C'TryA_G
Cuts at: 826 1045 3337 4472 5153 5344 826
Size: 219 2292 1135 681 191 1471
Fragments arranged by size:
2292 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5989
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5989
SmaI CCC'GGG
Cuts at: 1872 2453 3316 1872
Size: 581 863 4545
Fragments arranged by size:
4545 863 581
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2315 4093 4994 5256 820
Size: 197 926 372 1778 901 262 1553
Fragments arranged by size:
1778 1553 926 901 372 262 197
SnaBI TAC'GTA
Cuts at: 493 3780 493
Size: 3287 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5989
SphI G_CATG'C
Cuts at: 1132 2290 2947 3347 1132
Size: 1158 657 400 3774
Fragments arranged by size:
3774 1158 657 400
SspI AAT'ATT
Cuts at: 5 52 3913 5
Size: 47 3861 2081
Fragments arranged by size:
3861 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5989
StyI C'CwwG_G
Cuts at: 513 1065 2004 3428 513
Size: 552 939 1424 3074
Fragments arranged by size:
3074 1424 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2796 4016 4033 4186 4371 4816 325
Size: 1255 1216 1220 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1220 1216 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2145 4235 5947
Size: 274 80 33 51 1443 168 2090 1712
Cuts at: 5947 96
Size: 138
Fragments arranged by size:
2090 1712 1443 274 168 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5949 5981 5949
Size: 32 5957
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5989
XbaI T'CTAG_A
Cuts at: 2413 3325 2413
Size: 912 5077
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2362 2362
Size: 5989
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5989
XmaI C'CCGG_G
Cuts at: 1870 2451 3314 1870
Size: 581 863 4545
Fragments arranged by size:
4545 863 581
XmnI GAAnn'nnTTC
Cuts at: 4118 4118
Size: 5989
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3797 276
Size: 53 83 186 936 2263 2468
Fragments arranged by size:
2468 2263 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI
SwaI