Restriction map for
pFN21AE0683
(Circular) (Six-base) MAPSORT of: pFN21AE0683.seq Check: 4516 from: 1 to: 5527
pFN21AE0683 5527 bp
With 182 enzymes: SgfI *
October 20, 2009 10:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3337 278
Size: 53 83 186 936 1801 2468
Fragments arranged by size:
2468 1801 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2870 1937
Size: 933 4594
Acc65I G'GTAC_C
Cuts at: 2848 2848
Size: 5527
AclI AA'CG_TT
Cuts at: 1563 3654 4027 1563
Size: 2091 373 3063
Fragments arranged by size:
3063 2091 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2345 2789 3590 4974 5242 1632
Size: 713 444 801 1384 268 1917
Fragments arranged by size:
1917 1384 801 713 444 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5330
AflIII A'CryG_T
Cuts at: 1203 4426 5403 1203
Size: 3223 977 1327
Fragments arranged by size:
3223 1327 977
AgeI A'CCGG_T
Cuts at: 4358 4358
Size: 5527
AhdI GACnn_n'nnGTC
Cuts at: 4256 4256
Size: 5527
AleI CACnn'nnGTG
Cuts at: 1166 2060 1166
Size: 894 4633
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5527
AlwNI CAG_nnn'CTG
Cuts at: 2323 3270 4842 2323
Size: 947 1572 3008
Fragments arranged by size:
3008 1572 947
ApaI G_GGCC'C
Cuts at: 2110 2110
Size: 5527
ApaLI G'TGCA_C
Cuts at: 3584 4740 3584
Size: 1156 4371
ApoI r'AATT_y
Cuts at: 1493 2836 3062 3214 5323 1493
Size: 1343 226 152 2109 1697
Fragments arranged by size:
2109 1697 1343 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4081 160
Size: 872 3049 1606
Fragments arranged by size:
3049 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5527
AvaI C'yCGr_G
Cuts at: 1870 1943 2058 2109 2852 1870
Size: 73 115 51 743 4545
Fragments arranged by size:
4545 743 115 73 51
BamHI G'GATC_C
Cuts at: 2857 3224 2857
Size: 367 5160
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2184 2416 2538 2848 4303
Size: 325 217 627 397 232 122 310 1455
Cuts at: 4303 618
Size: 1842
Fragments arranged by size:
1842 1455 627 397 325 310 232 217
122
BanII G_rGCy'C
Cuts at: 1391 2110 2779 2846 1391
Size: 719 669 67 4072
Fragments arranged by size:
4072 719 669 67
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4556
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3718 3752 1903
Size: 34 1781 34 3678
Fragments arranged by size:
3678 1781 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2437 3419 4629 2437
Size: 982 1210 3335
Fragments arranged by size:
3335 1210 982
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5527
BfrBI ATG'CAT
Cuts at: 2683 2683
Size: 5527
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2251 4138 136
Size: 107 122 71 631 605 579 1887 1525
Fragments arranged by size:
1887 1525 631 605 579 122 107 71
BglII A'GATC_T
Cuts at: 2155 2572 5522 2155
Size: 417 2950 2160
Fragments arranged by size:
2950 2160 417
BlpI GC'TnA_GC
Cuts at: 2944 2944
Size: 5527
Bme1580I G_kGCm'C
Cuts at: 1790 2110 2187 2421 2769 3588 4744 1790
Size: 320 77 234 348 819 1156 2573
Fragments arranged by size:
2573 1156 819 348 320 234 77
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5527
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2216 3258 4216
Size: 416 53 83 399 24 799 1042 958
Cuts at: 4216 442
Size: 1753
Fragments arranged by size:
1753 1042 958 799 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5527
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4187 1239
Size: 2948 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2354 2763 3652 4517 4815 2354
Size: 409 889 865 298 3066
Fragments arranged by size:
3066 889 865 409 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4190 882
Size: 617 2691 2219
Fragments arranged by size:
2691 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2764 3318 493
Size: 711 1560 554 2702
Fragments arranged by size:
2702 1560 711 554
BsaBI GATnn'nnATC
Cuts at: 5521 5521
Size: 5527
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3334 3716 4351 275
Size: 53 83 186 936 1801 382 635 1451
Fragments arranged by size:
1801 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3960 4358 4632 4779 5190 3960
Size: 398 274 147 411 4297
Fragments arranged by size:
4297 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5302
Fragments arranged by size:
5302 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2480 1186
Size: 1294 4233
BseYI C'CCAG_C
Cuts at: 2040 4730 5226 2040
Size: 2690 496 2341
Fragments arranged by size:
2690 2341 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2180 1163
Size: 573 444 4510
Fragments arranged by size:
4510 573 444
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2977 3738 4766 1540
Size: 275 186 976 761 1028 2301
Fragments arranged by size:
2301 1028 976 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2779 3588 3673 4744 2779
Size: 809 85 1071 3562
Fragments arranged by size:
3562 1071 809 85
BsmI GAATG_Cn'
Cuts at: 3038 3131 3038
Size: 93 5434
BsmBI CGTCTCn'nnnn_
Cuts at: 2630 2630
Size: 5527
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2110 2187 2421 2769 2779 2846 3588
Size: 399 320 77 234 348 10 67 742
Cuts at: 3588 3673 4744 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 348 320 234 85
77 67 10
BspEI T'CCGG_A
Cuts at: 5190 5190
Size: 5527
BspHI T'CATG_A
Cuts at: 3416 5146 3416
Size: 1730 3797
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2882 844
Size: 2038 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3414 1394
Size: 429 1591 3507
Fragments arranged by size:
3507 1591 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4022 4196 65
Size: 1183 2774 174 1396
Fragments arranged by size:
2774 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4171 4358 1700
Size: 110 2361 187 2869
Fragments arranged by size:
2869 2361 187 110
BsrGI T'GTAC_A
Cuts at: 96 2503 96
Size: 2407 3120
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5527
BssSI C'ACGA_G
Cuts at: 2339 3587 4599 2339
Size: 1248 1012 3267
Fragments arranged by size:
3267 1248 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5527
BstBI TT'CG_AA
Cuts at: 4331 4331
Size: 5527
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5527
BstYI r'GATC_y
Cuts at: 1972 2155 2572 2857 3224 3609 3626 5078 5164
Size: 183 417 285 367 385 17 1452 86
Cuts at: 5164 5176 5522 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 417 385 367 346 285 183
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2610 2610
Size: 5527
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4036
Fragments arranged by size:
4036 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4788
Fragments arranged by size:
4788 683 56
BtsI GCAGTG_nn'
Cuts at: 2412 2462 3043 3837 3857 2412
Size: 50 581 794 20 4082
Fragments arranged by size:
4082 794 581 50 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5527
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2065 2100 529
Size: 35 1501 35 3956
Fragments arranged by size:
3956 1501 35 35
DraI TTT'AAA
Cuts at: 2831 2831
Size: 5527
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4534 809
Size: 507 3218 1802
Fragments arranged by size:
3218 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2252 2974 3863
Size: 54 1425 50 275 18 422 722 889
Cuts at: 3863 5492 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 722 422 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2974 1537
Size: 275 1162 4090
Fragments arranged by size:
4090 1162 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2114 2808 3457 1353
Size: 761 694 649 3423
Fragments arranged by size:
3423 761 694 649
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4114 4488 4634 387
Size: 1064 138 2525 374 146 1280
Fragments arranged by size:
2525 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2777 2777
Size: 5527
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2345 2789 3590 4187 4974 5242 1239
Size: 393 713 444 801 597 787 268 1524
Fragments arranged by size:
1524 801 787 713 597 444 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2099 2431 2099
Size: 332 5195
EcoRI G'AATT_C
Cuts at: 2836 3214 2836
Size: 378 5149
FspI TGC'GCA
Cuts at: 4033 4033
Size: 5527
HaeII r_GCGC'y
Cuts at: 1678 4674 1678
Size: 2996 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2479 2511 2564 2596 4168
Size: 32 135 32 907 32 53 32 1572
Cuts at: 4168 4200 4242 4274 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1572 907 135 53 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2871 3117 669
Size: 988 281 933 246 3079
Fragments arranged by size:
3079 988 933 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5527
HpaI GTT'AAC
Cuts at: 3117 3117
Size: 5527
KpnI G_GTAC'C
Cuts at: 2852 2852
Size: 5527
MfeI C'AATT_G
Cuts at: 3126 3126
Size: 5527
MluI A'CGCG_T
Cuts at: 5403 5403
Size: 5527
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4641 4825 1305
Size: 3336 184 2007
Fragments arranged by size:
3336 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2254 10
Size: 54 1425 343 422 3283
Fragments arranged by size:
3283 1425 422 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2060 2688 3485 3844 4003 518
Size: 648 894 628 797 359 159 2042
Fragments arranged by size:
2042 894 797 648 628 359 159
MspA1I CmG'CkG
Cuts at: 1606 2323 2523 2943 3620 4768 5013 1606
Size: 717 200 420 677 1148 245 2120
Fragments arranged by size:
2120 1148 717 677 420 245 200
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4036
Fragments arranged by size:
4036 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5527
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5527
NotI GC'GGCC_GC
Cuts at: 2974 2974
Size: 5527
NsiI A_TGCA'T
Cuts at: 2685 2685
Size: 5527
NspI r_CATG'y
Cuts at: 1132 1229 2885 4430 1132
Size: 97 1656 1545 2229
Fragments arranged by size:
2229 1656 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2103 2770 1348
Size: 755 667 4105
Fragments arranged by size:
4105 755 667
PciI A'CATG_T
Cuts at: 4426 4426
Size: 5527
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2671 1108
Size: 1563 3964
PmeI GTTT'AAAC
Cuts at: 2831 2831
Size: 5527
PmlI CAC'GTG
Cuts at: 2764 2764
Size: 5527
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3596 3628 5109 5141 3596
Size: 32 1481 32 3982
Fragments arranged by size:
3982 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2099 2431 2099
Size: 332 5195
PshAI GACnn'nnGTC
Cuts at: 1613 2748 1613
Size: 1135 4392
PsiI TTA'TAA
Cuts at: 3097 3097
Size: 5527
PspOMI G'GGCC_C
Cuts at: 2106 2106
Size: 5527
PstI C_TGCA'G
Cuts at: 830 2567 2879 830
Size: 1737 312 3478
Fragments arranged by size:
3478 1737 312
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5527
PvuII CAG'CTG
Cuts at: 2323 2523 2323
Size: 200 5327
SacI G_AGCT'C
Cuts at: 2779 2779
Size: 5527
SalI G'TCGA_C
Cuts at: 1936 2869 1936
Size: 933 4594
SanDI GG'GwC_CC
Cuts at: 2099 2099
Size: 5527
SapI GCTCTTCn'nnn_
Cuts at: 2114 2114
Size: 5527
SbfI CC_TGCA'GG
Cuts at: 2879 2879
Size: 5527
ScaI AGT'ACT
Cuts at: 2226 3775 5487 2226
Size: 1549 1712 2266
Fragments arranged by size:
2266 1712 1549
SfcI C'TryA_G
Cuts at: 826 1045 2435 2563 2875 4010 4691 4882 826
Size: 219 1390 128 312 1135 681 191 1471
Fragments arranged by size:
1471 1390 1135 681 312 219 191 128
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5527
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5527
SmaI CCC'GGG
Cuts at: 1872 2060 2854 1872
Size: 188 794 4545
Fragments arranged by size:
4545 794 188
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2369 2778 3631 4532 4794 820
Size: 197 926 426 409 853 901 262 1553
Fragments arranged by size:
1553 926 901 853 426 409 262 197
SnaBI TAC'GTA
Cuts at: 493 3318 493
Size: 2825 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5527
SphI G_CATG'C
Cuts at: 1132 2885 1132
Size: 1753 3774
SspI AAT'ATT
Cuts at: 5 52 2707 3451 5
Size: 47 2655 744 2081
Fragments arranged by size:
2655 2081 744 47
StuI AGG'CCT
Cuts at: 1517 2337 1517
Size: 820 4707
StyI C'CwwG_G
Cuts at: 513 1065 2004 2421 2966 513
Size: 552 939 417 545 3074
Fragments arranged by size:
3074 939 552 545 417
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2054 2402 2804 3554 3571 3724 3909
Size: 1255 474 348 402 750 17 153 185
Cuts at: 3909 4354 325
Size: 445 1498
Fragments arranged by size:
1498 1255 750 474 445 402 348 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2224 2503 2577
Size: 274 80 33 51 1443 247 279 74
Cuts at: 2577 2808 3773 5485 96
Size: 231 965 1712 138
Fragments arranged by size:
1712 1443 965 279 274 247 231 138
80 74 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2189 2221 5487 5519 2189
Size: 32 3266 32 2197
Fragments arranged by size:
3266 2197 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5527
XbaI T'CTAG_A
Cuts at: 2713 2863 2713
Size: 150 5377
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5527
XmaI C'CCGG_G
Cuts at: 1870 2058 2852 1870
Size: 188 794 4545
Fragments arranged by size:
4545 794 188
XmnI GAAnn'nnTTC
Cuts at: 3656 3656
Size: 5527
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3335 276
Size: 53 83 186 936 1801 2468
Fragments arranged by size:
2468 1801 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BstXI BstZ17I DraIII EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PspXI PsrI RsrII SacII SexAI SfiI
SfoI SrfI SwaI XcmI