Restriction map for
pFN21AE0678
(Circular) (Six-base) MAPSORT of: pFN21AE0678.seq Check: 8365 from: 1 to: 5893
pFN21AE0678 5893 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2346 2346
Size: 5893
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3703 278
Size: 53 83 186 936 2167 2468
Fragments arranged by size:
2468 2167 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3236 1937
Size: 1299 4594
Acc65I G'GTAC_C
Cuts at: 3214 3214
Size: 5893
AclI AA'CG_TT
Cuts at: 1563 4020 4393 1563
Size: 2457 373 3063
Fragments arranged by size:
3063 2457 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3956 5340 5608 1632
Size: 2324 1384 268 1917
Fragments arranged by size:
2324 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5696
AflIII A'CryG_T
Cuts at: 1203 2289 2712 3038 4792 5769 1203
Size: 1086 423 326 1754 977 1327
Fragments arranged by size:
1754 1327 1086 977 423 326
AgeI A'CCGG_T
Cuts at: 4724 4724
Size: 5893
AhdI GACnn_n'nnGTC
Cuts at: 2623 4622 2623
Size: 1999 3894
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5893
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5893
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2276 2308 2276
Size: 32 5861
AlwNI CAG_nnn'CTG
Cuts at: 2236 3636 5208 2236
Size: 1400 1572 2921
Fragments arranged by size:
2921 1572 1400
ApaLI G'TGCA_C
Cuts at: 2483 3950 5106 2483
Size: 1467 1156 3270
Fragments arranged by size:
3270 1467 1156
ApoI r'AATT_y
Cuts at: 1493 2076 3202 3428 3580 5689 1493
Size: 583 1126 226 152 2109 1697
Fragments arranged by size:
2109 1697 1126 583 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4447 160
Size: 872 3415 1606
Fragments arranged by size:
3415 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5893
AvaI C'yCGr_G
Cuts at: 1870 1943 3218 1870
Size: 73 1275 4545
Fragments arranged by size:
4545 1275 73
BamHI G'GATC_C
Cuts at: 3223 3590 3223
Size: 367 5526
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2549 2566 3214 4669 618
Size: 325 217 627 762 17 648 1455 1842
Fragments arranged by size:
1842 1455 762 648 627 325 217 17
BanII G_rGCy'C
Cuts at: 1391 3212 1391
Size: 1821 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2200 2292 3035 928
Size: 971 301 92 743 3786
Fragments arranged by size:
3786 971 743 301 92
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4084 4118 1903
Size: 34 2147 34 3678
Fragments arranged by size:
3678 2147 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3785 4995 3785
Size: 1210 4683
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5893
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4504 136
Size: 107 122 71 631 605 2832 1525
Fragments arranged by size:
2832 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5888 5888
Size: 5893
BlpI GC'TnA_GC
Cuts at: 3310 3310
Size: 5893
Bme1580I G_kGCm'C
Cuts at: 1790 2481 2487 3954 5110 1790
Size: 691 6 1467 1156 2573
Fragments arranged by size:
2573 1467 1156 691 6
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5893
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2869 3624 4582
Size: 416 53 83 399 24 1452 755 958
Cuts at: 4582 442
Size: 1753
Fragments arranged by size:
1753 1452 958 755 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5893
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2204 2258 2569 2668 4553 1239
Size: 965 54 311 99 1885 2579
Fragments arranged by size:
2579 1885 965 311 99 54
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2963 4018 4883 5181 2963
Size: 1055 865 298 3675
Fragments arranged by size:
3675 1055 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2316 2659 4556 882
Size: 617 817 343 1897 2219
Fragments arranged by size:
2219 1897 817 617 343
BsaAI yAC'GTr
Cuts at: 493 1204 2482 3684 493
Size: 711 1278 1202 2702
Fragments arranged by size:
2702 1278 1202 711
BsaBI GATnn'nnATC
Cuts at: 5887 5887
Size: 5893
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3700 4082 4717 275
Size: 53 83 186 936 2167 382 635 1451
Fragments arranged by size:
2167 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4326 4724 4998 5145 5556 4326
Size: 398 274 147 411 4663
Fragments arranged by size:
4663 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5668
Fragments arranged by size:
5668 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5893
BseYI C'CCAG_C
Cuts at: 2314 5096 5592 2314
Size: 2782 496 2615
Fragments arranged by size:
2782 2615 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5320
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3343 4104 5132 1540
Size: 275 186 1342 761 1028 2301
Fragments arranged by size:
2301 1342 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2244 2487 3057 3954 4039 5110 2244
Size: 243 570 897 85 1071 3027
Fragments arranged by size:
3027 1071 897 570 243 85
BsmI GAATG_Cn'
Cuts at: 3404 3497 3404
Size: 93 5800
BsmBI CGTCTCn'nnnn_
Cuts at: 2714 2714
Size: 5893
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2244 2481 2487 3057 3212 3954 4039
Size: 399 454 237 6 570 155 742 85
Cuts at: 4039 5110 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 570 454 399 237 155
85 6
BspEI T'CCGG_A
Cuts at: 5556 5556
Size: 5893
BspHI T'CATG_A
Cuts at: 3782 5512 3782
Size: 1730 4163
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2334 2346 2421 3248 844
Size: 1490 12 75 827 3489
Fragments arranged by size:
3489 1490 827 75 12
BsrBI CCG'CTC
Cuts at: 1394 1823 3780 1394
Size: 429 1957 3507
Fragments arranged by size:
3507 1957 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2160 2197 2502 4388 4562 65
Size: 1183 912 37 305 1886 174 1396
Fragments arranged by size:
1886 1396 1183 912 305 174 37
BsrFI r'CCGG_y
Cuts at: 1700 1810 4537 4724 1700
Size: 110 2727 187 2869
Fragments arranged by size:
2869 2727 187 110
BsrGI T'GTAC_A
Cuts at: 96 2122 2707 96
Size: 2026 585 3282
Fragments arranged by size:
3282 2026 585
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5893
BssSI C'ACGA_G
Cuts at: 3953 4965 3953
Size: 1012 4881
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5893
BstBI TT'CG_AA
Cuts at: 4697 4697
Size: 5893
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5893
BstYI r'GATC_y
Cuts at: 1972 3223 3590 3975 3992 5444 5530 5542 5888
Size: 1251 367 385 17 1452 86 12 346
Cuts at: 5888 1972
Size: 1977
Fragments arranged by size:
1977 1452 1251 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2541 513
Size: 552 939 537 3865
Fragments arranged by size:
3865 939 552 537
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2525 487
Size: 683 56 1299 3855
Fragments arranged by size:
3855 1299 683 56
BtsI GCAGTG_nn'
Cuts at: 3409 4203 4223 3409
Size: 794 20 5079
Fragments arranged by size:
5079 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5893
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5858
DraI TTT'AAA
Cuts at: 3197 3197
Size: 5893
DraIII CAC_nnn'GTG
Cuts at: 3167 3167
Size: 5893
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4900 809
Size: 507 3584 1802
Fragments arranged by size:
3584 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3340 4229 5858
Size: 54 1425 50 275 18 1510 889 1629
Cuts at: 5858 8
Size: 43
Fragments arranged by size:
1629 1510 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3340 1537
Size: 275 1528 4090
Fragments arranged by size:
4090 1528 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2384 2873 3823 1353
Size: 1031 489 950 3423
Fragments arranged by size:
3423 1031 950 489
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4480 4854 5000 387
Size: 1064 138 2891 374 146 1280
Fragments arranged by size:
2891 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2204 2258 2569 2668 3956 4553 5340
Size: 393 572 54 311 99 1288 597 787
Cuts at: 5340 5608 1239
Size: 268 1524
Fragments arranged by size:
1524 1288 787 597 572 393 311 268
99 54
EcoNI CCTnn'n_nnAGG
Cuts at: 2907 2907
Size: 5893
EcoRI G'AATT_C
Cuts at: 3202 3580 3202
Size: 378 5515
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3074 3074
Size: 5893
FspI TGC'GCA
Cuts at: 4399 4399
Size: 5893
HaeII r_GCGC'y
Cuts at: 1678 5040 1678
Size: 3362 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2609 2641 2656 2688 4534
Size: 32 135 32 1037 32 15 32 1846
Cuts at: 4534 4566 4608 4640 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1846 1037 135 42 32 32 32
32 32 32 15
HincII GTy'rAC
Cuts at: 669 1657 1938 2059 2617 3237 3483 669
Size: 988 281 121 558 620 246 3079
Fragments arranged by size:
3079 988 620 558 281 246 121
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5893
HpaI GTT'AAC
Cuts at: 3483 3483
Size: 5893
KpnI G_GTAC'C
Cuts at: 3218 3218
Size: 5893
MfeI C'AATT_G
Cuts at: 3492 3492
Size: 5893
MluI A'CGCG_T
Cuts at: 5769 5769
Size: 5893
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5007 5191 1305
Size: 3702 184 2007
Fragments arranged by size:
3702 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4071
Fragments arranged by size:
4071 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2294 2300 3851 4210 4369 518
Size: 648 1128 6 1551 359 159 2042
Fragments arranged by size:
2042 1551 1128 648 359 159 6
MspA1I CmG'CkG
Cuts at: 1606 3309 3986 5134 5379 1606
Size: 1703 677 1148 245 2120
Fragments arranged by size:
2120 1703 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2541 513
Size: 552 939 537 3865
Fragments arranged by size:
3865 939 552 537
NdeI CA'TA_TG
Cuts at: 387 2644 387
Size: 2257 3636
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5893
NotI GC'GGCC_GC
Cuts at: 3340 3340
Size: 5893
NspI r_CATG'y
Cuts at: 1132 1229 2293 3042 3251 4796 1132
Size: 97 1064 749 209 1545 2229
Fragments arranged by size:
2229 1545 1064 749 209 97
PasI CC'CwG_GG
Cuts at: 1348 2520 2629 1348
Size: 1172 109 4612
Fragments arranged by size:
4612 1172 109
PciI A'CATG_T
Cuts at: 2289 3038 4792 2289
Size: 749 1754 3390
Fragments arranged by size:
3390 1754 749
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5893
PmeI GTTT'AAAC
Cuts at: 3197 3197
Size: 5893
PmlI CAC'GTG
Cuts at: 2482 2482
Size: 5893
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3962 3994 5475 5507 3962
Size: 32 1481 32 4348
Fragments arranged by size:
4348 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5893
PsiI TTA'TAA
Cuts at: 3463 3463
Size: 5893
PstI C_TGCA'G
Cuts at: 830 2418 3245 830
Size: 1588 827 3478
Fragments arranged by size:
3478 1588 827
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5893
SalI G'TCGA_C
Cuts at: 1936 3235 1936
Size: 1299 4594
SapI GCTCTTCn'nnn_
Cuts at: 2384 2384
Size: 5893
SbfI CC_TGCA'GG
Cuts at: 3245 3245
Size: 5893
ScaI AGT'ACT
Cuts at: 2689 4141 5853 2689
Size: 1452 1712 2729
Fragments arranged by size:
2729 1712 1452
SfcI C'TryA_G
Cuts at: 826 1045 2414 3241 4376 5057 5248 826
Size: 219 1369 827 1135 681 191 1471
Fragments arranged by size:
1471 1369 1135 827 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5893
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5893
SmaI CCC'GGG
Cuts at: 1872 3220 1872
Size: 1348 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2942 3997 4898 5160 820
Size: 197 926 999 1055 901 262 1553
Fragments arranged by size:
1553 1055 999 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3684 493
Size: 3191 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5893
SphI G_CATG'C
Cuts at: 1132 3251 1132
Size: 2119 3774
SspI AAT'ATT
Cuts at: 5 52 2458 3817 5
Size: 47 2406 1359 2081
Fragments arranged by size:
2406 2081 1359 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5893
StyI C'CwwG_G
Cuts at: 513 1065 2004 2541 3141 3332 513
Size: 552 939 537 600 191 3074
Fragments arranged by size:
3074 939 600 552 537 191
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2306 3920 3937 4090 4275 4720 325
Size: 1255 726 1614 17 153 185 445 1498
Fragments arranged by size:
1614 1498 1255 726 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2122 2160 2687
Size: 274 80 33 51 1443 145 38 527
Cuts at: 2687 2707 4139 5851 96
Size: 20 1432 1712 138
Fragments arranged by size:
1712 1443 1432 527 274 145 138 80
51 38 33 20
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5853 5885 5853
Size: 32 5861
Tth111I GACn'n_nGTC
Cuts at: 1316 3065 1316
Size: 1749 4144
XbaI T'CTAG_A
Cuts at: 3229 3229
Size: 5893
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2560 2560
Size: 5893
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5893
XmaI C'CCGG_G
Cuts at: 1870 3218 1870
Size: 1348 4545
XmnI GAAnn'nnTTC
Cuts at: 2378 3077 4022 2378
Size: 699 945 4249
Fragments arranged by size:
4249 945 699
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3701 276
Size: 53 83 186 936 2167 2468
Fragments arranged by size:
2468 2167 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PstI PvuI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI