Restriction map for
pFN21AE0676
(Circular) (Six-base) MAPSORT of: pFN21AE0676.seq Check: 7603 from: 1 to: 5893
pFN21AE0676 5893 bp
With 182 enzymes: SgfI *
October 20, 2009 10:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3703 278
Size: 53 83 186 936 2167 2468
Fragments arranged by size:
2468 2167 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2356 3236 1937
Size: 419 880 4594
Fragments arranged by size:
4594 880 419
Acc65I G'GTAC_C
Cuts at: 3214 3214
Size: 5893
AclI AA'CG_TT
Cuts at: 1563 4020 4393 1563
Size: 2457 373 3063
Fragments arranged by size:
3063 2457 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2163 2339 2402 3956 5340 5608 1632
Size: 531 176 63 1554 1384 268 1917
Fragments arranged by size:
1917 1554 1384 531 268 176 63
AflII C'TTAA_G
Cuts at: 820 1017 2479 820
Size: 197 1462 4234
Fragments arranged by size:
4234 1462 197
AflIII A'CryG_T
Cuts at: 1203 4792 5769 1203
Size: 3589 977 1327
Fragments arranged by size:
3589 1327 977
AgeI A'CCGG_T
Cuts at: 4724 4724
Size: 5893
AhdI GACnn_n'nnGTC
Cuts at: 4622 4622
Size: 5893
AleI CACnn'nnGTG
Cuts at: 1166 2706 1166
Size: 1540 4353
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5893
AlwNI CAG_nnn'CTG
Cuts at: 3636 5208 3636
Size: 1572 4321
ApaLI G'TGCA_C
Cuts at: 3950 5106 3950
Size: 1156 4737
ApoI r'AATT_y
Cuts at: 1493 2173 2289 2484 2681 2959 3202 3428 3580
Size: 680 116 195 197 278 243 226 152
Cuts at: 3580 5689 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 680 278 243 226 197 195
152 116
AseI AT'TA_AT
Cuts at: 160 1032 4447 160
Size: 872 3415 1606
Fragments arranged by size:
3415 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5893
AvaI C'yCGr_G
Cuts at: 1870 1943 3218 1870
Size: 73 1275 4545
Fragments arranged by size:
4545 1275 73
BamHI G'GATC_C
Cuts at: 3223 3590 3223
Size: 367 5526
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3214 4669 618
Size: 325 217 627 1427 1455 1842
Fragments arranged by size:
1842 1455 1427 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2045 3212 1391
Size: 654 1167 4072
Fragments arranged by size:
4072 1167 654
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2041 2655 928
Size: 971 142 614 4166
Fragments arranged by size:
4166 971 614 142
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4084 4118 1903
Size: 34 50 34 2063 34 3678
Fragments arranged by size:
3678 2063 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3785 4995 3785
Size: 1210 4683
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5893
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2142 4504 136
Size: 107 122 71 631 605 470 2362 1525
Fragments arranged by size:
2362 1525 631 605 470 122 107 71
BglII A'GATC_T
Cuts at: 5888 5888
Size: 5893
BlpI GC'TnA_GC
Cuts at: 2474 3310 2474
Size: 836 5057
Bme1580I G_kGCm'C
Cuts at: 1790 3954 5110 1790
Size: 2164 1156 2573
Fragments arranged by size:
2573 2164 1156
BmgBI CAC'GTC
Cuts at: 1318 2726 1318
Size: 1408 4485
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2220 3624 4582
Size: 416 53 83 399 24 803 1404 958
Cuts at: 4582 442
Size: 1753
Fragments arranged by size:
1753 1404 958 803 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5893
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4553 1239
Size: 3314 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2010 4018 4883 5181 2010
Size: 2008 865 298 2722
Fragments arranged by size:
2722 2008 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4556 882
Size: 617 3057 2219
Fragments arranged by size:
3057 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2966 3684 493
Size: 711 1762 718 2702
Fragments arranged by size:
2702 1762 718 711
BsaBI GATnn'nnATC
Cuts at: 5887 5887
Size: 5893
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3700 4082 4717 275
Size: 53 83 186 936 2167 382 635 1451
Fragments arranged by size:
2167 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4326 4724 4998 5145 5556 4326
Size: 398 274 147 411 4663
Fragments arranged by size:
4663 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5668
Fragments arranged by size:
5668 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3161 1186
Size: 1975 3918
BseYI C'CCAG_C
Cuts at: 5096 5592 5096
Size: 496 5397
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5320
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3343 4104 5132 1540
Size: 275 186 1342 761 1028 2301
Fragments arranged by size:
2301 1342 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2045 3954 4039 5110 2045
Size: 1909 85 1071 2828
Fragments arranged by size:
2828 1909 1071 85
BsmI GAATG_Cn'
Cuts at: 3404 3497 3404
Size: 93 5800
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2045 3212 3954 4039 5110 1391
Size: 399 255 1167 742 85 1071 2174
Fragments arranged by size:
2174 1167 1071 742 399 255 85
BspEI T'CCGG_A
Cuts at: 5556 5556
Size: 5893
BspHI T'CATG_A
Cuts at: 3782 5512 3782
Size: 1730 4163
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3248 844
Size: 2404 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3780 1394
Size: 429 1957 3507
Fragments arranged by size:
3507 1957 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4388 4562 65
Size: 1183 3140 174 1396
Fragments arranged by size:
3140 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4537 4724 1700
Size: 110 2727 187 2869
Fragments arranged by size:
2869 2727 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5893
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5893
BssSI C'ACGA_G
Cuts at: 2010 2510 2686 3953 4965 2010
Size: 500 176 1267 1012 2938
Fragments arranged by size:
2938 1267 1012 500 176
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5893
BstBI TT'CG_AA
Cuts at: 4697 4697
Size: 5893
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5893
BstXI CCAn_nnnn'nTGG
Cuts at: 2911 2911
Size: 5893
BstYI r'GATC_y
Cuts at: 1972 2803 3223 3590 3975 3992 5444 5530 5542
Size: 831 420 367 385 17 1452 86 12
Cuts at: 5542 5888 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 831 420 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5341
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5154
Fragments arranged by size:
5154 683 56
BtsI GCAGTG_nn'
Cuts at: 2200 3409 4203 4223 2200
Size: 1209 794 20 3870
Fragments arranged by size:
3870 1209 794 20
ClaI AT'CG_AT
Cuts at: 1554 2694 1554
Size: 1140 4753
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5858
DraI TTT'AAA
Cuts at: 3197 3197
Size: 5893
DraIII CAC_nnn'GTG
Cuts at: 2207 2207
Size: 5893
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4900 809
Size: 507 3584 1802
Fragments arranged by size:
3584 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2134 2773 3340
Size: 54 1425 50 275 18 304 639 567
Cuts at: 3340 4229 5858 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 639 567 304 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3340 1537
Size: 275 1528 4090
Fragments arranged by size:
4090 1528 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3823 1353
Size: 2470 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4480 4854 5000 387
Size: 1064 138 2891 374 146 1280
Fragments arranged by size:
2891 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2043 2043
Size: 5893
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2163 2339 2402 3956 4553 5340 5608
Size: 393 531 176 63 1554 597 787 268
Cuts at: 5608 1239
Size: 1524
Fragments arranged by size:
1554 1524 787 597 531 393 268 176
63
EcoRI G'AATT_C
Cuts at: 2173 2484 2681 3202 3580 2173
Size: 311 197 521 378 4486
Fragments arranged by size:
4486 521 378 311 197
EcoRV GAT'ATC
Cuts at: 2536 2536
Size: 5893
FspI TGC'GCA
Cuts at: 4399 4399
Size: 5893
HaeII r_GCGC'y
Cuts at: 1678 5040 1678
Size: 3362 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2383 2415 4534 4566 4608
Size: 32 135 32 811 32 2119 32 42
Cuts at: 4608 4640 1373
Size: 32 2626
Fragments arranged by size:
2626 2119 811 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3237 3483 669
Size: 988 281 1299 246 3079
Fragments arranged by size:
3079 1299 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2476 748
Size: 1728 4165
HpaI GTT'AAC
Cuts at: 3483 3483
Size: 5893
KpnI G_GTAC'C
Cuts at: 3218 3218
Size: 5893
MfeI C'AATT_G
Cuts at: 3492 3492
Size: 5893
MluI A'CGCG_T
Cuts at: 5769 5769
Size: 5893
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2059 5007 5191 1305
Size: 754 2948 184 2007
Fragments arranged by size:
2948 2007 754 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2136 2775 10
Size: 54 1425 343 304 639 3128
Fragments arranged by size:
3128 1425 639 343 304 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2514 2520 2706 3851 4210 4369 518
Size: 648 1348 6 186 1145 359 159 2042
Fragments arranged by size:
2042 1348 1145 648 359 186 159 6
MspA1I CmG'CkG
Cuts at: 1606 3309 3986 5134 5379 1606
Size: 1703 677 1148 245 2120
Fragments arranged by size:
2120 1703 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5341
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5893
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5893
NotI GC'GGCC_GC
Cuts at: 3340 3340
Size: 5893
NspI r_CATG'y
Cuts at: 1132 1229 3251 4796 1132
Size: 97 2022 1545 2229
Fragments arranged by size:
2229 2022 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5893
PciI A'CATG_T
Cuts at: 4792 4792
Size: 5893
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5893
PmeI GTTT'AAAC
Cuts at: 3197 3197
Size: 5893
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3962 3994 5475 5507 3962
Size: 32 1481 32 4348
Fragments arranged by size:
4348 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5893
PsiI TTA'TAA
Cuts at: 3463 3463
Size: 5893
PstI C_TGCA'G
Cuts at: 830 3245 830
Size: 2415 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5893
SacI G_AGCT'C
Cuts at: 2045 2045
Size: 5893
SalI G'TCGA_C
Cuts at: 1936 3235 1936
Size: 1299 4594
SbfI CC_TGCA'GG
Cuts at: 3245 3245
Size: 5893
ScaI AGT'ACT
Cuts at: 4141 5853 4141
Size: 1712 4181
SexAI A'CCwGG_T
Cuts at: 2577 2890 3106 2577
Size: 313 216 5364
Fragments arranged by size:
5364 313 216
SfcI C'TryA_G
Cuts at: 826 1045 2994 3241 4376 5057 5248 826
Size: 219 1949 247 1135 681 191 1471
Fragments arranged by size:
1949 1471 1135 681 247 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5893
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5893
SmaI CCC'GGG
Cuts at: 1872 3220 1872
Size: 1348 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2025 2479 3997 4898 5160 820
Size: 197 926 82 454 1518 901 262 1553
Fragments arranged by size:
1553 1518 926 901 454 262 197 82
SnaBI TAC'GTA
Cuts at: 493 3684 493
Size: 3191 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5893
SphI G_CATG'C
Cuts at: 1132 3251 1132
Size: 2119 3774
SspI AAT'ATT
Cuts at: 5 52 2848 3817 5
Size: 47 2796 969 2081
Fragments arranged by size:
2796 2081 969 47
StuI AGG'CCT
Cuts at: 1517 2543 1517
Size: 1026 4867
StyI C'CwwG_G
Cuts at: 513 1065 2137 2783 3332 513
Size: 552 1072 646 549 3074
Fragments arranged by size:
3074 1072 646 552 549
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2318 3920 3937 4090 4275 4720 325
Size: 1255 738 1602 17 153 185 445 1498
Fragments arranged by size:
1602 1498 1255 738 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2583 4139 5851
Size: 274 80 33 51 1443 606 1556 1712
Cuts at: 5851 96
Size: 138
Fragments arranged by size:
1712 1556 1443 606 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5853 5885 5853
Size: 32 5861
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5893
XbaI T'CTAG_A
Cuts at: 3229 3229
Size: 5893
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2144 2768 2144
Size: 624 5269
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5893
XmaI C'CCGG_G
Cuts at: 1870 3218 1870
Size: 1348 4545
XmnI GAAnn'nnTTC
Cuts at: 2505 2628 4022 2505
Size: 123 1394 4376
Fragments arranged by size:
4376 1394 123
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3701 276
Size: 53 83 186 936 2167 2468
Fragments arranged by size:
2468 2167 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacII SanDI SapI SfiI
SfoI SrfI SwaI