Restriction map for
pFN21AE0672
(Circular) (Six-base) MAPSORT of: pFN21AE0672.seq Check: 8619 from: 1 to: 5734
pFN21AE0672 5734 bp
With 182 enzymes: SgfI *
October 20, 2009 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2792 2792
Size: 5734
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3544 278
Size: 53 83 186 936 2008 2468
Fragments arranged by size:
2468 2008 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2046 3077 1937
Size: 109 1031 4594
Fragments arranged by size:
4594 1031 109
Acc65I G'GTAC_C
Cuts at: 2628 3055 2628
Size: 427 5307
AclI AA'CG_TT
Cuts at: 1563 3861 4234 1563
Size: 2298 373 3063
Fragments arranged by size:
3063 2298 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2036 2650 3797 5181 5449 1632
Size: 404 614 1147 1384 268 1917
Fragments arranged by size:
1917 1384 1147 614 404 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5537
AflIII A'CryG_T
Cuts at: 1203 4633 5610 1203
Size: 3430 977 1327
Fragments arranged by size:
3430 1327 977
AgeI A'CCGG_T
Cuts at: 4565 4565
Size: 5734
AhdI GACnn_n'nnGTC
Cuts at: 4463 4463
Size: 5734
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5734
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5734
AlwNI CAG_nnn'CTG
Cuts at: 2677 3477 5049 2677
Size: 800 1572 3362
Fragments arranged by size:
3362 1572 800
ApaI G_GGCC'C
Cuts at: 2596 2596
Size: 5734
ApaLI G'TGCA_C
Cuts at: 2402 3791 4947 2402
Size: 1389 1156 3189
Fragments arranged by size:
3189 1389 1156
ApoI r'AATT_y
Cuts at: 1493 3043 3269 3421 5530 1493
Size: 1550 226 152 2109 1697
Fragments arranged by size:
2109 1697 1550 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4288 160
Size: 872 3256 1606
Fragments arranged by size:
3256 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5734
AvaI C'yCGr_G
Cuts at: 1870 1943 2071 3059 1870
Size: 73 128 988 4545
Fragments arranged by size:
4545 988 128 73
BamHI G'GATC_C
Cuts at: 3064 3431 3064
Size: 367 5367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2075 2369 2513 2628 2748
Size: 325 217 627 288 294 144 115 120
Cuts at: 2748 2802 3055 4510 618
Size: 54 253 1455 1842
Fragments arranged by size:
1842 1455 627 325 294 288 253 217
144 120 115 54
BanII G_rGCy'C
Cuts at: 1391 2114 2252 2553 2596 2664 3053 1391
Size: 723 138 301 43 68 389 4072
Fragments arranged by size:
4072 723 389 301 138 68 43
BbeI G_GCGC'C
Cuts at: 2373 2373
Size: 5734
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4763
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3925 3959 1903
Size: 34 50 34 1904 34 3678
Fragments arranged by size:
3678 1904 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3626 4836 3626
Size: 1210 4524
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5734
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3003 4345 136
Size: 107 122 71 631 605 1331 1342 1525
Fragments arranged by size:
1525 1342 1331 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5729 5729
Size: 5734
BlpI GC'TnA_GC
Cuts at: 3151 3151
Size: 5734
Bme1580I G_kGCm'C
Cuts at: 1790 2406 2596 3795 4951 1790
Size: 616 190 1199 1156 2573
Fragments arranged by size:
2573 1199 1156 616 190
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5734
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3465 4423 442
Size: 416 53 83 399 24 2048 958 1753
Fragments arranged by size:
2048 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5734
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2128 2338 2716 2986 2128
Size: 210 378 270 4876
Fragments arranged by size:
4876 378 270 210
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2252 2777 4394 1239
Size: 1013 525 1617 2579
Fragments arranged by size:
2579 1617 1013 525
Bpu10I CC'TnA_GC
Cuts at: 2658 2658
Size: 5734
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2117 2399 3859 4724 5022 2117
Size: 282 1460 865 298 2829
Fragments arranged by size:
2829 1460 865 298 282
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2473 2757 4397 882
Size: 617 974 284 1640 2219
Fragments arranged by size:
2219 1640 974 617 284
BsaAI yAC'GTr
Cuts at: 493 1204 3525 493
Size: 711 2321 2702
Fragments arranged by size:
2702 2321 711
BsaBI GATnn'nnATC
Cuts at: 5728 5728
Size: 5734
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2370 2601 3541 3923
Size: 53 83 186 936 837 231 940 382
Cuts at: 3923 4558 275
Size: 635 1451
Fragments arranged by size:
1451 940 936 837 635 382 231 186
83 53
BsaWI w'CCGG_w
Cuts at: 2868 2934 4167 4565 4839 4986 5397 2868
Size: 66 1233 398 274 147 411 3205
Fragments arranged by size:
3205 1233 411 398 274 147 66
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5509
Fragments arranged by size:
5509 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5734
BseYI C'CCAG_C
Cuts at: 2498 2941 4937 5433 2498
Size: 443 1996 496 2799
Fragments arranged by size:
2799 1996 496 443
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2369 1163
Size: 573 633 4528
Fragments arranged by size:
4528 633 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3184 3945 4973 1540
Size: 275 186 1183 761 1028 2301
Fragments arranged by size:
2301 1183 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2199 2406 2553 2664 3795 3880 4951 2199
Size: 207 147 111 1131 85 1071 2982
Fragments arranged by size:
2982 1131 1071 207 147 111 85
BsmI GAATG_Cn'
Cuts at: 3245 3338 3245
Size: 93 5641
BsmBI CGTCTCn'nnnn_
Cuts at: 2380 2380
Size: 5734
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2114 2199 2252 2406 2553 2596 2664
Size: 399 324 85 53 154 147 43 68
Cuts at: 2664 3053 3795 3880 4951 1391
Size: 389 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 389 324 154 147
85 85 68 53 43
BspEI T'CCGG_A
Cuts at: 2868 2934 5397 2868
Size: 66 2463 3205
Fragments arranged by size:
3205 2463 66
BspHI T'CATG_A
Cuts at: 3623 5353 3623
Size: 1730 4004
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2391 2792 3089 844
Size: 1547 401 297 3489
Fragments arranged by size:
3489 1547 401 297
BsrBI CCG'CTC
Cuts at: 1394 1823 2107 3621 1394
Size: 429 284 1514 3507
Fragments arranged by size:
3507 1514 429 284
BsrDI GCAATG_nn'
Cuts at: 65 1248 4229 4403 65
Size: 1183 2981 174 1396
Fragments arranged by size:
2981 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4378 4565 1700
Size: 110 2568 187 2869
Fragments arranged by size:
2869 2568 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5734
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5734
BssSI C'ACGA_G
Cuts at: 2123 2243 3794 4806 2123
Size: 120 1551 1012 3051
Fragments arranged by size:
3051 1551 1012 120
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5734
BstBI TT'CG_AA
Cuts at: 4538 4538
Size: 5734
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5734
BstXI CCAn_nnnn'nTGG
Cuts at: 2278 2350 2278
Size: 72 5662
BstYI r'GATC_y
Cuts at: 1972 2052 2673 2811 2865 2949 3064 3431 3816
Size: 80 621 138 54 84 115 367 385
Cuts at: 3816 3833 5285 5371 5383 5729 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 621 385 367 346 138 115
86 84 80 54 17 12
Bsu36I CC'TnA_GG
Cuts at: 2678 2678
Size: 5734
BtgI C'CryG_G
Cuts at: 513 1065 2271 513
Size: 552 1206 3976
Fragments arranged by size:
3976 1206 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4995
Fragments arranged by size:
4995 683 56
BtsI GCAGTG_nn'
Cuts at: 2199 3250 4044 4064 2199
Size: 1051 794 20 3869
Fragments arranged by size:
3869 1051 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5734
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2428 2463 529
Size: 35 1864 35 3800
Fragments arranged by size:
3800 1864 35 35
DraI TTT'AAA
Cuts at: 3038 3038
Size: 5734
DraIII CAC_nnn'GTG
Cuts at: 2125 2125
Size: 5734
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4741 809
Size: 507 3425 1802
Fragments arranged by size:
3425 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2268 3016 3181
Size: 54 1425 50 275 18 438 748 165
Cuts at: 3181 4070 5699 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 748 438 275 165 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3181 1537
Size: 275 1369 4090
Fragments arranged by size:
4090 1369 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2669 2768 3664 1353
Size: 1316 99 896 3423
Fragments arranged by size:
3423 1316 896 99
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4321 4695 4841 387
Size: 1064 138 2732 374 146 1280
Fragments arranged by size:
2732 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2551 2662 2551
Size: 111 5623
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2036 2252 2650 2777 3797 4394 5181
Size: 393 404 216 398 127 1020 597 787
Cuts at: 5181 5449 1239
Size: 268 1524
Fragments arranged by size:
1524 1020 787 597 404 398 393 268
216 127
EcoNI CCTnn'n_nnAGG
Cuts at: 2833 2833
Size: 5734
EcoO109I rG'GnC_Cy
Cuts at: 2152 2170 2362 2410 2526 2593 2967 2152
Size: 18 192 48 116 67 374 4919
Fragments arranged by size:
4919 374 192 116 67 48 18
EcoRI G'AATT_C
Cuts at: 3043 3421 3043
Size: 378 5356
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2920 2920
Size: 5734
FspI TGC'GCA
Cuts at: 4240 4240
Size: 5734
HaeII r_GCGC'y
Cuts at: 1678 2373 4881 1678
Size: 695 2508 2531
Fragments arranged by size:
2531 2508 695
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2705 2737 4375 4407 4449
Size: 32 135 32 1133 32 1638 32 42
Cuts at: 4449 4481 1373
Size: 32 2626
Fragments arranged by size:
2626 1638 1133 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3078 3324 669
Size: 988 281 1140 246 3079
Fragments arranged by size:
3079 1140 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5734
HpaI GTT'AAC
Cuts at: 3324 3324
Size: 5734
KasI G'GCGC_C
Cuts at: 2369 2369
Size: 5734
KpnI G_GTAC'C
Cuts at: 2632 3059 2632
Size: 427 5307
MfeI C'AATT_G
Cuts at: 3333 3333
Size: 5734
MluI A'CGCG_T
Cuts at: 5610 5610
Size: 5734
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4848 5032 1305
Size: 3543 184 2007
Fragments arranged by size:
3543 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2270 3018 10
Size: 54 1425 343 438 748 2726
Fragments arranged by size:
2726 1425 748 438 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3692 4051 4210 518
Size: 648 2526 359 159 2042
Fragments arranged by size:
2526 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3150 3827 4975 5220 1606
Size: 1544 677 1148 245 2120
Fragments arranged by size:
2120 1544 1148 677 245
NarI GG'CG_CC
Cuts at: 2370 2370
Size: 5734
NcoI C'CATG_G
Cuts at: 513 1065 2271 513
Size: 552 1206 3976
Fragments arranged by size:
3976 1206 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5734
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5734
NotI GC'GGCC_GC
Cuts at: 3181 3181
Size: 5734
NspI r_CATG'y
Cuts at: 1132 1229 2509 3092 4637 1132
Size: 97 1280 583 1545 2229
Fragments arranged by size:
2229 1545 1280 583 97
PasI CC'CwG_GG
Cuts at: 1348 2596 1348
Size: 1248 4486
PciI A'CATG_T
Cuts at: 4633 4633
Size: 5734
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2353 1108
Size: 1245 4489
PmeI GTTT'AAAC
Cuts at: 3038 3038
Size: 5734
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3803 3835 5316 5348 3803
Size: 32 1481 32 4189
Fragments arranged by size:
4189 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2362 2410 2526 2362
Size: 48 116 5570
Fragments arranged by size:
5570 116 48
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5734
PsiI TTA'TAA
Cuts at: 3304 3304
Size: 5734
PspOMI G'GGCC_C
Cuts at: 2592 2592
Size: 5734
PstI C_TGCA'G
Cuts at: 830 3086 830
Size: 2256 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5734
SacI G_AGCT'C
Cuts at: 2553 2664 2553
Size: 111 5623
SalI G'TCGA_C
Cuts at: 1936 3076 1936
Size: 1140 4594
SanDI GG'GwC_CC
Cuts at: 2526 2526
Size: 5734
SapI GCTCTTCn'nnn_
Cuts at: 2669 2669
Size: 5734
SbfI CC_TGCA'GG
Cuts at: 3086 3086
Size: 5734
ScaI AGT'ACT
Cuts at: 3982 5694 3982
Size: 1712 4022
SfcI C'TryA_G
Cuts at: 826 1045 3082 4217 4898 5089 826
Size: 219 2037 1135 681 191 1471
Fragments arranged by size:
2037 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2371 2371
Size: 5734
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5734
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5734
SmaI CCC'GGG
Cuts at: 1872 3061 1872
Size: 1189 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2132 2414 3838 4739 5001 820
Size: 197 926 189 282 1424 901 262 1553
Fragments arranged by size:
1553 1424 926 901 282 262 197 189
SnaBI TAC'GTA
Cuts at: 493 3525 493
Size: 3032 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5734
SphI G_CATG'C
Cuts at: 1132 3092 1132
Size: 1960 3774
SspI AAT'ATT
Cuts at: 5 52 3658 5
Size: 47 3606 2081
Fragments arranged by size:
3606 2081 47
StuI AGG'CCT
Cuts at: 1517 2795 3006 1517
Size: 1278 211 4245
Fragments arranged by size:
4245 1278 211
StyI C'CwwG_G
Cuts at: 513 1065 2271 2365 3173 513
Size: 552 1206 94 808 3074
Fragments arranged by size:
3074 1206 808 552 94
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3761 3778 3931 4116 4561 325
Size: 1255 2181 17 153 185 445 1498
Fragments arranged by size:
2181 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3980 5692 96
Size: 274 80 33 51 1443 2003 1712 138
Fragments arranged by size:
2003 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5694 5726 5694
Size: 32 5702
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5734
XbaI T'CTAG_A
Cuts at: 3070 3070
Size: 5734
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2350 2350
Size: 5734
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5734
XmaI C'CCGG_G
Cuts at: 1870 3059 1870
Size: 1189 4545
XmnI GAAnn'nnTTC
Cuts at: 2777 3863 2777
Size: 1086 4648
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3542 276
Size: 53 83 186 936 2008 2468
Fragments arranged by size:
2468 2008 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI
BstZ17I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI
PacI PfoI PmlI PspXI PsrI PvuII RsrII SacII
SexAI SfiI SrfI SwaI