(Circular) (Six-base) MAPSORT of: pFN21AE0672.seq Check: 8619 from: 1 to: 5734 pFN21AE0672 5734 bp With 182 enzymes: SgfI * October 20, 2009 10:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2792 2792 Size: 5734 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3544 278 Size: 53 83 186 936 2008 2468 Fragments arranged by size: 2468 2008 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2046 3077 1937 Size: 109 1031 4594 Fragments arranged by size: 4594 1031 109 Acc65I G'GTAC_C Cuts at: 2628 3055 2628 Size: 427 5307 AclI AA'CG_TT Cuts at: 1563 3861 4234 1563 Size: 2298 373 3063 Fragments arranged by size: 3063 2298 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2036 2650 3797 5181 5449 1632 Size: 404 614 1147 1384 268 1917 Fragments arranged by size: 1917 1384 1147 614 404 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5537 AflIII A'CryG_T Cuts at: 1203 4633 5610 1203 Size: 3430 977 1327 Fragments arranged by size: 3430 1327 977 AgeI A'CCGG_T Cuts at: 4565 4565 Size: 5734 AhdI GACnn_n'nnGTC Cuts at: 4463 4463 Size: 5734 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5734 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5734 AlwNI CAG_nnn'CTG Cuts at: 2677 3477 5049 2677 Size: 800 1572 3362 Fragments arranged by size: 3362 1572 800 ApaI G_GGCC'C Cuts at: 2596 2596 Size: 5734 ApaLI G'TGCA_C Cuts at: 2402 3791 4947 2402 Size: 1389 1156 3189 Fragments arranged by size: 3189 1389 1156 ApoI r'AATT_y Cuts at: 1493 3043 3269 3421 5530 1493 Size: 1550 226 152 2109 1697 Fragments arranged by size: 2109 1697 1550 226 152 AseI AT'TA_AT Cuts at: 160 1032 4288 160 Size: 872 3256 1606 Fragments arranged by size: 3256 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5734 AvaI C'yCGr_G Cuts at: 1870 1943 2071 3059 1870 Size: 73 128 988 4545 Fragments arranged by size: 4545 988 128 73 BamHI G'GATC_C Cuts at: 3064 3431 3064 Size: 367 5367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2075 2369 2513 2628 2748 Size: 325 217 627 288 294 144 115 120 Cuts at: 2748 2802 3055 4510 618 Size: 54 253 1455 1842 Fragments arranged by size: 1842 1455 627 325 294 288 253 217 144 120 115 54 BanII G_rGCy'C Cuts at: 1391 2114 2252 2553 2596 2664 3053 1391 Size: 723 138 301 43 68 389 4072 Fragments arranged by size: 4072 723 389 301 138 68 43 BbeI G_GCGC'C Cuts at: 2373 2373 Size: 5734 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4763 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3925 3959 1903 Size: 34 50 34 1904 34 3678 Fragments arranged by size: 3678 1904 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3626 4836 3626 Size: 1210 4524 BclI T'GATC_A Cuts at: 1548 1548 Size: 5734 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3003 4345 136 Size: 107 122 71 631 605 1331 1342 1525 Fragments arranged by size: 1525 1342 1331 631 605 122 107 71 BglII A'GATC_T Cuts at: 5729 5729 Size: 5734 BlpI GC'TnA_GC Cuts at: 3151 3151 Size: 5734 Bme1580I G_kGCm'C Cuts at: 1790 2406 2596 3795 4951 1790 Size: 616 190 1199 1156 2573 Fragments arranged by size: 2573 1199 1156 616 190 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5734 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3465 4423 442 Size: 416 53 83 399 24 2048 958 1753 Fragments arranged by size: 2048 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5734 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2128 2338 2716 2986 2128 Size: 210 378 270 4876 Fragments arranged by size: 4876 378 270 210 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2252 2777 4394 1239 Size: 1013 525 1617 2579 Fragments arranged by size: 2579 1617 1013 525 Bpu10I CC'TnA_GC Cuts at: 2658 2658 Size: 5734 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2117 2399 3859 4724 5022 2117 Size: 282 1460 865 298 2829 Fragments arranged by size: 2829 1460 865 298 282 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2473 2757 4397 882 Size: 617 974 284 1640 2219 Fragments arranged by size: 2219 1640 974 617 284 BsaAI yAC'GTr Cuts at: 493 1204 3525 493 Size: 711 2321 2702 Fragments arranged by size: 2702 2321 711 BsaBI GATnn'nnATC Cuts at: 5728 5728 Size: 5734 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2370 2601 3541 3923 Size: 53 83 186 936 837 231 940 382 Cuts at: 3923 4558 275 Size: 635 1451 Fragments arranged by size: 1451 940 936 837 635 382 231 186 83 53 BsaWI w'CCGG_w Cuts at: 2868 2934 4167 4565 4839 4986 5397 2868 Size: 66 1233 398 274 147 411 3205 Fragments arranged by size: 3205 1233 411 398 274 147 66 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5509 Fragments arranged by size: 5509 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5734 BseYI C'CCAG_C Cuts at: 2498 2941 4937 5433 2498 Size: 443 1996 496 2799 Fragments arranged by size: 2799 1996 496 443 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2369 1163 Size: 573 633 4528 Fragments arranged by size: 4528 633 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3184 3945 4973 1540 Size: 275 186 1183 761 1028 2301 Fragments arranged by size: 2301 1183 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2199 2406 2553 2664 3795 3880 4951 2199 Size: 207 147 111 1131 85 1071 2982 Fragments arranged by size: 2982 1131 1071 207 147 111 85 BsmI GAATG_Cn' Cuts at: 3245 3338 3245 Size: 93 5641 BsmBI CGTCTCn'nnnn_ Cuts at: 2380 2380 Size: 5734 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2114 2199 2252 2406 2553 2596 2664 Size: 399 324 85 53 154 147 43 68 Cuts at: 2664 3053 3795 3880 4951 1391 Size: 389 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 389 324 154 147 85 85 68 53 43 BspEI T'CCGG_A Cuts at: 2868 2934 5397 2868 Size: 66 2463 3205 Fragments arranged by size: 3205 2463 66 BspHI T'CATG_A Cuts at: 3623 5353 3623 Size: 1730 4004 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2391 2792 3089 844 Size: 1547 401 297 3489 Fragments arranged by size: 3489 1547 401 297 BsrBI CCG'CTC Cuts at: 1394 1823 2107 3621 1394 Size: 429 284 1514 3507 Fragments arranged by size: 3507 1514 429 284 BsrDI GCAATG_nn' Cuts at: 65 1248 4229 4403 65 Size: 1183 2981 174 1396 Fragments arranged by size: 2981 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4378 4565 1700 Size: 110 2568 187 2869 Fragments arranged by size: 2869 2568 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5734 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5734 BssSI C'ACGA_G Cuts at: 2123 2243 3794 4806 2123 Size: 120 1551 1012 3051 Fragments arranged by size: 3051 1551 1012 120 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5734 BstBI TT'CG_AA Cuts at: 4538 4538 Size: 5734 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5734 BstXI CCAn_nnnn'nTGG Cuts at: 2278 2350 2278 Size: 72 5662 BstYI r'GATC_y Cuts at: 1972 2052 2673 2811 2865 2949 3064 3431 3816 Size: 80 621 138 54 84 115 367 385 Cuts at: 3816 3833 5285 5371 5383 5729 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 621 385 367 346 138 115 86 84 80 54 17 12 Bsu36I CC'TnA_GG Cuts at: 2678 2678 Size: 5734 BtgI C'CryG_G Cuts at: 513 1065 2271 513 Size: 552 1206 3976 Fragments arranged by size: 3976 1206 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4995 Fragments arranged by size: 4995 683 56 BtsI GCAGTG_nn' Cuts at: 2199 3250 4044 4064 2199 Size: 1051 794 20 3869 Fragments arranged by size: 3869 1051 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5734 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2428 2463 529 Size: 35 1864 35 3800 Fragments arranged by size: 3800 1864 35 35 DraI TTT'AAA Cuts at: 3038 3038 Size: 5734 DraIII CAC_nnn'GTG Cuts at: 2125 2125 Size: 5734 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4741 809 Size: 507 3425 1802 Fragments arranged by size: 3425 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2268 3016 3181 Size: 54 1425 50 275 18 438 748 165 Cuts at: 3181 4070 5699 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 748 438 275 165 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3181 1537 Size: 275 1369 4090 Fragments arranged by size: 4090 1369 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2669 2768 3664 1353 Size: 1316 99 896 3423 Fragments arranged by size: 3423 1316 896 99 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4321 4695 4841 387 Size: 1064 138 2732 374 146 1280 Fragments arranged by size: 2732 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2551 2662 2551 Size: 111 5623 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2036 2252 2650 2777 3797 4394 5181 Size: 393 404 216 398 127 1020 597 787 Cuts at: 5181 5449 1239 Size: 268 1524 Fragments arranged by size: 1524 1020 787 597 404 398 393 268 216 127 EcoNI CCTnn'n_nnAGG Cuts at: 2833 2833 Size: 5734 EcoO109I rG'GnC_Cy Cuts at: 2152 2170 2362 2410 2526 2593 2967 2152 Size: 18 192 48 116 67 374 4919 Fragments arranged by size: 4919 374 192 116 67 48 18 EcoRI G'AATT_C Cuts at: 3043 3421 3043 Size: 378 5356 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2920 2920 Size: 5734 FspI TGC'GCA Cuts at: 4240 4240 Size: 5734 HaeII r_GCGC'y Cuts at: 1678 2373 4881 1678 Size: 695 2508 2531 Fragments arranged by size: 2531 2508 695 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2705 2737 4375 4407 4449 Size: 32 135 32 1133 32 1638 32 42 Cuts at: 4449 4481 1373 Size: 32 2626 Fragments arranged by size: 2626 1638 1133 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3078 3324 669 Size: 988 281 1140 246 3079 Fragments arranged by size: 3079 1140 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5734 HpaI GTT'AAC Cuts at: 3324 3324 Size: 5734 KasI G'GCGC_C Cuts at: 2369 2369 Size: 5734 KpnI G_GTAC'C Cuts at: 2632 3059 2632 Size: 427 5307 MfeI C'AATT_G Cuts at: 3333 3333 Size: 5734 MluI A'CGCG_T Cuts at: 5610 5610 Size: 5734 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4848 5032 1305 Size: 3543 184 2007 Fragments arranged by size: 3543 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2270 3018 10 Size: 54 1425 343 438 748 2726 Fragments arranged by size: 2726 1425 748 438 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3692 4051 4210 518 Size: 648 2526 359 159 2042 Fragments arranged by size: 2526 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3150 3827 4975 5220 1606 Size: 1544 677 1148 245 2120 Fragments arranged by size: 2120 1544 1148 677 245 NarI GG'CG_CC Cuts at: 2370 2370 Size: 5734 NcoI C'CATG_G Cuts at: 513 1065 2271 513 Size: 552 1206 3976 Fragments arranged by size: 3976 1206 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5734 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5734 NotI GC'GGCC_GC Cuts at: 3181 3181 Size: 5734 NspI r_CATG'y Cuts at: 1132 1229 2509 3092 4637 1132 Size: 97 1280 583 1545 2229 Fragments arranged by size: 2229 1545 1280 583 97 PasI CC'CwG_GG Cuts at: 1348 2596 1348 Size: 1248 4486 PciI A'CATG_T Cuts at: 4633 4633 Size: 5734 PflMI CCAn_nnn'nTGG Cuts at: 1108 2353 1108 Size: 1245 4489 PmeI GTTT'AAAC Cuts at: 3038 3038 Size: 5734 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3803 3835 5316 5348 3803 Size: 32 1481 32 4189 Fragments arranged by size: 4189 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2362 2410 2526 2362 Size: 48 116 5570 Fragments arranged by size: 5570 116 48 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5734 PsiI TTA'TAA Cuts at: 3304 3304 Size: 5734 PspOMI G'GGCC_C Cuts at: 2592 2592 Size: 5734 PstI C_TGCA'G Cuts at: 830 3086 830 Size: 2256 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5734 SacI G_AGCT'C Cuts at: 2553 2664 2553 Size: 111 5623 SalI G'TCGA_C Cuts at: 1936 3076 1936 Size: 1140 4594 SanDI GG'GwC_CC Cuts at: 2526 2526 Size: 5734 SapI GCTCTTCn'nnn_ Cuts at: 2669 2669 Size: 5734 SbfI CC_TGCA'GG Cuts at: 3086 3086 Size: 5734 ScaI AGT'ACT Cuts at: 3982 5694 3982 Size: 1712 4022 SfcI C'TryA_G Cuts at: 826 1045 3082 4217 4898 5089 826 Size: 219 2037 1135 681 191 1471 Fragments arranged by size: 2037 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2371 2371 Size: 5734 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5734 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5734 SmaI CCC'GGG Cuts at: 1872 3061 1872 Size: 1189 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2132 2414 3838 4739 5001 820 Size: 197 926 189 282 1424 901 262 1553 Fragments arranged by size: 1553 1424 926 901 282 262 197 189 SnaBI TAC'GTA Cuts at: 493 3525 493 Size: 3032 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5734 SphI G_CATG'C Cuts at: 1132 3092 1132 Size: 1960 3774 SspI AAT'ATT Cuts at: 5 52 3658 5 Size: 47 3606 2081 Fragments arranged by size: 3606 2081 47 StuI AGG'CCT Cuts at: 1517 2795 3006 1517 Size: 1278 211 4245 Fragments arranged by size: 4245 1278 211 StyI C'CwwG_G Cuts at: 513 1065 2271 2365 3173 513 Size: 552 1206 94 808 3074 Fragments arranged by size: 3074 1206 808 552 94 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3761 3778 3931 4116 4561 325 Size: 1255 2181 17 153 185 445 1498 Fragments arranged by size: 2181 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3980 5692 96 Size: 274 80 33 51 1443 2003 1712 138 Fragments arranged by size: 2003 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5694 5726 5694 Size: 32 5702 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5734 XbaI T'CTAG_A Cuts at: 3070 3070 Size: 5734 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2350 2350 Size: 5734 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5734 XmaI C'CCGG_G Cuts at: 1870 3059 1870 Size: 1189 4545 XmnI GAAnn'nnTTC Cuts at: 2777 3863 2777 Size: 1086 4648 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3542 276 Size: 53 83 186 936 2008 2468 Fragments arranged by size: 2468 2008 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacII SexAI SfiI SrfI SwaI