Restriction map for
pFN21AE0671
(Circular) (Six-base) MAPSORT of: pFN21AE0671.seq Check: 1956 from: 1 to: 5479
pFN21AE0671 5479 bp
With 182 enzymes: SgfI *
October 20, 2009 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2298 2298
Size: 5479
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3289 278
Size: 53 83 186 936 1753 2468
Fragments arranged by size:
2468 1753 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2822 1937
Size: 885 4594
Acc65I G'GTAC_C
Cuts at: 2150 2800 2150
Size: 650 4829
AclI AA'CG_TT
Cuts at: 1563 3606 3979 1563
Size: 2043 373 3063
Fragments arranged by size:
3063 2043 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3542 4926 5194 1632
Size: 1910 1384 268 1917
Fragments arranged by size:
1917 1910 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5282
AflIII A'CryG_T
Cuts at: 1203 4378 5355 1203
Size: 3175 977 1327
Fragments arranged by size:
3175 1327 977
AgeI A'CCGG_T
Cuts at: 4310 4310
Size: 5479
AhdI GACnn_n'nnGTC
Cuts at: 4208 4208
Size: 5479
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5479
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5479
AlwNI CAG_nnn'CTG
Cuts at: 2615 3222 4794 2615
Size: 607 1572 3300
Fragments arranged by size:
3300 1572 607
ApaI G_GGCC'C
Cuts at: 2077 2077
Size: 5479
ApaLI G'TGCA_C
Cuts at: 3536 4692 3536
Size: 1156 4323
ApoI r'AATT_y
Cuts at: 1493 2788 3014 3166 5275 1493
Size: 1295 226 152 2109 1697
Fragments arranged by size:
2109 1697 1295 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4033 160
Size: 872 3001 1606
Fragments arranged by size:
3001 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5479
AvaI C'yCGr_G
Cuts at: 1870 1943 2132 2804 1870
Size: 73 189 672 4545
Fragments arranged by size:
4545 672 189 73
BamHI G'GATC_C
Cuts at: 2809 3176 2809
Size: 367 5112
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2150 2800 4255 618
Size: 325 217 627 363 650 1455 1842
Fragments arranged by size:
1842 1455 650 627 363 325 217
BanII G_rGCy'C
Cuts at: 1391 2077 2798 1391
Size: 686 721 4072
Fragments arranged by size:
4072 721 686
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4508
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3670 3704 1903
Size: 34 1733 34 3678
Fragments arranged by size:
3678 1733 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3371 4581 3371
Size: 1210 4269
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5479
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2276 4090 136
Size: 107 122 71 631 605 604 1814 1525
Fragments arranged by size:
1814 1525 631 605 604 122 107 71
BglII A'GATC_T
Cuts at: 5474 5474
Size: 5479
BlpI GC'TnA_GC
Cuts at: 2896 2896
Size: 5479
Bme1580I G_kGCm'C
Cuts at: 1790 2077 2665 3540 4696 1790
Size: 287 588 875 1156 2573
Fragments arranged by size:
2573 1156 875 588 287
BmgBI CAC'GTC
Cuts at: 1318 2716 1318
Size: 1398 4081
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2064 3210 4168
Size: 416 53 83 399 24 647 1146 958
Cuts at: 4168 442
Size: 1753
Fragments arranged by size:
1753 1146 958 647 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5479
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2395 4139 1239
Size: 1156 1744 2579
Fragments arranged by size:
2579 1744 1156
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3604 4469 4767 3604
Size: 865 298 4316
Fragments arranged by size:
4316 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4142 882
Size: 617 2643 2219
Fragments arranged by size:
2643 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2324 3270 493
Size: 711 1120 946 2702
Fragments arranged by size:
2702 1120 946 711
BsaBI GATnn'nnATC
Cuts at: 5473 5473
Size: 5479
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3286 3668 4303 275
Size: 53 83 186 936 1753 382 635 1451
Fragments arranged by size:
1753 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2464 3912 4310 4584 4731 5142 2464
Size: 1448 398 274 147 411 2801
Fragments arranged by size:
2801 1448 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5254
Fragments arranged by size:
5254 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2150 2210 2592 1186
Size: 964 60 382 4073
Fragments arranged by size:
4073 964 382 60
BseYI C'CCAG_C
Cuts at: 2120 2155 2339 2587 4682 5178 2120
Size: 35 184 248 2095 496 2421
Fragments arranged by size:
2421 2095 496 248 184 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2488 2504 2657 1163
Size: 573 752 16 153 3985
Fragments arranged by size:
3985 752 573 153 16
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2929 3690 4718 1540
Size: 275 186 928 761 1028 2301
Fragments arranged by size:
2301 1028 928 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3540 3625 4696 3540
Size: 85 1071 4323
Fragments arranged by size:
4323 1071 85
BsmI GAATG_Cn'
Cuts at: 2990 3083 2990
Size: 93 5386
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2077 2665 2798 3540 3625 4696 1391
Size: 399 287 588 133 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 588 399 287 133 85
BspEI T'CCGG_A
Cuts at: 2464 5142 2464
Size: 2678 2801
BspHI T'CATG_A
Cuts at: 3368 5098 3368
Size: 1730 3749
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2298 2834 844
Size: 1454 536 3489
Fragments arranged by size:
3489 1454 536
BsrBI CCG'CTC
Cuts at: 1394 1823 2050 3366 1394
Size: 429 227 1316 3507
Fragments arranged by size:
3507 1316 429 227
BsrDI GCAATG_nn'
Cuts at: 65 1248 3974 4148 65
Size: 1183 2726 174 1396
Fragments arranged by size:
2726 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4123 4310 1700
Size: 110 2313 187 2869
Fragments arranged by size:
2869 2313 187 110
BsrGI T'GTAC_A
Cuts at: 96 2620 96
Size: 2524 2955
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5479
BssSI C'ACGA_G
Cuts at: 2373 3539 4551 2373
Size: 1166 1012 3301
Fragments arranged by size:
3301 1166 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2113 2299 2597 794
Size: 1319 186 298 3676
Fragments arranged by size:
3676 1319 298 186
BstBI TT'CG_AA
Cuts at: 4283 4283
Size: 5479
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5479
BstYI r'GATC_y
Cuts at: 1972 2200 2809 3176 3561 3578 5030 5116 5128
Size: 228 609 367 385 17 1452 86 12
Cuts at: 5128 5474 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 609 385 367 346 228 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2565 2565
Size: 5479
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3988
Fragments arranged by size:
3988 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4740
Fragments arranged by size:
4740 683 56
BtsI GCAGTG_nn'
Cuts at: 2505 2591 2613 2665 2729 2995 3789 3809 2505
Size: 86 22 52 64 266 794 20 4175
Fragments arranged by size:
4175 794 266 86 64 52 22 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5479
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5444
DraI TTT'AAA
Cuts at: 2783 2783
Size: 5479
DraIII CAC_nnn'GTG
Cuts at: 2494 2494
Size: 5479
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4486 809
Size: 507 3170 1802
Fragments arranged by size:
3170 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2268 2709 2745
Size: 54 1425 50 275 18 438 441 36
Cuts at: 2745 2926 3815 5444 8
Size: 181 889 1629 43
Fragments arranged by size:
1629 1425 889 441 438 275 181 54
50 43 36 18
EagI C'GGCC_G
Cuts at: 1537 1812 2926 1537
Size: 275 1114 4090
Fragments arranged by size:
4090 1114 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3409 1353
Size: 2056 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4066 4440 4586 387
Size: 1064 138 2477 374 146 1280
Fragments arranged by size:
2477 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2395 3542 4139 4926 5194 1239
Size: 393 763 1147 597 787 268 1524
Fragments arranged by size:
1524 1147 787 763 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2135 2135
Size: 5479
EcoO109I rG'GnC_Cy
Cuts at: 2024 2074 2124 2024
Size: 50 50 5379
Fragments arranged by size:
5379 50 50
EcoRI G'AATT_C
Cuts at: 2788 3166 2788
Size: 378 5101
FspI TGC'GCA
Cuts at: 3985 3985
Size: 5479
HaeII r_GCGC'y
Cuts at: 1678 4626 1678
Size: 2948 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4120 4152 4194 4226 1373
Size: 32 135 32 2548 32 42 32 2626
Fragments arranged by size:
2626 2548 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2823 3069 669
Size: 988 281 885 246 3079
Fragments arranged by size:
3079 988 885 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5479
HpaI GTT'AAC
Cuts at: 3069 3069
Size: 5479
KpnI G_GTAC'C
Cuts at: 2154 2804 2154
Size: 650 4829
MfeI C'AATT_G
Cuts at: 3078 3078
Size: 5479
MluI A'CGCG_T
Cuts at: 5355 5355
Size: 5479
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2454 2782 4593 4777 1305
Size: 1149 328 1811 184 2007
Fragments arranged by size:
2007 1811 1149 328 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3657
Fragments arranged by size:
3657 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3437 3796 3955 518
Size: 648 2271 359 159 2042
Fragments arranged by size:
2271 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2062 2895 3572 4720 4965 1606
Size: 456 833 677 1148 245 2120
Fragments arranged by size:
2120 1148 833 677 456 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3988
Fragments arranged by size:
3988 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5479
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5479
NotI GC'GGCC_GC
Cuts at: 2926 2926
Size: 5479
NspI r_CATG'y
Cuts at: 1132 1229 2837 4382 1132
Size: 97 1608 1545 2229
Fragments arranged by size:
2229 1608 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5479
PciI A'CATG_T
Cuts at: 4378 4378
Size: 5479
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5479
PmeI GTTT'AAAC
Cuts at: 2783 2783
Size: 5479
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3548 3580 5061 5093 3548
Size: 32 1481 32 3934
Fragments arranged by size:
3934 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2024 2024
Size: 5479
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5479
PsiI TTA'TAA
Cuts at: 3049 3049
Size: 5479
PspOMI G'GGCC_C
Cuts at: 2073 2073
Size: 5479
PspXI vC'TCGA_Gb
Cuts at: 2132 2132
Size: 5479
PstI C_TGCA'G
Cuts at: 830 2295 2831 830
Size: 1465 536 3478
Fragments arranged by size:
3478 1465 536
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5479
SalI G'TCGA_C
Cuts at: 1936 2821 1936
Size: 885 4594
SbfI CC_TGCA'GG
Cuts at: 2831 2831
Size: 5479
ScaI AGT'ACT
Cuts at: 3727 5439 3727
Size: 1712 3767
SfcI C'TryA_G
Cuts at: 826 1045 2291 2827 3962 4643 4834 826
Size: 219 1246 536 1135 681 191 1471
Fragments arranged by size:
1471 1246 1135 681 536 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2276 2276
Size: 5479
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5479
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5479
SmaI CCC'GGG
Cuts at: 1872 2806 1872
Size: 934 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2132 3583 4484 4746 820
Size: 197 926 189 1451 901 262 1553
Fragments arranged by size:
1553 1451 926 901 262 197 189
SnaBI TAC'GTA
Cuts at: 493 3270 493
Size: 2777 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5479
SphI G_CATG'C
Cuts at: 1132 2837 1132
Size: 1705 3774
SspI AAT'ATT
Cuts at: 5 52 3403 5
Size: 47 3351 2081
Fragments arranged by size:
3351 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5479
StyI C'CwwG_G
Cuts at: 513 1065 2004 2918 513
Size: 552 939 914 3074
Fragments arranged by size:
3074 939 914 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3506 3523 3676 3861 4306 325
Size: 1255 1926 17 153 185 445 1498
Fragments arranged by size:
1926 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2620 3725 5437
Size: 274 80 33 51 1443 643 1105 1712
Cuts at: 5437 96
Size: 138
Fragments arranged by size:
1712 1443 1105 643 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2192 2224 5439 5471 2192
Size: 32 3215 32 2200
Fragments arranged by size:
3215 2200 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2028 1316
Size: 712 4767
XbaI T'CTAG_A
Cuts at: 2815 2815
Size: 5479
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2419 2419
Size: 5479
XhoI C'TCGA_G
Cuts at: 1943 2132 1943
Size: 189 5290
XmaI C'CCGG_G
Cuts at: 1870 2804 1870
Size: 934 4545
XmnI GAAnn'nnTTC
Cuts at: 3608 3608
Size: 5479
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3287 276
Size: 53 83 186 936 1753 2468
Fragments arranged by size:
2468 1753 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI SalI SbfI ScaI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfoI SrfI SwaI