Restriction map for
pFN21AE0668
(Circular) (Six-base) MAPSORT of: pFN21AE0668.seq Check: 1429 from: 1 to: 5884
pFN21AE0668 5884 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3694 278
Size: 53 83 186 936 2158 2468
Fragments arranged by size:
2468 2158 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3227 1937
Size: 1290 4594
Acc65I G'GTAC_C
Cuts at: 3205 3205
Size: 5884
AclI AA'CG_TT
Cuts at: 1563 4011 4384 1563
Size: 2448 373 3063
Fragments arranged by size:
3063 2448 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2213 2489 3947 5331 5599 1632
Size: 581 276 1458 1384 268 1917
Fragments arranged by size:
1917 1458 1384 581 276 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5687
AflIII A'CryG_T
Cuts at: 1203 2691 4783 5760 1203
Size: 1488 2092 977 1327
Fragments arranged by size:
2092 1488 1327 977
AgeI A'CCGG_T
Cuts at: 4715 4715
Size: 5884
AhdI GACnn_n'nnGTC
Cuts at: 4613 4613
Size: 5884
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5884
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5884
AlwNI CAG_nnn'CTG
Cuts at: 2356 2427 3627 5199 2356
Size: 71 1200 1572 3041
Fragments arranged by size:
3041 1572 1200 71
ApaLI G'TGCA_C
Cuts at: 3941 5097 3941
Size: 1156 4728
ApoI r'AATT_y
Cuts at: 1493 2484 2496 3002 3193 3419 3571 5680 1493
Size: 991 12 506 191 226 152 2109 1697
Fragments arranged by size:
2109 1697 991 506 226 191 152 12
AseI AT'TA_AT
Cuts at: 160 1032 4438 160
Size: 872 3406 1606
Fragments arranged by size:
3406 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5884
AvaI C'yCGr_G
Cuts at: 1870 1943 2882 3209 1870
Size: 73 939 327 4545
Fragments arranged by size:
4545 939 327 73
BamHI G'GATC_C
Cuts at: 3214 3581 3214
Size: 367 5517
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2832 3205 4660 618
Size: 325 217 627 1045 373 1455 1842
Fragments arranged by size:
1842 1455 1045 627 373 325 217
BanII G_rGCy'C
Cuts at: 1391 3203 1391
Size: 1812 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4913
BbvCI CC'TCA_GC
Cuts at: 3102 3102
Size: 5884
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2039 2073 4075 4109 1903
Size: 34 50 34 18 34 2002 34 3678
Fragments arranged by size:
3678 2002 50 34 34 34 34 18
BciVI GTATCCnnnnn_n'
Cuts at: 2796 3776 4986 2796
Size: 980 1210 3694
Fragments arranged by size:
3694 1210 980
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5884
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4495 136
Size: 107 122 71 631 605 2823 1525
Fragments arranged by size:
2823 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3092 5879 3092
Size: 2787 3097
BlpI GC'TnA_GC
Cuts at: 3301 3301
Size: 5884
Bme1580I G_kGCm'C
Cuts at: 1790 2681 3945 5101 1790
Size: 891 1264 1156 2573
Fragments arranged by size:
2573 1264 1156 891
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5884
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2976 3615 4573
Size: 416 53 83 399 24 1559 639 958
Cuts at: 4573 442
Size: 1753
Fragments arranged by size:
1753 1559 958 639 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5884
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2386 2386
Size: 5884
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2135 2327 2693 2764 2784 2837 4544 1239
Size: 896 192 366 71 20 53 1707 2579
Fragments arranged by size:
2579 1707 896 366 192 71 53 20
Bpu10I CC'TnA_GC
Cuts at: 2894 3102 2894
Size: 208 5676
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2174 4009 4874 5172 2174
Size: 1835 865 298 2886
Fragments arranged by size:
2886 1835 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2078 2263 2852 4547 882
Size: 617 579 185 589 1695 2219
Fragments arranged by size:
2219 1695 617 589 579 185
BsaAI yAC'GTr
Cuts at: 493 1204 3675 493
Size: 711 2471 2702
Fragments arranged by size:
2702 2471 711
BsaBI GATnn'nnATC
Cuts at: 2423 5878 2423
Size: 3455 2429
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2024 3180 3691 4073
Size: 53 83 186 936 491 1156 511 382
Cuts at: 4073 4708 275
Size: 635 1451
Fragments arranged by size:
1451 1156 936 635 511 491 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2325 4317 4715 4989 5136 5547 2325
Size: 1992 398 274 147 411 2662
Fragments arranged by size:
2662 1992 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2810 2840 1178
Size: 30 165 30 1407 30 4222
Fragments arranged by size:
4222 1407 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2132 2787 2958 1186
Size: 946 655 171 4112
Fragments arranged by size:
4112 946 655 171
BseYI C'CCAG_C
Cuts at: 2224 3155 5087 5583 2224
Size: 931 1932 496 2525
Fragments arranged by size:
2525 1932 931 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2750 1163
Size: 573 1014 4297
Fragments arranged by size:
4297 1014 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3334 4095 5123 1540
Size: 275 186 1333 761 1028 2301
Fragments arranged by size:
2301 1333 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2439 3945 4030 5101 2439
Size: 1506 85 1071 3222
Fragments arranged by size:
3222 1506 1071 85
BsmI GAATG_Cn'
Cuts at: 3395 3488 3395
Size: 93 5791
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2439 2681 3203 3945 4030 5101 1391
Size: 399 649 242 522 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 649 522 399 242 85
BspEI T'CCGG_A
Cuts at: 2325 5547 2325
Size: 3222 2662
BspHI T'CATG_A
Cuts at: 3773 5503 3773
Size: 1730 4154
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2341 3110 3239 844
Size: 1497 769 129 3489
Fragments arranged by size:
3489 1497 769 129
BsrBI CCG'CTC
Cuts at: 1394 1823 3771 1394
Size: 429 1948 3507
Fragments arranged by size:
3507 1948 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2242 2514 4379 4553 65
Size: 1183 994 272 1865 174 1396
Fragments arranged by size:
1865 1396 1183 994 272 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4528 4715 1700
Size: 110 2718 187 2869
Fragments arranged by size:
2869 2718 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5884
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5884
BssSI C'ACGA_G
Cuts at: 3944 4956 3944
Size: 1012 4872
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2356 794
Size: 1562 4322
BstBI TT'CG_AA
Cuts at: 4688 4688
Size: 5884
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5884
BstXI CCAn_nnnn'nTGG
Cuts at: 2815 3176 2815
Size: 361 5523
BstYI r'GATC_y
Cuts at: 1972 3092 3214 3581 3966 3983 5435 5521 5533
Size: 1120 122 367 385 17 1452 86 12
Cuts at: 5533 5879 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1120 385 367 346 122 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2605 2658 2925 513
Size: 552 1540 53 267 3472
Fragments arranged by size:
3472 1540 552 267 53
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2424 487
Size: 683 56 1198 3947
Fragments arranged by size:
3947 1198 683 56
BtsI GCAGTG_nn'
Cuts at: 3400 4194 4214 3400
Size: 794 20 5070
Fragments arranged by size:
5070 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5884
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2587 2622 529
Size: 35 2023 35 3791
Fragments arranged by size:
3791 2023 35 35
DraI TTT'AAA
Cuts at: 3188 3188
Size: 5884
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2633 4891 809
Size: 507 1317 2258 1802
Fragments arranged by size:
2258 1802 1317 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2286 2661 3331
Size: 54 1425 50 275 18 456 375 670
Cuts at: 3331 4220 5849 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 670 456 375 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3331 1537
Size: 275 1519 4090
Fragments arranged by size:
4090 1519 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3814 1353
Size: 2461 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4471 4845 4991 387
Size: 1064 138 2882 374 146 1280
Fragments arranged by size:
2882 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2135 2213 2327 2489 2693 2764 2784
Size: 393 503 78 114 162 204 71 20
Cuts at: 2784 2837 3947 4544 5331 5599 1239
Size: 53 1110 597 787 268 1524
Fragments arranged by size:
1524 1110 787 597 503 393 268 204
162 114 78 71 53 20
EcoO109I rG'GnC_Cy
Cuts at: 2685 2877 2889 2685
Size: 192 12 5680
Fragments arranged by size:
5680 192 12
EcoRI G'AATT_C
Cuts at: 2484 3193 3571 2484
Size: 709 378 4797
Fragments arranged by size:
4797 709 378
FspI TGC'GCA
Cuts at: 4390 4390
Size: 5884
HaeII r_GCGC'y
Cuts at: 1678 2152 5031 1678
Size: 474 2879 2531
Fragments arranged by size:
2879 2531 474
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4525 4557 4599 4631 1373
Size: 32 135 32 2953 32 42 32 2626
Fragments arranged by size:
2953 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2065 2599 2959 3228 3474 669
Size: 988 281 127 534 360 269 246 3079
Fragments arranged by size:
3079 988 534 360 281 269 246 127
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5884
HpaI GTT'AAC
Cuts at: 2065 3474 2065
Size: 1409 4475
KpnI G_GTAC'C
Cuts at: 3209 3209
Size: 5884
MfeI C'AATT_G
Cuts at: 3483 3483
Size: 5884
MluI A'CGCG_T
Cuts at: 5760 5760
Size: 5884
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2610 2925 4998 5182 1305
Size: 1305 315 2073 184 2007
Fragments arranged by size:
2073 2007 1305 315 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2288 2663 10
Size: 54 1425 343 456 375 3231
Fragments arranged by size:
3231 1425 456 375 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2442 2571 3842 4201 4360 518
Size: 648 1276 129 1271 359 159 2042
Fragments arranged by size:
2042 1276 1271 648 359 159 129
MspA1I CmG'CkG
Cuts at: 1606 2035 2640 3300 3977 5125 5370 1606
Size: 429 605 660 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 660 605 429 245
NcoI C'CATG_G
Cuts at: 513 1065 2658 2925 513
Size: 552 1593 267 3472
Fragments arranged by size:
3472 1593 552 267
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5884
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5884
NotI GC'GGCC_GC
Cuts at: 3331 3331
Size: 5884
NspI r_CATG'y
Cuts at: 1132 1229 2695 3242 4787 1132
Size: 97 1466 547 1545 2229
Fragments arranged by size:
2229 1545 1466 547 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5884
PciI A'CATG_T
Cuts at: 2691 4783 2691
Size: 2092 3792
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5884
PmeI GTTT'AAAC
Cuts at: 3188 3188
Size: 5884
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3953 3985 5466 5498 3953
Size: 32 1481 32 4339
Fragments arranged by size:
4339 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2685 2877 2685
Size: 192 5692
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5884
PsiI TTA'TAA
Cuts at: 3454 3454
Size: 5884
PstI C_TGCA'G
Cuts at: 830 2062 2352 3236 830
Size: 1232 290 884 3478
Fragments arranged by size:
3478 1232 884 290
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5884
PvuII CAG'CTG
Cuts at: 2035 2035
Size: 5884
SalI G'TCGA_C
Cuts at: 1936 3226 1936
Size: 1290 4594
SbfI CC_TGCA'GG
Cuts at: 3236 3236
Size: 5884
ScaI AGT'ACT
Cuts at: 4132 5844 4132
Size: 1712 4172
SfcI C'TryA_G
Cuts at: 826 1045 2058 2348 2404 3232 4367 5048 5239
Size: 219 1013 290 56 828 1135 681 191
Cuts at: 5239 826
Size: 1471
Fragments arranged by size:
1471 1135 1013 828 681 290 219 191
56
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5884
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5884
SmaI CCC'GGG
Cuts at: 1872 2884 3211 1872
Size: 1012 327 4545
Fragments arranged by size:
4545 1012 327
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2153 3988 4889 5151 820
Size: 197 926 210 1835 901 262 1553
Fragments arranged by size:
1835 1553 926 901 262 210 197
SnaBI TAC'GTA
Cuts at: 493 3675 493
Size: 3182 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5884
SphI G_CATG'C
Cuts at: 1132 3242 1132
Size: 2110 3774
SspI AAT'ATT
Cuts at: 5 52 3808 5
Size: 47 3756 2081
Fragments arranged by size:
3756 2081 47
StuI AGG'CCT
Cuts at: 1517 2099 2159 2532 1517
Size: 582 60 373 4869
Fragments arranged by size:
4869 582 373 60
StyI C'CwwG_G
Cuts at: 513 1065 2658 2808 2873 2925 3323 513
Size: 552 1593 150 65 52 398 3074
Fragments arranged by size:
3074 1593 552 398 150 65 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2820 2881 3911 3928 4081 4266 4711
Size: 1255 1240 61 1030 17 153 185 445
Cuts at: 4711 325
Size: 1498
Fragments arranged by size:
1498 1255 1240 1030 445 185 153 61
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4130 5842 96
Size: 274 80 33 51 1443 2153 1712 138
Fragments arranged by size:
2153 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5844 5876 5844
Size: 32 5852
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5884
XbaI T'CTAG_A
Cuts at: 3220 3220
Size: 5884
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2270 2671 2270
Size: 401 5483
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5884
XmaI C'CCGG_G
Cuts at: 1870 2882 3209 1870
Size: 1012 327 4545
Fragments arranged by size:
4545 1012 327
XmnI GAAnn'nnTTC
Cuts at: 4013 4013
Size: 5884
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3692 276
Size: 53 83 186 936 2158 2468
Fragments arranged by size:
2468 2158 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI