Restriction map for
pFN21AE0667
(Circular) (Six-base) MAPSORT of: pFN21AE0667.seq Check: 3974 from: 1 to: 5434
pFN21AE0667 5434 bp
With 182 enzymes: SgfI *
October 20, 2009 10:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3244 278
Size: 53 83 186 936 1708 2468
Fragments arranged by size:
2468 1708 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2777 1937
Size: 840 4594
Acc65I G'GTAC_C
Cuts at: 2755 2755
Size: 5434
AclI AA'CG_TT
Cuts at: 1563 2733 3561 3934 1563
Size: 1170 828 373 3063
Fragments arranged by size:
3063 1170 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2085 2299 3497 4881 5149 1632
Size: 453 214 1198 1384 268 1917
Fragments arranged by size:
1917 1384 1198 453 268 214
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5237
AflIII A'CryG_T
Cuts at: 1203 2056 2299 2631 4333 5310 1203
Size: 853 243 332 1702 977 1327
Fragments arranged by size:
1702 1327 977 853 332 243
AgeI A'CCGG_T
Cuts at: 4265 4265
Size: 5434
AhdI GACnn_n'nnGTC
Cuts at: 4163 4163
Size: 5434
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5434
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5434
AlwNI CAG_nnn'CTG
Cuts at: 2106 2603 3177 4749 2106
Size: 497 574 1572 2791
Fragments arranged by size:
2791 1572 574 497
ApaLI G'TGCA_C
Cuts at: 2627 3491 4647 2627
Size: 864 1156 3414
Fragments arranged by size:
3414 1156 864
ApoI r'AATT_y
Cuts at: 1493 2234 2743 2969 3121 5230 1493
Size: 741 509 226 152 2109 1697
Fragments arranged by size:
2109 1697 741 509 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3988 160
Size: 872 2956 1606
Fragments arranged by size:
2956 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5434
AvaI C'yCGr_G
Cuts at: 1870 1943 2337 2759 1870
Size: 73 394 422 4545
Fragments arranged by size:
4545 422 394 73
BamHI G'GATC_C
Cuts at: 2764 3131 2764
Size: 367 5067
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2755 4210 618
Size: 325 217 627 968 1455 1842
Fragments arranged by size:
1842 1455 968 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2042 2592 2753 1391
Size: 651 550 161 4072
Fragments arranged by size:
4072 651 550 161
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4463
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3625 3659 1903
Size: 34 1688 34 3678
Fragments arranged by size:
3678 1688 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3326 4536 3326
Size: 1210 4224
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5434
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4045 136
Size: 107 122 71 631 605 2373 1525
Fragments arranged by size:
2373 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5429 5429
Size: 5434
BlpI GC'TnA_GC
Cuts at: 2851 2851
Size: 5434
Bme1580I G_kGCm'C
Cuts at: 1790 2631 3495 4651 1790
Size: 841 864 1156 2573
Fragments arranged by size:
2573 1156 864 841
BmgBI CAC'GTC
Cuts at: 1318 2059 2300 1318
Size: 741 241 4452
Fragments arranged by size:
4452 741 241
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2116 3165 4123
Size: 416 53 83 399 24 699 1049 958
Cuts at: 4123 442
Size: 1753
Fragments arranged by size:
1753 1049 958 699 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5434
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2397 4094 1239
Size: 1158 1697 2579
Fragments arranged by size:
2579 1697 1158
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3559 4424 4722 3559
Size: 865 298 4271
Fragments arranged by size:
4271 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4097 882
Size: 617 2598 2219
Fragments arranged by size:
2598 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2632 3225 493
Size: 711 1428 593 2702
Fragments arranged by size:
2702 1428 711 593
BsaBI GATnn'nnATC
Cuts at: 5428 5428
Size: 5434
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2704 3241 3623 4258
Size: 53 83 186 936 1171 537 382 635
Cuts at: 4258 275
Size: 1451
Fragments arranged by size:
1451 1171 936 635 537 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3867 4265 4539 4686 5097 3867
Size: 398 274 147 411 4204
Fragments arranged by size:
4204 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5209
Fragments arranged by size:
5209 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2050 2537 1186
Size: 864 487 4083
Fragments arranged by size:
4083 864 487
BseYI C'CCAG_C
Cuts at: 2451 4637 5133 2451
Size: 2186 496 2752
Fragments arranged by size:
2752 2186 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2420 1163
Size: 573 684 4177
Fragments arranged by size:
4177 684 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2884 3645 4673 1540
Size: 275 186 883 761 1028 2301
Fragments arranged by size:
2301 1028 883 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2592 2631 3495 3580 4651 2592
Size: 39 864 85 1071 3375
Fragments arranged by size:
3375 1071 864 85 39
BsmI GAATG_Cn'
Cuts at: 2945 3038 2945
Size: 93 5341
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2042 2592 2631 2753 3495 3580 4651
Size: 399 252 550 39 122 742 85 1071
Cuts at: 4651 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 550 399 252 122 85
39
BspEI T'CCGG_A
Cuts at: 5097 5097
Size: 5434
BspHI T'CATG_A
Cuts at: 2356 3323 5053 2356
Size: 967 1730 2737
Fragments arranged by size:
2737 1730 967
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2789 844
Size: 1945 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3321 1394
Size: 429 1498 3507
Fragments arranged by size:
3507 1498 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2197 3929 4103 65
Size: 1183 949 1732 174 1396
Fragments arranged by size:
1732 1396 1183 949 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4078 4265 1700
Size: 110 2268 187 2869
Fragments arranged by size:
2869 2268 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5434
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5434
BssSI C'ACGA_G
Cuts at: 3494 4506 3494
Size: 1012 4422
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5434
BstBI TT'CG_AA
Cuts at: 4238 4238
Size: 5434
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5434
BstYI r'GATC_y
Cuts at: 1972 2764 3131 3516 3533 4985 5071 5083 5429
Size: 792 367 385 17 1452 86 12 346
Cuts at: 5429 1972
Size: 1977
Fragments arranged by size:
1977 1452 792 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4882
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4695
Fragments arranged by size:
4695 683 56
BtsI GCAGTG_nn'
Cuts at: 2950 3744 3764 2950
Size: 794 20 4620
Fragments arranged by size:
4620 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5434
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5399
DraI TTT'AAA
Cuts at: 2738 2738
Size: 5434
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2111 4441 809
Size: 507 795 2330 1802
Fragments arranged by size:
2330 1802 795 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2559 2881 3770
Size: 54 1425 50 275 18 729 322 889
Cuts at: 3770 5399 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 729 322 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2881 1537
Size: 275 1069 4090
Fragments arranged by size:
4090 1069 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2214 2652 3364 1353
Size: 861 438 712 3423
Fragments arranged by size:
3423 861 712 438
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4021 4395 4541 387
Size: 1064 138 2432 374 146 1280
Fragments arranged by size:
2432 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2590 2590
Size: 5434
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2085 2299 2397 3497 4094 4881 5149
Size: 393 453 214 98 1100 597 787 268
Cuts at: 5149 1239
Size: 1524
Fragments arranged by size:
1524 1100 787 597 453 393 268 214
98
EcoO109I rG'GnC_Cy
Cuts at: 2662 2662
Size: 5434
EcoRI G'AATT_C
Cuts at: 2743 3121 2743
Size: 378 5056
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2410 2410
Size: 5434
FspI TGC'GCA
Cuts at: 3940 3940
Size: 5434
HaeII r_GCGC'y
Cuts at: 1678 4581 1678
Size: 2903 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4075 4107 4149 4181 1373
Size: 32 135 32 2503 32 42 32 2626
Fragments arranged by size:
2626 2503 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2778 3024 669
Size: 988 281 840 246 3079
Fragments arranged by size:
3079 988 840 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5434
HpaI GTT'AAC
Cuts at: 3024 3024
Size: 5434
KpnI G_GTAC'C
Cuts at: 2759 2759
Size: 5434
MfeI C'AATT_G
Cuts at: 3033 3033
Size: 5434
MluI A'CGCG_T
Cuts at: 5310 5310
Size: 5434
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2754 4548 4732 1305
Size: 1449 1794 184 2007
Fragments arranged by size:
2007 1794 1449 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2561 10
Size: 54 1425 343 729 2883
Fragments arranged by size:
2883 1425 729 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2030 3392 3751 3910 518
Size: 648 864 1362 359 159 2042
Fragments arranged by size:
2042 1362 864 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2850 3527 4675 4920 1606
Size: 1244 677 1148 245 2120
Fragments arranged by size:
2120 1244 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4882
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5434
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5434
NotI GC'GGCC_GC
Cuts at: 2881 2881
Size: 5434
NspI r_CATG'y
Cuts at: 1132 1229 2792 4337 1132
Size: 97 1563 1545 2229
Fragments arranged by size:
2229 1563 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2042 1348
Size: 694 4740
PciI A'CATG_T
Cuts at: 4333 4333
Size: 5434
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2498 1108
Size: 1390 4044
PmeI GTTT'AAAC
Cuts at: 2738 2738
Size: 5434
PmlI CAC'GTG
Cuts at: 2632 2632
Size: 5434
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3503 3535 5016 5048 3503
Size: 32 1481 32 3889
Fragments arranged by size:
3889 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2662 2662
Size: 5434
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5434
PsiI TTA'TAA
Cuts at: 3004 3004
Size: 5434
PstI C_TGCA'G
Cuts at: 830 2335 2786 830
Size: 1505 451 3478
Fragments arranged by size:
3478 1505 451
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5434
SacI G_AGCT'C
Cuts at: 2592 2592
Size: 5434
SalI G'TCGA_C
Cuts at: 1936 2776 1936
Size: 840 4594
SbfI CC_TGCA'GG
Cuts at: 2786 2786
Size: 5434
ScaI AGT'ACT
Cuts at: 3682 5394 3682
Size: 1712 3722
SfcI C'TryA_G
Cuts at: 826 1045 2331 2782 3917 4598 4789 826
Size: 219 1286 451 1135 681 191 1471
Fragments arranged by size:
1471 1286 1135 681 451 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5434
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5434
SmaI CCC'GGG
Cuts at: 1872 2761 1872
Size: 889 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3538 4439 4701 820
Size: 197 926 1595 901 262 1553
Fragments arranged by size:
1595 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3225 493
Size: 2732 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5434
SphI G_CATG'C
Cuts at: 1132 2792 1132
Size: 1660 3774
SspI AAT'ATT
Cuts at: 5 52 3358 5
Size: 47 3306 2081
Fragments arranged by size:
3306 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5434
StyI C'CwwG_G
Cuts at: 513 1065 2873 513
Size: 552 1808 3074
Fragments arranged by size:
3074 1808 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3461 3478 3631 3816 4261 325
Size: 1255 1881 17 153 185 445 1498
Fragments arranged by size:
1881 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3680 5392 96
Size: 274 80 33 51 1443 1703 1712 138
Fragments arranged by size:
1712 1703 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5394 5426 5394
Size: 32 5402
Tth111I GACn'n_nGTC
Cuts at: 1316 2605 1316
Size: 1289 4145
XbaI T'CTAG_A
Cuts at: 2770 2770
Size: 5434
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2499 2499
Size: 5434
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5434
XmaI C'CCGG_G
Cuts at: 1870 2759 1870
Size: 889 4545
XmnI GAAnn'nnTTC
Cuts at: 2139 3563 2139
Size: 1424 4010
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3242 276
Size: 53 83 186 936 1708 2468
Fragments arranged by size:
2468 1708 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI
PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI