Restriction map for
pFN21AE0661
(Circular) (Six-base) MAPSORT of: pFN21AE0661.seq Check: 2566 from: 1 to: 5413
pFN21AE0661 5413 bp
With 182 enzymes: SgfI *
October 20, 2009 10:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3223 278
Size: 53 83 186 936 1687 2468
Fragments arranged by size:
2468 1687 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2756 1937
Size: 819 4594
Acc65I G'GTAC_C
Cuts at: 2734 2734
Size: 5413
AclI AA'CG_TT
Cuts at: 1563 3540 3913 1563
Size: 1977 373 3063
Fragments arranged by size:
3063 1977 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3476 4860 5128 1632
Size: 1844 1384 268 1917
Fragments arranged by size:
1917 1844 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5216
AflIII A'CryG_T
Cuts at: 1203 2410 4312 5289 1203
Size: 1207 1902 977 1327
Fragments arranged by size:
1902 1327 1207 977
AgeI A'CCGG_T
Cuts at: 4244 4244
Size: 5413
AhdI GACnn_n'nnGTC
Cuts at: 4142 4142
Size: 5413
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5413
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5413
AlwNI CAG_nnn'CTG
Cuts at: 2429 2492 2495 2555 2651 2654 2692 3156 4728
Size: 63 3 60 96 3 38 464 1572
Cuts at: 4728 2429
Size: 3114
Fragments arranged by size:
3114 1572 464 96 63 60 38 3
3
ApaLI G'TGCA_C
Cuts at: 3470 4626 3470
Size: 1156 4257
ApoI r'AATT_y
Cuts at: 1493 2722 2948 3100 5209 1493
Size: 1229 226 152 2109 1697
Fragments arranged by size:
2109 1697 1229 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3967 160
Size: 872 2935 1606
Fragments arranged by size:
2935 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5413
AvaI C'yCGr_G
Cuts at: 1870 1943 2157 2738 1870
Size: 73 214 581 4545
Fragments arranged by size:
4545 581 214 73
BamHI G'GATC_C
Cuts at: 2215 2743 3110 2215
Size: 528 367 4518
Fragments arranged by size:
4518 528 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2617 2734 4189 618
Size: 325 217 627 830 117 1455 1842
Fragments arranged by size:
1842 1455 830 627 325 217 117
BanII G_rGCy'C
Cuts at: 1391 2732 1391
Size: 1341 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2095 928
Size: 971 196 4246
Fragments arranged by size:
4246 971 196
BbvCI CC'TCA_GC
Cuts at: 2685 2685
Size: 5413
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2077 2111 3604 3638 1903
Size: 34 50 34 56 34 1493 34 3678
Fragments arranged by size:
3678 1493 56 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2346 3305 4515 2346
Size: 959 1210 3244
Fragments arranged by size:
3244 1210 959
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5413
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2204 4024 136
Size: 107 122 71 631 605 532 1820 1525
Fragments arranged by size:
1820 1525 631 605 532 122 107 71
BglII A'GATC_T
Cuts at: 2040 5408 2040
Size: 3368 2045
BlpI GC'TnA_GC
Cuts at: 2830 2830
Size: 5413
Bme1580I G_kGCm'C
Cuts at: 1790 3474 4630 1790
Size: 1684 1156 2573
Fragments arranged by size:
2573 1684 1156
BmgBI CAC'GTC
Cuts at: 1318 2413 1318
Size: 1095 4318
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3144 4102 442
Size: 416 53 83 399 24 1727 958 1753
Fragments arranged by size:
1753 1727 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5413
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2484 2532 4073 1239
Size: 1245 48 1541 2579
Fragments arranged by size:
2579 1541 1245 48
Bpu10I CC'TnA_GC
Cuts at: 2685 2685
Size: 5413
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3538 4403 4701 3538
Size: 865 298 4250
Fragments arranged by size:
4250 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4076 882
Size: 617 2577 2219
Fragments arranged by size:
2577 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3204 493
Size: 711 2000 2702
Fragments arranged by size:
2702 2000 711
BsaBI GATnn'nnATC
Cuts at: 2220 5407 2220
Size: 3187 2226
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2076 3220 3602 4237
Size: 53 83 186 936 543 1144 382 635
Cuts at: 4237 275
Size: 1451
Fragments arranged by size:
1451 1144 936 635 543 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3846 4244 4518 4665 5076 3846
Size: 398 274 147 411 4183
Fragments arranged by size:
4183 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5188
Fragments arranged by size:
5188 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2132 1186
Size: 946 4467
BseYI C'CCAG_C
Cuts at: 2573 4616 5112 2573
Size: 2043 496 2874
Fragments arranged by size:
2874 2043 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2408 1163
Size: 573 672 4168
Fragments arranged by size:
4168 672 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2140 2863 3624 4652 1540
Size: 275 186 139 723 761 1028 2301
Fragments arranged by size:
2301 1028 761 723 275 186 139
BsiHKAI G_wGCw'C
Cuts at: 3474 3559 4630 3474
Size: 85 1071 4257
Fragments arranged by size:
4257 1071 85
BsmI GAATG_Cn'
Cuts at: 2712 2924 3017 2712
Size: 212 93 5108
Fragments arranged by size:
5108 212 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2732 3474 3559 4630 1391
Size: 399 942 742 85 1071 2174
Fragments arranged by size:
2174 1071 942 742 399 85
BspEI T'CCGG_A
Cuts at: 5076 5076
Size: 5413
BspHI T'CATG_A
Cuts at: 3302 5032 3302
Size: 1730 3683
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2365 2768 844
Size: 1521 403 3489
Fragments arranged by size:
3489 1521 403
BsrBI CCG'CTC
Cuts at: 1394 1823 3300 1394
Size: 429 1477 3507
Fragments arranged by size:
3507 1477 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3908 4082 65
Size: 1183 2660 174 1396
Fragments arranged by size:
2660 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2139 4057 4244 1700
Size: 110 329 1918 187 2869
Fragments arranged by size:
2869 1918 329 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5413
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5413
BssSI C'ACGA_G
Cuts at: 3473 4485 3473
Size: 1012 4401
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2495 2651 2654 794
Size: 1701 156 3 3553
Fragments arranged by size:
3553 1701 156 3
BstBI TT'CG_AA
Cuts at: 4217 4217
Size: 5413
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5413
BstYI r'GATC_y
Cuts at: 1972 2040 2215 2743 3110 3495 3512 4964 5050
Size: 68 175 528 367 385 17 1452 86
Cuts at: 5050 5062 5408 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 528 385 367 346 175 86
68 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2178 513
Size: 552 1113 3748
Fragments arranged by size:
3748 1113 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4674
Fragments arranged by size:
4674 683 56
BtsI GCAGTG_nn'
Cuts at: 2929 3723 3743 2929
Size: 794 20 4599
Fragments arranged by size:
4599 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5413
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5378
DraI TTT'AAA
Cuts at: 2717 2717
Size: 5413
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2152 4420 809
Size: 507 836 2268 1802
Fragments arranged by size:
2268 1802 836 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2671 2860 3749
Size: 54 1425 50 275 18 841 189 889
Cuts at: 3749 5378 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 841 275 189 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2860 1537
Size: 275 1048 4090
Fragments arranged by size:
4090 1048 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3343 1353
Size: 1990 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4000 4374 4520 387
Size: 1064 138 2411 374 146 1280
Fragments arranged by size:
2411 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2484 2532 3476 4073 4860 5128 1239
Size: 393 852 48 944 597 787 268 1524
Fragments arranged by size:
1524 944 852 787 597 393 268 48
EcoO109I rG'GnC_Cy
Cuts at: 2205 2205
Size: 5413
EcoRI G'AATT_C
Cuts at: 2722 3100 2722
Size: 378 5035
FspI TGC'GCA
Cuts at: 2087 3919 2087
Size: 1832 3581
HaeII r_GCGC'y
Cuts at: 1678 4560 1678
Size: 2882 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4054 4086 4128 4160 1373
Size: 32 135 32 2482 32 42 32 2626
Fragments arranged by size:
2626 2482 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2757 3003 669
Size: 988 281 819 246 3079
Fragments arranged by size:
3079 988 819 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5413
HpaI GTT'AAC
Cuts at: 3003 3003
Size: 5413
KpnI G_GTAC'C
Cuts at: 2738 2738
Size: 5413
MfeI C'AATT_G
Cuts at: 3012 3012
Size: 5413
MluI A'CGCG_T
Cuts at: 5289 5289
Size: 5413
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4527 4711 1305
Size: 3222 184 2007
Fragments arranged by size:
3222 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2673 10
Size: 54 1425 343 841 2750
Fragments arranged by size:
2750 1425 841 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3371 3730 3889 518
Size: 648 2205 359 159 2042
Fragments arranged by size:
2205 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2429 2492 2513 2552 2615 2630 2642 2651
Size: 823 63 21 39 63 15 12 9
Cuts at: 2651 2660 2692 2829 3506 4654 4899 1606
Size: 9 32 137 677 1148 245 2120
Fragments arranged by size:
2120 1148 823 677 245 137 63 63
39 32 21 15 12 9 9
NcoI C'CATG_G
Cuts at: 513 1065 2178 513
Size: 552 1113 3748
Fragments arranged by size:
3748 1113 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5413
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5413
NotI GC'GGCC_GC
Cuts at: 2860 2860
Size: 5413
NspI r_CATG'y
Cuts at: 1132 1229 2771 4316 1132
Size: 97 1542 1545 2229
Fragments arranged by size:
2229 1545 1542 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5413
PciI A'CATG_T
Cuts at: 4312 4312
Size: 5413
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5413
PmeI GTTT'AAAC
Cuts at: 2717 2717
Size: 5413
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3482 3514 4995 5027 3482
Size: 32 1481 32 3868
Fragments arranged by size:
3868 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5413
PsiI TTA'TAA
Cuts at: 2983 2983
Size: 5413
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2246 2278 2246
Size: 32 5381
PstI C_TGCA'G
Cuts at: 830 2560 2647 2665 2697 2765 830
Size: 1730 87 18 32 68 3478
Fragments arranged by size:
3478 1730 87 68 32 18
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5413
PvuII CAG'CTG
Cuts at: 2429 2492 2552 2630 2651 2692 2429
Size: 63 60 78 21 41 5150
Fragments arranged by size:
5150 78 63 60 41 21
SalI G'TCGA_C
Cuts at: 1936 2755 1936
Size: 819 4594
SbfI CC_TGCA'GG
Cuts at: 2765 2765
Size: 5413
ScaI AGT'ACT
Cuts at: 2093 3661 5373 2093
Size: 1568 1712 2133
Fragments arranged by size:
2133 1712 1568
SexAI A'CCwGG_T
Cuts at: 2293 2293
Size: 5413
SfcI C'TryA_G
Cuts at: 826 1045 2556 2643 2661 2693 2761 3896 4577
Size: 219 1511 87 18 32 68 1135 681
Cuts at: 4577 4768 826
Size: 191 1471
Fragments arranged by size:
1511 1471 1135 681 219 191 87 68
32 18
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5413
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5413
SmaI CCC'GGG
Cuts at: 1872 2159 2740 1872
Size: 287 581 4545
Fragments arranged by size:
4545 581 287
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3517 4418 4680 820
Size: 197 926 1574 901 262 1553
Fragments arranged by size:
1574 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3204 493
Size: 2711 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5413
SphI G_CATG'C
Cuts at: 1132 2771 1132
Size: 1639 3774
SspI AAT'ATT
Cuts at: 5 52 3337 5
Size: 47 3285 2081
Fragments arranged by size:
3285 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5413
StyI C'CwwG_G
Cuts at: 513 1065 2178 2279 2852 513
Size: 552 1113 101 573 3074
Fragments arranged by size:
3074 1113 573 552 101
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2718 3440 3457 3610 3795 4240 325
Size: 1255 1138 722 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1138 722 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2091 3659 5371
Size: 274 80 33 51 1443 114 1568 1712
Cuts at: 5371 96
Size: 138
Fragments arranged by size:
1712 1568 1443 274 138 114 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5373 5405 5373
Size: 32 5381
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5413
XbaI T'CTAG_A
Cuts at: 2749 2749
Size: 5413
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2254 2254
Size: 5413
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5413
XmaI C'CCGG_G
Cuts at: 1870 2157 2738 1870
Size: 287 581 4545
Fragments arranged by size:
4545 581 287
XmnI GAAnn'nnTTC
Cuts at: 3542 3542
Size: 5413
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3221 276
Size: 53 83 186 936 1687 2468
Fragments arranged by size:
2468 1687 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI RsrII SacI SacII SanDI SapI SfiI
SfoI SrfI SwaI