Restriction map for
pFN21AE0658
(Circular) (Six-base) MAPSORT of: pFN21AE0658.seq Check: 8872 from: 1 to: 5872
pFN21AE0658 5872 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2350 2506 2350
Size: 156 5716
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3682 278
Size: 53 83 186 936 2146 2468
Fragments arranged by size:
2468 2146 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2628 3215 1937
Size: 691 587 4594
Fragments arranged by size:
4594 691 587
Acc65I G'GTAC_C
Cuts at: 3193 3193
Size: 5872
AclI AA'CG_TT
Cuts at: 1563 3999 4372 1563
Size: 2436 373 3063
Fragments arranged by size:
3063 2436 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3080 3935 5319 5587 1632
Size: 1448 855 1384 268 1917
Fragments arranged by size:
1917 1448 1384 855 268
AfeI AGC'GCT
Cuts at: 3032 3032
Size: 5872
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5675
AflIII A'CryG_T
Cuts at: 1203 4771 5748 1203
Size: 3568 977 1327
Fragments arranged by size:
3568 1327 977
AgeI A'CCGG_T
Cuts at: 4703 4703
Size: 5872
AhdI GACnn_n'nnGTC
Cuts at: 4601 4601
Size: 5872
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5872
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5872
AlwNI CAG_nnn'CTG
Cuts at: 2284 2296 2437 2973 3615 5187 2284
Size: 12 141 536 642 1572 2969
Fragments arranged by size:
2969 1572 642 536 141 12
ApaI G_GGCC'C
Cuts at: 2328 2328
Size: 5872
ApaLI G'TGCA_C
Cuts at: 2519 3929 5085 2519
Size: 1410 1156 3306
Fragments arranged by size:
3306 1410 1156
ApoI r'AATT_y
Cuts at: 1493 3181 3407 3559 5668 1493
Size: 1688 226 152 2109 1697
Fragments arranged by size:
2109 1697 1688 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4426 160
Size: 872 3394 1606
Fragments arranged by size:
3394 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5872
AvaI C'yCGr_G
Cuts at: 1870 1943 3197 1870
Size: 73 1254 4545
Fragments arranged by size:
4545 1254 73
BamHI G'GATC_C
Cuts at: 3202 3569 3202
Size: 367 5505
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2731 3193 4648 618
Size: 325 217 627 944 462 1455 1842
Fragments arranged by size:
1842 1455 944 627 462 325 217
BanII G_rGCy'C
Cuts at: 1391 2328 3191 1391
Size: 937 863 4072
Fragments arranged by size:
4072 937 863
BbeI G_GCGC'C
Cuts at: 2735 2735
Size: 5872
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2548 2566 928
Size: 971 649 18 4234
Fragments arranged by size:
4234 971 649 18
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4063 4097 1903
Size: 34 2126 34 3678
Fragments arranged by size:
3678 2126 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3764 4974 3764
Size: 1210 4662
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5872
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4483 136
Size: 107 122 71 631 605 2811 1525
Fragments arranged by size:
2811 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2682 5867 2682
Size: 3185 2687
BlpI GC'TnA_GC
Cuts at: 2165 3289 2165
Size: 1124 4748
Bme1580I G_kGCm'C
Cuts at: 1790 2328 2523 3933 5089 1790
Size: 538 195 1410 1156 2573
Fragments arranged by size:
2573 1410 1156 538 195
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5872
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3603 4561 442
Size: 416 53 83 399 24 2186 958 1753
Fragments arranged by size:
2186 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2624 1056
Size: 1568 4304
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2050 3121 4532 1239
Size: 811 1071 1411 2579
Fragments arranged by size:
2579 1411 1071 811
Bpu10I CC'TnA_GC
Cuts at: 2246 3122 2246
Size: 876 4996
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2687 3997 4862 5160 2687
Size: 1310 865 298 3399
Fragments arranged by size:
3399 1310 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4535 882
Size: 617 3036 2219
Fragments arranged by size:
3036 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2484 3663 493
Size: 711 1280 1179 2702
Fragments arranged by size:
2702 1280 1179 711
BsaBI GATnn'nnATC
Cuts at: 5866 5866
Size: 5872
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2553 2732 2870 3070
Size: 53 83 186 936 1020 179 138 200
Cuts at: 3070 3679 4061 4696 275
Size: 609 382 635 1451
Fragments arranged by size:
1451 1020 936 635 609 382 200 186
179 138 83 53
BsaWI w'CCGG_w
Cuts at: 4305 4703 4977 5124 5535 4305
Size: 398 274 147 411 4642
Fragments arranged by size:
4642 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3095 3125 1178
Size: 30 165 30 1692 30 3925
Fragments arranged by size:
3925 1692 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3160 1186
Size: 1974 3898
BseYI C'CCAG_C
Cuts at: 2565 3156 5075 5571 2565
Size: 591 1919 496 2866
Fragments arranged by size:
2866 1919 591 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2906 3047 1163
Size: 573 1170 141 3988
Fragments arranged by size:
3988 1170 573 141
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2464 2727 3322 4083 5111 1540
Size: 275 186 463 263 595 761 1028 2301
Fragments arranged by size:
2301 1028 761 595 463 275 263 186
BsiHKAI G_wGCw'C
Cuts at: 2478 2523 2652 3933 4018 5089 2478
Size: 45 129 1281 85 1071 3261
Fragments arranged by size:
3261 1281 1071 129 85 45
BsmI GAATG_Cn'
Cuts at: 2179 3383 3476 2179
Size: 1204 93 4575
Fragments arranged by size:
4575 1204 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3074 3074
Size: 5872
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2328 2478 2523 2652 3191 3933 4018
Size: 399 538 150 45 129 539 742 85
Cuts at: 4018 5089 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 539 538 399 150 129
85 45
BspEI T'CCGG_A
Cuts at: 5535 5535
Size: 5872
BspHI T'CATG_A
Cuts at: 2235 3761 5491 2235
Size: 1526 1730 2616
Fragments arranged by size:
2616 1730 1526
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2340 2350 2397 2506 3227 844
Size: 1496 10 47 109 721 3489
Fragments arranged by size:
3489 1496 721 109 47 10
BsrBI CCG'CTC
Cuts at: 1394 1823 2169 3759 1394
Size: 429 346 1590 3507
Fragments arranged by size:
3507 1590 429 346
BsrDI GCAATG_nn'
Cuts at: 65 1248 4367 4541 65
Size: 1183 3119 174 1396
Fragments arranged by size:
3119 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2638 4516 4703 1700
Size: 110 828 1878 187 2869
Fragments arranged by size:
2869 1878 828 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5872
BssHII G'CGCG_C
Cuts at: 1925 2013 2120 2508 1925
Size: 88 107 388 5289
Fragments arranged by size:
5289 388 107 88
BssSI C'ACGA_G
Cuts at: 3023 3932 4944 3023
Size: 909 1012 3951
Fragments arranged by size:
3951 1012 909
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2695 794
Size: 1901 3971
BstBI TT'CG_AA
Cuts at: 4676 4676
Size: 5872
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5872
BstYI r'GATC_y
Cuts at: 1972 2682 2814 2985 3202 3569 3954 3971 5423
Size: 710 132 171 217 367 385 17 1452
Cuts at: 5423 5509 5521 5867 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 710 385 367 346 217 171
132 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2099 3165 513
Size: 552 939 95 1066 3220
Fragments arranged by size:
3220 1066 939 552 95
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2236 2769 3113 487
Size: 683 56 1010 533 344 3246
Fragments arranged by size:
3246 1010 683 533 344 56
BtsI GCAGTG_nn'
Cuts at: 2277 2478 3388 4182 4202 2277
Size: 201 910 794 20 3947
Fragments arranged by size:
3947 910 794 201 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5872
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5837
DraI TTT'AAA
Cuts at: 3176 3176
Size: 5872
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4879 809
Size: 507 3563 1802
Fragments arranged by size:
3563 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2421 2502 2724
Size: 54 1425 50 275 18 591 81 222
Cuts at: 2724 2955 3319 4208 5837 8
Size: 231 364 889 1629 43
Fragments arranged by size:
1629 1425 889 591 364 275 231 222
81 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2724 3319 1537
Size: 275 912 595 4090
Fragments arranged by size:
4090 912 595 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2231 3017 3802 1353
Size: 878 786 785 3423
Fragments arranged by size:
3423 878 786 785
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3037 4459 4833 4979 387
Size: 1064 138 1448 1422 374 146 1280
Fragments arranged by size:
1448 1422 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2050 3080 3121 3935 4532 5319 5587
Size: 393 418 1030 41 814 597 787 268
Cuts at: 5587 1239
Size: 1524
Fragments arranged by size:
1524 1030 814 787 597 418 393 268
41
EcoO109I rG'GnC_Cy
Cuts at: 2113 2591 2113
Size: 478 5394
EcoRI G'AATT_C
Cuts at: 3181 3559 3181
Size: 378 5494
FspI TGC'GCA
Cuts at: 2198 4378 2198
Size: 2180 3692
HaeII r_GCGC'y
Cuts at: 1678 2037 2058 2165 2195 2735 3034 5019 1678
Size: 359 21 107 30 540 299 1985 2531
Fragments arranged by size:
2531 1985 540 359 299 107 30 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4513 4545 4587 4619 1373
Size: 32 135 32 2941 32 42 32 2626
Fragments arranged by size:
2941 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3216 3462 669
Size: 988 281 1278 246 3079
Fragments arranged by size:
3079 1278 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5872
HpaI GTT'AAC
Cuts at: 3462 3462
Size: 5872
KasI G'GCGC_C
Cuts at: 2731 2731
Size: 5872
KpnI G_GTAC'C
Cuts at: 3197 3197
Size: 5872
MfeI C'AATT_G
Cuts at: 3471 3471
Size: 5872
MluI A'CGCG_T
Cuts at: 5748 5748
Size: 5872
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4986 5170 1305
Size: 3681 184 2007
Fragments arranged by size:
3681 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2423 2504 2957 10
Size: 54 1425 343 591 81 453 2925
Fragments arranged by size:
2925 1425 591 453 343 81 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2843 2945 3830 4189 4348 518
Size: 648 1677 102 885 359 159 2042
Fragments arranged by size:
2042 1677 885 648 359 159 102
MspA1I CmG'CkG
Cuts at: 1606 2101 2415 2437 2711 2717 2970 3288 3965
Size: 495 314 22 274 6 253 318 677
Cuts at: 3965 5113 5358 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 495 318 314 274 253
245 22 6
NarI GG'CG_CC
Cuts at: 2732 2732
Size: 5872
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4381
Fragments arranged by size:
4381 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5872
NheI G'CTAG_C
Cuts at: 1052 2620 1052
Size: 1568 4304
NotI GC'GGCC_GC
Cuts at: 3319 3319
Size: 5872
NspI r_CATG'y
Cuts at: 1132 1229 2374 2995 3230 4775 1132
Size: 97 1145 621 235 1545 2229
Fragments arranged by size:
2229 1545 1145 621 235 97
PasI CC'CwG_GG
Cuts at: 1348 2586 1348
Size: 1238 4634
PciI A'CATG_T
Cuts at: 4771 4771
Size: 5872
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5872
PmeI GTTT'AAAC
Cuts at: 3176 3176
Size: 5872
PmlI CAC'GTG
Cuts at: 2484 2484
Size: 5872
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3941 3973 5454 5486 3941
Size: 32 1481 32 4327
Fragments arranged by size:
4327 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2591 2591
Size: 5872
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5872
PsiI TTA'TAA
Cuts at: 3442 3442
Size: 5872
PspOMI G'GGCC_C
Cuts at: 2324 2324
Size: 5872
PstI C_TGCA'G
Cuts at: 830 2716 3224 830
Size: 1886 508 3478
Fragments arranged by size:
3478 1886 508
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5872
PvuII CAG'CTG
Cuts at: 2970 2970
Size: 5872
SacII CC_GC'GG
Cuts at: 2102 2102
Size: 5872
SalI G'TCGA_C
Cuts at: 1936 3214 1936
Size: 1278 4594
SanDI GG'GwC_CC
Cuts at: 2591 2591
Size: 5872
SbfI CC_TGCA'GG
Cuts at: 3224 3224
Size: 5872
ScaI AGT'ACT
Cuts at: 2354 4120 5832 2354
Size: 1766 1712 2394
Fragments arranged by size:
2394 1766 1712
SexAI A'CCwGG_T
Cuts at: 2259 2259
Size: 5872
SfcI C'TryA_G
Cuts at: 826 1045 2712 3220 4355 5036 5227 826
Size: 219 1667 508 1135 681 191 1471
Fragments arranged by size:
1667 1471 1135 681 508 219 191
SfoI GGC'GCC
Cuts at: 2733 2733
Size: 5872
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5872
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5872
SmaI CCC'GGG
Cuts at: 1872 3199 1872
Size: 1327 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2702 3976 4877 5139 820
Size: 197 926 759 1274 901 262 1553
Fragments arranged by size:
1553 1274 926 901 759 262 197
SnaBI TAC'GTA
Cuts at: 493 3663 493
Size: 3170 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5872
SphI G_CATG'C
Cuts at: 1132 3230 1132
Size: 2098 3774
SspI AAT'ATT
Cuts at: 5 52 3796 5
Size: 47 3744 2081
Fragments arranged by size:
3744 2081 47
StuI AGG'CCT
Cuts at: 1517 2600 1517
Size: 1083 4789
StyI C'CwwG_G
Cuts at: 513 1065 2004 2595 2805 2919 3311 513
Size: 552 939 591 210 114 392 3074
Fragments arranged by size:
3074 939 591 552 392 210 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3899 3916 4069 4254 4699 325
Size: 1255 2319 17 153 185 445 1498
Fragments arranged by size:
2319 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2352 4118 5830
Size: 274 80 33 51 1443 375 1766 1712
Cuts at: 5830 96
Size: 138
Fragments arranged by size:
1766 1712 1443 375 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2164 2196 5832 5864 2164
Size: 32 3636 32 2172
Fragments arranged by size:
3636 2172 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2552 1316
Size: 1236 4636
XbaI T'CTAG_A
Cuts at: 3208 3208
Size: 5872
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5872
XmaI C'CCGG_G
Cuts at: 1870 3197 1870
Size: 1327 4545
XmnI GAAnn'nnTTC
Cuts at: 4001 4001
Size: 5872
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3680 276
Size: 53 83 186 936 2146 2468
Fragments arranged by size:
2468 2146 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI
BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PspXI PsrI RsrII SacI SapI SfiI SrfI SwaI
XcmI