Restriction map for
pFN21AE0654
(Circular) (Six-base) MAPSORT of: pFN21AE0654.seq Check: 813 from: 1 to: 5863
pFN21AE0654 5863 bp
With 182 enzymes: SgfI *
October 20, 2009 10:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3673 278
Size: 53 83 186 936 2137 2468
Fragments arranged by size:
2468 2137 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2652 3206 1937
Size: 715 554 4594
Fragments arranged by size:
4594 715 554
Acc65I G'GTAC_C
Cuts at: 3184 3184
Size: 5863
AclI AA'CG_TT
Cuts at: 1563 3990 4363 1563
Size: 2427 373 3063
Fragments arranged by size:
3063 2427 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2382 3926 5310 5578 1632
Size: 750 1544 1384 268 1917
Fragments arranged by size:
1917 1544 1384 750 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5666
AflIII A'CryG_T
Cuts at: 1203 4762 5739 1203
Size: 3559 977 1327
Fragments arranged by size:
3559 1327 977
AgeI A'CCGG_T
Cuts at: 4694 4694
Size: 5863
AhdI GACnn_n'nnGTC
Cuts at: 4592 4592
Size: 5863
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5863
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2193 3009 3131 1184
Size: 1009 816 122 3916
Fragments arranged by size:
3916 1009 816 122
AlwNI CAG_nnn'CTG
Cuts at: 3606 5178 3606
Size: 1572 4291
ApaLI G'TGCA_C
Cuts at: 3920 5076 3920
Size: 1156 4707
ApoI r'AATT_y
Cuts at: 1493 2730 3172 3398 3550 5659 1493
Size: 1237 442 226 152 2109 1697
Fragments arranged by size:
2109 1697 1237 442 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4417 160
Size: 872 3385 1606
Fragments arranged by size:
3385 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5863
AvaI C'yCGr_G
Cuts at: 1870 1943 2409 3188 1870
Size: 73 466 779 4545
Fragments arranged by size:
4545 779 466 73
BamHI G'GATC_C
Cuts at: 3193 3560 3193
Size: 367 5496
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3184 4639 618
Size: 325 217 627 1397 1455 1842
Fragments arranged by size:
1842 1455 1397 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3182 1391
Size: 1791 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2533 3042 928
Size: 971 634 509 3749
Fragments arranged by size:
3749 971 634 509
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4054 4088 1903
Size: 34 2117 34 3678
Fragments arranged by size:
3678 2117 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2119 3755 4965 2119
Size: 1636 1210 3017
Fragments arranged by size:
3017 1636 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5863
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2931 3097 4474
Size: 107 122 71 631 605 1259 166 1377
Cuts at: 4474 136
Size: 1525
Fragments arranged by size:
1525 1377 1259 631 605 166 122 107
71
BglII A'GATC_T
Cuts at: 5858 5858
Size: 5863
BlpI GC'TnA_GC
Cuts at: 3280 3280
Size: 5863
Bme1580I G_kGCm'C
Cuts at: 1790 3924 5080 1790
Size: 2134 1156 2573
Fragments arranged by size:
2573 2134 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5863
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2554 3594 4552
Size: 416 53 83 399 24 1137 1040 958
Cuts at: 4552 442
Size: 1753
Fragments arranged by size:
1753 1137 1040 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5863
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4523 1239
Size: 3284 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3078 3988 4853 5151 3078
Size: 910 865 298 3790
Fragments arranged by size:
3790 910 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4526 882
Size: 617 3027 2219
Fragments arranged by size:
3027 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3654 493
Size: 711 2450 2702
Fragments arranged by size:
2702 2450 711
BsaBI GATnn'nnATC
Cuts at: 5857 5857
Size: 5863
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3670 4052 4687 275
Size: 53 83 186 936 2137 382 635 1451
Fragments arranged by size:
2137 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4296 4694 4968 5115 5526 4296
Size: 398 274 147 411 4633
Fragments arranged by size:
4633 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5638
Fragments arranged by size:
5638 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5863
BseYI C'CCAG_C
Cuts at: 5066 5562 5066
Size: 496 5367
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2808 3039 1163
Size: 573 1072 231 3987
Fragments arranged by size:
3987 1072 573 231
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3313 4074 5102 1540
Size: 275 186 1312 761 1028 2301
Fragments arranged by size:
2301 1312 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3924 4009 5080 3924
Size: 85 1071 4707
Fragments arranged by size:
4707 1071 85
BsmI GAATG_Cn'
Cuts at: 3374 3467 3374
Size: 93 5770
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3182 3924 4009 5080 1391
Size: 399 1392 742 85 1071 2174
Fragments arranged by size:
2174 1392 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5526 5526
Size: 5863
BspHI T'CATG_A
Cuts at: 2164 3752 5482 2164
Size: 1588 1730 2545
Fragments arranged by size:
2545 1730 1588
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3218 844
Size: 2374 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2567 3750 1394
Size: 429 744 1183 3507
Fragments arranged by size:
3507 1183 744 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4358 4532 65
Size: 1183 3110 174 1396
Fragments arranged by size:
3110 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4507 4694 1700
Size: 110 2697 187 2869
Fragments arranged by size:
2869 2697 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5863
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5863
BssSI C'ACGA_G
Cuts at: 3923 4935 3923
Size: 1012 4851
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2195 2795 794
Size: 1401 600 3862
Fragments arranged by size:
3862 1401 600
BstBI TT'CG_AA
Cuts at: 4667 4667
Size: 5863
BstEII G'GTnAC_C
Cuts at: 1184 2039 1184
Size: 855 5008
BstXI CCAn_nnnn'nTGG
Cuts at: 2519 2921 2942 2519
Size: 402 21 5440
Fragments arranged by size:
5440 402 21
BstYI r'GATC_y
Cuts at: 1972 3193 3560 3945 3962 5414 5500 5512 5858
Size: 1221 367 385 17 1452 86 12 346
Cuts at: 5858 1972
Size: 1977
Fragments arranged by size:
1977 1452 1221 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2653 2653
Size: 5863
BtgI C'CryG_G
Cuts at: 513 1065 2004 3059 513
Size: 552 939 1055 3317
Fragments arranged by size:
3317 1055 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5124
Fragments arranged by size:
5124 683 56
BtsI GCAGTG_nn'
Cuts at: 3379 4173 4193 3379
Size: 794 20 5049
Fragments arranged by size:
5049 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5863
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5828
DraI TTT'AAA
Cuts at: 3167 3167
Size: 5863
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4870 809
Size: 507 3554 1802
Fragments arranged by size:
3554 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2509 2923 2962
Size: 54 1425 50 275 18 679 414 39
Cuts at: 2962 3010 3310 4199 5828 8
Size: 48 300 889 1629 43
Fragments arranged by size:
1629 1425 889 679 414 300 275 54
50 48 43 39 18
EagI C'GGCC_G
Cuts at: 1537 1812 3310 1537
Size: 275 1498 4090
Fragments arranged by size:
4090 1498 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3793 1353
Size: 2440 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4450 4824 4970 387
Size: 1064 138 2861 374 146 1280
Fragments arranged by size:
2861 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2382 3926 4523 5310 5578 1239
Size: 393 750 1544 597 787 268 1524
Fragments arranged by size:
1544 1524 787 750 597 393 268
EcoRI G'AATT_C
Cuts at: 3172 3550 3172
Size: 378 5485
EcoRV GAT'ATC
Cuts at: 2909 2909
Size: 5863
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2882 2882
Size: 5863
FspI TGC'GCA
Cuts at: 4369 4369
Size: 5863
HaeII r_GCGC'y
Cuts at: 1678 5010 1678
Size: 3332 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2050 2082 4504 4536 4578
Size: 32 135 32 478 32 2422 32 42
Cuts at: 4578 4610 1373
Size: 32 2626
Fragments arranged by size:
2626 2422 478 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3207 3453 669
Size: 988 281 1269 246 3079
Fragments arranged by size:
3079 1269 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5863
HpaI GTT'AAC
Cuts at: 3453 3453
Size: 5863
KpnI G_GTAC'C
Cuts at: 3188 3188
Size: 5863
MfeI C'AATT_G
Cuts at: 2799 3462 2799
Size: 663 5200
MluI A'CGCG_T
Cuts at: 5739 5739
Size: 5863
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4977 5161 1305
Size: 3672 184 2007
Fragments arranged by size:
3672 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2511 2925 2964 3012 10
Size: 54 1425 343 679 414 39 48 2861
Fragments arranged by size:
2861 1425 679 414 343 54 48 39
MslI CAynn'nnrTG
Cuts at: 518 1166 2175 2322 2784 3821 4180 4339 518
Size: 648 1009 147 462 1037 359 159 2042
Fragments arranged by size:
2042 1037 1009 648 462 359 159 147
MspA1I CmG'CkG
Cuts at: 1606 2132 2183 3279 3956 5104 5349 1606
Size: 526 51 1096 677 1148 245 2120
Fragments arranged by size:
2120 1148 1096 677 526 245 51
NcoI C'CATG_G
Cuts at: 513 1065 2004 3059 513
Size: 552 939 1055 3317
Fragments arranged by size:
3317 1055 939 552
NdeI CA'TA_TG
Cuts at: 387 2581 2608 2998 387
Size: 2194 27 390 3252
Fragments arranged by size:
3252 2194 390 27
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5863
NotI GC'GGCC_GC
Cuts at: 3310 3310
Size: 5863
NspI r_CATG'y
Cuts at: 1132 1229 2798 3115 3221 4766 1132
Size: 97 1569 317 106 1545 2229
Fragments arranged by size:
2229 1569 1545 317 106 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5863
PciI A'CATG_T
Cuts at: 4762 4762
Size: 5863
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3077 1108
Size: 1969 3894
PmeI GTTT'AAAC
Cuts at: 3167 3167
Size: 5863
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3932 3964 5445 5477 3932
Size: 32 1481 32 4318
Fragments arranged by size:
4318 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5863
PsiI TTA'TAA
Cuts at: 3433 3433
Size: 5863
PstI C_TGCA'G
Cuts at: 830 2188 3215 830
Size: 1358 1027 3478
Fragments arranged by size:
3478 1358 1027
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5863
PvuII CAG'CTG
Cuts at: 2132 2183 2132
Size: 51 5812
SalI G'TCGA_C
Cuts at: 1936 3205 1936
Size: 1269 4594
SbfI CC_TGCA'GG
Cuts at: 3215 3215
Size: 5863
ScaI AGT'ACT
Cuts at: 4111 5823 4111
Size: 1712 4151
SfcI C'TryA_G
Cuts at: 826 1045 2184 3211 4346 5027 5218 826
Size: 219 1139 1027 1135 681 191 1471
Fragments arranged by size:
1471 1139 1135 1027 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2931 2931
Size: 5863
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5863
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5863
SmaI CCC'GGG
Cuts at: 1872 3190 1872
Size: 1318 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3093 3967 4868 5130 820
Size: 197 926 1150 874 901 262 1553
Fragments arranged by size:
1553 1150 926 901 874 262 197
SnaBI TAC'GTA
Cuts at: 493 3654 493
Size: 3161 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5863
SphI G_CATG'C
Cuts at: 1132 3221 1132
Size: 2089 3774
SspI AAT'ATT
Cuts at: 5 52 3787 5
Size: 47 3735 2081
Fragments arranged by size:
3735 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5863
StyI C'CwwG_G
Cuts at: 513 1065 2004 3059 3302 513
Size: 552 939 1055 243 3074
Fragments arranged by size:
3074 1055 939 552 243
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2495 3890 3907 4060 4245 4690 325
Size: 1255 915 1395 17 153 185 445 1498
Fragments arranged by size:
1498 1395 1255 915 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2576 2645 4109
Size: 274 80 33 51 1443 599 69 1464
Cuts at: 4109 5821 96
Size: 1712 138
Fragments arranged by size:
1712 1464 1443 599 274 138 80 69
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5823 5855 5823
Size: 32 5831
Tth111I GACn'n_nGTC
Cuts at: 1316 3046 1316
Size: 1730 4133
XbaI T'CTAG_A
Cuts at: 3199 3199
Size: 5863
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2330 2500 2519 2330
Size: 170 19 5674
Fragments arranged by size:
5674 170 19
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5863
XmaI C'CCGG_G
Cuts at: 1870 3188 1870
Size: 1318 4545
XmnI GAAnn'nnTTC
Cuts at: 3992 3992
Size: 5863
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3671 276
Size: 53 83 186 936 2137 2468
Fragments arranged by size:
2468 2137 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I DraIII
EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfoI SrfI SwaI