Restriction map for
pFN21AE0644
(Circular) (Six-base) MAPSORT of: pFN21AE0644.seq Check: 5645 from: 1 to: 5998
pFN21AE0644 5998 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3808 278
Size: 53 83 186 936 2272 2468
Fragments arranged by size:
2468 2272 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2109 2381 3341 1937
Size: 172 272 960 4594
Fragments arranged by size:
4594 960 272 172
Acc65I G'GTAC_C
Cuts at: 3319 3319
Size: 5998
AclI AA'CG_TT
Cuts at: 1563 4125 4498 1563
Size: 2562 373 3063
Fragments arranged by size:
3063 2562 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4061 5445 5713 1632
Size: 2429 1384 268 1917
Fragments arranged by size:
2429 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5801
AflIII A'CryG_T
Cuts at: 1203 4897 5874 1203
Size: 3694 977 1327
Fragments arranged by size:
3694 1327 977
AgeI A'CCGG_T
Cuts at: 4829 4829
Size: 5998
AhdI GACnn_n'nnGTC
Cuts at: 2105 4727 2105
Size: 2622 3376
AleI CACnn'nnGTG
Cuts at: 1166 2159 2542 1166
Size: 993 383 4622
Fragments arranged by size:
4622 993 383
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2246 2381 1184
Size: 1062 135 4801
Fragments arranged by size:
4801 1062 135
AlwNI CAG_nnn'CTG
Cuts at: 3741 5313 3741
Size: 1572 4426
ApaLI G'TGCA_C
Cuts at: 2630 4055 5211 2630
Size: 1425 1156 3417
Fragments arranged by size:
3417 1425 1156
ApoI r'AATT_y
Cuts at: 1493 3094 3274 3307 3533 3685 5794 1493
Size: 1601 180 33 226 152 2109 1697
Fragments arranged by size:
2109 1697 1601 226 180 152 33
AseI AT'TA_AT
Cuts at: 160 1032 4552 160
Size: 872 3520 1606
Fragments arranged by size:
3520 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5998
AvaI C'yCGr_G
Cuts at: 1870 1943 3323 1870
Size: 73 1380 4545
Fragments arranged by size:
4545 1380 73
BamHI G'GATC_C
Cuts at: 3328 3695 3328
Size: 367 5631
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2365 2433 2863 3319 4774
Size: 325 217 627 578 68 430 456 1455
Cuts at: 4774 618
Size: 1842
Fragments arranged by size:
1842 1455 627 578 456 430 325 217
68
BanII G_rGCy'C
Cuts at: 1391 3317 1391
Size: 1926 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5027
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2055 4189 4223 1903
Size: 34 50 34 34 2134 34 3678
Fragments arranged by size:
3678 2134 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3890 5100 3890
Size: 1210 4788
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5998
BfrBI ATG'CAT
Cuts at: 3002 3002
Size: 5998
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2141 4609
Size: 107 122 71 631 605 337 132 2468
Cuts at: 4609 136
Size: 1525
Fragments arranged by size:
2468 1525 631 605 337 132 122 107
71
BglII A'GATC_T
Cuts at: 2640 5993 2640
Size: 3353 2645
BlpI GC'TnA_GC
Cuts at: 3415 3415
Size: 5998
Bme1580I G_kGCm'C
Cuts at: 1790 2634 2866 4059 5215 1790
Size: 844 232 1193 1156 2573
Fragments arranged by size:
2573 1193 1156 844 232
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5998
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3729 4687 442
Size: 416 53 83 399 24 2312 958 1753
Fragments arranged by size:
2312 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5998
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2053 2053
Size: 5998
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2300 3060 4658 1239
Size: 1061 760 1598 2579
Fragments arranged by size:
2579 1598 1061 760
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2582 4123 4988 5286 2582
Size: 1541 865 298 3294
Fragments arranged by size:
3294 1541 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4661 882
Size: 617 3162 2219
Fragments arranged by size:
3162 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2209 2551 3789 493
Size: 711 1005 342 1238 2702
Fragments arranged by size:
2702 1238 1005 711 342
BsaBI GATnn'nnATC
Cuts at: 5992 5992
Size: 5998
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3805 4187 4822 275
Size: 53 83 186 936 2272 382 635 1451
Fragments arranged by size:
2272 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4431 4829 5103 5250 5661 4431
Size: 398 274 147 411 4768
Fragments arranged by size:
4768 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3058 3088 1178
Size: 30 165 30 1655 30 4088
Fragments arranged by size:
4088 1655 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3233 1186
Size: 2047 3951
BseYI C'CCAG_C
Cuts at: 2338 2859 5201 5697 2338
Size: 521 2342 496 2639
Fragments arranged by size:
2639 2342 521 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5425
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2102 3448 4209 5237 1540
Size: 275 186 101 1346 761 1028 2301
Fragments arranged by size:
2301 1346 1028 761 275 186 101
BsiHKAI G_wGCw'C
Cuts at: 2154 2166 2634 4059 4144 5215 2154
Size: 12 468 1425 85 1071 2937
Fragments arranged by size:
2937 1425 1071 468 85 12
BsmI GAATG_Cn'
Cuts at: 2298 3173 3509 3602 2298
Size: 875 336 93 4694
Fragments arranged by size:
4694 875 336 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2154 2166 2634 2866 3317 4059 4144
Size: 399 364 12 468 232 451 742 85
Cuts at: 4144 5215 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 468 451 399 364 232
85 12
BspEI T'CCGG_A
Cuts at: 5661 5661
Size: 5998
BspHI T'CATG_A
Cuts at: 3887 5617 3887
Size: 1730 4268
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2286 2423 3353 844
Size: 1442 137 930 3489
Fragments arranged by size:
3489 1442 930 137
BsrBI CCG'CTC
Cuts at: 1394 1823 2104 3885 1394
Size: 429 281 1781 3507
Fragments arranged by size:
3507 1781 429 281
BsrDI GCAATG_nn'
Cuts at: 65 1248 2197 2418 4493 4667 65
Size: 1183 949 221 2075 174 1396
Fragments arranged by size:
2075 1396 1183 949 221 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2091 2156 4642 4829 1700
Size: 110 281 65 2486 187 2869
Fragments arranged by size:
2869 2486 281 187 110 65
BsrGI T'GTAC_A
Cuts at: 96 2703 96
Size: 2607 3391
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5998
BssSI C'ACGA_G
Cuts at: 2147 4058 5070 2147
Size: 1911 1012 3075
Fragments arranged by size:
3075 1911 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5998
BstBI TT'CG_AA
Cuts at: 2764 4802 2764
Size: 2038 3960
BstEII G'GTnAC_C
Cuts at: 1184 2212 1184
Size: 1028 4970
BstYI r'GATC_y
Cuts at: 1972 2640 3328 3695 4080 4097 5549 5635 5647
Size: 668 688 367 385 17 1452 86 12
Cuts at: 5647 5993 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 688 668 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5446
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2065 487
Size: 683 56 839 4420
Fragments arranged by size:
4420 839 683 56
BtsI GCAGTG_nn'
Cuts at: 3514 4308 4328 3514
Size: 794 20 5184
Fragments arranged by size:
5184 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5998
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5963
DraI TTT'AAA
Cuts at: 2458 3302 2458
Size: 844 5154
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5005 809
Size: 507 3689 1802
Fragments arranged by size:
3689 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3445 4334 5963
Size: 54 1425 50 275 18 1615 889 1629
Cuts at: 5963 8
Size: 43
Fragments arranged by size:
1629 1615 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3445 1537
Size: 275 1633 4090
Fragments arranged by size:
4090 1633 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2607 2675 3928 1353
Size: 1254 68 1253 3423
Fragments arranged by size:
3423 1254 1253 68
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4585 4959 5105 387
Size: 1064 138 2996 374 146 1280
Fragments arranged by size:
2996 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2300 3060 4061 4658 5445 5713 1239
Size: 393 668 760 1001 597 787 268 1524
Fragments arranged by size:
1524 1001 787 760 668 597 393 268
EcoRI G'AATT_C
Cuts at: 3274 3307 3685 3274
Size: 33 378 5587
Fragments arranged by size:
5587 378 33
FspI TGC'GCA
Cuts at: 2234 4504 2234
Size: 2270 3728
FspAI rTGC'GCAy
Cuts at: 2234 2234
Size: 5998
HaeII r_GCGC'y
Cuts at: 1678 2015 2183 2270 5145 1678
Size: 337 168 87 2875 2531
Fragments arranged by size:
2875 2531 337 168 87
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2091 2123 4639 4671 4713
Size: 32 135 32 519 32 2516 32 42
Cuts at: 4713 4745 1373
Size: 32 2626
Fragments arranged by size:
2626 2516 519 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3342 3588 669
Size: 988 281 1404 246 3079
Fragments arranged by size:
3079 1404 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5998
HpaI GTT'AAC
Cuts at: 3588 3588
Size: 5998
KpnI G_GTAC'C
Cuts at: 3323 3323
Size: 5998
MfeI C'AATT_G
Cuts at: 3597 3597
Size: 5998
MluI A'CGCG_T
Cuts at: 5874 5874
Size: 5998
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2352 2997 5112 5296 1305
Size: 1047 645 2115 184 2007
Fragments arranged by size:
2115 2007 1047 645 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4176
Fragments arranged by size:
4176 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2159 2423 2542 2825 3956 4315 4474
Size: 648 993 264 119 283 1131 359 159
Cuts at: 4474 518
Size: 2042
Fragments arranged by size:
2042 1131 993 648 359 283 264 159
119
MspA1I CmG'CkG
Cuts at: 1606 2387 3414 4091 5239 5484 1606
Size: 781 1027 677 1148 245 2120
Fragments arranged by size:
2120 1148 1027 781 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5446
NdeI CA'TA_TG
Cuts at: 387 2883 387
Size: 2496 3502
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5998
NotI GC'GGCC_GC
Cuts at: 3445 3445
Size: 5998
NsiI A_TGCA'T
Cuts at: 3004 3004
Size: 5998
NspI r_CATG'y
Cuts at: 1132 1229 3299 3356 4901 1132
Size: 97 2070 57 1545 2229
Fragments arranged by size:
2229 2070 1545 97 57
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5998
PciI A'CATG_T
Cuts at: 4897 4897
Size: 5998
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5998
PmeI GTTT'AAAC
Cuts at: 3302 3302
Size: 5998
PmlI CAC'GTG
Cuts at: 2551 2551
Size: 5998
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4067 4099 5580 5612 4067
Size: 32 1481 32 4453
Fragments arranged by size:
4453 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5998
PsiI TTA'TAA
Cuts at: 3568 3568
Size: 5998
PstI C_TGCA'G
Cuts at: 830 3043 3350 830
Size: 2213 307 3478
Fragments arranged by size:
3478 2213 307
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5998
SalI G'TCGA_C
Cuts at: 1936 3340 1936
Size: 1404 4594
SbfI CC_TGCA'GG
Cuts at: 3350 3350
Size: 5998
ScaI AGT'ACT
Cuts at: 4246 5958 4246
Size: 1712 4286
SfcI C'TryA_G
Cuts at: 826 1045 3039 3346 4481 5162 5353 826
Size: 219 1994 307 1135 681 191 1471
Fragments arranged by size:
1994 1471 1135 681 307 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5998
SgrAI Cr'CCGG_yG
Cuts at: 1700 2156 1700
Size: 456 5542
SmaI CCC'GGG
Cuts at: 1872 3325 1872
Size: 1453 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2561 4102 5003 5265 820
Size: 197 926 618 1541 901 262 1553
Fragments arranged by size:
1553 1541 926 901 618 262 197
SnaBI TAC'GTA
Cuts at: 493 3789 493
Size: 3296 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5998
SphI G_CATG'C
Cuts at: 1132 3356 1132
Size: 2224 3774
SspI AAT'ATT
Cuts at: 5 52 3922 5
Size: 47 3870 2081
Fragments arranged by size:
3870 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5998
StyI C'CwwG_G
Cuts at: 513 1065 2262 3437 513
Size: 552 1197 1175 3074
Fragments arranged by size:
3074 1197 1175 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2554 4025 4042 4195 4380 4825 325
Size: 1255 974 1471 17 153 185 445 1498
Fragments arranged by size:
1498 1471 1255 974 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2703 4244 5956
Size: 274 80 33 51 1443 726 1541 1712
Cuts at: 5956 96
Size: 138
Fragments arranged by size:
1712 1541 1443 726 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5958 5990 5958
Size: 32 5966
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5998
XbaI T'CTAG_A
Cuts at: 3334 3334
Size: 5998
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5998
XmaI C'CCGG_G
Cuts at: 1870 3323 1870
Size: 1453 4545
XmnI GAAnn'nnTTC
Cuts at: 4127 4127
Size: 5998
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3806 276
Size: 53 83 186 936 2272 2468
Fragments arranged by size:
2468 2272 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI KasI NaeI
NarI NgoMIV NruI PacI PfoI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI