Restriction map for
pFN21AE0643
(Circular) (Six-base) MAPSORT of: pFN21AE0643.seq Check: 8101 from: 1 to: 6007
pFN21AE0643 6007 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2697 2697
Size: 6007
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3817 278
Size: 53 83 186 936 2281 2468
Fragments arranged by size:
2468 2281 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2697 3057 3350 1937
Size: 760 360 293 4594
Fragments arranged by size:
4594 760 360 293
Acc65I G'GTAC_C
Cuts at: 3328 3328
Size: 6007
AclI AA'CG_TT
Cuts at: 1563 4134 4507 1563
Size: 2571 373 3063
Fragments arranged by size:
3063 2571 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2906 4070 5454 5722 1632
Size: 1274 1164 1384 268 1917
Fragments arranged by size:
1917 1384 1274 1164 268
AfeI AGC'GCT
Cuts at: 3040 3040
Size: 6007
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5810
AflIII A'CryG_T
Cuts at: 1203 2289 2700 4906 5883 1203
Size: 1086 411 2206 977 1327
Fragments arranged by size:
2206 1327 1086 977 411
AgeI A'CCGG_T
Cuts at: 4838 4838
Size: 6007
AhdI GACnn_n'nnGTC
Cuts at: 4736 4736
Size: 6007
AleI CACnn'nnGTG
Cuts at: 1166 2189 1166
Size: 1023 4984
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6007
AlwNI CAG_nnn'CTG
Cuts at: 2386 2884 3750 5322 2386
Size: 498 866 1572 3071
Fragments arranged by size:
3071 1572 866 498
ApaI G_GGCC'C
Cuts at: 2223 2234 2976 3165 2223
Size: 11 742 189 5065
Fragments arranged by size:
5065 742 189 11
ApaLI G'TGCA_C
Cuts at: 4064 5220 4064
Size: 1156 4851
ApoI r'AATT_y
Cuts at: 1493 3316 3542 3694 5803 1493
Size: 1823 226 152 2109 1697
Fragments arranged by size:
2109 1823 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2613 2613
Size: 6007
AseI AT'TA_AT
Cuts at: 160 1032 4561 160
Size: 872 3529 1606
Fragments arranged by size:
3529 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6007
AvaI C'yCGr_G
Cuts at: 1870 1943 2438 3165 3332 1870
Size: 73 495 727 167 4545
Fragments arranged by size:
4545 727 495 167 73
BamHI G'GATC_C
Cuts at: 3337 3704 3337
Size: 367 5640
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2041 2405 2685 2787 3328
Size: 325 217 627 254 364 280 102 541
Cuts at: 3328 4783 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 541 364 325 280 254
217 102
BanII G_rGCy'C
Cuts at: 1391 2107 2223 2234 2343 2834 2845 2976 3119
Size: 716 116 11 109 491 11 131 143
Cuts at: 3119 3165 3326 1391
Size: 46 161 4072
Fragments arranged by size:
4072 716 491 161 143 131 116 109
46 11 11
BbeI G_GCGC'C
Cuts at: 2045 2409 2045
Size: 364 5643
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2453 928
Size: 971 554 4482
Fragments arranged by size:
4482 971 554
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4198 4232 1903
Size: 34 2261 34 3678
Fragments arranged by size:
3678 2261 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3899 5109 3899
Size: 1210 4797
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6007
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2208 2683 2807
Size: 107 122 71 631 605 536 475 124
Cuts at: 2807 2951 4618 136
Size: 144 1667 1525
Fragments arranged by size:
1667 1525 631 605 536 475 144 124
122 107 71
BglII A'GATC_T
Cuts at: 6002 6002
Size: 6007
BlpI GC'TnA_GC
Cuts at: 3424 3424
Size: 6007
Bme1580I G_kGCm'C
Cuts at: 1790 2061 2223 2234 2303 2494 2976 3165 4068
Size: 271 162 11 69 191 482 189 903
Cuts at: 4068 5224 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 903 482 271 191 189 162
69 11
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6007
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2995 3738 4696
Size: 416 53 83 399 24 1578 743 958
Cuts at: 4696 442
Size: 1753
Fragments arranged by size:
1753 1578 958 743 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6007
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4667 1239
Size: 3428 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4132 4997 5295 4132
Size: 865 298 4844
Fragments arranged by size:
4844 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2084 4670 882
Size: 617 585 2586 2219
Fragments arranged by size:
2586 2219 617 585
BsaAI yAC'GTr
Cuts at: 493 1204 3798 493
Size: 711 2594 2702
Fragments arranged by size:
2702 2594 711
BsaBI GATnn'nnATC
Cuts at: 6001 6001
Size: 6007
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2042 2406 2751 3814
Size: 53 83 186 936 509 364 345 1063
Cuts at: 3814 4196 4831 275
Size: 382 635 1451
Fragments arranged by size:
1451 1063 936 635 509 382 364 345
186 83 53
BsaWI w'CCGG_w
Cuts at: 4440 4838 5112 5259 5670 4440
Size: 398 274 147 411 4777
Fragments arranged by size:
4777 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2756 2786 1178
Size: 30 165 30 1353 30 4399
Fragments arranged by size:
4399 1353 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3170 1186
Size: 1984 4023
BseYI C'CCAG_C
Cuts at: 2068 2073 2123 2332 2852 3112 5210 5706 2068
Size: 5 50 209 520 260 2098 496 2369
Fragments arranged by size:
2369 2098 520 496 260 209 50 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2285 2897 1163
Size: 573 549 612 4273
Fragments arranged by size:
4273 612 573 549
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2473 2477 2625 2795 2958 3457
Size: 275 186 472 4 148 170 163 499
Cuts at: 3457 4218 5246 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1028 761 499 472 275 186 170
163 148 4
BsiHKAI G_wGCw'C
Cuts at: 2430 4068 4153 5224 2430
Size: 1638 85 1071 3213
Fragments arranged by size:
3213 1638 1071 85
BsmI GAATG_Cn'
Cuts at: 3518 3611 3518
Size: 93 5914
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2061 2107 2223 2234 2303 2343 2430
Size: 399 271 46 116 11 69 40 87
Cuts at: 2430 2494 2834 2845 2976 3119 3165 3326 4068
Size: 64 340 11 131 143 46 161 742
Cuts at: 4068 4153 5224 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 340 271 161 143
131 116 87 85 69 64 46 46
40 11 11
BspEI T'CCGG_A
Cuts at: 5670 5670
Size: 6007
BspHI T'CATG_A
Cuts at: 3896 5626 3896
Size: 1730 4277
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2683 2697 3362 844
Size: 1839 14 665 3489
Fragments arranged by size:
3489 1839 665 14
BsrBI CCG'CTC
Cuts at: 1394 1823 3894 1394
Size: 429 2071 3507
Fragments arranged by size:
3507 2071 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4502 4676 65
Size: 1183 3254 174 1396
Fragments arranged by size:
3254 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2038 2445 2472 2790 3084 4651 4838
Size: 110 228 407 27 318 294 1567 187
Cuts at: 4838 1700
Size: 2869
Fragments arranged by size:
2869 1567 407 318 294 228 187 110
27
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6007
BssHII G'CGCG_C
Cuts at: 1925 2449 2613 2856 3129 1925
Size: 524 164 243 273 4803
Fragments arranged by size:
4803 524 273 243 164
BssSI C'ACGA_G
Cuts at: 2420 4067 5079 2420
Size: 1647 1012 3348
Fragments arranged by size:
3348 1647 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2386 794
Size: 1592 4415
BstBI TT'CG_AA
Cuts at: 4811 4811
Size: 6007
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6007
BstXI CCAn_nnnn'nTGG
Cuts at: 2068 2068
Size: 6007
BstYI r'GATC_y
Cuts at: 1972 2011 2661 3337 3704 4089 4106 5558 5644
Size: 39 650 676 367 385 17 1452 86
Cuts at: 5644 5656 6002 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 676 650 385 367 346 86
39 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2352 2656 2805 2976 513
Size: 552 939 348 304 149 171 3544
Fragments arranged by size:
3544 939 552 348 304 171 149
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2416 3041 487
Size: 683 56 1190 625 3453
Fragments arranged by size:
3453 1190 683 625 56
BtsI GCAGTG_nn'
Cuts at: 2519 3523 4317 4337 2519
Size: 1004 794 20 4189
Fragments arranged by size:
4189 1004 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6007
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5972
DraI TTT'AAA
Cuts at: 3311 3311
Size: 6007
DraIII CAC_nnn'GTG
Cuts at: 2422 2422
Size: 6007
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5014 809
Size: 507 3698 1802
Fragments arranged by size:
3698 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2349 2470 2474
Size: 54 1425 50 275 18 519 121 4
Cuts at: 2474 2622 2636 2761 2792 2955 3030 3454 4343
Size: 148 14 125 31 163 75 424 889
Cuts at: 4343 5972 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 519 424 275 163 148
125 121 75 54 50 43 31 18
14 4
EagI C'GGCC_G
Cuts at: 1537 1812 2470 2474 2622 2792 2955 3454 1537
Size: 275 658 4 148 170 163 499 4090
Fragments arranged by size:
4090 658 499 275 170 163 148 4
EarI CTCTTCn'nnn_
Cuts at: 1353 3068 3937 1353
Size: 1715 869 3423
Fragments arranged by size:
3423 1715 869
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2126 2294 4594 4968 5114 387
Size: 1064 138 537 168 2300 374 146 1280
Fragments arranged by size:
2300 1280 1064 537 374 168 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2906 4070 4667 5454 5722 1239
Size: 393 1274 1164 597 787 268 1524
Fragments arranged by size:
1524 1274 1164 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2220 2231 2359 2800 2998 3161 3162 2220
Size: 11 128 441 198 163 1 5065
Fragments arranged by size:
5065 441 198 163 128 11 1
EcoRI G'AATT_C
Cuts at: 3316 3694 3316
Size: 378 5629
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2269 2269
Size: 6007
FseI GG_CCGG'CC
Cuts at: 2476 2476
Size: 6007
FspI TGC'GCA
Cuts at: 4513 4513
Size: 6007
HaeII r_GCGC'y
Cuts at: 1678 2045 2309 2409 2446 2534 2819 2909 3014
Size: 367 264 100 37 88 285 90 105
Cuts at: 3014 3042 3182 5154 1678
Size: 28 140 1972 2531
Fragments arranged by size:
2531 1972 367 285 264 140 105 100
90 88 37 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2552 2584 2821 2853 2987
Size: 32 135 32 980 32 237 32 134
Cuts at: 2987 3019 4648 4680 4722 4754 1373
Size: 32 1629 32 42 32 2626
Fragments arranged by size:
2626 1629 980 237 135 134 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3351 3597 669
Size: 988 281 1413 246 3079
Fragments arranged by size:
3079 1413 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6007
HpaI GTT'AAC
Cuts at: 3597 3597
Size: 6007
KasI G'GCGC_C
Cuts at: 2041 2405 2041
Size: 364 5643
KpnI G_GTAC'C
Cuts at: 3332 3332
Size: 6007
MfeI C'AATT_G
Cuts at: 3606 3606
Size: 6007
MluI A'CGCG_T
Cuts at: 2289 2700 5883 2289
Size: 411 3183 2413
Fragments arranged by size:
3183 2413 411
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5121 5305 1305
Size: 3816 184 2007
Fragments arranged by size:
3816 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3032 10
Size: 54 1425 343 1200 2985
Fragments arranged by size:
2985 1425 1200 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2054 2162 2189 3965 4324 4483 518
Size: 648 888 108 27 1776 359 159 2042
Fragments arranged by size:
2042 1776 888 648 359 159 108 27
MspA1I CmG'CkG
Cuts at: 1606 2269 2317 2383 2479 2575 2807 2978 3100
Size: 663 48 66 96 96 232 171 122
Cuts at: 3100 3235 3423 4100 5248 5493 1606
Size: 135 188 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 663 245 232 188 171
135 122 96 96 66 48
NaeI GCC'GGC
Cuts at: 2040 2447 2474 3086 2040
Size: 407 27 612 4961
Fragments arranged by size:
4961 612 407 27
NarI GG'CG_CC
Cuts at: 2042 2406 2042
Size: 364 5643
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4516
Fragments arranged by size:
4516 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6007
NgoMIV G'CCGG_C
Cuts at: 2038 2445 2472 3084 2038
Size: 407 27 612 4961
Fragments arranged by size:
4961 612 407 27
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6007
NotI GC'GGCC_GC
Cuts at: 2955 3454 2955
Size: 499 5508
NspI r_CATG'y
Cuts at: 1132 1229 3365 4910 1132
Size: 97 2136 1545 2229
Fragments arranged by size:
2229 2136 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2223 2503 1348
Size: 875 280 4852
Fragments arranged by size:
4852 875 280
PciI A'CATG_T
Cuts at: 4906 4906
Size: 6007
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2422 1108
Size: 1314 4693
PmeI GTTT'AAAC
Cuts at: 3311 3311
Size: 6007
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4076 4108 5589 5621 4076
Size: 32 1481 32 4462
Fragments arranged by size:
4462 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2359 2359
Size: 6007
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6007
PsiI TTA'TAA
Cuts at: 3577 3577
Size: 6007
PspOMI G'GGCC_C
Cuts at: 2219 2230 2972 3161 2219
Size: 11 742 189 5065
Fragments arranged by size:
5065 742 189 11
PspXI vC'TCGA_Gb
Cuts at: 2438 2438
Size: 6007
PstI C_TGCA'G
Cuts at: 830 2382 2391 2607 2694 2815 3359 830
Size: 1552 9 216 87 121 544 3478
Fragments arranged by size:
3478 1552 544 216 121 87 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6007
PvuII CAG'CTG
Cuts at: 2269 2317 2383 3100 2269
Size: 48 66 717 5176
Fragments arranged by size:
5176 717 66 48
SacII CC_GC'GG
Cuts at: 2808 2979 2808
Size: 171 5836
SalI G'TCGA_C
Cuts at: 1936 3349 1936
Size: 1413 4594
SbfI CC_TGCA'GG
Cuts at: 2694 3359 2694
Size: 665 5342
ScaI AGT'ACT
Cuts at: 4255 5967 4255
Size: 1712 4295
SfcI C'TryA_G
Cuts at: 826 1045 2378 2387 2603 2690 2811 3355 4490
Size: 219 1333 9 216 87 121 544 1135
Cuts at: 4490 5171 5362 826
Size: 681 191 1471
Fragments arranged by size:
1471 1333 1135 681 544 219 216 191
121 87 9
SfoI GGC'GCC
Cuts at: 2043 2407 2043
Size: 364 5643
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6007
SgrAI Cr'CCGG_yG
Cuts at: 1700 2038 2445 1700
Size: 338 407 5262
Fragments arranged by size:
5262 407 338
SmaI CCC'GGG
Cuts at: 1872 3167 3334 1872
Size: 1295 167 4545
Fragments arranged by size:
4545 1295 167
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2438 4111 5012 5274 820
Size: 197 926 495 1673 901 262 1553
Fragments arranged by size:
1673 1553 926 901 495 262 197
SnaBI TAC'GTA
Cuts at: 493 3798 493
Size: 3305 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6007
SphI G_CATG'C
Cuts at: 1132 3365 1132
Size: 2233 3774
SspI AAT'ATT
Cuts at: 5 52 3931 5
Size: 47 3879 2081
Fragments arranged by size:
3879 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6007
StyI C'CwwG_G
Cuts at: 513 1065 2004 2091 2871 3446 513
Size: 552 939 87 780 575 3074
Fragments arranged by size:
3074 939 780 575 552 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4034 4051 4204 4389 4834 325
Size: 1255 2454 17 153 185 445 1498
Fragments arranged by size:
2454 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4253 5965 96
Size: 274 80 33 51 1443 2276 1712 138
Fragments arranged by size:
2276 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2351 2383 2756 2788 5967 5999 2351
Size: 32 373 32 3179 32 2359
Fragments arranged by size:
3179 2359 373 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6007
XbaI T'CTAG_A
Cuts at: 3343 3343
Size: 6007
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2359 2359
Size: 6007
XhoI C'TCGA_G
Cuts at: 1943 2438 1943
Size: 495 5512
XmaI C'CCGG_G
Cuts at: 1870 3165 3332 1870
Size: 1295 167 4545
Fragments arranged by size:
4545 1295 167
XmnI GAAnn'nnTTC
Cuts at: 4136 4136
Size: 6007
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3815 276
Size: 53 83 186 936 2281 2468
Fragments arranged by size:
2468 2281 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SacII SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI
BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FspAI NruI
NsiI PacI PfoI PmlI PsrI RsrII SacI SanDI
SapI SexAI SfiI SrfI SwaI