Restriction map for
pFN21AE0638
(Circular) (Six-base) MAPSORT of: pFN21AE0638.seq Check: 6226 from: 1 to: 6046
pFN21AE0638 6046 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3856 278
Size: 53 83 186 936 2320 2468
Fragments arranged by size:
2468 2320 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3171 3389 1937
Size: 1234 218 4594
Fragments arranged by size:
4594 1234 218
Acc65I G'GTAC_C
Cuts at: 3367 3367
Size: 6046
AclI AA'CG_TT
Cuts at: 1563 4173 4546 1563
Size: 2610 373 3063
Fragments arranged by size:
3063 2610 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4109 5493 5761 1632
Size: 2477 1384 268 1917
Fragments arranged by size:
2477 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5849
AflIII A'CryG_T
Cuts at: 1203 3063 4945 5922 1203
Size: 1860 1882 977 1327
Fragments arranged by size:
1882 1860 1327 977
AgeI A'CCGG_T
Cuts at: 4877 4877
Size: 6046
AhdI GACnn_n'nnGTC
Cuts at: 4775 4775
Size: 6046
AleI CACnn'nnGTG
Cuts at: 1166 2494 2585 1166
Size: 1328 91 4627
Fragments arranged by size:
4627 1328 91
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6046
AlwNI CAG_nnn'CTG
Cuts at: 2819 3789 5361 2819
Size: 970 1572 3504
Fragments arranged by size:
3504 1572 970
ApaI G_GGCC'C
Cuts at: 3341 3341
Size: 6046
ApaLI G'TGCA_C
Cuts at: 3090 4103 5259 3090
Size: 1013 1156 3877
Fragments arranged by size:
3877 1156 1013
ApoI r'AATT_y
Cuts at: 1493 3355 3581 3733 5842 1493
Size: 1862 226 152 2109 1697
Fragments arranged by size:
2109 1862 1697 226 152
AscI GG'CGCG_CC
Cuts at: 3070 3070
Size: 6046
AseI AT'TA_AT
Cuts at: 160 1032 4600 160
Size: 872 3568 1606
Fragments arranged by size:
3568 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6046
AvaI C'yCGr_G
Cuts at: 1870 1943 2048 2074 2692 3085 3106 3371 1870
Size: 73 105 26 618 393 21 265 4545
Fragments arranged by size:
4545 618 393 265 105 73 26 21
BamHI G'GATC_C
Cuts at: 2948 3376 3743 2948
Size: 428 367 5251
Fragments arranged by size:
5251 428 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2080 2372 2563 3367 4822
Size: 325 217 627 293 292 191 804 1455
Cuts at: 4822 618
Size: 1842
Fragments arranged by size:
1842 1455 804 627 325 293 292 217
191
BanII G_rGCy'C
Cuts at: 1391 2127 2739 3250 3262 3341 3365 1391
Size: 736 612 511 12 79 24 4072
Fragments arranged by size:
4072 736 612 511 79 24 12
BbeI G_GCGC'C
Cuts at: 2084 2084
Size: 6046
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2874 928
Size: 971 975 4100
Fragments arranged by size:
4100 975 971
BbvCI CC'TCA_GC
Cuts at: 2114 2114
Size: 6046
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4237 4271 1903
Size: 34 2300 34 3678
Fragments arranged by size:
3678 2300 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3938 5148 3938
Size: 1210 4836
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6046
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4657 136
Size: 107 122 71 631 605 2985 1525
Fragments arranged by size:
2985 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6041 6041
Size: 6046
BlpI GC'TnA_GC
Cuts at: 2432 3137 3311 3463 2432
Size: 705 174 152 5015
Fragments arranged by size:
5015 705 174 152
Bme1580I G_kGCm'C
Cuts at: 1790 2360 2568 3094 3341 4107 5263 1790
Size: 570 208 526 247 766 1156 2573
Fragments arranged by size:
2573 1156 766 570 526 247 208
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6046
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2628 3344 3777
Size: 416 53 83 399 24 1211 716 433
Cuts at: 3777 4735 442
Size: 958 1753
Fragments arranged by size:
1753 1211 958 716 433 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6046
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2474 2936 4706 1239
Size: 1235 462 1770 2579
Fragments arranged by size:
2579 1770 1235 462
Bpu10I CC'TnA_GC
Cuts at: 2114 2114
Size: 6046
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4171 5036 5334 4171
Size: 865 298 4883
Fragments arranged by size:
4883 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4709 882
Size: 617 3210 2219
Fragments arranged by size:
3210 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2538 2887 3066 3837 493
Size: 711 1334 349 179 771 2702
Fragments arranged by size:
2702 1334 771 711 349 179
BsaBI GATnn'nnATC
Cuts at: 6040 6040
Size: 6046
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2081 3853 4235 4870
Size: 53 83 186 936 548 1772 382 635
Cuts at: 4870 275
Size: 1451
Fragments arranged by size:
1772 1451 936 635 548 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2025 2042 2549 4479 4877 5151 5298 5709 2025
Size: 17 507 1930 398 274 147 411 2362
Fragments arranged by size:
2362 1930 507 411 398 274 147 17
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5821
Fragments arranged by size:
5821 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2078 2273 1186
Size: 892 195 4959
Fragments arranged by size:
4959 892 195
BseYI C'CCAG_C
Cuts at: 5249 5745 5249
Size: 496 5550
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2054 1163
Size: 573 318 5155
Fragments arranged by size:
5155 573 318
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3496 4257 5285 1540
Size: 275 186 1495 761 1028 2301
Fragments arranged by size:
2301 1495 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2739 3094 3103 3115 3250 4107 4192 5263 2739
Size: 355 9 12 135 857 85 1071 3522
Fragments arranged by size:
3522 1071 857 355 135 85 12 9
BsmI GAATG_Cn'
Cuts at: 2133 3557 3650 2133
Size: 1424 93 4529
Fragments arranged by size:
4529 1424 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2127 2360 2568 2739 3094 3103 3115
Size: 399 337 233 208 171 355 9 12
Cuts at: 3115 3250 3262 3341 3365 4107 4192 5263 1391
Size: 135 12 79 24 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 355 337 233 208
171 135 85 79 24 12 12 9
BspEI T'CCGG_A
Cuts at: 2549 5709 2549
Size: 3160 2886
BspHI T'CATG_A
Cuts at: 3935 5665 3935
Size: 1730 4316
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3401 844
Size: 2557 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2458 3933 1394
Size: 429 635 1475 3507
Fragments arranged by size:
3507 1475 635 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3291 4541 4715 65
Size: 1183 2043 1250 174 1396
Fragments arranged by size:
2043 1396 1250 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2841 4690 4877 1700
Size: 110 1031 1849 187 2869
Fragments arranged by size:
2869 1849 1031 187 110
BsrGI T'GTAC_A
Cuts at: 96 2577 96
Size: 2481 3565
BssHII G'CGCG_C
Cuts at: 1925 3070 1925
Size: 1145 4901
BssSI C'ACGA_G
Cuts at: 2151 2438 2490 2604 3243 4106 5118 2151
Size: 287 52 114 639 863 1012 3079
Fragments arranged by size:
3079 1012 863 639 287 114 52
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2620 794
Size: 1826 4220
BstBI TT'CG_AA
Cuts at: 4850 4850
Size: 6046
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6046
BstXI CCAn_nnnn'nTGG
Cuts at: 2353 2452 2980 2353
Size: 99 528 5419
Fragments arranged by size:
5419 528 99
BstYI r'GATC_y
Cuts at: 1972 2948 3376 3743 4128 4145 5597 5683 5695
Size: 976 428 367 385 17 1452 86 12
Cuts at: 5695 6041 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 976 428 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2258 2258
Size: 6046
BtgI C'CryG_G
Cuts at: 513 1065 2004 2709 513
Size: 552 939 705 3850
Fragments arranged by size:
3850 939 705 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2431 2609 2889 487
Size: 683 56 1205 178 280 3644
Fragments arranged by size:
3644 1205 683 280 178 56
BtsI GCAGTG_nn'
Cuts at: 2107 2196 2480 2621 3562 4356 4376 2107
Size: 89 284 141 941 794 20 3777
Fragments arranged by size:
3777 941 794 284 141 89 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6046
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6011
DraI TTT'AAA
Cuts at: 3350 3350
Size: 6046
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5053 809
Size: 507 3737 1802
Fragments arranged by size:
3737 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2166 2442 2706
Size: 54 1425 50 275 18 336 276 264
Cuts at: 2706 3493 4382 6011 8
Size: 787 889 1629 43
Fragments arranged by size:
1629 1425 889 787 336 276 275 264
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3493 1537
Size: 275 1681 4090
Fragments arranged by size:
4090 1681 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3267 3976 1353
Size: 1914 709 3423
Fragments arranged by size:
3423 1914 709
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2636 4633 5007 5153 387
Size: 1064 138 1047 1997 374 146 1280
Fragments arranged by size:
1997 1280 1064 1047 374 146 138
EcoICRI GAG'CTC
Cuts at: 2737 3248 2737
Size: 511 5535
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2474 2936 4109 4706 5493 5761 1239
Size: 393 842 462 1173 597 787 268 1524
Fragments arranged by size:
1524 1173 842 787 597 462 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2600 3274 3338 2600
Size: 674 64 5308
Fragments arranged by size:
5308 674 64
EcoRI G'AATT_C
Cuts at: 3355 3733 3355
Size: 378 5668
FspI TGC'GCA
Cuts at: 4552 4552
Size: 6046
HaeII r_GCGC'y
Cuts at: 1678 2084 2469 2930 5193 1678
Size: 406 385 461 2263 2531
Fragments arranged by size:
2531 2263 461 406 385
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4687 4719 4761 4793 1373
Size: 32 135 32 3115 32 42 32 2626
Fragments arranged by size:
3115 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3160 3390 3636 669
Size: 988 281 1222 230 246 3079
Fragments arranged by size:
3079 1222 988 281 246 230
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6046
HpaI GTT'AAC
Cuts at: 3636 3636
Size: 6046
KasI G'GCGC_C
Cuts at: 2080 2080
Size: 6046
KpnI G_GTAC'C
Cuts at: 3371 3371
Size: 6046
MfeI C'AATT_G
Cuts at: 3645 3645
Size: 6046
MluI A'CGCG_T
Cuts at: 5922 5922
Size: 6046
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2191 5160 5344 1305
Size: 886 2969 184 2007
Fragments arranged by size:
2969 2007 886 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2168 2444 10
Size: 54 1425 343 336 276 3612
Fragments arranged by size:
3612 1425 343 336 276 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2231 2408 2494 2585 4004 4363 4522
Size: 648 1065 177 86 91 1419 359 159
Cuts at: 4522 518
Size: 2042
Fragments arranged by size:
2042 1419 1065 648 359 177 159 91
86
MspA1I CmG'CkG
Cuts at: 1606 2118 3462 4139 5287 5532 1606
Size: 512 1344 677 1148 245 2120
Fragments arranged by size:
2120 1344 1148 677 512 245
NaeI GCC'GGC
Cuts at: 2843 2843
Size: 6046
NarI GG'CG_CC
Cuts at: 2081 2081
Size: 6046
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4555
Fragments arranged by size:
4555 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6046
NgoMIV G'CCGG_C
Cuts at: 2841 2841
Size: 6046
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6046
NotI GC'GGCC_GC
Cuts at: 3493 3493
Size: 6046
NruI TCG'CGA
Cuts at: 2733 2733
Size: 6046
NspI r_CATG'y
Cuts at: 1132 1229 2329 3404 4949 1132
Size: 97 1100 1075 1545 2229
Fragments arranged by size:
2229 1545 1100 1075 97
PasI CC'CwG_GG
Cuts at: 1348 3255 1348
Size: 1907 4139
PciI A'CATG_T
Cuts at: 4945 4945
Size: 6046
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3066 1108
Size: 1958 4088
PmeI GTTT'AAAC
Cuts at: 3350 3350
Size: 6046
PmlI CAC'GTG
Cuts at: 2538 3066 2538
Size: 528 5518
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4115 4147 5628 5660 4115
Size: 32 1481 32 4501
Fragments arranged by size:
4501 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3274 3274
Size: 6046
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6046
PsiI TTA'TAA
Cuts at: 3616 3616
Size: 6046
PspOMI G'GGCC_C
Cuts at: 3337 3337
Size: 6046
PstI C_TGCA'G
Cuts at: 830 2487 2616 3398 830
Size: 1657 129 782 3478
Fragments arranged by size:
3478 1657 782 129
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6046
PvuII CAG'CTG
Cuts at: 2118 2118
Size: 6046
SacI G_AGCT'C
Cuts at: 2739 3250 2739
Size: 511 5535
SalI G'TCGA_C
Cuts at: 1936 3388 1936
Size: 1452 4594
SapI GCTCTTCn'nnn_
Cuts at: 3267 3267
Size: 6046
SbfI CC_TGCA'GG
Cuts at: 3398 3398
Size: 6046
ScaI AGT'ACT
Cuts at: 4294 6006 4294
Size: 1712 4334
SexAI A'CCwGG_T
Cuts at: 2654 2654
Size: 6046
SfcI C'TryA_G
Cuts at: 826 1045 2483 2612 3394 4529 5210 5401 826
Size: 219 1438 129 782 1135 681 191 1471
Fragments arranged by size:
1471 1438 1135 782 681 219 191 129
SfoI GGC'GCC
Cuts at: 2082 2082
Size: 6046
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6046
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6046
SmaI CCC'GGG
Cuts at: 1872 2050 2076 2694 3087 3373 1872
Size: 178 26 618 393 286 4545
Fragments arranged by size:
4545 618 393 286 178 26
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4150 5051 5313 820
Size: 197 926 2207 901 262 1553
Fragments arranged by size:
2207 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3837 493
Size: 3344 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6046
SphI G_CATG'C
Cuts at: 1132 2329 3404 1132
Size: 1197 1075 3774
Fragments arranged by size:
3774 1197 1075
SspI AAT'ATT
Cuts at: 5 52 3970 5
Size: 47 3918 2081
Fragments arranged by size:
3918 2081 47
StuI AGG'CCT
Cuts at: 1517 3203 1517
Size: 1686 4360
StyI C'CwwG_G
Cuts at: 513 1065 2004 2800 3485 513
Size: 552 939 796 685 3074
Fragments arranged by size:
3074 939 796 685 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2981 4073 4090 4243 4428 4873 325
Size: 1255 1401 1092 17 153 185 445 1498
Fragments arranged by size:
1498 1401 1255 1092 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2577 4292 6004
Size: 274 80 33 51 1443 600 1715 1712
Cuts at: 6004 96
Size: 138
Fragments arranged by size:
1715 1712 1443 600 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6006 6038 6006
Size: 32 6014
Tth111I GACn'n_nGTC
Cuts at: 1316 2656 1316
Size: 1340 4706
XbaI T'CTAG_A
Cuts at: 3382 3382
Size: 6046
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3331 3331
Size: 6046
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6046
XmaI C'CCGG_G
Cuts at: 1870 2048 2074 2692 3085 3371 1870
Size: 178 26 618 393 286 4545
Fragments arranged by size:
4545 618 393 286 178 26
XmnI GAAnn'nnTTC
Cuts at: 4175 4175
Size: 6046
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3854 276
Size: 53 83 186 936 2320 2468
Fragments arranged by size:
2468 2320 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI
NsiI PacI PfoI PspXI PsrI RsrII SacII SanDI
SfiI SrfI SwaI