Restriction map for
pFN21AE0637
(Circular) (Six-base) MAPSORT of: pFN21AE0637.seq Check: 495 from: 1 to: 5332
pFN21AE0637 5332 bp
With 182 enzymes: SgfI *
October 20, 2009 10:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3142 278
Size: 53 83 186 936 1606 2468
Fragments arranged by size:
2468 1606 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2675 1937
Size: 738 4594
Acc65I G'GTAC_C
Cuts at: 2580 2653 2580
Size: 73 5259
AclI AA'CG_TT
Cuts at: 1563 3459 3832 1563
Size: 1896 373 3063
Fragments arranged by size:
3063 1896 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2036 3395 4779 5047 1632
Size: 404 1359 1384 268 1917
Fragments arranged by size:
1917 1384 1359 404 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5135
AflIII A'CryG_T
Cuts at: 1203 4231 5208 1203
Size: 3028 977 1327
Fragments arranged by size:
3028 1327 977
AgeI A'CCGG_T
Cuts at: 4163 4163
Size: 5332
AhdI GACnn_n'nnGTC
Cuts at: 4061 4061
Size: 5332
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5332
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5332
AlwNI CAG_nnn'CTG
Cuts at: 2305 3075 4647 2305
Size: 770 1572 2990
Fragments arranged by size:
2990 1572 770
ApaI G_GGCC'C
Cuts at: 2473 2473
Size: 5332
ApaLI G'TGCA_C
Cuts at: 2381 3389 4545 2381
Size: 1008 1156 3168
Fragments arranged by size:
3168 1156 1008
ApoI r'AATT_y
Cuts at: 1493 2641 2867 3019 5128 1493
Size: 1148 226 152 2109 1697
Fragments arranged by size:
2109 1697 1148 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3886 160
Size: 872 2854 1606
Fragments arranged by size:
2854 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5332
AvaI C'yCGr_G
Cuts at: 1870 1943 2657 1870
Size: 73 714 4545
Fragments arranged by size:
4545 714 73
AvrII C'CTAG_G
Cuts at: 2392 2392
Size: 5332
BamHI G'GATC_C
Cuts at: 2662 3029 2662
Size: 367 4965
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2048 2075 2580 2653 4108
Size: 325 217 627 261 27 505 73 1455
Cuts at: 4108 618
Size: 1842
Fragments arranged by size:
1842 1455 627 505 325 261 217 73
27
BanII G_rGCy'C
Cuts at: 1391 2069 2473 2651 1391
Size: 678 404 178 4072
Fragments arranged by size:
4072 678 404 178
BbeI G_GCGC'C
Cuts at: 2079 2079
Size: 5332
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4361
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3523 3557 1903
Size: 34 1586 34 3678
Fragments arranged by size:
3678 1586 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3224 4434 3224
Size: 1210 4122
BclI T'GATC_A
Cuts at: 1548 2187 1548
Size: 639 4693
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3943 136
Size: 107 122 71 631 605 2271 1525
Fragments arranged by size:
2271 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5327 5327
Size: 5332
BlpI GC'TnA_GC
Cuts at: 2749 2749
Size: 5332
Bme1580I G_kGCm'C
Cuts at: 1790 2385 2473 3393 4549 1790
Size: 595 88 920 1156 2573
Fragments arranged by size:
2573 1156 920 595 88
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5332
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3063 4021 442
Size: 416 53 83 399 24 1646 958 1753
Fragments arranged by size:
1753 1646 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5332
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2102 3992 1239
Size: 863 1890 2579
Fragments arranged by size:
2579 1890 863
Bpu10I CC'TnA_GC
Cuts at: 2568 2568
Size: 5332
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3457 4322 4620 3457
Size: 865 298 4169
Fragments arranged by size:
4169 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3995 882
Size: 617 2496 2219
Fragments arranged by size:
2496 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3123 493
Size: 711 1919 2702
Fragments arranged by size:
2702 1919 711
BsaBI GATnn'nnATC
Cuts at: 5326 5326
Size: 5332
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2076 3139 3521 4156
Size: 53 83 186 936 543 1063 382 635
Cuts at: 4156 275
Size: 1451
Fragments arranged by size:
1451 1063 936 635 543 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2094 3765 4163 4437 4584 4995 2094
Size: 1671 398 274 147 411 2431
Fragments arranged by size:
2431 1671 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5107
Fragments arranged by size:
5107 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2099 2102 1186
Size: 913 3 4416
Fragments arranged by size:
4416 913 3
BseYI C'CCAG_C
Cuts at: 2372 2503 2601 4535 5031 2372
Size: 131 98 1934 496 2673
Fragments arranged by size:
2673 1934 496 131 98
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2259 1163
Size: 573 523 4236
Fragments arranged by size:
4236 573 523
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2162 2782 3543 4571 1540
Size: 275 186 161 620 761 1028 2301
Fragments arranged by size:
2301 1028 761 620 275 186 161
BsiHKAI G_wGCw'C
Cuts at: 2034 2385 2401 3393 3478 4549 2034
Size: 351 16 992 85 1071 2817
Fragments arranged by size:
2817 1071 992 351 85 16
BsmI GAATG_Cn'
Cuts at: 2266 2843 2936 2266
Size: 577 93 4662
Fragments arranged by size:
4662 577 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2034 2069 2385 2401 2473 2651 3393
Size: 399 244 35 316 16 72 178 742
Cuts at: 3393 3478 4549 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 316 244 178 85
72 35 16
BspEI T'CCGG_A
Cuts at: 2094 4995 2094
Size: 2901 2431
BspHI T'CATG_A
Cuts at: 3221 4951 3221
Size: 1730 3602
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2687 844
Size: 1843 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3219 1394
Size: 429 1396 3507
Fragments arranged by size:
3507 1396 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3827 4001 65
Size: 1183 2579 174 1396
Fragments arranged by size:
2579 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2045 2051 3976 4163 1700
Size: 110 235 6 1925 187 2869
Fragments arranged by size:
2869 1925 235 187 110 6
BsrGI T'GTAC_A
Cuts at: 96 2484 96
Size: 2388 2944
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5332
BssSI C'ACGA_G
Cuts at: 3392 4404 3392
Size: 1012 4320
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5332
BstBI TT'CG_AA
Cuts at: 4136 4136
Size: 5332
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5332
BstXI CCAn_nnnn'nTGG
Cuts at: 2466 2466
Size: 5332
BstYI r'GATC_y
Cuts at: 1972 2662 3029 3414 3431 4883 4969 4981 5327
Size: 690 367 385 17 1452 86 12 346
Cuts at: 5327 1972
Size: 1977
Fragments arranged by size:
1977 1452 690 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4780
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2392 487
Size: 683 56 1166 3427
Fragments arranged by size:
3427 1166 683 56
BtsI GCAGTG_nn'
Cuts at: 2196 2541 2848 3642 3662 2196
Size: 345 307 794 20 3866
Fragments arranged by size:
3866 794 345 307 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5332
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5297
DraI TTT'AAA
Cuts at: 2636 2636
Size: 5332
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4339 809
Size: 507 3023 1802
Fragments arranged by size:
3023 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2159 2253 2340
Size: 54 1425 50 275 18 329 94 87
Cuts at: 2340 2779 3668 5297 8
Size: 439 889 1629 43
Fragments arranged by size:
1629 1425 889 439 329 275 94 87
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2159 2779 1537
Size: 275 347 620 4090
Fragments arranged by size:
4090 620 347 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2159 3262 1353
Size: 806 1103 3423
Fragments arranged by size:
3423 1103 806
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3919 4293 4439 387
Size: 1064 138 2330 374 146 1280
Fragments arranged by size:
2330 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2036 2102 3395 3992 4779 5047 1239
Size: 393 404 66 1293 597 787 268 1524
Fragments arranged by size:
1524 1293 787 597 404 393 268 66
EcoNI CCTnn'n_nnAGG
Cuts at: 2084 2084
Size: 5332
EcoO109I rG'GnC_Cy
Cuts at: 2181 2416 2470 2181
Size: 235 54 5043
Fragments arranged by size:
5043 235 54
EcoRI G'AATT_C
Cuts at: 2641 3019 2641
Size: 378 4954
FspI TGC'GCA
Cuts at: 3838 3838
Size: 5332
HaeII r_GCGC'y
Cuts at: 1678 2079 4479 1678
Size: 401 2400 2531
Fragments arranged by size:
2531 2400 401
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3973 4005 4047 4079 1373
Size: 32 135 32 2401 32 42 32 2626
Fragments arranged by size:
2626 2401 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2676 2922 669
Size: 988 281 738 246 3079
Fragments arranged by size:
3079 988 738 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5332
HpaI GTT'AAC
Cuts at: 2922 2922
Size: 5332
KasI G'GCGC_C
Cuts at: 2075 2075
Size: 5332
KpnI G_GTAC'C
Cuts at: 2584 2657 2584
Size: 73 5259
MfeI C'AATT_G
Cuts at: 2931 2931
Size: 5332
MluI A'CGCG_T
Cuts at: 5208 5208
Size: 5332
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4446 4630 1305
Size: 3141 184 2007
Fragments arranged by size:
3141 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2342 10
Size: 54 1425 343 510 3000
Fragments arranged by size:
3000 1425 510 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2336 3290 3649 3808 518
Size: 648 1170 954 359 159 2042
Fragments arranged by size:
2042 1170 954 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2112 2549 2748 3425 4573 4818 1606
Size: 506 437 199 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 506 437 245 199
NarI GG'CG_CC
Cuts at: 2076 2076
Size: 5332
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4780
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5332
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5332
NotI GC'GGCC_GC
Cuts at: 2779 2779
Size: 5332
NspI r_CATG'y
Cuts at: 1132 1229 2690 4235 1132
Size: 97 1461 1545 2229
Fragments arranged by size:
2229 1545 1461 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5332
PciI A'CATG_T
Cuts at: 4231 4231
Size: 5332
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5332
PmeI GTTT'AAAC
Cuts at: 2636 2636
Size: 5332
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3401 3433 4914 4946 3401
Size: 32 1481 32 3787
Fragments arranged by size:
3787 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2181 2416 2181
Size: 235 5097
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5332
PsiI TTA'TAA
Cuts at: 2902 2902
Size: 5332
PspOMI G'GGCC_C
Cuts at: 2469 2469
Size: 5332
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2609 2641 2609
Size: 32 5300
PstI C_TGCA'G
Cuts at: 830 2684 830
Size: 1854 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5332
SalI G'TCGA_C
Cuts at: 1936 2674 1936
Size: 738 4594
SanDI GG'GwC_CC
Cuts at: 2181 2416 2181
Size: 235 5097
SapI GCTCTTCn'nnn_
Cuts at: 2159 2159
Size: 5332
SbfI CC_TGCA'GG
Cuts at: 2684 2684
Size: 5332
ScaI AGT'ACT
Cuts at: 3580 5292 3580
Size: 1712 3620
SfcI C'TryA_G
Cuts at: 826 1045 2680 3815 4496 4687 826
Size: 219 1635 1135 681 191 1471
Fragments arranged by size:
1635 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2077 2077
Size: 5332
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5332
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5332
SmaI CCC'GGG
Cuts at: 1872 2659 1872
Size: 787 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3436 4337 4599 820
Size: 197 926 1493 901 262 1553
Fragments arranged by size:
1553 1493 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3123 493
Size: 2630 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5332
SphI G_CATG'C
Cuts at: 1132 2690 1132
Size: 1558 3774
SspI AAT'ATT
Cuts at: 5 52 3256 5
Size: 47 3204 2081
Fragments arranged by size:
3204 2081 47
StuI AGG'CCT
Cuts at: 1517 2465 1517
Size: 948 4384
StyI C'CwwG_G
Cuts at: 513 1065 2392 2771 513
Size: 552 1327 379 3074
Fragments arranged by size:
3074 1327 552 379
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2615 3359 3376 3529 3714 4159 325
Size: 1255 1035 744 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1035 744 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2484 3578 5290
Size: 274 80 33 51 1443 507 1094 1712
Cuts at: 5290 96
Size: 138
Fragments arranged by size:
1712 1443 1094 507 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5292 5324 5292
Size: 32 5300
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5332
XbaI T'CTAG_A
Cuts at: 2668 2668
Size: 5332
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2335 2335
Size: 5332
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5332
XmaI C'CCGG_G
Cuts at: 1870 2657 1870
Size: 787 4545
XmnI GAAnn'nnTTC
Cuts at: 3461 3461
Size: 5332
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3140 276
Size: 53 83 186 936 1606 2468
Fragments arranged by size:
2468 1606 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PsrI PstI PvuI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbvCI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PmlI PspXI PvuII RsrII SacI SacII SexAI SfiI
SrfI SwaI