Restriction map for
pFN21AE0632
(Circular) (Six-base) MAPSORT of: pFN21AE0632.seq Check: 2381 from: 1 to: 5833
pFN21AE0632 5833 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2907 3643 278
Size: 53 83 186 936 1371 736 2468
Fragments arranged by size:
2468 1371 936 736 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3176 1937
Size: 1239 4594
Acc65I G'GTAC_C
Cuts at: 2599 3154 2599
Size: 555 5278
AclI AA'CG_TT
Cuts at: 1563 3960 4333 1563
Size: 2397 373 3063
Fragments arranged by size:
3063 2397 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3896 5280 5548 1632
Size: 2264 1384 268 1917
Fragments arranged by size:
2264 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5636
AflIII A'CryG_T
Cuts at: 1203 4732 5709 1203
Size: 3529 977 1327
Fragments arranged by size:
3529 1327 977
AgeI A'CCGG_T
Cuts at: 4664 4664
Size: 5833
AhdI GACnn_n'nnGTC
Cuts at: 4562 4562
Size: 5833
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5833
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2645 1184
Size: 1461 4372
AlwNI CAG_nnn'CTG
Cuts at: 3007 3046 3576 5148 3007
Size: 39 530 1572 3692
Fragments arranged by size:
3692 1572 530 39
ApaI G_GGCC'C
Cuts at: 3120 3120
Size: 5833
ApaLI G'TGCA_C
Cuts at: 2153 2204 2391 3890 5046 2153
Size: 51 187 1499 1156 2940
Fragments arranged by size:
2940 1499 1156 187 51
ApoI r'AATT_y
Cuts at: 1493 2087 3142 3368 3520 5629 1493
Size: 594 1055 226 152 2109 1697
Fragments arranged by size:
2109 1697 1055 594 226 152
AscI GG'CGCG_CC
Cuts at: 2955 2955
Size: 5833
AseI AT'TA_AT
Cuts at: 160 1032 4387 160
Size: 872 3355 1606
Fragments arranged by size:
3355 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5833
AvaI C'yCGr_G
Cuts at: 1870 1943 2462 2603 2800 2950 2976 3158 1870
Size: 73 519 141 197 150 26 182 4545
Fragments arranged by size:
4545 519 197 182 150 141 73 26
BamHI G'GATC_C
Cuts at: 3163 3530 3163
Size: 367 5466
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2534 2572 2599 2819 3154
Size: 325 217 627 747 38 27 220 335
Cuts at: 3154 4609 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 747 627 335 325 220 217
38 27
BanII G_rGCy'C
Cuts at: 1391 2333 3120 3152 1391
Size: 942 787 32 4072
Fragments arranged by size:
4072 942 787 32
BbeI G_GCGC'C
Cuts at: 2538 2538
Size: 5833
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2275 2690 928
Size: 971 376 415 4071
Fragments arranged by size:
4071 971 415 376
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4024 4058 1903
Size: 34 2087 34 3678
Fragments arranged by size:
3678 2087 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3725 4935 3725
Size: 1210 4623
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5833
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4444 136
Size: 107 122 71 631 605 2772 1525
Fragments arranged by size:
2772 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5828 5828
Size: 5833
BlpI GC'TnA_GC
Cuts at: 3050 3250 3050
Size: 200 5633
Bme1580I G_kGCm'C
Cuts at: 1790 2157 2208 2395 2577 2921 3120 3894 5050
Size: 367 51 187 182 344 199 774 1156
Cuts at: 5050 1790
Size: 2573
Fragments arranged by size:
2573 1156 774 367 344 199 187 182
51
BmgBI CAC'GTC
Cuts at: 1318 2936 1318
Size: 1618 4215
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3564 4522 442
Size: 416 53 83 399 24 2147 958 1753
Fragments arranged by size:
2147 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5833
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2585 2675 2945 3023 4493 1239
Size: 1346 90 270 78 1470 2579
Fragments arranged by size:
2579 1470 1346 270 90 78
Bpu10I CC'TnA_GC
Cuts at: 2276 2276
Size: 5833
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3958 4823 5121 3958
Size: 865 298 4670
Fragments arranged by size:
4670 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2205 4496 882
Size: 617 706 2291 2219
Fragments arranged by size:
2291 2219 706 617
BsaAI yAC'GTr
Cuts at: 493 1204 3624 493
Size: 711 2420 2702
Fragments arranged by size:
2702 2420 711
BsaBI GATnn'nnATC
Cuts at: 5827 5827
Size: 5833
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2535 2904 3640 4022
Size: 53 83 186 936 1002 369 736 382
Cuts at: 4022 4657 275
Size: 635 1451
Fragments arranged by size:
1451 1002 936 736 635 382 369 186
83 53
BsaWI w'CCGG_w
Cuts at: 4266 4664 4938 5085 5496 4266
Size: 398 274 147 411 4603
Fragments arranged by size:
4603 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2153 2183 1178
Size: 30 165 30 750 30 4828
Fragments arranged by size:
4828 750 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2315 3122 1186
Size: 1129 807 3897
Fragments arranged by size:
3897 1129 807
BseYI C'CCAG_C
Cuts at: 2356 2512 5036 5532 2356
Size: 156 2524 496 2657
Fragments arranged by size:
2657 2524 496 156
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2359 2471 2916 1163
Size: 573 623 112 445 4080
Fragments arranged by size:
4080 623 573 445 112
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2785 3283 4044 5072 1540
Size: 275 186 784 498 761 1028 2301
Fragments arranged by size:
2301 1028 784 761 498 275 186
BsiHKAI G_wGCw'C
Cuts at: 2157 2208 2395 2624 3114 3894 3979 5050 2157
Size: 51 187 229 490 780 85 1071 2940
Fragments arranged by size:
2940 1071 780 490 229 187 85 51
BsmI GAATG_Cn'
Cuts at: 3344 3437 3344
Size: 93 5740
BsmBI CGTCTCn'nnnn_
Cuts at: 2459 2459
Size: 5833
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2157 2208 2333 2395 2577 2624 2921
Size: 399 367 51 125 62 182 47 297
Cuts at: 2921 3114 3120 3152 3894 3979 5050 1391
Size: 193 6 32 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 367 297 193 182
125 85 62 51 47 32 6
BspEI T'CCGG_A
Cuts at: 5496 5496
Size: 5833
BspHI T'CATG_A
Cuts at: 3722 5452 3722
Size: 1730 4103
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2223 3188 844
Size: 1379 965 3489
Fragments arranged by size:
3489 1379 965
BsrBI CCG'CTC
Cuts at: 1394 1823 3720 1394
Size: 429 1897 3507
Fragments arranged by size:
3507 1897 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4328 4502 65
Size: 1183 3080 174 1396
Fragments arranged by size:
3080 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2472 2490 4477 4664 1700
Size: 110 662 18 1987 187 2869
Fragments arranged by size:
2869 1987 662 187 110 18
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5833
BssHII G'CGCG_C
Cuts at: 1925 2361 2955 1925
Size: 436 594 4803
Fragments arranged by size:
4803 594 436
BssSI C'ACGA_G
Cuts at: 3893 4905 3893
Size: 1012 4821
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3007 794
Size: 2213 3620
BstBI TT'CG_AA
Cuts at: 4637 4637
Size: 5833
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5833
BstYI r'GATC_y
Cuts at: 1972 3163 3530 3915 3932 5384 5470 5482 5828
Size: 1191 367 385 17 1452 86 12 346
Cuts at: 5828 1972
Size: 1977
Fragments arranged by size:
1977 1452 1191 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2823 3120 513
Size: 552 1758 297 3226
Fragments arranged by size:
3226 1758 552 297
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2544 2904 487
Size: 683 56 1318 360 3416
Fragments arranged by size:
3416 1318 683 360 56
BtsI GCAGTG_nn'
Cuts at: 2064 3349 4143 4163 2064
Size: 1285 794 20 3734
Fragments arranged by size:
3734 1285 794 20
ClaI AT'CG_AT
Cuts at: 1554 2445 1554
Size: 891 4942
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5798
DraI TTT'AAA
Cuts at: 3137 3137
Size: 5833
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2903 4840 809
Size: 507 1587 1937 1802
Fragments arranged by size:
1937 1802 1587 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2676 2782 3280
Size: 54 1425 50 275 18 846 106 498
Cuts at: 3280 4169 5798 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 846 498 275 106 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2782 3280 1537
Size: 275 970 498 4090
Fragments arranged by size:
4090 970 498 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3763 1353
Size: 2410 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2099 2359 2414 2630 2897 4420
Size: 1064 138 510 260 55 216 267 1523
Cuts at: 4420 4794 4940 387
Size: 374 146 1280
Fragments arranged by size:
1523 1280 1064 510 374 267 260 216
146 138 55
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2585 2675 2945 3023 3896 4493 5280
Size: 393 953 90 270 78 873 597 787
Cuts at: 5280 5548 1239
Size: 268 1524
Fragments arranged by size:
1524 953 873 787 597 393 270 268
90 78
EcoO109I rG'GnC_Cy
Cuts at: 2378 2608 2650 2837 3016 2378
Size: 230 42 187 179 5195
Fragments arranged by size:
5195 230 187 179 42
EcoRI G'AATT_C
Cuts at: 2087 3142 3520 2087
Size: 1055 378 4400
Fragments arranged by size:
4400 1055 378
FspI TGC'GCA
Cuts at: 4339 4339
Size: 5833
HaeII r_GCGC'y
Cuts at: 1678 2357 2538 3089 4980 1678
Size: 679 181 551 1891 2531
Fragments arranged by size:
2531 1891 679 551 181
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4474 4506 4548 4580 1373
Size: 32 135 32 2902 32 42 32 2626
Fragments arranged by size:
2902 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3177 3423 669
Size: 988 281 1239 246 3079
Fragments arranged by size:
3079 1239 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5833
HpaI GTT'AAC
Cuts at: 3423 3423
Size: 5833
KasI G'GCGC_C
Cuts at: 2534 2534
Size: 5833
KpnI G_GTAC'C
Cuts at: 2603 3158 2603
Size: 555 5278
MfeI C'AATT_G
Cuts at: 3432 3432
Size: 5833
MluI A'CGCG_T
Cuts at: 5709 5709
Size: 5833
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2196 4947 5131 1305
Size: 891 2751 184 2007
Fragments arranged by size:
2751 2007 891 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4011
Fragments arranged by size:
4011 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2447 2672 2705 3791 4150 4309 518
Size: 648 1281 225 33 1086 359 159 2042
Fragments arranged by size:
2042 1281 1086 648 359 225 159 33
MspA1I CmG'CkG
Cuts at: 1606 2512 2825 3004 3013 3043 3122 3249 3926
Size: 906 313 179 9 30 79 127 677
Cuts at: 3926 5074 5319 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 906 677 313 245 179 127
79 30 9
NarI GG'CG_CC
Cuts at: 2535 2535
Size: 5833
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5281
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5833
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5833
NotI GC'GGCC_GC
Cuts at: 2782 3280 2782
Size: 498 5335
NspI r_CATG'y
Cuts at: 1132 1229 3191 4736 1132
Size: 97 1962 1545 2229
Fragments arranged by size:
2229 1962 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5833
PciI A'CATG_T
Cuts at: 4732 4732
Size: 5833
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5833
PfoI T'CCnGG_A
Cuts at: 2652 2652
Size: 5833
PmeI GTTT'AAAC
Cuts at: 3137 3137
Size: 5833
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3902 3934 5415 5447 3902
Size: 32 1481 32 4288
Fragments arranged by size:
4288 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2378 2650 2837 3016 2378
Size: 272 187 179 5195
Fragments arranged by size:
5195 272 187 179
PshAI GACnn'nnGTC
Cuts at: 1613 3072 1613
Size: 1459 4374
PsiI TTA'TAA
Cuts at: 3403 3403
Size: 5833
PspOMI G'GGCC_C
Cuts at: 3116 3116
Size: 5833
PstI C_TGCA'G
Cuts at: 830 2862 3012 3185 830
Size: 2032 150 173 3478
Fragments arranged by size:
3478 2032 173 150
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5833
SacII CC_GC'GG
Cuts at: 2826 3123 2826
Size: 297 5536
SalI G'TCGA_C
Cuts at: 1936 3175 1936
Size: 1239 4594
SanDI GG'GwC_CC
Cuts at: 2837 2837
Size: 5833
SbfI CC_TGCA'GG
Cuts at: 3185 3185
Size: 5833
ScaI AGT'ACT
Cuts at: 4081 5793 4081
Size: 1712 4121
SexAI A'CCwGG_T
Cuts at: 2454 2694 2454
Size: 240 5593
SfcI C'TryA_G
Cuts at: 826 1045 2858 3008 3181 4316 4997 5188 826
Size: 219 1813 150 173 1135 681 191 1471
Fragments arranged by size:
1813 1471 1135 681 219 191 173 150
SfoI GGC'GCC
Cuts at: 2536 2536
Size: 5833
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5833
SgrAI Cr'CCGG_yG
Cuts at: 1700 2472 1700
Size: 772 5061
SmaI CCC'GGG
Cuts at: 1872 2605 2952 2978 3160 1872
Size: 733 347 26 182 4545
Fragments arranged by size:
4545 733 347 182 26
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3937 4838 5100 820
Size: 197 926 1994 901 262 1553
Fragments arranged by size:
1994 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3624 493
Size: 3131 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5833
SphI G_CATG'C
Cuts at: 1132 3191 1132
Size: 2059 3774
SspI AAT'ATT
Cuts at: 5 52 3757 5
Size: 47 3705 2081
Fragments arranged by size:
3705 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5833
StyI C'CwwG_G
Cuts at: 513 1065 3272 513
Size: 552 2207 3074
Fragments arranged by size:
3074 2207 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2174 3860 3877 4030 4215 4660 325
Size: 1255 594 1686 17 153 185 445 1498
Fragments arranged by size:
1686 1498 1255 594 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4079 5791 96
Size: 274 80 33 51 1443 2102 1712 138
Fragments arranged by size:
2102 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2153 2185 5793 5825 2153
Size: 32 3608 32 2161
Fragments arranged by size:
3608 2161 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2456 2648 2696 2906 1316
Size: 1140 192 48 210 4243
Fragments arranged by size:
4243 1140 210 192 48
XbaI T'CTAG_A
Cuts at: 3169 3169
Size: 5833
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2446 2446
Size: 5833
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5833
XmaI C'CCGG_G
Cuts at: 1870 2603 2950 2976 3158 1870
Size: 733 347 26 182 4545
Fragments arranged by size:
4545 733 347 182 26
XmnI GAAnn'nnTTC
Cuts at: 3962 3962
Size: 5833
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2905 3641 276
Size: 53 83 186 936 1371 736 2468
Fragments arranged by size:
2468 1371 936 736 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI
SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AvrII BaeI BbvCI BfrBI BplI
BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI PvuII RsrII SacI SapI SfiI
SrfI SwaI