Restriction map for
pFN21AE0629
(Circular) (Six-base) MAPSORT of: pFN21AE0629.seq Check: 6397 from: 1 to: 5299
pFN21AE0629 5299 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3109 278
Size: 53 83 186 936 1573 2468
Fragments arranged by size:
2468 1573 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2642 1937
Size: 705 4594
Acc65I G'GTAC_C
Cuts at: 2620 2620
Size: 5299
AclI AA'CG_TT
Cuts at: 1563 3426 3799 1563
Size: 1863 373 3063
Fragments arranged by size:
3063 1863 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3362 4746 5014 1632
Size: 1730 1384 268 1917
Fragments arranged by size:
1917 1730 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5102
AflIII A'CryG_T
Cuts at: 1203 2475 4198 5175 1203
Size: 1272 1723 977 1327
Fragments arranged by size:
1723 1327 1272 977
AgeI A'CCGG_T
Cuts at: 2528 4130 2528
Size: 1602 3697
AhdI GACnn_n'nnGTC
Cuts at: 4028 4028
Size: 5299
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5299
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5299
AlwNI CAG_nnn'CTG
Cuts at: 3042 4614 3042
Size: 1572 3727
ApaLI G'TGCA_C
Cuts at: 3356 4512 3356
Size: 1156 4143
ApoI r'AATT_y
Cuts at: 1493 2312 2588 2608 2834 2986 5095 1493
Size: 819 276 20 226 152 2109 1697
Fragments arranged by size:
2109 1697 819 276 226 152 20
AseI AT'TA_AT
Cuts at: 160 1032 3853 160
Size: 872 2821 1606
Fragments arranged by size:
2821 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5299
AvaI C'yCGr_G
Cuts at: 1870 1943 2624 1870
Size: 73 681 4545
Fragments arranged by size:
4545 681 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2509 2542 2509
Size: 33 5266
BamHI G'GATC_C
Cuts at: 2629 2996 2629
Size: 367 4932
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2016 2197 2531 2620 4075
Size: 325 217 627 229 181 334 89 1455
Cuts at: 4075 618
Size: 1842
Fragments arranged by size:
1842 1455 627 334 325 229 217 181
89
BanII G_rGCy'C
Cuts at: 1391 2028 2133 2618 1391
Size: 637 105 485 4072
Fragments arranged by size:
4072 637 485 105
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4328
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2104 2138 3490 3524 1903
Size: 34 167 34 1352 34 3678
Fragments arranged by size:
3678 1352 167 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3191 4401 3191
Size: 1210 4089
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5299
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3910 136
Size: 107 122 71 631 605 2238 1525
Fragments arranged by size:
2238 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5294 5294
Size: 5299
BlpI GC'TnA_GC
Cuts at: 2716 2716
Size: 5299
Bme1580I G_kGCm'C
Cuts at: 1790 3360 4516 1790
Size: 1570 1156 2573
Fragments arranged by size:
2573 1570 1156
BmgBI CAC'GTC
Cuts at: 1318 2478 1318
Size: 1160 4139
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2161 3030 3988
Size: 416 53 83 399 24 744 869 958
Cuts at: 3988 442
Size: 1753
Fragments arranged by size:
1753 958 869 744 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5299
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3959 1239
Size: 2720 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2042 3424 4289 4587 2042
Size: 1382 865 298 2754
Fragments arranged by size:
2754 1382 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3962 882
Size: 617 2463 2219
Fragments arranged by size:
2463 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3090 493
Size: 711 1886 2702
Fragments arranged by size:
2702 1886 711
BsaBI GATnn'nnATC
Cuts at: 5293 5293
Size: 5299
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3106 3488 4123 275
Size: 53 83 186 936 1573 382 635 1451
Fragments arranged by size:
1573 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2528 3732 4130 4404 4551 4962 2528
Size: 1204 398 274 147 411 2865
Fragments arranged by size:
2865 1204 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5074
Fragments arranged by size:
5074 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2099 1186
Size: 913 4386
BseYI C'CCAG_C
Cuts at: 4502 4998 4502
Size: 496 4803
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4726
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2749 3510 4538 1540
Size: 275 186 748 761 1028 2301
Fragments arranged by size:
2301 1028 761 748 275 186
BsiHKAI G_wGCw'C
Cuts at: 2133 3360 3445 4516 2133
Size: 1227 85 1071 2916
Fragments arranged by size:
2916 1227 1071 85
BsmI GAATG_Cn'
Cuts at: 2233 2810 2903 2233
Size: 577 93 4629
Fragments arranged by size:
4629 577 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2028 2133 2618 3360 3445 4516 1391
Size: 399 238 105 485 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 485 399 238 105 85
BspEI T'CCGG_A
Cuts at: 4962 4962
Size: 5299
BspHI T'CATG_A
Cuts at: 3188 4918 3188
Size: 1730 3569
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2654 844
Size: 1810 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2470 3186 1394
Size: 429 647 716 3507
Fragments arranged by size:
3507 716 647 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3794 3968 65
Size: 1183 2546 174 1396
Fragments arranged by size:
2546 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2528 3943 4130 1700
Size: 110 718 1415 187 2869
Fragments arranged by size:
2869 1415 718 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5299
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5299
BssSI C'ACGA_G
Cuts at: 3359 4371 3359
Size: 1012 4287
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5299
BstBI TT'CG_AA
Cuts at: 2290 4103 2290
Size: 1813 3486
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5299
BstYI r'GATC_y
Cuts at: 1972 2629 2996 3381 3398 4850 4936 4948 5294
Size: 657 367 385 17 1452 86 12 346
Cuts at: 5294 1972
Size: 1977
Fragments arranged by size:
1977 1452 657 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2070 513
Size: 552 1005 3742
Fragments arranged by size:
3742 1005 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4560
Fragments arranged by size:
4560 683 56
BtsI GCAGTG_nn'
Cuts at: 2815 3609 3629 2815
Size: 794 20 4485
Fragments arranged by size:
4485 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5299
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5264
DraI TTT'AAA
Cuts at: 2603 2603
Size: 5299
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2074 4306 809
Size: 507 758 2232 1802
Fragments arranged by size:
2232 1802 758 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2746 3635 5264
Size: 54 1425 50 275 18 916 889 1629
Cuts at: 5264 8
Size: 43
Fragments arranged by size:
1629 1425 916 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2746 1537
Size: 275 934 4090
Fragments arranged by size:
4090 934 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3229 1353
Size: 1876 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2096 3886 4260 4406 387
Size: 1064 138 507 1790 374 146 1280
Fragments arranged by size:
1790 1280 1064 507 374 146 138
EcoICRI GAG'CTC
Cuts at: 2131 2131
Size: 5299
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3362 3959 4746 5014 1239
Size: 393 1730 597 787 268 1524
Fragments arranged by size:
1730 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2119 2119
Size: 5299
EcoRI G'AATT_C
Cuts at: 2608 2986 2608
Size: 378 4921
FspI TGC'GCA
Cuts at: 3805 3805
Size: 5299
HaeII r_GCGC'y
Cuts at: 1678 4446 1678
Size: 2768 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2075 2107 3940 3972 4014
Size: 32 135 32 503 32 1833 32 42
Cuts at: 4014 4046 1373
Size: 32 2626
Fragments arranged by size:
2626 1833 503 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2643 2889 669
Size: 988 281 705 246 3079
Fragments arranged by size:
3079 988 705 281 246
HindIII A'AGCT_T
Cuts at: 748 2348 748
Size: 1600 3699
HpaI GTT'AAC
Cuts at: 2889 2889
Size: 5299
KpnI G_GTAC'C
Cuts at: 2624 2624
Size: 5299
MfeI C'AATT_G
Cuts at: 2898 2898
Size: 5299
MluI A'CGCG_T
Cuts at: 5175 5175
Size: 5299
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4413 4597 1305
Size: 3108 184 2007
Fragments arranged by size:
3108 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3477
Fragments arranged by size:
3477 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3257 3616 3775 518
Size: 648 2091 359 159 2042
Fragments arranged by size:
2091 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2072 2273 2715 3392 4540 4785 1606
Size: 466 201 442 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 466 442 245 201
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4747
NdeI CA'TA_TG
Cuts at: 387 2333 387
Size: 1946 3353
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5299
NotI GC'GGCC_GC
Cuts at: 2746 2746
Size: 5299
NspI r_CATG'y
Cuts at: 1132 1229 2657 4202 1132
Size: 97 1428 1545 2229
Fragments arranged by size:
2229 1545 1428 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5299
PciI A'CATG_T
Cuts at: 4198 4198
Size: 5299
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5299
PfoI T'CCnGG_A
Cuts at: 2030 2030
Size: 5299
PmeI GTTT'AAAC
Cuts at: 2603 2603
Size: 5299
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3368 3400 4881 4913 3368
Size: 32 1481 32 3754
Fragments arranged by size:
3754 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5299
PsiI TTA'TAA
Cuts at: 2869 2869
Size: 5299
PstI C_TGCA'G
Cuts at: 830 2496 2651 830
Size: 1666 155 3478
Fragments arranged by size:
3478 1666 155
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5299
PvuII CAG'CTG
Cuts at: 2273 2273
Size: 5299
SacI G_AGCT'C
Cuts at: 2133 2133
Size: 5299
SacII CC_GC'GG
Cuts at: 2073 2073
Size: 5299
SalI G'TCGA_C
Cuts at: 1936 2641 1936
Size: 705 4594
SbfI CC_TGCA'GG
Cuts at: 2651 2651
Size: 5299
ScaI AGT'ACT
Cuts at: 3547 5259 3547
Size: 1712 3587
SfcI C'TryA_G
Cuts at: 826 1045 2492 2503 2647 3782 4463 4654 826
Size: 219 1447 11 144 1135 681 191 1471
Fragments arranged by size:
1471 1447 1135 681 219 191 144 11
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5299
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5299
SmaI CCC'GGG
Cuts at: 1872 2626 1872
Size: 754 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2021 3403 4304 4566 820
Size: 197 926 78 1382 901 262 1553
Fragments arranged by size:
1553 1382 926 901 262 197 78
SnaBI TAC'GTA
Cuts at: 493 3090 493
Size: 2597 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5299
SphI G_CATG'C
Cuts at: 1132 2657 1132
Size: 1525 3774
SspI AAT'ATT
Cuts at: 5 52 3223 5
Size: 47 3171 2081
Fragments arranged by size:
3171 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5299
StyI C'CwwG_G
Cuts at: 513 1065 2738 513
Size: 552 1673 3074
Fragments arranged by size:
3074 1673 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2123 3326 3343 3496 3681 4126 325
Size: 1255 543 1203 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1203 543 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3545 5257 96
Size: 274 80 33 51 1443 1568 1712 138
Fragments arranged by size:
1712 1568 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5259 5291 5259
Size: 32 5267
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5299
XbaI T'CTAG_A
Cuts at: 2635 2635
Size: 5299
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5299
XmaI C'CCGG_G
Cuts at: 1870 2624 1870
Size: 754 4545
XmnI GAAnn'nnTTC
Cuts at: 3428 3428
Size: 5299
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3107 276
Size: 53 83 186 936 1573 2468
Fragments arranged by size:
2468 1573 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI RsrII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI