Restriction map for
pFN21AE0628
(Circular) (Six-base) MAPSORT of: pFN21AE0628.seq Check: 6303 from: 1 to: 5821
pFN21AE0628 5821 bp
With 182 enzymes: SgfI *
January 30, 2014 17:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2336 2336
Size: 5821
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3631 278
Size: 53 83 186 936 2095 2468
Fragments arranged by size:
2468 2095 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3164 1937
Size: 1227 4594
Acc65I G'GTAC_C
Cuts at: 3142 3142
Size: 5821
AclI AA'CG_TT
Cuts at: 1563 3948 4321 1563
Size: 2385 373 3063
Fragments arranged by size:
3063 2385 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2937 2988 3884 5268 5536 1632
Size: 1305 51 896 1384 268 1917
Fragments arranged by size:
1917 1384 1305 896 268 51
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5624
AflIII A'CryG_T
Cuts at: 1203 4720 5697 1203
Size: 3517 977 1327
Fragments arranged by size:
3517 1327 977
AgeI A'CCGG_T
Cuts at: 4652 4652
Size: 5821
AhdI GACnn_n'nnGTC
Cuts at: 4550 4550
Size: 5821
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5821
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2878 1184
Size: 1694 4127
AlwNI CAG_nnn'CTG
Cuts at: 3564 5136 3564
Size: 1572 4249
ApaLI G'TGCA_C
Cuts at: 3878 5034 3878
Size: 1156 4665
ApoI r'AATT_y
Cuts at: 1493 2064 2132 2189 2232 2355 3130 3356 3508
Size: 571 68 57 43 123 775 226 152
Cuts at: 3508 5617 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 775 571 226 152 123 68
57 43
AseI AT'TA_AT
Cuts at: 160 1032 2286 4375 160
Size: 872 1254 2089 1606
Fragments arranged by size:
2089 1606 1254 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5821
AvaI C'yCGr_G
Cuts at: 1870 1943 2519 3146 1870
Size: 73 576 627 4545
Fragments arranged by size:
4545 627 576 73
BamHI G'GATC_C
Cuts at: 3151 3518 3151
Size: 367 5454
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3142 4597 618
Size: 325 217 627 1355 1455 1842
Fragments arranged by size:
1842 1455 1355 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3140 1391
Size: 1749 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2976 3024 928
Size: 971 1077 48 3725
Fragments arranged by size:
3725 1077 971 48
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4012 4046 1903
Size: 34 2075 34 3678
Fragments arranged by size:
3678 2075 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3713 4923 3713
Size: 1210 4611
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5821
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4432 136
Size: 107 122 71 631 605 2760 1525
Fragments arranged by size:
2760 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5816 5816
Size: 5821
BlpI GC'TnA_GC
Cuts at: 3238 3238
Size: 5821
Bme1580I G_kGCm'C
Cuts at: 1790 3882 5038 1790
Size: 2092 1156 2573
Fragments arranged by size:
2573 2092 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5821
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3552 4510 442
Size: 416 53 83 399 24 2135 958 1753
Fragments arranged by size:
2135 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5821
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2598 4481 1239
Size: 1359 1883 2579
Fragments arranged by size:
2579 1883 1359
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2733 3946 4811 5109 2733
Size: 1213 865 298 3445
Fragments arranged by size:
3445 1213 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4484 882
Size: 617 2985 2219
Fragments arranged by size:
2985 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3612 493
Size: 711 2408 2702
Fragments arranged by size:
2702 2408 711
BsaBI GATnn'nnATC
Cuts at: 5815 5815
Size: 5821
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3628 4010 4645 275
Size: 53 83 186 936 2095 382 635 1451
Fragments arranged by size:
2095 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4254 4652 4926 5073 5484 4254
Size: 398 274 147 411 4591
Fragments arranged by size:
4591 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2041 2071 1178
Size: 30 165 30 638 30 4928
Fragments arranged by size:
4928 638 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2026 2029 2049 1186
Size: 840 3 20 4958
Fragments arranged by size:
4958 840 20 3
BseYI C'CCAG_C
Cuts at: 5024 5520 5024
Size: 496 5325
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5248
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3271 4032 5060 1540
Size: 275 186 1270 761 1028 2301
Fragments arranged by size:
2301 1270 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3882 3967 5038 3882
Size: 85 1071 4665
Fragments arranged by size:
4665 1071 85
BsmI GAATG_Cn'
Cuts at: 3332 3425 3332
Size: 93 5728
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3140 3882 3967 5038 1391
Size: 399 1350 742 85 1071 2174
Fragments arranged by size:
2174 1350 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5484 5484
Size: 5821
BspHI T'CATG_A
Cuts at: 2293 3710 5440 2293
Size: 1417 1730 2674
Fragments arranged by size:
2674 1730 1417
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2336 3176 844
Size: 1492 840 3489
Fragments arranged by size:
3489 1492 840
BsrBI CCG'CTC
Cuts at: 1394 1823 3708 1394
Size: 429 1885 3507
Fragments arranged by size:
3507 1885 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4316 4490 65
Size: 1183 3068 174 1396
Fragments arranged by size:
3068 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4465 4652 1700
Size: 110 2655 187 2869
Fragments arranged by size:
2869 2655 187 110
BsrGI T'GTAC_A
Cuts at: 96 2467 96
Size: 2371 3450
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5821
BssSI C'ACGA_G
Cuts at: 2240 3881 4893 2240
Size: 1641 1012 3168
Fragments arranged by size:
3168 1641 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5821
BstBI TT'CG_AA
Cuts at: 4625 4625
Size: 5821
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5821
BstYI r'GATC_y
Cuts at: 1972 3151 3518 3903 3920 5372 5458 5470 5816
Size: 1179 367 385 17 1452 86 12 346
Cuts at: 5816 1972
Size: 1977
Fragments arranged by size:
1977 1452 1179 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5269
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5082
Fragments arranged by size:
5082 683 56
BtsI GCAGTG_nn'
Cuts at: 2894 3337 4131 4151 2894
Size: 443 794 20 4564
Fragments arranged by size:
4564 794 443 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5821
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5786
DraI TTT'AAA
Cuts at: 2481 3125 2481
Size: 644 5177
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2220 4828 809
Size: 507 904 2608 1802
Fragments arranged by size:
2608 1802 904 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2170 2799 3268
Size: 54 1425 50 275 18 340 629 469
Cuts at: 3268 4157 5786 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 629 469 340 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3268 1537
Size: 275 1456 4090
Fragments arranged by size:
4090 1456 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2203 3751 1353
Size: 850 1548 3423
Fragments arranged by size:
3423 1548 850
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4408 4782 4928 387
Size: 1064 138 2819 374 146 1280
Fragments arranged by size:
2819 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2598 2937 2988 3884 4481 5268 5536
Size: 393 966 339 51 896 597 787 268
Cuts at: 5536 1239
Size: 1524
Fragments arranged by size:
1524 966 896 787 597 393 339 268
51
EcoO109I rG'GnC_Cy
Cuts at: 2961 2961
Size: 5821
EcoRI G'AATT_C
Cuts at: 3130 3508 3130
Size: 378 5443
FspI TGC'GCA
Cuts at: 4327 4327
Size: 5821
HaeII r_GCGC'y
Cuts at: 1678 4968 1678
Size: 3290 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4462 4494 4536 4568 1373
Size: 32 135 32 2890 32 42 32 2626
Fragments arranged by size:
2890 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2691 3165 3411 669
Size: 988 281 753 474 246 3079
Fragments arranged by size:
3079 988 753 474 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5821
HpaI GTT'AAC
Cuts at: 2691 3411 2691
Size: 720 5101
KpnI G_GTAC'C
Cuts at: 3146 3146
Size: 5821
MfeI C'AATT_G
Cuts at: 3420 3420
Size: 5821
MluI A'CGCG_T
Cuts at: 5697 5697
Size: 5821
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4935 5119 1305
Size: 3630 184 2007
Fragments arranged by size:
3630 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2172 2801 10
Size: 54 1425 343 340 629 3030
Fragments arranged by size:
3030 1425 629 343 340 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2571 3779 4138 4297 518
Size: 648 1405 1208 359 159 2042
Fragments arranged by size:
2042 1405 1208 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2797 3237 3914 5062 5307 1606
Size: 1191 440 677 1148 245 2120
Fragments arranged by size:
2120 1191 1148 677 440 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5269
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5821
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5821
NotI GC'GGCC_GC
Cuts at: 3268 3268
Size: 5821
NspI r_CATG'y
Cuts at: 1132 1229 2570 3179 4724 1132
Size: 97 1341 609 1545 2229
Fragments arranged by size:
2229 1545 1341 609 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5821
PciI A'CATG_T
Cuts at: 4720 4720
Size: 5821
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5821
PmeI GTTT'AAAC
Cuts at: 3125 3125
Size: 5821
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3890 3922 5403 5435 3890
Size: 32 1481 32 4276
Fragments arranged by size:
4276 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2961 2961
Size: 5821
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5821
PsiI TTA'TAA
Cuts at: 3391 3391
Size: 5821
PstI C_TGCA'G
Cuts at: 830 2347 3173 830
Size: 1517 826 3478
Fragments arranged by size:
3478 1517 826
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5821
PvuII CAG'CTG
Cuts at: 2797 2797
Size: 5821
SalI G'TCGA_C
Cuts at: 1936 3163 1936
Size: 1227 4594
SbfI CC_TGCA'GG
Cuts at: 3173 3173
Size: 5821
ScaI AGT'ACT
Cuts at: 4069 5781 4069
Size: 1712 4109
SfcI C'TryA_G
Cuts at: 826 1045 2343 2882 3169 4304 4985 5176 826
Size: 219 1298 539 287 1135 681 191 1471
Fragments arranged by size:
1471 1298 1135 681 539 287 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5821
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5821
SmaI CCC'GGG
Cuts at: 1872 3148 1872
Size: 1276 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2748 3925 4826 5088 820
Size: 197 926 805 1177 901 262 1553
Fragments arranged by size:
1553 1177 926 901 805 262 197
SnaBI TAC'GTA
Cuts at: 493 3612 493
Size: 3119 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5821
SphI G_CATG'C
Cuts at: 1132 2570 3179 1132
Size: 1438 609 3774
Fragments arranged by size:
3774 1438 609
SspI AAT'ATT
Cuts at: 5 52 2606 2665 3745 5
Size: 47 2554 59 1080 2081
Fragments arranged by size:
2554 2081 1080 59 47
StuI AGG'CCT
Cuts at: 1517 2043 1517
Size: 526 5295
StyI C'CwwG_G
Cuts at: 513 1065 3260 513
Size: 552 2195 3074
Fragments arranged by size:
3074 2195 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3848 3865 4018 4203 4648 325
Size: 1255 2268 17 153 185 445 1498
Fragments arranged by size:
2268 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2467 4067 5779
Size: 274 80 33 51 1443 490 1600 1712
Cuts at: 5779 96
Size: 138
Fragments arranged by size:
1712 1600 1443 490 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5781 5813 5781
Size: 32 5789
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5821
XbaI T'CTAG_A
Cuts at: 3157 3157
Size: 5821
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5821
XmaI C'CCGG_G
Cuts at: 1870 3146 1870
Size: 1276 4545
XmnI GAAnn'nnTTC
Cuts at: 2035 3950 2035
Size: 1915 3906
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3629 276
Size: 53 83 186 936 2095 2468
Fragments arranged by size:
2468 2095 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI