Restriction map for
pFN21AE0620
(Circular) (Six-base) MAPSORT of: pFN21AE0620.seq Check: 951 from: 1 to: 5785
pFN21AE0620 5785 bp
With 182 enzymes: SgfI *
October 20, 2009 10:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3595 278
Size: 53 83 186 936 2059 2468
Fragments arranged by size:
2468 2059 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3128 1937
Size: 1191 4594
Acc65I G'GTAC_C
Cuts at: 3106 3106
Size: 5785
AclI AA'CG_TT
Cuts at: 1563 3912 4285 1563
Size: 2349 373 3063
Fragments arranged by size:
3063 2349 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3848 5232 5500 1632
Size: 2216 1384 268 1917
Fragments arranged by size:
2216 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5588
AflIII A'CryG_T
Cuts at: 1203 4684 5661 1203
Size: 3481 977 1327
Fragments arranged by size:
3481 1327 977
AgeI A'CCGG_T
Cuts at: 4616 4616
Size: 5785
AhdI GACnn_n'nnGTC
Cuts at: 2200 4514 2200
Size: 2314 3471
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5785
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5785
AlwNI CAG_nnn'CTG
Cuts at: 2020 2644 3528 5100 2020
Size: 624 884 1572 2705
Fragments arranged by size:
2705 1572 884 624
ApaI G_GGCC'C
Cuts at: 2054 2054
Size: 5785
ApaLI G'TGCA_C
Cuts at: 3842 4998 3842
Size: 1156 4629
ApoI r'AATT_y
Cuts at: 1493 3094 3320 3472 5581 1493
Size: 1601 226 152 2109 1697
Fragments arranged by size:
2109 1697 1601 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4339 160
Size: 872 3307 1606
Fragments arranged by size:
3307 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5785
AvaI C'yCGr_G
Cuts at: 1870 1943 2499 3110 1870
Size: 73 556 611 4545
Fragments arranged by size:
4545 611 556 73
BamHI G'GATC_C
Cuts at: 3115 3482 3115
Size: 367 5418
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2825 3106 4561 618
Size: 325 217 627 1038 281 1455 1842
Fragments arranged by size:
1842 1455 1038 627 325 281 217
BanII G_rGCy'C
Cuts at: 1391 2054 2400 2595 3104 1391
Size: 663 346 195 509 4072
Fragments arranged by size:
4072 663 509 346 195
BbeI G_GCGC'C
Cuts at: 2829 2829
Size: 5785
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2333 2636 928
Size: 971 434 303 4077
Fragments arranged by size:
4077 971 434 303
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2944 2978 3976 4010 1903
Size: 34 1007 34 998 34 3678
Fragments arranged by size:
3678 1007 998 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3677 4887 3677
Size: 1210 4575
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5785
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2058 2063 2077
Size: 107 122 71 631 605 386 5 14
Cuts at: 2077 2546 2796 3021 4396 136
Size: 469 250 225 1375 1525
Fragments arranged by size:
1525 1375 631 605 469 386 250 225
122 107 71 14 5
BglII A'GATC_T
Cuts at: 5780 5780
Size: 5785
BlpI GC'TnA_GC
Cuts at: 2589 3202 2589
Size: 613 5172
Bme1580I G_kGCm'C
Cuts at: 1790 2054 3846 5002 1790
Size: 264 1792 1156 2573
Fragments arranged by size:
2573 1792 1156 264
BmgBI CAC'GTC
Cuts at: 1318 2463 1318
Size: 1145 4640
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2030 2349 2437
Size: 416 53 83 399 24 613 319 88
Cuts at: 2437 3516 4474 442
Size: 1079 958 1753
Fragments arranged by size:
1753 1079 958 613 416 399 319 88
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5785
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4445 1239
Size: 3206 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3910 4775 5073 3910
Size: 865 298 4622
Fragments arranged by size:
4622 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3058 4448 882
Size: 617 1559 1390 2219
Fragments arranged by size:
2219 1559 1390 617
BsaAI yAC'GTr
Cuts at: 493 1204 3576 493
Size: 711 2372 2702
Fragments arranged by size:
2702 2372 711
BsaBI GATnn'nnATC
Cuts at: 2771 5779 2771
Size: 3008 2777
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2748 2826 3592 3974
Size: 53 83 186 936 1215 78 766 382
Cuts at: 3974 4609 275
Size: 635 1451
Fragments arranged by size:
1451 1215 936 766 635 382 186 83
78 53
BsaWI w'CCGG_w
Cuts at: 2860 4218 4616 4890 5037 5448 2860
Size: 1358 398 274 147 411 3197
Fragments arranged by size:
3197 1358 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5560
Fragments arranged by size:
5560 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5785
BseYI C'CCAG_C
Cuts at: 2578 2706 4988 5484 2578
Size: 128 2282 496 2879
Fragments arranged by size:
2879 2282 496 128
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5212
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2058 2452 2769 3235 3996 5024
Size: 275 186 57 394 317 466 761 1028
Cuts at: 5024 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 466 394 317 275 186
57
BsiHKAI G_wGCw'C
Cuts at: 3846 3931 5002 3846
Size: 85 1071 4629
Fragments arranged by size:
4629 1071 85
BsmI GAATG_Cn'
Cuts at: 3296 3389 3296
Size: 93 5692
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2054 2400 2595 3104 3846 3931 5002
Size: 399 264 346 195 509 742 85 1071
Cuts at: 5002 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 509 399 346 264 195
85
BspEI T'CCGG_A
Cuts at: 5448 5448
Size: 5785
BspHI T'CATG_A
Cuts at: 3674 5404 3674
Size: 1730 4055
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2148 3040 3140 844
Size: 1304 892 100 3489
Fragments arranged by size:
3489 1304 892 100
BsrBI CCG'CTC
Cuts at: 1394 1823 2787 3672 1394
Size: 429 964 885 3507
Fragments arranged by size:
3507 964 885 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4280 4454 65
Size: 1183 3032 174 1396
Fragments arranged by size:
3032 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2057 2828 4429 4616 1700
Size: 110 247 771 1601 187 2869
Fragments arranged by size:
2869 1601 771 247 187 110
BsrGI T'GTAC_A
Cuts at: 96 2847 96
Size: 2751 3034
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5785
BssSI C'ACGA_G
Cuts at: 3845 4857 3845
Size: 1012 4773
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2644 794
Size: 1850 3935
BstBI TT'CG_AA
Cuts at: 4589 4589
Size: 5785
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5785
BstYI r'GATC_y
Cuts at: 1972 3115 3482 3867 3884 5336 5422 5434 5780
Size: 1143 367 385 17 1452 86 12 346
Cuts at: 5780 1972
Size: 1977
Fragments arranged by size:
1977 1452 1143 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2028 3000 513
Size: 552 939 24 972 3298
Fragments arranged by size:
3298 972 939 552 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2992 487
Size: 683 56 1766 3280
Fragments arranged by size:
3280 1766 683 56
BtsI GCAGTG_nn'
Cuts at: 3301 4095 4115 3301
Size: 794 20 4971
Fragments arranged by size:
4971 794 20
ClaI AT'CG_AT
Cuts at: 1554 2775 1554
Size: 1221 4564
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5750
DraI TTT'AAA
Cuts at: 3089 3089
Size: 5785
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4792 809
Size: 507 3476 1802
Fragments arranged by size:
3476 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2055 2449 2997
Size: 54 1425 50 275 18 225 394 548
Cuts at: 2997 3003 3232 4121 5750 8
Size: 6 229 889 1629 43
Fragments arranged by size:
1629 1425 889 548 394 275 229 225
54 50 43 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 2055 2449 3232 1537
Size: 275 243 394 783 4090
Fragments arranged by size:
4090 783 394 275 243
EarI CTCTTCn'nnn_
Cuts at: 1353 2183 2441 2538 3715 1353
Size: 830 258 97 1177 3423
Fragments arranged by size:
3423 1177 830 258 97
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2659 4372 4746 4892 387
Size: 1064 138 1070 1713 374 146 1280
Fragments arranged by size:
1713 1280 1070 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3848 4445 5232 5500 1239
Size: 393 2216 597 787 268 1524
Fragments arranged by size:
2216 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2050 2687 2050
Size: 637 5148
EcoRI G'AATT_C
Cuts at: 3094 3472 3094
Size: 378 5407
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2194 2194
Size: 5785
FseI GG_CCGG'CC
Cuts at: 2061 2061
Size: 5785
FspI TGC'GCA
Cuts at: 4291 4291
Size: 5785
HaeII r_GCGC'y
Cuts at: 1678 2645 2680 2829 4932 1678
Size: 967 35 149 2103 2531
Fragments arranged by size:
2531 2103 967 149 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2186 2218 4426 4458 4500
Size: 32 135 32 614 32 2208 32 42
Cuts at: 4500 4532 1373
Size: 32 2626
Fragments arranged by size:
2626 2208 614 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2134 3129 3375 669
Size: 988 281 196 995 246 3079
Fragments arranged by size:
3079 995 988 281 246 196
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5785
HpaI GTT'AAC
Cuts at: 3375 3375
Size: 5785
KasI G'GCGC_C
Cuts at: 2825 2825
Size: 5785
KpnI G_GTAC'C
Cuts at: 3110 3110
Size: 5785
MfeI C'AATT_G
Cuts at: 3384 3384
Size: 5785
MluI A'CGCG_T
Cuts at: 5661 5661
Size: 5785
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4899 5083 1305
Size: 3594 184 2007
Fragments arranged by size:
3594 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3005 10
Size: 54 1425 343 1173 2790
Fragments arranged by size:
2790 1425 1173 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2336 2474 2843 3743 4102 4261 518
Size: 648 1170 138 369 900 359 159 2042
Fragments arranged by size:
2042 1170 900 648 369 359 159 138
MspA1I CmG'CkG
Cuts at: 1606 2394 2578 2588 2890 3201 3878 5026 5271
Size: 788 184 10 302 311 677 1148 245
Cuts at: 5271 1606
Size: 2120
Fragments arranged by size:
2120 1148 788 677 311 302 245 184
10
NaeI GCC'GGC
Cuts at: 2059 2830 2059
Size: 771 5014
NarI GG'CG_CC
Cuts at: 2826 2826
Size: 5785
NcoI C'CATG_G
Cuts at: 513 1065 2004 2028 3000 513
Size: 552 939 24 972 3298
Fragments arranged by size:
3298 972 939 552 24
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5785
NgoMIV G'CCGG_C
Cuts at: 2057 2828 2057
Size: 771 5014
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5785
NotI GC'GGCC_GC
Cuts at: 3232 3232
Size: 5785
NruI TCG'CGA
Cuts at: 2612 2612
Size: 5785
NspI r_CATG'y
Cuts at: 1132 1229 3143 4688 1132
Size: 97 1914 1545 2229
Fragments arranged by size:
2229 1914 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5785
PciI A'CATG_T
Cuts at: 4684 4684
Size: 5785
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2087 1108
Size: 979 4806
PfoI T'CCnGG_A
Cuts at: 2370 2370
Size: 5785
PmeI GTTT'AAAC
Cuts at: 3089 3089
Size: 5785
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3854 3886 5367 5399 3854
Size: 32 1481 32 4240
Fragments arranged by size:
4240 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5785
PsiI TTA'TAA
Cuts at: 3355 3355
Size: 5785
PspOMI G'GGCC_C
Cuts at: 2050 2050
Size: 5785
PstI C_TGCA'G
Cuts at: 830 3051 3137 830
Size: 2221 86 3478
Fragments arranged by size:
3478 2221 86
PvuI CG_AT'CG
Cuts at: 2001 2769 2001
Size: 768 5017
PvuII CAG'CTG
Cuts at: 2588 2890 2588
Size: 302 5483
SalI G'TCGA_C
Cuts at: 1936 3127 1936
Size: 1191 4594
SapI GCTCTTCn'nnn_
Cuts at: 2441 2441
Size: 5785
SbfI CC_TGCA'GG
Cuts at: 3137 3137
Size: 5785
ScaI AGT'ACT
Cuts at: 4033 5745 4033
Size: 1712 4073
SexAI A'CCwGG_T
Cuts at: 2481 2481
Size: 5785
SfcI C'TryA_G
Cuts at: 826 1045 3047 3133 4268 4949 5140 826
Size: 219 2002 86 1135 681 191 1471
Fragments arranged by size:
2002 1471 1135 681 219 191 86
SfiI GGCCn_nnn'nGGCC
Cuts at: 2058 2063 2058
Size: 5 5780
SfoI GGC'GCC
Cuts at: 2827 2827
Size: 5785
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5785
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5785
SmaI CCC'GGG
Cuts at: 1872 3112 1872
Size: 1240 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3889 4790 5052 820
Size: 197 926 1946 901 262 1553
Fragments arranged by size:
1946 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3576 493
Size: 3083 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5785
SphI G_CATG'C
Cuts at: 1132 3143 1132
Size: 2011 3774
SspI AAT'ATT
Cuts at: 5 52 3709 5
Size: 47 3657 2081
Fragments arranged by size:
3657 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5785
StyI C'CwwG_G
Cuts at: 513 1065 2004 2028 2086 2242 3000 3224 513
Size: 552 939 24 58 156 758 224 3074
Fragments arranged by size:
3074 939 758 552 224 156 58 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2666 3812 3829 3982 4167 4612 325
Size: 1255 1086 1146 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1146 1086 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2847 4031 5743
Size: 274 80 33 51 1443 870 1184 1712
Cuts at: 5743 96
Size: 138
Fragments arranged by size:
1712 1443 1184 870 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5745 5777 5745
Size: 32 5753
Tth111I GACn'n_nGTC
Cuts at: 1316 2483 1316
Size: 1167 4618
XbaI T'CTAG_A
Cuts at: 3121 3121
Size: 5785
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5785
XmaI C'CCGG_G
Cuts at: 1870 3110 1870
Size: 1240 4545
XmnI GAAnn'nnTTC
Cuts at: 3914 3914
Size: 5785
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3593 276
Size: 53 83 186 936 2059 2468
Fragments arranged by size:
2468 2059 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FspAI NsiI PacI PmlI PpuMI
PspXI PsrI RsrII SacI SacII SanDI SrfI SwaI
XcmI