Restriction map for
pFN21AE0614
(Circular) (Six-base) MAPSORT of: pFN21AE0614.seq Check: 193 from: 1 to: 5797
pFN21AE0614 5797 bp
With 182 enzymes: SgfI *
October 20, 2009 10:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3607 278
Size: 53 83 186 936 2071 2468
Fragments arranged by size:
2468 2071 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3140 1937
Size: 1203 4594
Acc65I G'GTAC_C
Cuts at: 3118 3118
Size: 5797
AclI AA'CG_TT
Cuts at: 1563 3924 4297 1563
Size: 2361 373 3063
Fragments arranged by size:
3063 2361 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2795 3860 5244 5512 1632
Size: 1163 1065 1384 268 1917
Fragments arranged by size:
1917 1384 1163 1065 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5600
AflIII A'CryG_T
Cuts at: 1203 2847 4696 5673 1203
Size: 1644 1849 977 1327
Fragments arranged by size:
1849 1644 1327 977
AgeI A'CCGG_T
Cuts at: 4628 4628
Size: 5797
AhdI GACnn_n'nnGTC
Cuts at: 4526 4526
Size: 5797
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5797
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5797
AlwNI CAG_nnn'CTG
Cuts at: 3540 5112 3540
Size: 1572 4225
ApaLI G'TGCA_C
Cuts at: 3854 5010 3854
Size: 1156 4641
ApoI r'AATT_y
Cuts at: 1493 2885 2906 3106 3332 3484 5593 1493
Size: 1392 21 200 226 152 2109 1697
Fragments arranged by size:
2109 1697 1392 226 200 152 21
AseI AT'TA_AT
Cuts at: 160 1032 4351 160
Size: 872 3319 1606
Fragments arranged by size:
3319 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5797
AvaI C'yCGr_G
Cuts at: 1870 1943 3122 1870
Size: 73 1179 4545
Fragments arranged by size:
4545 1179 73
BamHI G'GATC_C
Cuts at: 3127 3494 3127
Size: 367 5430
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2599 3118 4573 618
Size: 325 217 627 812 519 1455 1842
Fragments arranged by size:
1842 1455 812 627 519 325 217
BanII G_rGCy'C
Cuts at: 1391 3116 1391
Size: 1725 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2620 2762 2783 928
Size: 971 721 142 21 3942
Fragments arranged by size:
3942 971 721 142 21
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2075 2109 3988 4022 1903
Size: 34 138 34 1879 34 3678
Fragments arranged by size:
3678 1879 138 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2749 3689 4899 2749
Size: 940 1210 3647
Fragments arranged by size:
3647 1210 940
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5797
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4408 136
Size: 107 122 71 631 605 2736 1525
Fragments arranged by size:
2736 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5792 5792
Size: 5797
BlpI GC'TnA_GC
Cuts at: 3214 3214
Size: 5797
Bme1580I G_kGCm'C
Cuts at: 1790 3858 5014 1790
Size: 2068 1156 2573
Fragments arranged by size:
2573 2068 1156
BmgBI CAC'GTC
Cuts at: 1318 2848 2869 1318
Size: 1530 21 4246
Fragments arranged by size:
4246 1530 21
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2041 2544 3528
Size: 416 53 83 399 24 624 503 984
Cuts at: 3528 4486 442
Size: 958 1753
Fragments arranged by size:
1753 984 958 624 503 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5797
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2739 2739
Size: 5797
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3087 4457 1239
Size: 1848 1370 2579
Fragments arranged by size:
2579 1848 1370
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3922 4787 5085 3922
Size: 865 298 4634
Fragments arranged by size:
4634 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4460 882
Size: 617 2961 2219
Fragments arranged by size:
2961 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3588 493
Size: 711 2384 2702
Fragments arranged by size:
2702 2384 711
BsaBI GATnn'nnATC
Cuts at: 5791 5791
Size: 5797
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3604 3986 4621 275
Size: 53 83 186 936 2071 382 635 1451
Fragments arranged by size:
2071 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4230 4628 4902 5049 5460 4230
Size: 398 274 147 411 4567
Fragments arranged by size:
4567 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5572
Fragments arranged by size:
5572 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5797
BseYI C'CCAG_C
Cuts at: 2297 5000 5496 2297
Size: 2703 496 2598
Fragments arranged by size:
2703 2598 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5224
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3247 4008 5036 1540
Size: 275 186 1246 761 1028 2301
Fragments arranged by size:
2301 1246 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3858 3943 5014 3858
Size: 85 1071 4641
Fragments arranged by size:
4641 1071 85
BsmI GAATG_Cn'
Cuts at: 3308 3401 3308
Size: 93 5704
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3116 3858 3943 5014 1391
Size: 399 1326 742 85 1071 2174
Fragments arranged by size:
2174 1326 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5460 5460
Size: 5797
BspHI T'CATG_A
Cuts at: 3686 5416 3686
Size: 1730 4067
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3152 844
Size: 2308 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3033 3684 1394
Size: 429 1210 651 3507
Fragments arranged by size:
3507 1210 651 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2091 3085 4292 4466 65
Size: 1183 843 994 1207 174 1396
Fragments arranged by size:
1396 1207 1183 994 843 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2487 3008 4441 4628 1700
Size: 110 677 521 1433 187 2869
Fragments arranged by size:
2869 1433 677 521 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5797
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5797
BssSI C'ACGA_G
Cuts at: 3857 4869 3857
Size: 1012 4785
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5797
BstBI TT'CG_AA
Cuts at: 4601 4601
Size: 5797
BstEII G'GTnAC_C
Cuts at: 1184 2650 1184
Size: 1466 4331
BstXI CCAn_nnnn'nTGG
Cuts at: 2209 2422 2536 2209
Size: 213 114 5470
Fragments arranged by size:
5470 213 114
BstYI r'GATC_y
Cuts at: 1972 2976 3127 3494 3879 3896 5348 5434 5446
Size: 1004 151 367 385 17 1452 86 12
Cuts at: 5446 5792 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1004 385 367 346 151 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4306
Fragments arranged by size:
4306 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5058
Fragments arranged by size:
5058 683 56
BtsI GCAGTG_nn'
Cuts at: 3001 3313 4107 4127 3001
Size: 312 794 20 4671
Fragments arranged by size:
4671 794 312 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5797
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5762
DraI TTT'AAA
Cuts at: 3101 3101
Size: 5797
DraIII CAC_nnn'GTG
Cuts at: 3091 3091
Size: 5797
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4804 809
Size: 507 3488 1802
Fragments arranged by size:
3488 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2199 2412 2526
Size: 54 1425 50 275 18 369 213 114
Cuts at: 2526 3244 4133 5762 8
Size: 718 889 1629 43
Fragments arranged by size:
1629 1425 889 718 369 275 213 114
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3244 1537
Size: 275 1432 4090
Fragments arranged by size:
4090 1432 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3727 1353
Size: 2374 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2761 4384 4758 4904 387
Size: 1064 138 1172 1623 374 146 1280
Fragments arranged by size:
1623 1280 1172 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2795 3087 3860 4457 5244 5512 1239
Size: 393 1163 292 773 597 787 268 1524
Fragments arranged by size:
1524 1163 787 773 597 393 292 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2375 2375
Size: 5797
EcoO109I rG'GnC_Cy
Cuts at: 2333 2380 2799 2333
Size: 47 419 5331
Fragments arranged by size:
5331 419 47
EcoRI G'AATT_C
Cuts at: 2906 3106 3484 2906
Size: 200 378 5219
Fragments arranged by size:
5219 378 200
FspI TGC'GCA
Cuts at: 4303 4303
Size: 5797
HaeII r_GCGC'y
Cuts at: 1678 2964 4944 1678
Size: 1286 1980 2531
Fragments arranged by size:
2531 1980 1286
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2157 2189 4438 4470 4512
Size: 32 135 32 585 32 2249 32 42
Cuts at: 4512 4544 1373
Size: 32 2626
Fragments arranged by size:
2626 2249 585 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2233 3141 3387 669
Size: 988 281 295 908 246 3079
Fragments arranged by size:
3079 988 908 295 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5797
HpaI GTT'AAC
Cuts at: 3387 3387
Size: 5797
KpnI G_GTAC'C
Cuts at: 3122 3122
Size: 5797
MfeI C'AATT_G
Cuts at: 3396 3396
Size: 5797
MluI A'CGCG_T
Cuts at: 5673 5673
Size: 5797
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2616 4911 5095 1305
Size: 1311 2295 184 2007
Fragments arranged by size:
2295 2007 1311 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2201 2414 2528 10
Size: 54 1425 343 369 213 114 3279
Fragments arranged by size:
3279 1425 369 343 213 114 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2057 2477 2534 3078 3755 4114 4273
Size: 648 891 420 57 544 677 359 159
Cuts at: 4273 518
Size: 2042
Fragments arranged by size:
2042 891 677 648 544 420 359 159
57
MspA1I CmG'CkG
Cuts at: 1606 3213 3890 5038 5283 1606
Size: 1607 677 1148 245 2120
Fragments arranged by size:
2120 1607 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4306
Fragments arranged by size:
4306 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5797
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5797
NotI GC'GGCC_GC
Cuts at: 3244 3244
Size: 5797
NspI r_CATG'y
Cuts at: 1132 1229 2464 3155 4700 1132
Size: 97 1235 691 1545 2229
Fragments arranged by size:
2229 1545 1235 691 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5797
PciI A'CATG_T
Cuts at: 4696 4696
Size: 5797
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2136 3091 1108
Size: 1028 955 3814
Fragments arranged by size:
3814 1028 955
PfoI T'CCnGG_A
Cuts at: 2821 2821
Size: 5797
PmeI GTTT'AAAC
Cuts at: 3101 3101
Size: 5797
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3866 3898 5379 5411 3866
Size: 32 1481 32 4252
Fragments arranged by size:
4252 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2333 2333
Size: 5797
PshAI GACnn'nnGTC
Cuts at: 1613 2783 1613
Size: 1170 4627
PsiI TTA'TAA
Cuts at: 2106 3367 2106
Size: 1261 4536
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2655 2687 2655
Size: 32 5765
PstI C_TGCA'G
Cuts at: 830 2287 3149 830
Size: 1457 862 3478
Fragments arranged by size:
3478 1457 862
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5797
SalI G'TCGA_C
Cuts at: 1936 3139 1936
Size: 1203 4594
SbfI CC_TGCA'GG
Cuts at: 3149 3149
Size: 5797
ScaI AGT'ACT
Cuts at: 4045 5757 4045
Size: 1712 4085
SfcI C'TryA_G
Cuts at: 826 1045 2283 2608 3145 4280 4961 5152 826
Size: 219 1238 325 537 1135 681 191 1471
Fragments arranged by size:
1471 1238 1135 681 537 325 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5797
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5797
SmaI CCC'GGG
Cuts at: 1872 3124 1872
Size: 1252 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3901 4802 5064 820
Size: 197 926 1958 901 262 1553
Fragments arranged by size:
1958 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3588 493
Size: 3095 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5797
SphI G_CATG'C
Cuts at: 1132 2464 3155 1132
Size: 1332 691 3774
Fragments arranged by size:
3774 1332 691
SspI AAT'ATT
Cuts at: 5 52 3721 5
Size: 47 3669 2081
Fragments arranged by size:
3669 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5797
StyI C'CwwG_G
Cuts at: 513 1065 2004 3236 513
Size: 552 939 1232 3074
Fragments arranged by size:
3074 1232 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2528 3824 3841 3994 4179 4624 325
Size: 1255 948 1296 17 153 185 445 1498
Fragments arranged by size:
1498 1296 1255 948 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4043 5755 96
Size: 274 80 33 51 1443 2066 1712 138
Fragments arranged by size:
2066 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5757 5789 5757
Size: 32 5765
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5797
XbaI T'CTAG_A
Cuts at: 2842 3133 2842
Size: 291 5506
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2644 2644
Size: 5797
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5797
XmaI C'CCGG_G
Cuts at: 1870 3122 1870
Size: 1252 4545
XmnI GAAnn'nnTTC
Cuts at: 2888 3926 2888
Size: 1038 4759
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3605 276
Size: 53 83 186 936 2071 2468
Fragments arranged by size:
2468 2071 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PsrI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI