Restriction map for
pFN21AE0612
(Circular) (Six-base) MAPSORT of: pFN21AE0612.seq Check: 6599 from: 1 to: 5794
pFN21AE0612 5794 bp
With 182 enzymes: SgfI *
December 8, 2014 13:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3604 278
Size: 53 83 186 936 2068 2468
Fragments arranged by size:
2468 2068 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3137 1937
Size: 1200 4594
Acc65I G'GTAC_C
Cuts at: 3115 3115
Size: 5794
AclI AA'CG_TT
Cuts at: 1563 3921 4294 1563
Size: 2358 373 3063
Fragments arranged by size:
3063 2358 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3857 5241 5509 1632
Size: 2225 1384 268 1917
Fragments arranged by size:
2225 1917 1384 268
AfeI AGC'GCT
Cuts at: 2876 2876
Size: 5794
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5597
AflIII A'CryG_T
Cuts at: 1203 4693 5670 1203
Size: 3490 977 1327
Fragments arranged by size:
3490 1327 977
AgeI A'CCGG_T
Cuts at: 2749 4625 2749
Size: 1876 3918
AhdI GACnn_n'nnGTC
Cuts at: 4523 4523
Size: 5794
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5794
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5794
AlwNI CAG_nnn'CTG
Cuts at: 2185 2774 3537 5109 2185
Size: 589 763 1572 2870
Fragments arranged by size:
2870 1572 763 589
ApaI G_GGCC'C
Cuts at: 2066 2358 2681 3088 2066
Size: 292 323 407 4772
Fragments arranged by size:
4772 407 323 292
ApaLI G'TGCA_C
Cuts at: 3851 5007 3851
Size: 1156 4638
ApoI r'AATT_y
Cuts at: 1493 2840 3103 3329 3481 5590 1493
Size: 1347 263 226 152 2109 1697
Fragments arranged by size:
2109 1697 1347 263 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4348 160
Size: 872 3316 1606
Fragments arranged by size:
3316 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5794
AvaI C'yCGr_G
Cuts at: 1870 1943 2065 2414 2472 3119 1870
Size: 73 122 349 58 647 4545
Fragments arranged by size:
4545 647 349 122 73 58
BamHI G'GATC_C
Cuts at: 3124 3491 3124
Size: 367 5427
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2746 2793 3115 4570 618
Size: 325 217 627 959 47 322 1455 1842
Fragments arranged by size:
1842 1455 959 627 325 322 217 47
BanII G_rGCy'C
Cuts at: 1391 2066 2358 2367 2493 2630 2681 2689 2997
Size: 675 292 9 126 137 51 8 308
Cuts at: 2997 3088 3113 1391
Size: 91 25 4072
Fragments arranged by size:
4072 675 308 292 137 126 91 51
25 9 8
BbeI G_GCGC'C
Cuts at: 2797 2797
Size: 5794
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4823
BbvCI CC'TCA_GC
Cuts at: 2736 2736
Size: 5794
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3985 4019 1903
Size: 34 50 34 1964 34 3678
Fragments arranged by size:
3678 1964 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3686 4896 3686
Size: 1210 4584
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5794
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2161 4405 136
Size: 107 122 71 631 605 489 2244 1525
Fragments arranged by size:
2244 1525 631 605 489 122 107 71
BglII A'GATC_T
Cuts at: 2922 5789 2922
Size: 2867 2927
BlpI GC'TnA_GC
Cuts at: 3211 3211
Size: 5794
Bme1580I G_kGCm'C
Cuts at: 1790 2066 2072 2292 2358 2681 2749 3088 3855
Size: 276 6 220 66 323 68 339 767
Cuts at: 3855 5011 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 767 339 323 276 220 68
66 6
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5794
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3525 4483 442
Size: 416 53 83 399 24 2108 958 1753
Fragments arranged by size:
2108 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2234 1056
Size: 1178 4616
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2600 2751 4454 1239
Size: 1361 151 1703 2579
Fragments arranged by size:
2579 1703 1361 151
Bpu10I CC'TnA_GC
Cuts at: 2629 2736 2861 2629
Size: 107 125 5562
Fragments arranged by size:
5562 125 107
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2882 3919 4784 5082 2882
Size: 1037 865 298 3594
Fragments arranged by size:
3594 1037 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2589 4457 882
Size: 617 1090 1868 2219
Fragments arranged by size:
2219 1868 1090 617
BsaAI yAC'GTr
Cuts at: 493 1204 3585 493
Size: 711 2381 2702
Fragments arranged by size:
2702 2381 711
BsaBI GATnn'nnATC
Cuts at: 5788 5788
Size: 5794
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2431 2794 3601 3983
Size: 53 83 186 936 898 363 807 382
Cuts at: 3983 4618 275
Size: 635 1451
Fragments arranged by size:
1451 936 898 807 635 382 363 186
83 53
BsaWI w'CCGG_w
Cuts at: 2749 4227 4625 4899 5046 5457 2749
Size: 1478 398 274 147 411 3086
Fragments arranged by size:
3086 1478 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5569
Fragments arranged by size:
5569 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2585 2753 1186
Size: 1399 168 4227
Fragments arranged by size:
4227 1399 168
BseYI C'CCAG_C
Cuts at: 2275 2321 2599 4997 5493 2275
Size: 46 278 2398 496 2576
Fragments arranged by size:
2576 2398 496 278 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2933 1163
Size: 573 1197 4024
Fragments arranged by size:
4024 1197 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2165 2248 2428 3244 4005 5033
Size: 275 186 164 83 180 816 761 1028
Cuts at: 5033 1540
Size: 2301
Fragments arranged by size:
2301 1028 816 761 275 186 180 164
83
BsiHKAI G_wGCw'C
Cuts at: 2367 3855 3940 5011 2367
Size: 1488 85 1071 3150
Fragments arranged by size:
3150 1488 1071 85
BsmI GAATG_Cn'
Cuts at: 3305 3398 3305
Size: 93 5701
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2066 2072 2292 2358 2367 2493 2630
Size: 399 276 6 220 66 9 126 137
Cuts at: 2630 2681 2689 2749 2997 3088 3113 3855 3940
Size: 51 8 60 248 91 25 742 85
Cuts at: 3940 5011 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 276 248 220 137
126 91 85 66 60 51 25 9
8 6
BspEI T'CCGG_A
Cuts at: 5457 5457
Size: 5794
BspHI T'CATG_A
Cuts at: 3683 5413 3683
Size: 1730 4064
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2905 3149 844
Size: 2061 244 3489
Fragments arranged by size:
3489 2061 244
BsrBI CCG'CTC
Cuts at: 1394 1823 2167 3681 1394
Size: 429 344 1514 3507
Fragments arranged by size:
3507 1514 429 344
BsrDI GCAATG_nn'
Cuts at: 65 1248 2845 2977 4289 4463 65
Size: 1183 1597 132 1312 174 1396
Fragments arranged by size:
1597 1396 1312 1183 174 132
BsrFI r'CCGG_y
Cuts at: 1700 1810 2135 2160 2243 2467 2749 4438 4625
Size: 110 325 25 83 224 282 1689 187
Cuts at: 4625 1700
Size: 2869
Fragments arranged by size:
2869 1689 325 282 224 187 110 83
25
BsrGI T'GTAC_A
Cuts at: 96 2130 2556 96
Size: 2034 426 3334
Fragments arranged by size:
3334 2034 426
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5794
BssSI C'ACGA_G
Cuts at: 2168 3854 4866 2168
Size: 1686 1012 3096
Fragments arranged by size:
3096 1686 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5794
BstBI TT'CG_AA
Cuts at: 4598 4598
Size: 5794
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5794
BstYI r'GATC_y
Cuts at: 1972 2922 3124 3491 3876 3893 5345 5431 5443
Size: 950 202 367 385 17 1452 86 12
Cuts at: 5443 5789 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 950 385 367 346 202 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5242
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5055
Fragments arranged by size:
5055 683 56
BtsI GCAGTG_nn'
Cuts at: 2070 2394 3310 4104 4124 2070
Size: 324 916 794 20 3740
Fragments arranged by size:
3740 916 794 324 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5794
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5759
DraI TTT'AAA
Cuts at: 3098 3098
Size: 5794
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4801 809
Size: 507 3485 1802
Fragments arranged by size:
3485 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2119 2162 2245
Size: 54 1425 50 275 18 289 43 83
Cuts at: 2245 2425 3241 4130 5759 8
Size: 180 816 889 1629 43
Fragments arranged by size:
1629 1425 889 816 289 275 180 83
54 50 43 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2162 2245 2425 3241 1537
Size: 275 350 83 180 816 4090
Fragments arranged by size:
4090 816 350 275 180 83
EarI CTCTTCn'nnn_
Cuts at: 1353 2616 2694 3724 1353
Size: 1263 78 1030 3423
Fragments arranged by size:
3423 1263 1030 78
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2493 4381 4755 4901 387
Size: 1064 138 904 1888 374 146 1280
Fragments arranged by size:
1888 1280 1064 904 374 146 138
EcoICRI GAG'CTC
Cuts at: 2365 2365
Size: 5794
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2600 2751 3857 4454 5241 5509 1239
Size: 393 968 151 1106 597 787 268 1524
Fragments arranged by size:
1524 1106 968 787 597 393 268 151
EcoO109I rG'GnC_Cy
Cuts at: 2062 2355 2677 2696 3084 2062
Size: 293 322 19 388 4772
Fragments arranged by size:
4772 388 322 293 19
EcoRI G'AATT_C
Cuts at: 3103 3481 3103
Size: 378 5416
FspI TGC'GCA
Cuts at: 4300 4300
Size: 5794
HaeII r_GCGC'y
Cuts at: 1678 2031 2186 2306 2797 2878 4941 1678
Size: 353 155 120 491 81 2063 2531
Fragments arranged by size:
2531 2063 491 353 155 120 81
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4435 4467 4509 4541 1373
Size: 32 135 32 2863 32 42 32 2626
Fragments arranged by size:
2863 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3040 3138 3384 669
Size: 988 281 1102 98 246 3079
Fragments arranged by size:
3079 1102 988 281 246 98
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5794
HpaI GTT'AAC
Cuts at: 3384 3384
Size: 5794
KasI G'GCGC_C
Cuts at: 2793 2793
Size: 5794
KpnI G_GTAC'C
Cuts at: 3119 3119
Size: 5794
MfeI C'AATT_G
Cuts at: 3393 3393
Size: 5794
MluI A'CGCG_T
Cuts at: 5670 5670
Size: 5794
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4908 5092 1305
Size: 3603 184 2007
Fragments arranged by size:
3603 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3972
Fragments arranged by size:
3972 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2150 3029 3752 4111 4270 518
Size: 648 984 879 723 359 159 2042
Fragments arranged by size:
2042 984 879 723 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2011 2200 2207 2378 3210 3887 5035 5280
Size: 405 189 7 171 832 677 1148 245
Cuts at: 5280 1606
Size: 2120
Fragments arranged by size:
2120 1148 832 677 405 245 189 171
7
NaeI GCC'GGC
Cuts at: 2245 2469 2245
Size: 224 5570
NarI GG'CG_CC
Cuts at: 2794 2794
Size: 5794
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5242
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5794
NgoMIV G'CCGG_C
Cuts at: 2243 2467 2243
Size: 224 5570
NheI G'CTAG_C
Cuts at: 1052 2230 1052
Size: 1178 4616
NotI GC'GGCC_GC
Cuts at: 3241 3241
Size: 5794
NspI r_CATG'y
Cuts at: 1132 1229 3038 3152 4697 1132
Size: 97 1809 114 1545 2229
Fragments arranged by size:
2229 1809 1545 114 97
PasI CC'CwG_GG
Cuts at: 1348 2033 2681 1348
Size: 685 648 4461
Fragments arranged by size:
4461 685 648
PciI A'CATG_T
Cuts at: 4693 4693
Size: 5794
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2441 3073 1108
Size: 1333 632 3829
Fragments arranged by size:
3829 1333 632
PfoI T'CCnGG_A
Cuts at: 2658 2658
Size: 5794
PmeI GTTT'AAAC
Cuts at: 3098 3098
Size: 5794
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2968 3000 3863 3895 5376 5408 2968
Size: 32 863 32 1481 32 3354
Fragments arranged by size:
3354 1481 863 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2696 2696
Size: 5794
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5794
PsiI TTA'TAA
Cuts at: 3364 3364
Size: 5794
PspOMI G'GGCC_C
Cuts at: 2062 2354 2677 3084 2062
Size: 292 323 407 4772
Fragments arranged by size:
4772 407 323 292
PstI C_TGCA'G
Cuts at: 830 2674 3146 830
Size: 1844 472 3478
Fragments arranged by size:
3478 1844 472
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5794
SacI G_AGCT'C
Cuts at: 2367 2367
Size: 5794
SalI G'TCGA_C
Cuts at: 1936 3136 1936
Size: 1200 4594
SapI GCTCTTCn'nnn_
Cuts at: 2694 2694
Size: 5794
SbfI CC_TGCA'GG
Cuts at: 3146 3146
Size: 5794
ScaI AGT'ACT
Cuts at: 4042 5754 4042
Size: 1712 4082
SfcI C'TryA_G
Cuts at: 826 1045 2670 3142 4277 4958 5149 826
Size: 219 1625 472 1135 681 191 1471
Fragments arranged by size:
1625 1471 1135 681 472 219 191
SfoI GGC'GCC
Cuts at: 2795 2795
Size: 5794
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5794
SgrAI Cr'CCGG_yG
Cuts at: 1700 2749 1700
Size: 1049 4745
SmaI CCC'GGG
Cuts at: 1872 2067 2474 3121 1872
Size: 195 407 647 4545
Fragments arranged by size:
4545 647 407 195
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2897 3898 4799 5061 820
Size: 197 926 954 1001 901 262 1553
Fragments arranged by size:
1553 1001 954 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3585 493
Size: 3092 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5794
SphI G_CATG'C
Cuts at: 1132 3152 1132
Size: 2020 3774
SrfI GCCC'GGGC
Cuts at: 2067 2067
Size: 5794
SspI AAT'ATT
Cuts at: 5 52 3718 5
Size: 47 3666 2081
Fragments arranged by size:
3666 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5794
StyI C'CwwG_G
Cuts at: 513 1065 3233 513
Size: 552 2168 3074
Fragments arranged by size:
3074 2168 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3821 3838 3991 4176 4621 325
Size: 1255 2241 17 153 185 445 1498
Fragments arranged by size:
2241 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2130 2556 4040
Size: 274 80 33 51 1443 153 426 1484
Cuts at: 4040 5752 96
Size: 1712 138
Fragments arranged by size:
1712 1484 1443 426 274 153 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5754 5786 5754
Size: 32 5762
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5794
XbaI T'CTAG_A
Cuts at: 3130 3130
Size: 5794
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5794
XmaI C'CCGG_G
Cuts at: 1870 2065 2472 3119 1870
Size: 195 407 647 4545
Fragments arranged by size:
4545 647 407 195
XmnI GAAnn'nnTTC
Cuts at: 3923 3923
Size: 5794
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3602 276
Size: 53 83 186 936 2068 2468
Fragments arranged by size:
2468 2068 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI
FseI FspAI NruI NsiI PacI PmlI PspXI PsrI
PvuII RsrII SacII SanDI SexAI SfiI SwaI XcmI