Restriction map for
pFN21AE0606
(Circular) (Six-base) MAPSORT of: pFN21AE0606.seq Check: 7626 from: 1 to: 6064
pFN21AE0606 6064 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2734 3064 2734
Size: 330 5734
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3874 278
Size: 53 83 186 936 2338 2468
Fragments arranged by size:
2468 2338 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2694 3407 1937
Size: 757 713 4594
Fragments arranged by size:
4594 757 713
Acc65I G'GTAC_C
Cuts at: 3385 3385
Size: 6064
AclI AA'CG_TT
Cuts at: 1563 4191 4564 1563
Size: 2628 373 3063
Fragments arranged by size:
3063 2628 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2410 4127 5511 5779 1632
Size: 778 1717 1384 268 1917
Fragments arranged by size:
1917 1717 1384 778 268
AfeI AGC'GCT
Cuts at: 2402 2402
Size: 6064
AflII C'TTAA_G
Cuts at: 820 1017 3232 820
Size: 197 2215 3652
Fragments arranged by size:
3652 2215 197
AflIII A'CryG_T
Cuts at: 1203 4963 5940 1203
Size: 3760 977 1327
Fragments arranged by size:
3760 1327 977
AgeI A'CCGG_T
Cuts at: 4895 4895
Size: 6064
AhdI GACnn_n'nnGTC
Cuts at: 4793 4793
Size: 6064
AleI CACnn'nnGTG
Cuts at: 1166 2171 1166
Size: 1005 5059
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6064
AlwNI CAG_nnn'CTG
Cuts at: 2034 2176 2652 3079 3807 5379 2034
Size: 142 476 427 728 1572 2719
Fragments arranged by size:
2719 1572 728 476 427 142
ApaLI G'TGCA_C
Cuts at: 4121 5277 4121
Size: 1156 4908
ApoI r'AATT_y
Cuts at: 1493 3373 3599 3751 5860 1493
Size: 1880 226 152 2109 1697
Fragments arranged by size:
2109 1880 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4618 160
Size: 872 3586 1606
Fragments arranged by size:
3586 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6064
AvaI C'yCGr_G
Cuts at: 1870 1943 2023 2066 2609 3287 3389 1870
Size: 73 80 43 543 678 102 4545
Fragments arranged by size:
4545 678 543 102 80 73 43
BamHI G'GATC_C
Cuts at: 3394 3761 3394
Size: 367 5697
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2681 3195 3385 4840 618
Size: 325 217 627 894 514 190 1455 1842
Fragments arranged by size:
1842 1455 894 627 514 325 217 190
BanII G_rGCy'C
Cuts at: 1391 2771 3261 3303 3383 1391
Size: 1380 490 42 80 4072
Fragments arranged by size:
4072 1380 490 80 42
BbeI G_GCGC'C
Cuts at: 2685 3199 2685
Size: 514 5550
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5093
BbvCI CC'TCA_GC
Cuts at: 2801 3008 2801
Size: 207 5857
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3038 3072 4255 4289 1903
Size: 34 1101 34 1183 34 3678
Fragments arranged by size:
3678 1183 1101 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2183 3956 5166 2183
Size: 1773 1210 3081
Fragments arranged by size:
3081 1773 1210
BclI T'GATC_A
Cuts at: 1548 2050 2118 1548
Size: 502 68 5494
Fragments arranged by size:
5494 502 68
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2025 2501 2766
Size: 107 122 71 631 605 353 476 265
Cuts at: 2766 4675 136
Size: 1909 1525
Fragments arranged by size:
1909 1525 631 605 476 353 265 122
107 71
BglII A'GATC_T
Cuts at: 6059 6059
Size: 6064
BlpI GC'TnA_GC
Cuts at: 3481 3481
Size: 6064
Bme1580I G_kGCm'C
Cuts at: 1790 2556 2786 4125 5281 1790
Size: 766 230 1339 1156 2573
Fragments arranged by size:
2573 1339 1156 766 230
BmgBI CAC'GTC
Cuts at: 1318 2307 1318
Size: 989 5075
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3256 3268 3795
Size: 416 53 83 399 24 1839 12 527
Cuts at: 3795 4753 442
Size: 958 1753
Fragments arranged by size:
1839 1753 958 527 416 399 83 53
24 12
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6064
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3322 3322
Size: 6064
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2569 2629 4724 1239
Size: 1330 60 2095 2579
Fragments arranged by size:
2579 2095 1330 60
Bpu10I CC'TnA_GC
Cuts at: 2801 3008 2801
Size: 207 5857
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4189 5054 5352 4189
Size: 865 298 4901
Fragments arranged by size:
4901 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2573 2606 4727 882
Size: 617 1074 33 2121 2219
Fragments arranged by size:
2219 2121 1074 617 33
BsaAI yAC'GTr
Cuts at: 493 1204 2380 3855 493
Size: 711 1176 1475 2702
Fragments arranged by size:
2702 1475 1176 711
BsaBI GATnn'nnATC
Cuts at: 2093 6058 2093
Size: 3965 2099
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2682 3196 3871 4253
Size: 53 83 186 936 1149 514 675 382
Cuts at: 4253 4888 275
Size: 635 1451
Fragments arranged by size:
1451 1149 936 675 635 514 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4497 4895 5169 5316 5727 4497
Size: 398 274 147 411 4834
Fragments arranged by size:
4834 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2004 2034 2776 2806 1178
Size: 30 165 30 601 30 742 30 4436
Fragments arranged by size:
4436 742 601 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2969 1186
Size: 1783 4281
BseYI C'CCAG_C
Cuts at: 2015 2555 5267 5763 2015
Size: 540 2712 496 2316
Fragments arranged by size:
2712 2316 540 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2318 2360 3092 3188 1163
Size: 573 582 42 732 96 4039
Fragments arranged by size:
4039 732 582 573 96 42
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3514 4275 5303 1540
Size: 275 186 1513 761 1028 2301
Fragments arranged by size:
2301 1513 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2751 4125 4210 5281 2751
Size: 1374 85 1071 3534
Fragments arranged by size:
3534 1374 1071 85
BsmI GAATG_Cn'
Cuts at: 3575 3668 3575
Size: 93 5971
BsmBI CGTCTCn'nnnn_
Cuts at: 2987 2987
Size: 6064
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2556 2751 2771 2786 3261 3303 3383
Size: 399 766 195 20 15 475 42 80
Cuts at: 3383 4125 4210 5281 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 766 742 475 399 195 85
80 42 20 15
BspEI T'CCGG_A
Cuts at: 5727 5727
Size: 6064
BspHI T'CATG_A
Cuts at: 2751 3953 5683 2751
Size: 1202 1730 3132
Fragments arranged by size:
3132 1730 1202
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2734 3064 3419 844
Size: 1890 330 355 3489
Fragments arranged by size:
3489 1890 355 330
BsrBI CCG'CTC
Cuts at: 1394 1823 2583 3028 3951 1394
Size: 429 760 445 923 3507
Fragments arranged by size:
3507 923 760 445 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4559 4733 65
Size: 1183 3311 174 1396
Fragments arranged by size:
3311 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4708 4895 1700
Size: 110 2898 187 2869
Fragments arranged by size:
2898 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3033 96
Size: 2937 3127
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6064
BssSI C'ACGA_G
Cuts at: 2600 2699 3113 4124 5136 2600
Size: 99 414 1011 1012 3528
Fragments arranged by size:
3528 1012 1011 414 99
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2034 794
Size: 1240 4824
BstBI TT'CG_AA
Cuts at: 4868 4868
Size: 6064
BstEII G'GTnAC_C
Cuts at: 1184 2164 2485 1184
Size: 980 321 4763
Fragments arranged by size:
4763 980 321
BstYI r'GATC_y
Cuts at: 1972 3394 3761 4146 4163 5615 5701 5713 6059
Size: 1422 367 385 17 1452 86 12 346
Cuts at: 6059 1972
Size: 1977
Fragments arranged by size:
1977 1452 1422 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2858 2858
Size: 6064
BtgI C'CryG_G
Cuts at: 513 1065 2004 2268 2523 513
Size: 552 939 264 255 4054
Fragments arranged by size:
4054 939 552 264 255
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2392 487
Size: 683 56 1166 4159
Fragments arranged by size:
4159 1166 683 56
BtsI GCAGTG_nn'
Cuts at: 2035 2504 3580 4374 4394 2035
Size: 469 1076 794 20 3705
Fragments arranged by size:
3705 1076 794 469 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6064
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6029
DraI TTT'AAA
Cuts at: 3368 3368
Size: 6064
DraIII CAC_nnn'GTG
Cuts at: 2221 2221
Size: 6064
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5071 809
Size: 507 3755 1802
Fragments arranged by size:
3755 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2079 2775 2880
Size: 54 1425 50 275 18 249 696 105
Cuts at: 2880 2925 3511 4400 6029 8
Size: 45 586 889 1629 43
Fragments arranged by size:
1629 1425 889 696 586 275 249 105
54 50 45 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3511 1537
Size: 275 1699 4090
Fragments arranged by size:
4090 1699 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2351 2814 3188 3994 1353
Size: 998 463 374 806 3423
Fragments arranged by size:
3423 998 806 463 374
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2482 4651 5025 5171 387
Size: 1064 138 893 2169 374 146 1280
Fragments arranged by size:
2169 1280 1064 893 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2410 2569 2629 4127 4724 5511 5779
Size: 393 778 159 60 1498 597 787 268
Cuts at: 5779 1239
Size: 1524
Fragments arranged by size:
1524 1498 787 778 597 393 268 159
60
EcoNI CCTnn'n_nnAGG
Cuts at: 2348 3208 2348
Size: 860 5204
EcoO109I rG'GnC_Cy
Cuts at: 2447 2952 2447
Size: 505 5559
EcoRI G'AATT_C
Cuts at: 3373 3751 3373
Size: 378 5686
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3247 3247
Size: 6064
FspI TGC'GCA
Cuts at: 3138 4570 3138
Size: 1432 4632
FspAI rTGC'GCAy
Cuts at: 3138 3138
Size: 6064
HaeII r_GCGC'y
Cuts at: 1678 2154 2404 2685 3124 3159 3175 3199 5211
Size: 476 250 281 439 35 16 24 2012
Cuts at: 5211 1678
Size: 2531
Fragments arranged by size:
2531 2012 476 439 281 250 35 24
16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3248 3280 4705 4737 4779
Size: 32 135 32 1676 32 1425 32 42
Cuts at: 4779 4811 1373
Size: 32 2626
Fragments arranged by size:
2626 1676 1425 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3408 3654 669
Size: 988 281 1470 246 3079
Fragments arranged by size:
3079 1470 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6064
HpaI GTT'AAC
Cuts at: 3654 3654
Size: 6064
KasI G'GCGC_C
Cuts at: 2681 3195 2681
Size: 514 5550
KpnI G_GTAC'C
Cuts at: 3389 3389
Size: 6064
MfeI C'AATT_G
Cuts at: 3663 3663
Size: 6064
MluI A'CGCG_T
Cuts at: 5940 5940
Size: 6064
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5178 5362 1305
Size: 3873 184 2007
Fragments arranged by size:
3873 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2081 10
Size: 54 1425 343 249 3993
Fragments arranged by size:
3993 1425 343 249 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2058 2126 2171 2246 2261 2267 4022
Size: 648 892 68 45 75 15 6 1755
Cuts at: 4022 4381 4540 518
Size: 359 159 2042
Fragments arranged by size:
2042 1755 892 648 359 159 75 68
45 15 6
MspA1I CmG'CkG
Cuts at: 1606 2652 2800 3480 4157 5305 5550 1606
Size: 1046 148 680 677 1148 245 2120
Fragments arranged by size:
2120 1148 1046 680 677 245 148
NarI GG'CG_CC
Cuts at: 2682 3196 2682
Size: 514 5550
NcoI C'CATG_G
Cuts at: 513 1065 2004 2268 2523 513
Size: 552 939 264 255 4054
Fragments arranged by size:
4054 939 552 264 255
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6064
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6064
NotI GC'GGCC_GC
Cuts at: 3511 3511
Size: 6064
NspI r_CATG'y
Cuts at: 1132 1229 2266 3137 3422 4967 1132
Size: 97 1037 871 285 1545 2229
Fragments arranged by size:
2229 1545 1037 871 285 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6064
PciI A'CATG_T
Cuts at: 4963 4963
Size: 6064
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2652 1108
Size: 1544 4520
PmeI GTTT'AAAC
Cuts at: 3368 3368
Size: 6064
PmlI CAC'GTG
Cuts at: 2380 2380
Size: 6064
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4133 4165 5646 5678 4133
Size: 32 1481 32 4519
Fragments arranged by size:
4519 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2447 2952 2447
Size: 505 5559
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6064
PsiI TTA'TAA
Cuts at: 3634 3634
Size: 6064
PstI C_TGCA'G
Cuts at: 830 2181 2360 3102 3337 3416 830
Size: 1351 179 742 235 79 3478
Fragments arranged by size:
3478 1351 742 235 179 79
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6064
PvuII CAG'CTG
Cuts at: 2800 2800
Size: 6064
SalI G'TCGA_C
Cuts at: 1936 3406 1936
Size: 1470 4594
SapI GCTCTTCn'nnn_
Cuts at: 3188 3188
Size: 6064
SbfI CC_TGCA'GG
Cuts at: 3416 3416
Size: 6064
ScaI AGT'ACT
Cuts at: 4312 6024 4312
Size: 1712 4352
SfcI C'TryA_G
Cuts at: 826 1045 2177 2278 2356 3098 3333 3412 4547
Size: 219 1132 101 78 742 235 79 1135
Cuts at: 4547 5228 5419 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1132 742 681 235 219 191
101 79 78
SfoI GGC'GCC
Cuts at: 2683 3197 2683
Size: 514 5550
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6064
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6064
SmaI CCC'GGG
Cuts at: 1872 2025 3391 1872
Size: 153 1366 4545
Fragments arranged by size:
4545 1366 153
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3232 3287 4168 5069 5331 820
Size: 197 926 1289 55 881 901 262 1553
Fragments arranged by size:
1553 1289 926 901 881 262 197 55
SnaBI TAC'GTA
Cuts at: 493 3855 493
Size: 3362 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6064
SphI G_CATG'C
Cuts at: 1132 2266 3422 1132
Size: 1134 1156 3774
Fragments arranged by size:
3774 1156 1134
SspI AAT'ATT
Cuts at: 5 52 3988 5
Size: 47 3936 2081
Fragments arranged by size:
3936 2081 47
StuI AGG'CCT
Cuts at: 1517 2354 1517
Size: 837 5227
StyI C'CwwG_G
Cuts at: 513 1065 2004 2268 2523 3503 513
Size: 552 939 264 255 980 3074
Fragments arranged by size:
3074 980 939 552 264 255
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2029 2183 3004 4091 4108 4261 4446
Size: 1255 449 154 821 1087 17 153 185
Cuts at: 4446 4891 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1087 821 449 445 185 154
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3033 4310 6022
Size: 274 80 33 51 1443 1056 1277 1712
Cuts at: 6022 96
Size: 138
Fragments arranged by size:
1712 1443 1277 1056 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2004 2036 2776 2808 6024 6056 2004
Size: 32 740 32 3216 32 2012
Fragments arranged by size:
3216 2012 740 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2305 2950 1316
Size: 989 645 4430
Fragments arranged by size:
4430 989 645
XbaI T'CTAG_A
Cuts at: 3400 3400
Size: 6064
XhoI C'TCGA_G
Cuts at: 1943 3287 1943
Size: 1344 4720
XmaI C'CCGG_G
Cuts at: 1870 2023 3389 1870
Size: 153 1366 4545
Fragments arranged by size:
4545 1366 153
XmnI GAAnn'nnTTC
Cuts at: 3227 4193 3227
Size: 966 5098
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3872 276
Size: 53 83 186 936 2338 2468
Fragments arranged by size:
2468 2338 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BfrBI BsiWI BstXI
BstZ17I EcoICRI EcoRV FseI NaeI NgoMIV NruI NsiI
PacI PfoI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SexAI SfiI SrfI SwaI XcmI