Restriction map for
pFN21AE0602
(Circular) (Six-base) MAPSORT of: pFN21AE0602.seq Check: 184 from: 1 to: 5779
pFN21AE0602 5779 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3589 278
Size: 53 83 186 936 2053 2468
Fragments arranged by size:
2468 2053 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3122 1937
Size: 1185 4594
Acc65I G'GTAC_C
Cuts at: 2299 2505 3100 2299
Size: 206 595 4978
Fragments arranged by size:
4978 595 206
AclI AA'CG_TT
Cuts at: 1563 3906 4279 1563
Size: 2343 373 3063
Fragments arranged by size:
3063 2343 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2200 2695 2757 3842 5226 5494 1632
Size: 568 495 62 1085 1384 268 1917
Fragments arranged by size:
1917 1384 1085 568 495 268 62
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5582
AflIII A'CryG_T
Cuts at: 1203 2837 4678 5655 1203
Size: 1634 1841 977 1327
Fragments arranged by size:
1841 1634 1327 977
AgeI A'CCGG_T
Cuts at: 4610 4610
Size: 5779
AhdI GACnn_n'nnGTC
Cuts at: 4508 4508
Size: 5779
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5779
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5779
AlwNI CAG_nnn'CTG
Cuts at: 2338 2735 3522 5094 2338
Size: 397 787 1572 3023
Fragments arranged by size:
3023 1572 787 397
ApaLI G'TGCA_C
Cuts at: 3836 4992 3836
Size: 1156 4623
ApoI r'AATT_y
Cuts at: 1493 2350 2724 2762 3088 3314 3466 5575 1493
Size: 857 374 38 326 226 152 2109 1697
Fragments arranged by size:
2109 1697 857 374 326 226 152 38
AseI AT'TA_AT
Cuts at: 160 1032 4333 160
Size: 872 3301 1606
Fragments arranged by size:
3301 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5779
AvaI C'yCGr_G
Cuts at: 1870 1943 3104 1870
Size: 73 1161 4545
Fragments arranged by size:
4545 1161 73
AvrII C'CTAG_G
Cuts at: 2675 2675
Size: 5779
BamHI G'GATC_C
Cuts at: 2045 3109 3476 2045
Size: 1064 367 4348
Fragments arranged by size:
4348 1064 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2299 2505 2679 3100 4555
Size: 325 217 627 512 206 174 421 1455
Cuts at: 4555 618
Size: 1842
Fragments arranged by size:
1842 1455 627 512 421 325 217 206
174
BanII G_rGCy'C
Cuts at: 1391 2796 3098 1391
Size: 1405 302 4072
Fragments arranged by size:
4072 1405 302
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2206 2660 2930 928
Size: 971 307 454 270 3777
Fragments arranged by size:
3777 971 454 307 270
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3970 4004 1903
Size: 34 2033 34 3678
Fragments arranged by size:
3678 2033 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3671 4881 3671
Size: 1210 4569
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5779
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2611 4390 136
Size: 107 122 71 631 605 939 1779 1525
Fragments arranged by size:
1779 1525 939 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5774 5774
Size: 5779
BlpI GC'TnA_GC
Cuts at: 2586 3196 2586
Size: 610 5169
Bme1580I G_kGCm'C
Cuts at: 1790 3840 4996 1790
Size: 2050 1156 2573
Fragments arranged by size:
2573 2050 1156
BmgBI CAC'GTC
Cuts at: 1318 2386 1318
Size: 1068 4711
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3510 4468 442
Size: 416 53 83 399 24 2093 958 1753
Fragments arranged by size:
2093 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5779
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4439 1239
Size: 3200 2579
Bpu10I CC'TnA_GC
Cuts at: 2517 2517
Size: 5779
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2156 2165 3904 4769 5067 2156
Size: 9 1739 865 298 2868
Fragments arranged by size:
2868 1739 865 298 9
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4442 882
Size: 617 2943 2219
Fragments arranged by size:
2943 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2491 3570 493
Size: 711 1287 1079 2702
Fragments arranged by size:
2702 1287 1079 711
BsaBI GATnn'nnATC
Cuts at: 2631 5773 2631
Size: 3142 2637
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3586 3968 4603 275
Size: 53 83 186 936 2053 382 635 1451
Fragments arranged by size:
2053 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4212 4610 4884 5031 5442 4212
Size: 398 274 147 411 4549
Fragments arranged by size:
4549 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5554
Fragments arranged by size:
5554 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5779
BseYI C'CCAG_C
Cuts at: 4982 5478 4982
Size: 496 5283
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5206
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3229 3990 5018 1540
Size: 275 186 1228 761 1028 2301
Fragments arranged by size:
2301 1228 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3840 3925 4996 3840
Size: 85 1071 4623
Fragments arranged by size:
4623 1071 85
BsmI GAATG_Cn'
Cuts at: 2172 3290 3383 2172
Size: 1118 93 4568
Fragments arranged by size:
4568 1118 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2376 2376
Size: 5779
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2796 3098 3840 3925 4996 1391
Size: 399 1006 302 742 85 1071 2174
Fragments arranged by size:
2174 1071 1006 742 399 302 85
BspEI T'CCGG_A
Cuts at: 5442 5442
Size: 5779
BspHI T'CATG_A
Cuts at: 3668 5398 3668
Size: 1730 4049
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3134 844
Size: 2290 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2511 3666 1394
Size: 429 688 1155 3507
Fragments arranged by size:
3507 1155 688 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4274 4448 65
Size: 1183 3026 174 1396
Fragments arranged by size:
3026 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4423 4610 1700
Size: 110 2613 187 2869
Fragments arranged by size:
2869 2613 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5779
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5779
BssSI C'ACGA_G
Cuts at: 2312 3839 4851 2312
Size: 1527 1012 3240
Fragments arranged by size:
3240 1527 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2338 794
Size: 1544 4235
BstBI TT'CG_AA
Cuts at: 4583 4583
Size: 5779
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5779
BstXI CCAn_nnnn'nTGG
Cuts at: 2996 2996
Size: 5779
BstYI r'GATC_y
Cuts at: 1972 2045 2665 3109 3476 3861 3878 5330 5416
Size: 73 620 444 367 385 17 1452 86
Cuts at: 5416 5428 5774 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 620 444 385 367 346 86
73 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5227
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5040
Fragments arranged by size:
5040 683 56
BtsI GCAGTG_nn'
Cuts at: 2336 2950 3295 4089 4109 2336
Size: 614 345 794 20 4006
Fragments arranged by size:
4006 794 614 345 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5779
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5744
DraI TTT'AAA
Cuts at: 3083 3083
Size: 5779
DraIII CAC_nnn'GTG
Cuts at: 2242 2242
Size: 5779
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4786 809
Size: 507 3470 1802
Fragments arranged by size:
3470 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2097 2612 3226
Size: 54 1425 50 275 18 267 515 614
Cuts at: 3226 4115 5744 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 614 515 275 267 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3226 1537
Size: 275 1414 4090
Fragments arranged by size:
4090 1414 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2312 3709 1353
Size: 959 1397 3423
Fragments arranged by size:
3423 1397 959
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4366 4740 4886 387
Size: 1064 138 2777 374 146 1280
Fragments arranged by size:
2777 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2200 2695 2757 3842 4439 5226 5494
Size: 393 568 495 62 1085 597 787 268
Cuts at: 5494 1239
Size: 1524
Fragments arranged by size:
1524 1085 787 597 568 495 393 268
62
EcoNI CCTnn'n_nnAGG
Cuts at: 2987 2987
Size: 5779
EcoRI G'AATT_C
Cuts at: 3088 3466 3088
Size: 378 5401
FspI TGC'GCA
Cuts at: 4285 4285
Size: 5779
HaeII r_GCGC'y
Cuts at: 1678 4926 1678
Size: 3248 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2579 2611 4420 4452 4494
Size: 32 135 32 1007 32 1809 32 42
Cuts at: 4494 4526 1373
Size: 32 2626
Fragments arranged by size:
2626 1809 1007 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2200 3123 3369 669
Size: 988 281 262 923 246 3079
Fragments arranged by size:
3079 988 923 281 262 246
HindIII A'AGCT_T
Cuts at: 748 2429 748
Size: 1681 4098
HpaI GTT'AAC
Cuts at: 2200 3369 2200
Size: 1169 4610
KpnI G_GTAC'C
Cuts at: 2303 2509 3104 2303
Size: 206 595 4978
Fragments arranged by size:
4978 595 206
MfeI C'AATT_G
Cuts at: 2368 2568 3378 2368
Size: 200 810 4769
Fragments arranged by size:
4769 810 200
MluI A'CGCG_T
Cuts at: 5655 5655
Size: 5779
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2259 4893 5077 1305
Size: 954 2634 184 2007
Fragments arranged by size:
2634 2007 954 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2099 2614 10
Size: 54 1425 343 267 515 3175
Fragments arranged by size:
3175 1425 515 343 267 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3737 4096 4255 518
Size: 648 2571 359 159 2042
Fragments arranged by size:
2571 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2638 2848 3195 3872 5020 5265 1606
Size: 1032 210 347 677 1148 245 2120
Fragments arranged by size:
2120 1148 1032 677 347 245 210
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5227
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5779
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5779
NotI GC'GGCC_GC
Cuts at: 3226 3226
Size: 5779
NspI r_CATG'y
Cuts at: 1132 1229 2841 3137 4682 1132
Size: 97 1612 296 1545 2229
Fragments arranged by size:
2229 1612 1545 296 97
PasI CC'CwG_GG
Cuts at: 1348 2788 1348
Size: 1440 4339
PciI A'CATG_T
Cuts at: 2837 4678 2837
Size: 1841 3938
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2278 1108
Size: 1170 4609
PfoI T'CCnGG_A
Cuts at: 2205 2205
Size: 5779
PmeI GTTT'AAAC
Cuts at: 3083 3083
Size: 5779
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3848 3880 5361 5393 3848
Size: 32 1481 32 4234
Fragments arranged by size:
4234 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2085 1613
Size: 472 5307
PsiI TTA'TAA
Cuts at: 3349 3349
Size: 5779
PstI C_TGCA'G
Cuts at: 830 3131 830
Size: 2301 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5779
PvuII CAG'CTG
Cuts at: 2638 2848 2638
Size: 210 5569
SalI G'TCGA_C
Cuts at: 1936 3121 1936
Size: 1185 4594
SapI GCTCTTCn'nnn_
Cuts at: 2312 2312
Size: 5779
SbfI CC_TGCA'GG
Cuts at: 3131 3131
Size: 5779
ScaI AGT'ACT
Cuts at: 2708 3035 4027 5739 2708
Size: 327 992 1712 2748
Fragments arranged by size:
2748 1712 992 327
SexAI A'CCwGG_T
Cuts at: 2238 2498 2238
Size: 260 5519
SfcI C'TryA_G
Cuts at: 826 1045 3127 4262 4943 5134 826
Size: 219 2082 1135 681 191 1471
Fragments arranged by size:
2082 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5779
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5779
SmaI CCC'GGG
Cuts at: 1872 3106 1872
Size: 1234 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2171 2180 3883 4784 5046 820
Size: 197 926 228 9 1703 901 262 1553
Fragments arranged by size:
1703 1553 926 901 262 228 197 9
SnaBI TAC'GTA
Cuts at: 493 3570 493
Size: 3077 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5779
SphI G_CATG'C
Cuts at: 1132 3137 1132
Size: 2005 3774
SspI AAT'ATT
Cuts at: 5 52 2252 3703 5
Size: 47 2200 1451 2081
Fragments arranged by size:
2200 2081 1451 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5779
StyI C'CwwG_G
Cuts at: 513 1065 2658 2675 3218 513
Size: 552 1593 17 543 3074
Fragments arranged by size:
3074 1593 552 543 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2482 2874 3806 3823 3976 4161 4606
Size: 1255 902 392 932 17 153 185 445
Cuts at: 4606 325
Size: 1498
Fragments arranged by size:
1498 1255 932 902 445 392 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2706 3033 4025
Size: 274 80 33 51 1443 729 327 992
Cuts at: 4025 5737 96
Size: 1712 138
Fragments arranged by size:
1712 1443 992 729 327 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5739 5771 5739
Size: 32 5747
Tth111I GACn'n_nGTC
Cuts at: 1316 2132 1316
Size: 816 4963
XbaI T'CTAG_A
Cuts at: 3115 3115
Size: 5779
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5779
XmaI C'CCGG_G
Cuts at: 1870 3104 1870
Size: 1234 4545
XmnI GAAnn'nnTTC
Cuts at: 3908 3908
Size: 5779
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3587 276
Size: 53 83 186 936 2053 2468
Fragments arranged by size:
2468 2053 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SalI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BplI BsiWI BstZ17I Bsu36I EcoICRI EcoO109I EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI