Restriction map for
pFN21AE0597
(Circular) (Six-base) MAPSORT of: pFN21AE0597.seq Check: 78 from: 1 to: 9154
pFN21AE0597 9154 bp
With 182 enzymes: SgfI *
August 20, 2012 11:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3181 3181
Size: 9154
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4229 6964 278
Size: 53 83 186 936 2693 2735 2468
Fragments arranged by size:
2735 2693 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6497 1937
Size: 4560 4594
Acc65I G'GTAC_C
Cuts at: 5076 6475 5076
Size: 1399 7755
AclI AA'CG_TT
Cuts at: 1563 7281 7654 1563
Size: 5718 373 3063
Fragments arranged by size:
5718 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3111 3308 3642 3822 4530 4638 5288 5763
Size: 1479 197 334 180 708 108 650 475
Cuts at: 5763 5984 6067 6079 6411 7217 8601 8869 1632
Size: 221 83 12 332 806 1384 268 1917
Fragments arranged by size:
1917 1479 1384 806 708 650 475 334
332 268 221 197 180 108 83 12
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8957
AflIII A'CryG_T
Cuts at: 1203 8053 9030 1203
Size: 6850 977 1327
Fragments arranged by size:
6850 1327 977
AgeI A'CCGG_T
Cuts at: 7985 7985
Size: 9154
AhdI GACnn_n'nnGTC
Cuts at: 7883 7883
Size: 9154
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9154
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4338 4559 6293 1184
Size: 3154 221 1734 4045
Fragments arranged by size:
4045 3154 1734 221
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4560 4592 6167 6199 4560
Size: 32 1575 32 7515
Fragments arranged by size:
7515 1575 32 32
AlwNI CAG_nnn'CTG
Cuts at: 4508 4652 4844 6897 8469 4508
Size: 144 192 2053 1572 5193
Fragments arranged by size:
5193 2053 1572 192 144
ApaLI G'TGCA_C
Cuts at: 7211 8367 7211
Size: 1156 7998
ApoI r'AATT_y
Cuts at: 1493 2651 3076 3262 3370 3848 4211 5119 5240
Size: 1158 425 186 108 478 363 908 121
Cuts at: 5240 5324 6463 6689 6841 8950 1493
Size: 84 1139 226 152 2109 1697
Fragments arranged by size:
2109 1697 1158 1139 908 478 425 363
226 186 152 121 108 84
AseI AT'TA_AT
Cuts at: 160 1032 2574 7708 160
Size: 872 1542 5134 1606
Fragments arranged by size:
5134 1606 1542 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9154
AvaI C'yCGr_G
Cuts at: 1870 1943 2106 5250 5917 6479 1870
Size: 73 163 3144 667 562 4545
Fragments arranged by size:
4545 3144 667 562 163 73
AvrII C'CTAG_G
Cuts at: 3386 3386
Size: 9154
BamHI G'GATC_C
Cuts at: 2936 4269 6484 6851 2936
Size: 1333 2215 367 5239
Fragments arranged by size:
5239 2215 1333 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2008 2465 5076 5913 5959
Size: 325 217 627 221 457 2611 837 46
Cuts at: 5959 6475 7930 618
Size: 516 1455 1842
Fragments arranged by size:
2611 1842 1455 837 627 516 457 325
221 217 46
BanII G_rGCy'C
Cuts at: 1391 2644 3364 3541 4127 4491 6017 6473 1391
Size: 1253 720 177 586 364 1526 456 4072
Fragments arranged by size:
4072 1526 1253 720 586 456 364 177
BbeI G_GCGC'C
Cuts at: 2012 5917 2012
Size: 3905 5249
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3296 3509 4017 4518 5015 5468 6302
Size: 971 1397 213 508 501 497 453 834
Cuts at: 6302 6448 928
Size: 146 3634
Fragments arranged by size:
3634 1397 971 834 508 501 497 453
213 146
BbvCI CC'TCA_GC
Cuts at: 5979 6249 5979
Size: 270 8884
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2574 2608 7345 7379 1903
Size: 34 637 34 4737 34 3678
Fragments arranged by size:
4737 3678 637 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3787 3903 7046 8256 3787
Size: 116 3143 1210 4685
Fragments arranged by size:
4685 3143 1210 116
BclI T'GATC_A
Cuts at: 1548 6141 1548
Size: 4593 4561
BfrBI ATG'CAT
Cuts at: 2786 2786
Size: 9154
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5306 7765 136
Size: 107 122 71 631 605 3634 2459 1525
Fragments arranged by size:
3634 2459 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4097 4245 9149 4097
Size: 148 4904 4102
Fragments arranged by size:
4904 4102 148
BlpI GC'TnA_GC
Cuts at: 2223 6281 6571 2223
Size: 4058 290 4806
Fragments arranged by size:
4806 4058 290
Bme1580I G_kGCm'C
Cuts at: 1790 5690 7215 8371 1790
Size: 3900 1525 1156 2573
Fragments arranged by size:
3900 2573 1525 1156
BmgBI CAC'GTC
Cuts at: 1318 2157 1318
Size: 839 8315
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 6885 7843 442
Size: 416 53 83 399 24 5468 958 1753
Fragments arranged by size:
5468 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9154
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3560 3560
Size: 9154
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2485 4220 5471 5476 6053 7814 1239
Size: 1246 1735 1251 5 577 1761 2579
Fragments arranged by size:
2579 1761 1735 1251 1246 577 5
Bpu10I CC'TnA_GC
Cuts at: 2514 5979 6249 2514
Size: 3465 270 5419
Fragments arranged by size:
5419 3465 270
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2378 3737 3874 3946 7279 8144 8442 2378
Size: 1359 137 72 3333 865 298 3090
Fragments arranged by size:
3333 3090 1359 865 298 137 72
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4196 4298 5845 5952 7817 882
Size: 617 2697 102 1547 107 1865 2219
Fragments arranged by size:
2697 2219 1865 1547 617 107 102
BsaAI yAC'GTr
Cuts at: 493 1204 2588 3022 5503 6945 493
Size: 711 1384 434 2481 1442 2702
Fragments arranged by size:
2702 2481 1442 1384 711 434
BsaBI GATnn'nnATC
Cuts at: 9148 9148
Size: 9154
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 4226 5914 6961
Size: 53 83 186 936 476 2217 1688 1047
Cuts at: 6961 7343 7978 275
Size: 382 635 1451
Fragments arranged by size:
2217 1688 1451 1047 936 635 476 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 4266 7587 7985 8259 8406 8817 4266
Size: 3321 398 274 147 411 4603
Fragments arranged by size:
4603 3321 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2279 2309 2554 2584 3972
Size: 30 165 30 876 30 245 30 1388
Cuts at: 3972 4002 1178
Size: 30 6330
Fragments arranged by size:
6330 1388 876 245 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2075 2252 2634 3493 3674 4247 5804 5807
Size: 889 177 382 859 181 573 1557 3
Cuts at: 5807 5810 5822 5825 5828 1186
Size: 3 12 3 3 4512
Fragments arranged by size:
4512 1557 889 859 573 382 181 177
12 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2858 4785 5689 8357 8853 2858
Size: 1927 904 2668 496 3159
Fragments arranged by size:
3159 2668 1927 904 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3122 3951 5430 6191 6227 1163
Size: 573 1386 829 1479 761 36 4090
Fragments arranged by size:
4090 1479 1386 829 761 573 36
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6604 7365 8393 1540
Size: 275 186 4603 761 1028 2301
Fragments arranged by size:
4603 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2587 3541 3597 6017 7215 7300 8371 2587
Size: 954 56 2420 1198 85 1071 3370
Fragments arranged by size:
3370 2420 1198 1071 954 85 56
BsmI GAATG_Cn'
Cuts at: 3779 4060 6665 6758 3779
Size: 281 2605 93 6175
Fragments arranged by size:
6175 2605 281 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3548 3548
Size: 9154
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2587 2644 3364 3541 3597 4127 4491
Size: 399 797 57 720 177 56 530 364
Cuts at: 4491 5690 6017 6473 7215 7300 8371 1391
Size: 1199 327 456 742 85 1071 2174
Fragments arranged by size:
2174 1199 1071 797 742 720 530 456
399 364 327 177 85 57 56
BspEI T'CCGG_A
Cuts at: 8817 8817
Size: 9154
BspHI T'CATG_A
Cuts at: 2334 4275 7043 8773 2334
Size: 1941 2768 1730 2715
Fragments arranged by size:
2768 2715 1941 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3181 6509 844
Size: 2337 3328 3489
Fragments arranged by size:
3489 3328 2337
BsrBI CCG'CTC
Cuts at: 1394 1823 2014 2068 6201 7041 1394
Size: 429 191 54 4133 840 3507
Fragments arranged by size:
4133 3507 840 429 191 54
BsrDI GCAATG_nn'
Cuts at: 65 1248 3438 7649 7823 65
Size: 1183 2190 4211 174 1396
Fragments arranged by size:
4211 2190 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5650 5910 7798 7985 1700
Size: 110 3840 260 1888 187 2869
Fragments arranged by size:
3840 2869 1888 260 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9154
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9154
BssSI C'ACGA_G
Cuts at: 3357 7214 8226 3357
Size: 3857 1012 4285
Fragments arranged by size:
4285 3857 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5438 5506 5555 794
Size: 4644 68 49 4393
Fragments arranged by size:
4644 4393 68 49
BstBI TT'CG_AA
Cuts at: 7958 7958
Size: 9154
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9154
BstXI CCAn_nnnn'nTGG
Cuts at: 3844 3844
Size: 9154
BstYI r'GATC_y
Cuts at: 1972 2936 4097 4245 4269 6054 6484 6851 7236
Size: 964 1161 148 24 1785 430 367 385
Cuts at: 7236 7253 8705 8791 8803 9149 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1785 1452 1161 964 430 385 367
346 148 86 24 17 12
Bsu36I CC'TnA_GG
Cuts at: 5031 5031
Size: 9154
BtgI C'CryG_G
Cuts at: 513 1065 2004 3365 3837 5725 6153 6265 513
Size: 552 939 1361 472 1888 428 112 3402
Fragments arranged by size:
3402 1888 1361 939 552 472 428 112
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8415
Fragments arranged by size:
8415 683 56
BtsI GCAGTG_nn'
Cuts at: 4822 5690 6670 7464 7484 4822
Size: 868 980 794 20 6492
Fragments arranged by size:
6492 980 868 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9154
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5621 5656 5707 5742 6247 6282 529
Size: 35 5057 35 51 35 505 35 3401
Fragments arranged by size:
5057 3401 505 51 35 35 35 35
DraI TTT'AAA
Cuts at: 5160 6458 5160
Size: 1298 7856
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4838 8161 809
Size: 507 3522 3323 1802
Fragments arranged by size:
3522 3323 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3333 3834 6340
Size: 54 1425 50 275 18 1503 501 2506
Cuts at: 6340 6601 7490 9119 8
Size: 261 889 1629 43
Fragments arranged by size:
2506 1629 1503 1425 889 501 275 261
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6601 1537
Size: 275 4789 4090
Fragments arranged by size:
4789 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2075 2106 2352 2433 2986 3694 4370 4526
Size: 722 31 246 81 553 708 676 156
Cuts at: 4526 4751 5066 5403 5739 5781 5799 6045 7084
Size: 225 315 337 336 42 18 246 1039
Cuts at: 7084 1353
Size: 3423
Fragments arranged by size:
3423 1039 722 708 676 553 337 336
315 246 246 225 156 81 42 31
18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7741 8115 8261 387
Size: 1064 138 6152 374 146 1280
Fragments arranged by size:
6152 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3539 6015 3539
Size: 2476 6678
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2485 3111 3308 3642 3822 4220 4530
Size: 393 853 626 197 334 180 398 310
Cuts at: 4530 4638 5288 5471 5476 5763 5984 6053 6067
Size: 108 650 183 5 287 221 69 14
Cuts at: 6067 6079 6411 7217 7814 8601 8869 1239
Size: 12 332 806 597 787 268 1524
Fragments arranged by size:
1524 853 806 787 650 626 597 398
393 334 332 310 287 268 221 197
183 180 108 69 14 12 5
EcoNI CCTnn'n_nnAGG
Cuts at: 3162 3162
Size: 9154
EcoO109I rG'GnC_Cy
Cuts at: 3390 3390
Size: 9154
EcoRI G'AATT_C
Cuts at: 3076 3262 3370 3848 6463 6841 3076
Size: 186 108 478 2615 378 5389
Fragments arranged by size:
5389 2615 478 378 186 108
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3731 3961 5231 6097 3731
Size: 230 1270 866 6788
Fragments arranged by size:
6788 1270 866 230
FseI GG_CCGG'CC
Cuts at: 5654 5654
Size: 9154
FspI TGC'GCA
Cuts at: 4094 4700 7660 4094
Size: 606 2960 5588
Fragments arranged by size:
5588 2960 606
HaeII r_GCGC'y
Cuts at: 1678 2012 2743 3486 5917 8301 1678
Size: 334 731 743 2431 2384 2531
Fragments arranged by size:
2531 2431 2384 743 731 334
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2441 2465 2473 2497 4211
Size: 32 135 32 869 24 8 24 1714
Cuts at: 4211 4243 4728 4760 7795 7827 7869 7901 1373
Size: 32 485 32 3035 32 42 32 2626
Fragments arranged by size:
3035 2626 1714 869 485 135 42 32
32 32 32 32 32 24 24 8
HincII GTy'rAC
Cuts at: 669 1657 1938 3759 5051 6498 6744 669
Size: 988 281 1821 1292 1447 246 3079
Fragments arranged by size:
3079 1821 1447 1292 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2261 4659 748
Size: 1513 2398 5243
Fragments arranged by size:
5243 2398 1513
HpaI GTT'AAC
Cuts at: 3759 6744 3759
Size: 2985 6169
KasI G'GCGC_C
Cuts at: 2008 5913 2008
Size: 3905 5249
KpnI G_GTAC'C
Cuts at: 5080 6479 5080
Size: 1399 7755
MfeI C'AATT_G
Cuts at: 6753 6753
Size: 9154
MluI A'CGCG_T
Cuts at: 9030 9030
Size: 9154
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2307 3306 6403 8268 8452 1305
Size: 1002 999 3097 1865 184 2007
Fragments arranged by size:
3097 2007 1865 1002 999 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3335 3836 6342 10
Size: 54 1425 343 1503 501 2506 2822
Fragments arranged by size:
2822 2506 1503 1425 501 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2667 3842 6348 7112 7471 7630 518
Size: 648 1501 1175 2506 764 359 159 2042
Fragments arranged by size:
2506 2042 1501 1175 764 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2038 4154 4649 6087 6570 7247 8395 8640
Size: 432 2116 495 1438 483 677 1148 245
Cuts at: 8640 1606
Size: 2120
Fragments arranged by size:
2120 2116 1438 1148 677 495 483 432
245
NaeI GCC'GGC
Cuts at: 5652 5912 5652
Size: 260 8894
NarI GG'CG_CC
Cuts at: 2009 5914 2009
Size: 3905 5249
NcoI C'CATG_G
Cuts at: 513 1065 2004 3837 6153 513
Size: 552 939 1833 2316 3514
Fragments arranged by size:
3514 2316 1833 939 552
NdeI CA'TA_TG
Cuts at: 387 6349 387
Size: 5962 3192
NgoMIV G'CCGG_C
Cuts at: 5650 5910 5650
Size: 260 8894
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9154
NotI GC'GGCC_GC
Cuts at: 6601 6601
Size: 9154
NsiI A_TGCA'T
Cuts at: 2788 2788
Size: 9154
NspI r_CATG'y
Cuts at: 1132 1229 6512 8057 1132
Size: 97 5283 1545 2229
Fragments arranged by size:
5283 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9154
PciI A'CATG_T
Cuts at: 8053 8053
Size: 9154
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 9154
PfoI T'CCnGG_A
Cuts at: 4578 4665 4762 5472 5492 6017 4578
Size: 87 97 710 20 525 7715
Fragments arranged by size:
7715 710 525 97 87 20
PmeI GTTT'AAAC
Cuts at: 6458 6458
Size: 9154
PmlI CAC'GTG
Cuts at: 5503 5503
Size: 9154
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7223 7255 8736 8768 7223
Size: 32 1481 32 7609
Fragments arranged by size:
7609 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3390 3390
Size: 9154
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9154
PsiI TTA'TAA
Cuts at: 6724 6724
Size: 9154
PstI C_TGCA'G
Cuts at: 830 3427 4327 5899 6042 6506 830
Size: 2597 900 1572 143 464 3478
Fragments arranged by size:
3478 2597 1572 900 464 143
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9154
PvuII CAG'CTG
Cuts at: 4154 4649 6087 4154
Size: 495 1438 7221
Fragments arranged by size:
7221 1438 495
SacI G_AGCT'C
Cuts at: 3541 6017 3541
Size: 2476 6678
SalI G'TCGA_C
Cuts at: 1936 6496 1936
Size: 4560 4594
SapI GCTCTTCn'nnn_
Cuts at: 2075 4526 4751 2075
Size: 2451 225 6478
Fragments arranged by size:
6478 2451 225
SbfI CC_TGCA'GG
Cuts at: 6506 6506
Size: 9154
ScaI AGT'ACT
Cuts at: 2167 7402 9114 2167
Size: 5235 1712 2207
Fragments arranged by size:
5235 2207 1712
SfcI C'TryA_G
Cuts at: 826 1045 3423 4241 4323 4938 5895 6038 6502
Size: 219 2378 818 82 615 957 143 464
Cuts at: 6502 7637 8318 8509 826
Size: 1135 681 191 1471
Fragments arranged by size:
2378 1471 1135 957 818 681 615 464
219 191 143 82
SfoI GGC'GCC
Cuts at: 2010 5915 2010
Size: 3905 5249
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9154
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9154
SmaI CCC'GGG
Cuts at: 1872 2108 5252 5919 6481 1872
Size: 236 3144 667 562 4545
Fragments arranged by size:
4545 3144 667 562 236
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2393 3716 3853 3925 7258 8159
Size: 197 926 450 1323 137 72 3333 901
Cuts at: 8159 8421 820
Size: 262 1553
Fragments arranged by size:
3333 1553 1323 926 901 450 262 197
137 72
SnaBI TAC'GTA
Cuts at: 493 6945 493
Size: 6452 2702
SpeI A'CTAG_T
Cuts at: 152 4690 152
Size: 4538 4616
SphI G_CATG'C
Cuts at: 1132 6512 1132
Size: 5380 3774
SspI AAT'ATT
Cuts at: 5 52 3641 7078 5
Size: 47 3589 3437 2081
Fragments arranged by size:
3589 3437 2081 47
StuI AGG'CCT
Cuts at: 1517 2096 1517
Size: 579 8575
StyI C'CwwG_G
Cuts at: 513 1065 2004 2816 3386 3837 4680 5332 5955
Size: 552 939 812 570 451 843 652 623
Cuts at: 5955 6153 6593 513
Size: 198 440 3074
Fragments arranged by size:
3074 939 843 812 652 623 570 552
451 440 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4969 6360 7181 7198 7351 7536 7981
Size: 1255 3389 1391 821 17 153 185 445
Cuts at: 7981 325
Size: 1498
Fragments arranged by size:
3389 1498 1391 1255 821 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2165 7400 9112
Size: 274 80 33 51 1443 188 5235 1712
Cuts at: 9112 96
Size: 138
Fragments arranged by size:
5235 1712 1443 274 188 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3181 3213 9114 9146 3181
Size: 32 5901 32 3189
Fragments arranged by size:
5901 3189 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9154
XbaI T'CTAG_A
Cuts at: 3100 3589 6490 3100
Size: 489 2901 5764
Fragments arranged by size:
5764 2901 489
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2347 3758 4841 5676 2347
Size: 1411 1083 835 5825
Fragments arranged by size:
5825 1411 1083 835
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9154
XmaI C'CCGG_G
Cuts at: 1870 2106 5250 5917 6479 1870
Size: 236 3144 667 562 4545
Fragments arranged by size:
4545 3144 667 562 236
XmnI GAAnn'nnTTC
Cuts at: 2277 4661 7283 2277
Size: 2384 2622 4148
Fragments arranged by size:
4148 2622 2384
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4227 6962 276
Size: 53 83 186 936 2693 2735 2468
Fragments arranged by size:
2735 2693 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI BaeI BsiWI BstZ17I DraIII EcoRV
FspAI NruI PacI PspOMI PspXI PsrI RsrII SacII
SanDI SexAI SfiI SrfI SwaI