Restriction map for
pFN21AE0593
(Circular) (Six-base) MAPSORT of: pFN21AE0593.seq Check: 8582 from: 1 to: 5446
pFN21AE0593 5446 bp
With 182 enzymes: SgfI *
October 20, 2009 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2093 2331 2093
Size: 238 5208
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2594 3256 278
Size: 53 83 186 936 1058 662 2468
Fragments arranged by size:
2468 1058 936 662 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2789 1937
Size: 852 4594
Acc65I G'GTAC_C
Cuts at: 2767 2767
Size: 5446
AclI AA'CG_TT
Cuts at: 1563 3573 3946 1563
Size: 2010 373 3063
Fragments arranged by size:
3063 2010 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3509 4893 5161 1632
Size: 1877 1384 268 1917
Fragments arranged by size:
1917 1877 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5249
AflIII A'CryG_T
Cuts at: 1203 4345 5322 1203
Size: 3142 977 1327
Fragments arranged by size:
3142 1327 977
AgeI A'CCGG_T
Cuts at: 4277 4277
Size: 5446
AhdI GACnn_n'nnGTC
Cuts at: 4175 4175
Size: 5446
AleI CACnn'nnGTG
Cuts at: 1166 2691 1166
Size: 1525 3921
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5446
AlwNI CAG_nnn'CTG
Cuts at: 2150 2495 3189 4761 2150
Size: 345 694 1572 2835
Fragments arranged by size:
2835 1572 694 345
ApaI G_GGCC'C
Cuts at: 2383 2383
Size: 5446
ApaLI G'TGCA_C
Cuts at: 3503 4659 3503
Size: 1156 4290
ApoI r'AATT_y
Cuts at: 1493 2755 2981 3133 5242 1493
Size: 1262 226 152 2109 1697
Fragments arranged by size:
2109 1697 1262 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4000 160
Size: 872 2968 1606
Fragments arranged by size:
2968 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5446
AvaI C'yCGr_G
Cuts at: 1870 1943 2771 1870
Size: 73 828 4545
Fragments arranged by size:
4545 828 73
BamHI G'GATC_C
Cuts at: 2232 2776 3143 2232
Size: 544 367 4535
Fragments arranged by size:
4535 544 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2767 4222 618
Size: 325 217 627 980 1455 1842
Fragments arranged by size:
1842 1455 980 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2108 2383 2632 2765 1391
Size: 717 275 249 133 4072
Fragments arranged by size:
4072 717 275 249 133
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4475
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3637 3671 1903
Size: 34 1700 34 3678
Fragments arranged by size:
3678 1700 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2210 3338 4548 2210
Size: 1128 1210 3108
Fragments arranged by size:
3108 1210 1128
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5446
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2186 2270 2627
Size: 107 122 71 631 605 514 84 357
Cuts at: 2627 4057 136
Size: 1430 1525
Fragments arranged by size:
1525 1430 631 605 514 357 122 107
84 71
BglII A'GATC_T
Cuts at: 5441 5441
Size: 5446
BlpI GC'TnA_GC
Cuts at: 2863 2863
Size: 5446
Bme1580I G_kGCm'C
Cuts at: 1790 2012 2043 2302 2383 2484 3507 4663 1790
Size: 222 31 259 81 101 1023 1156 2573
Fragments arranged by size:
2573 1156 1023 259 222 101 81 31
BmgBI CAC'GTC
Cuts at: 1318 2569 1318
Size: 1251 4195
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2434 3177 4135
Size: 416 53 83 399 24 1017 743 958
Cuts at: 4135 442
Size: 1753
Fragments arranged by size:
1753 1017 958 743 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5446
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2428 2616 4106 1239
Size: 1189 188 1490 2579
Fragments arranged by size:
2579 1490 1189 188
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3571 4436 4734 3571
Size: 865 298 4283
Fragments arranged by size:
4283 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4109 882
Size: 617 2610 2219
Fragments arranged by size:
2610 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3237 493
Size: 711 2033 2702
Fragments arranged by size:
2702 2033 711
BsaBI GATnn'nnATC
Cuts at: 2237 5440 2237
Size: 3203 2243
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2591 3253 3635 4270
Size: 53 83 186 936 1058 662 382 635
Cuts at: 4270 275
Size: 1451
Fragments arranged by size:
1451 1058 936 662 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3879 4277 4551 4698 5109 3879
Size: 398 274 147 411 4216
Fragments arranged by size:
4216 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2532 2562 1178
Size: 30 165 30 1129 30 4062
Fragments arranged by size:
4062 1129 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2112 1186
Size: 926 4520
BseYI C'CCAG_C
Cuts at: 2036 2134 4649 5145 2036
Size: 98 2515 496 2337
Fragments arranged by size:
2515 2337 496 98
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2190 2561 1163
Size: 573 454 371 4048
Fragments arranged by size:
4048 573 454 371
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2181 2896 3657 4685 1540
Size: 275 186 180 715 761 1028 2301
Fragments arranged by size:
2301 1028 761 715 275 186 180
BsiHKAI G_wGCw'C
Cuts at: 3507 3592 4663 3507
Size: 85 1071 4290
Fragments arranged by size:
4290 1071 85
BsmI GAATG_Cn'
Cuts at: 2957 3050 2957
Size: 93 5353
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2043 2108 2302 2383 2484 2632
Size: 399 222 31 65 194 81 101 148
Cuts at: 2632 2765 3507 3592 4663 1391
Size: 133 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 222 194 148 133
101 85 81 65 31
BspEI T'CCGG_A
Cuts at: 5109 5109
Size: 5446
BspHI T'CATG_A
Cuts at: 3335 5065 3335
Size: 1730 3716
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2093 2331 2801 844
Size: 1249 238 470 3489
Fragments arranged by size:
3489 1249 470 238
BsrBI CCG'CTC
Cuts at: 1394 1823 3333 1394
Size: 429 1510 3507
Fragments arranged by size:
3507 1510 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3941 4115 65
Size: 1183 2693 174 1396
Fragments arranged by size:
2693 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4090 4277 1700
Size: 110 2280 187 2869
Fragments arranged by size:
2869 2280 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5446
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5446
BssSI C'ACGA_G
Cuts at: 3506 4518 3506
Size: 1012 4434
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5446
BstBI TT'CG_AA
Cuts at: 4250 4250
Size: 5446
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5446
BstXI CCAn_nnnn'nTGG
Cuts at: 2036 2343 2036
Size: 307 5139
BstYI r'GATC_y
Cuts at: 1972 2232 2776 3143 3528 3545 4997 5083 5095
Size: 260 544 367 385 17 1452 86 12
Cuts at: 5095 5441 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 544 385 367 346 260 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2166 2319 2166
Size: 153 5293
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3955
Fragments arranged by size:
3955 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2062 487
Size: 683 56 836 3871
Fragments arranged by size:
3871 836 683 56
BtsI GCAGTG_nn'
Cuts at: 2962 3756 3776 2962
Size: 794 20 4632
Fragments arranged by size:
4632 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5446
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5411
DraI TTT'AAA
Cuts at: 2750 2750
Size: 5446
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4453 809
Size: 507 3137 1802
Fragments arranged by size:
3137 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2178 2893 3782
Size: 54 1425 50 275 18 348 715 889
Cuts at: 3782 5411 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 715 348 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2178 2893 1537
Size: 275 366 715 4090
Fragments arranged by size:
4090 715 366 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2439 3376 1353
Size: 1086 937 3423
Fragments arranged by size:
3423 1086 937
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4033 4407 4553 387
Size: 1064 138 2444 374 146 1280
Fragments arranged by size:
2444 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2428 2616 3509 4106 4893 5161 1239
Size: 393 796 188 893 597 787 268 1524
Fragments arranged by size:
1524 893 796 787 597 393 268 188
EcoNI CCTnn'n_nnAGG
Cuts at: 2127 2306 2318 2127
Size: 179 12 5255
Fragments arranged by size:
5255 179 12
EcoO109I rG'GnC_Cy
Cuts at: 2311 2328 2379 2311
Size: 17 51 5378
Fragments arranged by size:
5378 51 17
EcoRI G'AATT_C
Cuts at: 2755 3133 2755
Size: 378 5068
FspI TGC'GCA
Cuts at: 3952 3952
Size: 5446
HaeII r_GCGC'y
Cuts at: 1678 2151 4593 1678
Size: 473 2442 2531
Fragments arranged by size:
2531 2442 473
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2107 2139 2532 2564 4087
Size: 32 135 32 535 32 393 32 1523
Cuts at: 4087 4119 4161 4193 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1523 535 393 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2790 3036 669
Size: 988 281 852 246 3079
Fragments arranged by size:
3079 988 852 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5446
HpaI GTT'AAC
Cuts at: 3036 3036
Size: 5446
KpnI G_GTAC'C
Cuts at: 2771 2771
Size: 5446
MfeI C'AATT_G
Cuts at: 3045 3045
Size: 5446
MluI A'CGCG_T
Cuts at: 5322 5322
Size: 5446
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2393 4560 4744 1305
Size: 1088 2167 184 2007
Fragments arranged by size:
2167 2007 1088 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3624
Fragments arranged by size:
3624 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2691 2693 3404 3763 3922 518
Size: 648 1525 2 711 359 159 2042
Fragments arranged by size:
2042 1525 711 648 359 159 2
MspA1I CmG'CkG
Cuts at: 1606 2862 3539 4687 4932 1606
Size: 1256 677 1148 245 2120
Fragments arranged by size:
2120 1256 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3955
Fragments arranged by size:
3955 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5446
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5446
NotI GC'GGCC_GC
Cuts at: 2893 2893
Size: 5446
NspI r_CATG'y
Cuts at: 1132 1229 2804 4349 1132
Size: 97 1575 1545 2229
Fragments arranged by size:
2229 1575 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2267 1348
Size: 919 4527
PciI A'CATG_T
Cuts at: 4345 4345
Size: 5446
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2375 1108
Size: 1267 4179
PmeI GTTT'AAAC
Cuts at: 2750 2750
Size: 5446
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3515 3547 5028 5060 3515
Size: 32 1481 32 3901
Fragments arranged by size:
3901 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5446
PsiI TTA'TAA
Cuts at: 3016 3016
Size: 5446
PspOMI G'GGCC_C
Cuts at: 2379 2379
Size: 5446
PstI C_TGCA'G
Cuts at: 830 2798 830
Size: 1968 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5446
SalI G'TCGA_C
Cuts at: 1936 2788 1936
Size: 852 4594
SbfI CC_TGCA'GG
Cuts at: 2798 2798
Size: 5446
ScaI AGT'ACT
Cuts at: 3694 5406 3694
Size: 1712 3734
SfcI C'TryA_G
Cuts at: 826 1045 2794 3929 4610 4801 826
Size: 219 1749 1135 681 191 1471
Fragments arranged by size:
1749 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5446
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5446
SmaI CCC'GGG
Cuts at: 1872 2773 1872
Size: 901 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3550 4451 4713 820
Size: 197 926 1607 901 262 1553
Fragments arranged by size:
1607 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3237 493
Size: 2744 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5446
SphI G_CATG'C
Cuts at: 1132 2804 1132
Size: 1672 3774
SspI AAT'ATT
Cuts at: 5 52 3370 5
Size: 47 3318 2081
Fragments arranged by size:
3318 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5446
StyI C'CwwG_G
Cuts at: 513 1065 2004 2707 2885 513
Size: 552 939 703 178 3074
Fragments arranged by size:
3074 939 703 552 178
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2535 2681 3473 3490 3643 3828 4273
Size: 1255 955 146 792 17 153 185 445
Cuts at: 4273 325
Size: 1498
Fragments arranged by size:
1498 1255 955 792 445 185 153 146
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3692 5404 96
Size: 274 80 33 51 1443 1715 1712 138
Fragments arranged by size:
1715 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5406 5438 5406
Size: 32 5414
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5446
XbaI T'CTAG_A
Cuts at: 2782 2782
Size: 5446
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2534 2534
Size: 5446
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5446
XmaI C'CCGG_G
Cuts at: 1870 2771 1870
Size: 901 4545
XmnI GAAnn'nnTTC
Cuts at: 2656 3575 2656
Size: 919 4527
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2592 3254 276
Size: 53 83 186 936 1058 662 2468
Fragments arranged by size:
2468 1058 936 662 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI