Restriction map for
pFN21AE0592
(Circular) (Six-base) MAPSORT of: pFN21AE0592.seq Check: 3486 from: 1 to: 6133
pFN21AE0592 6133 bp
With 182 enzymes: SgfI *
October 20, 2009 10:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3943 278
Size: 53 83 186 936 2407 2468
Fragments arranged by size:
2468 2407 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3093 3476 1937
Size: 1156 383 4594
Fragments arranged by size:
4594 1156 383
Acc65I G'GTAC_C
Cuts at: 3454 3454
Size: 6133
AclI AA'CG_TT
Cuts at: 1563 4260 4633 1563
Size: 2697 373 3063
Fragments arranged by size:
3063 2697 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2154 2923 3206 4196 5580 5848 1632
Size: 522 769 283 990 1384 268 1917
Fragments arranged by size:
1917 1384 990 769 522 283 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5936
AflIII A'CryG_T
Cuts at: 1203 2982 5032 6009 1203
Size: 1779 2050 977 1327
Fragments arranged by size:
2050 1779 1327 977
AgeI A'CCGG_T
Cuts at: 3246 4964 3246
Size: 1718 4415
AhdI GACnn_n'nnGTC
Cuts at: 4862 4862
Size: 6133
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6133
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6133
AlwNI CAG_nnn'CTG
Cuts at: 3876 5448 3876
Size: 1572 4561
ApaI G_GGCC'C
Cuts at: 2999 3295 3424 2999
Size: 296 129 5708
Fragments arranged by size:
5708 296 129
ApaLI G'TGCA_C
Cuts at: 4190 5346 4190
Size: 1156 4977
ApoI r'AATT_y
Cuts at: 1493 2015 2058 2442 2627 3442 3668 3820 5929
Size: 522 43 384 185 815 226 152 2109
Cuts at: 5929 1493
Size: 1697
Fragments arranged by size:
2109 1697 815 522 384 226 185 152
43
AseI AT'TA_AT
Cuts at: 160 1032 4687 160
Size: 872 3655 1606
Fragments arranged by size:
3655 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6133
AvaI C'yCGr_G
Cuts at: 1870 1943 3458 1870
Size: 73 1515 4545
Fragments arranged by size:
4545 1515 73
AvrII C'CTAG_G
Cuts at: 3316 3316
Size: 6133
BamHI G'GATC_C
Cuts at: 3342 3463 3830 3342
Size: 121 367 5645
Fragments arranged by size:
5645 367 121
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2291 2969 3320 3454 4909
Size: 325 217 627 504 678 351 134 1455
Cuts at: 4909 618
Size: 1842
Fragments arranged by size:
1842 1455 678 627 504 351 325 217
134
BanII G_rGCy'C
Cuts at: 1391 2335 2350 2692 2999 3116 3295 3424 3452
Size: 944 15 342 307 117 179 129 28
Cuts at: 3452 1391
Size: 4072
Fragments arranged by size:
4072 944 342 307 179 129 117 28
15
BbeI G_GCGC'C
Cuts at: 2295 3324 2295
Size: 1029 5104
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2160 2979 3078 928
Size: 971 261 819 99 3983
Fragments arranged by size:
3983 971 819 261 99
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4324 4358 1903
Size: 34 2387 34 3678
Fragments arranged by size:
3678 2387 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4025 5235 4025
Size: 1210 4923
BclI T'GATC_A
Cuts at: 1548 2367 1548
Size: 819 5314
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2967 4744 136
Size: 107 122 71 631 605 1295 1777 1525
Fragments arranged by size:
1777 1525 1295 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3381 6128 3381
Size: 2747 3386
BlpI GC'TnA_GC
Cuts at: 3550 3550
Size: 6133
Bme1580I G_kGCm'C
Cuts at: 1790 2972 2999 3295 3424 4194 5350 1790
Size: 1182 27 296 129 770 1156 2573
Fragments arranged by size:
2573 1182 1156 770 296 129 27
BmgBI CAC'GTC
Cuts at: 1318 2262 2985 1318
Size: 944 723 4466
Fragments arranged by size:
4466 944 723
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3864 4822 442
Size: 416 53 83 399 24 2447 958 1753
Fragments arranged by size:
2447 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6133
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3030 4793 1239
Size: 1791 1763 2579
Fragments arranged by size:
2579 1791 1763
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4258 5123 5421 4258
Size: 865 298 4970
Fragments arranged by size:
4970 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3154 3215 4796 882
Size: 617 1655 61 1581 2219
Fragments arranged by size:
2219 1655 1581 617 61
BsaAI yAC'GTr
Cuts at: 493 1204 3005 3924 493
Size: 711 1801 919 2702
Fragments arranged by size:
2702 1801 919 711
BsaBI GATnn'nnATC
Cuts at: 6127 6127
Size: 6133
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2292 3321 3940 4322
Size: 53 83 186 936 759 1029 619 382
Cuts at: 4322 4957 275
Size: 635 1451
Fragments arranged by size:
1451 1029 936 759 635 619 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3246 4566 4964 5238 5385 5796 3246
Size: 1320 398 274 147 411 3583
Fragments arranged by size:
3583 1320 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5908
Fragments arranged by size:
5908 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6133
BseYI C'CCAG_C
Cuts at: 2965 2998 3109 5336 5832 2965
Size: 33 111 2227 496 3266
Fragments arranged by size:
3266 2227 496 111 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5560
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3583 4344 5372 1540
Size: 275 186 1582 761 1028 2301
Fragments arranged by size:
2301 1582 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2335 4194 4279 5350 2335
Size: 1859 85 1071 3118
Fragments arranged by size:
3118 1859 1071 85
BsmI GAATG_Cn'
Cuts at: 2588 3122 3644 3737 2588
Size: 534 522 93 4984
Fragments arranged by size:
4984 534 522 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2335 2350 2692 2972 2999 3116 3295
Size: 399 545 15 342 280 27 117 179
Cuts at: 3295 3424 3452 4194 4279 5350 1391
Size: 129 28 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 545 399 342 280 179
129 117 85 28 27 15
BspEI T'CCGG_A
Cuts at: 5796 5796
Size: 6133
BspHI T'CATG_A
Cuts at: 2730 4022 5752 2730
Size: 1292 1730 3111
Fragments arranged by size:
3111 1730 1292
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2805 3488 844
Size: 1961 683 3489
Fragments arranged by size:
3489 1961 683
BsrBI CCG'CTC
Cuts at: 1394 1823 4020 1394
Size: 429 2197 3507
Fragments arranged by size:
3507 2197 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2550 4628 4802 65
Size: 1183 1302 2078 174 1396
Fragments arranged by size:
2078 1396 1302 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3246 4777 4964 1700
Size: 110 1436 1531 187 2869
Fragments arranged by size:
2869 1531 1436 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6133
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6133
BssSI C'ACGA_G
Cuts at: 4193 5205 4193
Size: 1012 5121
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6133
BstBI TT'CG_AA
Cuts at: 4937 4937
Size: 6133
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6133
BstYI r'GATC_y
Cuts at: 1972 2244 3342 3381 3463 3830 4215 4232 5684
Size: 272 1098 39 82 367 385 17 1452
Cuts at: 5684 5770 5782 6128 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1098 385 367 346 272 86
82 39 17 12
BstZ17I GTA'TAC
Cuts at: 3094 3094
Size: 6133
BtgI C'CryG_G
Cuts at: 513 1065 2004 2821 3228 3264 513
Size: 552 939 817 407 36 3382
Fragments arranged by size:
3382 939 817 552 407 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2629 2725 487
Size: 683 56 1403 96 3895
Fragments arranged by size:
3895 1403 683 96 56
BtsI GCAGTG_nn'
Cuts at: 2769 3649 4443 4463 2769
Size: 880 794 20 4439
Fragments arranged by size:
4439 880 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6133
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2734 2769 529
Size: 35 2170 35 3893
Fragments arranged by size:
3893 2170 35 35
DraI TTT'AAA
Cuts at: 3437 3437
Size: 6133
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5140 809
Size: 507 3824 1802
Fragments arranged by size:
3824 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2380 2614 2664
Size: 54 1425 50 275 18 550 234 50
Cuts at: 2664 2824 3580 4469 6098 8
Size: 160 756 889 1629 43
Fragments arranged by size:
1629 1425 889 756 550 275 234 160
54 50 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3580 1537
Size: 275 1768 4090
Fragments arranged by size:
4090 1768 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2342 2552 2858 3434 4063 1353
Size: 989 210 306 576 629 3423
Fragments arranged by size:
3423 989 629 576 306 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4720 5094 5240 387
Size: 1064 138 3131 374 146 1280
Fragments arranged by size:
3131 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2333 2333
Size: 6133
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2154 2923 3030 3206 4196 4793 5580
Size: 393 522 769 107 176 990 597 787
Cuts at: 5580 5848 1239
Size: 268 1524
Fragments arranged by size:
1524 990 787 769 597 522 393 268
176 107
EcoNI CCTnn'n_nnAGG
Cuts at: 2696 2696
Size: 6133
EcoO109I rG'GnC_Cy
Cuts at: 2419 2995 3291 3292 3420 3421 2419
Size: 576 296 1 128 1 5131
Fragments arranged by size:
5131 576 296 128 1 1
EcoRI G'AATT_C
Cuts at: 3442 3820 3442
Size: 378 5755
FspI TGC'GCA
Cuts at: 4639 4639
Size: 6133
HaeII r_GCGC'y
Cuts at: 1678 2295 3324 5280 1678
Size: 617 1029 1956 2531
Fragments arranged by size:
2531 1956 1029 617
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4774 4806 4848 4880 1373
Size: 32 135 32 3202 32 42 32 2626
Fragments arranged by size:
3202 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2608 3477 3723 669
Size: 988 281 670 869 246 3079
Fragments arranged by size:
3079 988 869 670 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6133
HpaI GTT'AAC
Cuts at: 3723 3723
Size: 6133
KasI G'GCGC_C
Cuts at: 2291 3320 2291
Size: 1029 5104
KpnI G_GTAC'C
Cuts at: 3458 3458
Size: 6133
MfeI C'AATT_G
Cuts at: 3732 3732
Size: 6133
MluI A'CGCG_T
Cuts at: 6009 6009
Size: 6133
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2889 2925 2958 5247 5431 1305
Size: 1584 36 33 2289 184 2007
Fragments arranged by size:
2289 2007 1584 184 36 33
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2666 2826 10
Size: 54 1425 343 834 160 3317
Fragments arranged by size:
3317 1425 834 343 160 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2231 4091 4450 4609 518
Size: 648 1065 1860 359 159 2042
Fragments arranged by size:
2042 1860 1065 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2180 3184 3266 3549 4226 5374 5619 1606
Size: 574 1004 82 283 677 1148 245 2120
Fragments arranged by size:
2120 1148 1004 677 574 283 245 82
NarI GG'CG_CC
Cuts at: 2292 3321 2292
Size: 1029 5104
NcoI C'CATG_G
Cuts at: 513 1065 2004 3228 513
Size: 552 939 1224 3418
Fragments arranged by size:
3418 1224 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6133
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6133
NotI GC'GGCC_GC
Cuts at: 3580 3580
Size: 6133
NspI r_CATG'y
Cuts at: 1132 1229 3491 5036 1132
Size: 97 2262 1545 2229
Fragments arranged by size:
2262 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6133
PciI A'CATG_T
Cuts at: 5032 5032
Size: 6133
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2580 2662 3020 3138 1108
Size: 1472 82 358 118 4103
Fragments arranged by size:
4103 1472 358 118 82
PmeI GTTT'AAAC
Cuts at: 3437 3437
Size: 6133
PmlI CAC'GTG
Cuts at: 3005 3005
Size: 6133
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4202 4234 5715 5747 4202
Size: 32 1481 32 4588
Fragments arranged by size:
4588 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2419 2419
Size: 6133
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6133
PsiI TTA'TAA
Cuts at: 2273 3703 2273
Size: 1430 4703
PspOMI G'GGCC_C
Cuts at: 2995 3291 3420 2995
Size: 296 129 5708
Fragments arranged by size:
5708 296 129
PstI C_TGCA'G
Cuts at: 830 2886 3485 830
Size: 2056 599 3478
Fragments arranged by size:
3478 2056 599
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6133
PvuII CAG'CTG
Cuts at: 2180 3184 2180
Size: 1004 5129
SacI G_AGCT'C
Cuts at: 2335 2335
Size: 6133
SacII CC_GC'GG
Cuts at: 3267 3267
Size: 6133
SalI G'TCGA_C
Cuts at: 1936 3475 1936
Size: 1539 4594
SanDI GG'GwC_CC
Cuts at: 2419 2419
Size: 6133
SapI GCTCTTCn'nnn_
Cuts at: 2858 3434 2858
Size: 576 5557
SbfI CC_TGCA'GG
Cuts at: 3485 3485
Size: 6133
ScaI AGT'ACT
Cuts at: 3374 4381 6093 3374
Size: 1007 1712 3414
Fragments arranged by size:
3414 1712 1007
SexAI A'CCwGG_T
Cuts at: 2972 2972
Size: 6133
SfcI C'TryA_G
Cuts at: 826 1045 2882 3481 4616 5297 5488 826
Size: 219 1837 599 1135 681 191 1471
Fragments arranged by size:
1837 1471 1135 681 599 219 191
SfoI GGC'GCC
Cuts at: 2293 3322 2293
Size: 1029 5104
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6133
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6133
SmaI CCC'GGG
Cuts at: 1872 3460 1872
Size: 1588 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4237 5138 5400 820
Size: 197 926 2294 901 262 1553
Fragments arranged by size:
2294 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3924 493
Size: 3431 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6133
SphI G_CATG'C
Cuts at: 1132 3491 1132
Size: 2359 3774
SspI AAT'ATT
Cuts at: 5 52 4057 5
Size: 47 4005 2081
Fragments arranged by size:
4005 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6133
StyI C'CwwG_G
Cuts at: 513 1065 2004 2527 3228 3316 3572 513
Size: 552 939 523 701 88 256 3074
Fragments arranged by size:
3074 939 701 552 523 256 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2343 2423 3289 4160 4177 4330 4515
Size: 1255 763 80 866 871 17 153 185
Cuts at: 4515 4960 325
Size: 445 1498
Fragments arranged by size:
1498 1255 871 866 763 445 185 153
80 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2829 3372 4379
Size: 274 80 33 51 1443 852 543 1007
Cuts at: 4379 6091 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1007 852 543 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6093 6125 6093
Size: 32 6101
Tth111I GACn'n_nGTC
Cuts at: 1316 2681 3077 1316
Size: 1365 396 4372
Fragments arranged by size:
4372 1365 396
XbaI T'CTAG_A
Cuts at: 3469 3469
Size: 6133
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2357 2357
Size: 6133
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6133
XmaI C'CCGG_G
Cuts at: 1870 3458 1870
Size: 1588 4545
XmnI GAAnn'nnTTC
Cuts at: 2588 4262 2588
Size: 1674 4459
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3941 276
Size: 53 83 186 936 2407 2468
Fragments arranged by size:
2468 2407 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PspXI PsrI RsrII SfiI SrfI SwaI