Restriction map for
pFN21AE0591
(Circular) (Six-base) MAPSORT of: pFN21AE0591.seq Check: 1094 from: 1 to: 5941
pFN21AE0591 5941 bp
With 182 enzymes: SgfI *
August 16, 2010 14:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2520 2520
Size: 5941
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3751 278
Size: 53 83 186 936 2215 2468
Fragments arranged by size:
2468 2215 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3284 1937
Size: 1347 4594
Acc65I G'GTAC_C
Cuts at: 3262 3262
Size: 5941
AclI AA'CG_TT
Cuts at: 1563 4068 4441 1563
Size: 2505 373 3063
Fragments arranged by size:
3063 2505 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2383 2483 2729 2802 3167 3188 4004 5388
Size: 751 100 246 73 365 21 816 1384
Cuts at: 5388 5656 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 816 751 365 268 246 100
73 21
AfeI AGC'GCT
Cuts at: 3194 3194
Size: 5941
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5744
AflIII A'CryG_T
Cuts at: 1203 2067 2696 2698 4840 5817 1203
Size: 864 629 2 2142 977 1327
Fragments arranged by size:
2142 1327 977 864 629 2
AgeI A'CCGG_T
Cuts at: 4772 4772
Size: 5941
AhdI GACnn_n'nnGTC
Cuts at: 4670 4670
Size: 5941
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5941
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2654 1184
Size: 1470 4471
AlwNI CAG_nnn'CTG
Cuts at: 3227 3684 5256 3227
Size: 457 1572 3912
Fragments arranged by size:
3912 1572 457
ApaI G_GGCC'C
Cuts at: 2154 2291 2154
Size: 137 5804
ApaLI G'TGCA_C
Cuts at: 3998 5154 3998
Size: 1156 4785
ApoI r'AATT_y
Cuts at: 1493 2666 3250 3476 3628 5737 1493
Size: 1173 584 226 152 2109 1697
Fragments arranged by size:
2109 1697 1173 584 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4495 160
Size: 872 3463 1606
Fragments arranged by size:
3463 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5941
AvaI C'yCGr_G
Cuts at: 1870 1943 2154 2284 2296 3266 1870
Size: 73 211 130 12 970 4545
Fragments arranged by size:
4545 970 211 130 73 12
BamHI G'GATC_C
Cuts at: 3271 3638 3271
Size: 367 5574
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2201 2228 3262 4717 618
Size: 325 217 627 414 27 1034 1455 1842
Fragments arranged by size:
1842 1455 1034 627 414 325 217 27
BanII G_rGCy'C
Cuts at: 1391 2154 2291 3219 3260 1391
Size: 763 137 928 41 4072
Fragments arranged by size:
4072 928 763 137 41
BbeI G_GCGC'C
Cuts at: 2232 2232
Size: 5941
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2625 2790 928
Size: 971 726 165 4079
Fragments arranged by size:
4079 971 726 165
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2360 2394 4132 4166 1903
Size: 34 423 34 1738 34 3678
Fragments arranged by size:
3678 1738 423 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3833 5043 3833
Size: 1210 4731
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5941
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2531 2882 4552
Size: 107 122 71 631 605 859 351 1670
Cuts at: 4552 136
Size: 1525
Fragments arranged by size:
1670 1525 859 631 605 351 122 107
71
BglII A'GATC_T
Cuts at: 2100 5936 2100
Size: 3836 2105
BlpI GC'TnA_GC
Cuts at: 3233 3358 3233
Size: 125 5816
Bme1580I G_kGCm'C
Cuts at: 1790 2075 2154 2291 2390 2706 4002 5158 1790
Size: 285 79 137 99 316 1296 1156 2573
Fragments arranged by size:
2573 1296 1156 316 285 137 99 79
BmgBI CAC'GTC
Cuts at: 1318 2068 1318
Size: 750 5191
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3672 4630 442
Size: 416 53 83 399 24 2255 958 1753
Fragments arranged by size:
2255 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5941
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2263 2774 2816 3077 4601 1239
Size: 1024 511 42 261 1524 2579
Fragments arranged by size:
2579 1524 1024 511 261 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2810 4066 4931 5229 2810
Size: 1256 865 298 3522
Fragments arranged by size:
3522 1256 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2582 4604 882
Size: 617 1083 2022 2219
Fragments arranged by size:
2219 2022 1083 617
BsaAI yAC'GTr
Cuts at: 493 1204 2699 2920 3732 493
Size: 711 1495 221 812 2702
Fragments arranged by size:
2702 1495 812 711 221
BsaBI GATnn'nnATC
Cuts at: 5935 5935
Size: 5941
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2145 2175 2229 2349
Size: 53 83 186 936 612 30 54 120
Cuts at: 2349 2895 3748 4130 4765 275
Size: 546 853 382 635 1451
Fragments arranged by size:
1451 936 853 635 612 546 382 186
120 83 54 53 30
BsaWI w'CCGG_w
Cuts at: 4374 4772 5046 5193 5604 4374
Size: 398 274 147 411 4711
Fragments arranged by size:
4711 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5716
Fragments arranged by size:
5716 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2813 3023 1186
Size: 1627 210 4104
Fragments arranged by size:
4104 1627 210
BseYI C'CCAG_C
Cuts at: 2291 2303 5144 5640 2291
Size: 12 2841 496 2592
Fragments arranged by size:
2841 2592 496 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2148 2359 2422 2492 1163
Size: 573 412 211 63 70 4612
Fragments arranged by size:
4612 573 412 211 70 63
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2209 2318 3391 4152 5180 1540
Size: 275 186 208 109 1073 761 1028 2301
Fragments arranged by size:
2301 1073 1028 761 275 208 186 109
BsiHKAI G_wGCw'C
Cuts at: 3219 3234 4002 4087 5158 3219
Size: 15 768 85 1071 4002
Fragments arranged by size:
4002 1071 768 85 15
BsmI GAATG_Cn'
Cuts at: 3452 3545 3452
Size: 93 5848
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2075 2154 2291 2390 2706 3219 3234
Size: 399 285 79 137 99 316 513 15
Cuts at: 3234 3260 4002 4087 5158 1391
Size: 26 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 513 399 316 285 137
99 85 79 26 15
BspEI T'CCGG_A
Cuts at: 5604 5604
Size: 5941
BspHI T'CATG_A
Cuts at: 3830 5560 3830
Size: 1730 4211
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2520 3296 844
Size: 1676 776 3489
Fragments arranged by size:
3489 1676 776
BsrBI CCG'CTC
Cuts at: 1394 1823 2803 3828 1394
Size: 429 980 1025 3507
Fragments arranged by size:
3507 1025 980 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4436 4610 65
Size: 1183 3188 174 1396
Fragments arranged by size:
3188 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2198 2204 2767 4585 4772 1700
Size: 110 388 6 563 1818 187 2869
Fragments arranged by size:
2869 1818 563 388 187 110 6
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5941
BssHII G'CGCG_C
Cuts at: 1925 2183 1925
Size: 258 5683
BssSI C'ACGA_G
Cuts at: 4001 5013 4001
Size: 1012 4929
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2515 2851 794
Size: 1721 336 3884
Fragments arranged by size:
3884 1721 336
BstBI TT'CG_AA
Cuts at: 4745 4745
Size: 5941
BstEII G'GTnAC_C
Cuts at: 1184 3061 1184
Size: 1877 4064
BstYI r'GATC_y
Cuts at: 1972 2100 2643 3271 3638 4023 4040 5492 5578
Size: 128 543 628 367 385 17 1452 86
Cuts at: 5578 5590 5936 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 628 543 385 367 346 128
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2933 2933
Size: 5941
BtgI C'CryG_G
Cuts at: 513 1065 2126 2889 513
Size: 552 1061 763 3565
Fragments arranged by size:
3565 1061 763 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5202
Fragments arranged by size:
5202 683 56
BtsI GCAGTG_nn'
Cuts at: 2376 2434 3457 4251 4271 2376
Size: 58 1023 794 20 4046
Fragments arranged by size:
4046 1023 794 58 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5941
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5906
DraI TTT'AAA
Cuts at: 3245 3245
Size: 5941
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2498 4948 809
Size: 507 1182 2450 1802
Fragments arranged by size:
2450 1802 1182 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2058 2196 2206
Size: 54 1425 50 275 18 228 138 10
Cuts at: 2206 2315 2883 3042 3388 4277 5906 8
Size: 109 568 159 346 889 1629 43
Fragments arranged by size:
1629 1425 889 568 346 275 228 159
138 109 54 50 43 18 10
EagI C'GGCC_G
Cuts at: 1537 1812 2206 2315 3388 1537
Size: 275 394 109 1073 4090
Fragments arranged by size:
4090 1073 394 275 109
EarI CTCTTCn'nnn_
Cuts at: 1353 2267 3871 1353
Size: 914 1604 3423
Fragments arranged by size:
3423 1604 914
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4528 4902 5048 387
Size: 1064 138 2939 374 146 1280
Fragments arranged by size:
2939 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3217 3217
Size: 5941
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2263 2383 2483 2729 2774 2802 2816
Size: 393 631 120 100 246 45 28 14
Cuts at: 2816 3077 3167 3188 4004 4601 5388 5656 1239
Size: 261 90 21 816 597 787 268 1524
Fragments arranged by size:
1524 816 787 631 597 393 268 261
246 120 100 90 45 28 21 14
EcoO109I rG'GnC_Cy
Cuts at: 2131 2151 2288 2300 2458 3037 2131
Size: 20 137 12 158 579 5035
Fragments arranged by size:
5035 579 158 137 20 12
EcoRI G'AATT_C
Cuts at: 3250 3628 3250
Size: 378 5563
FspI TGC'GCA
Cuts at: 4447 4447
Size: 5941
HaeII r_GCGC'y
Cuts at: 1678 2232 3145 3196 5088 1678
Size: 554 913 51 1892 2531
Fragments arranged by size:
2531 1892 913 554 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2939 2971 4582 4614 4656
Size: 32 135 32 1367 32 1611 32 42
Cuts at: 4656 4688 1373
Size: 32 2626
Fragments arranged by size:
2626 1611 1367 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3285 3531 669
Size: 988 281 1347 246 3079
Fragments arranged by size:
3079 1347 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5941
HpaI GTT'AAC
Cuts at: 3531 3531
Size: 5941
KasI G'GCGC_C
Cuts at: 2228 2228
Size: 5941
KpnI G_GTAC'C
Cuts at: 3266 3266
Size: 5941
MfeI C'AATT_G
Cuts at: 3540 3540
Size: 5941
MluI A'CGCG_T
Cuts at: 5817 5817
Size: 5941
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2130 2247 2346 3117 5055 5239 1305
Size: 825 117 99 771 1938 184 2007
Fragments arranged by size:
2007 1938 825 771 184 117 99
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2060 3044 10
Size: 54 1425 343 228 984 2907
Fragments arranged by size:
2907 1425 984 343 228 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2066 3899 4258 4417 518
Size: 648 900 1833 359 159 2042
Fragments arranged by size:
2042 1833 900 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2128 2162 2764 2972 3357 4034 5182 5427
Size: 522 34 602 208 385 677 1148 245
Cuts at: 5427 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 602 522 385 245 208
34
NaeI GCC'GGC
Cuts at: 2206 2206
Size: 5941
NarI GG'CG_CC
Cuts at: 2229 2229
Size: 5941
NcoI C'CATG_G
Cuts at: 513 1065 2889 513
Size: 552 1824 3565
Fragments arranged by size:
3565 1824 552
NdeI CA'TA_TG
Cuts at: 387 2055 387
Size: 1668 4273
NgoMIV G'CCGG_C
Cuts at: 2204 2204
Size: 5941
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5941
NotI GC'GGCC_GC
Cuts at: 2315 3388 2315
Size: 1073 4868
NspI r_CATG'y
Cuts at: 1132 1229 3299 4844 1132
Size: 97 2070 1545 2229
Fragments arranged by size:
2229 2070 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2383 3221 1348
Size: 1035 838 4068
Fragments arranged by size:
4068 1035 838
PciI A'CATG_T
Cuts at: 4840 4840
Size: 5941
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5941
PfoI T'CCnGG_A
Cuts at: 2046 2046
Size: 5941
PmeI GTTT'AAAC
Cuts at: 3245 3245
Size: 5941
PmlI CAC'GTG
Cuts at: 2699 2699
Size: 5941
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2539 2571 4010 4042 5523 5555 2539
Size: 32 1439 32 1481 32 2925
Fragments arranged by size:
2925 1481 1439 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2300 3037 2300
Size: 737 5204
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5941
PsiI TTA'TAA
Cuts at: 3511 3511
Size: 5941
PspOMI G'GGCC_C
Cuts at: 2150 2287 2150
Size: 137 5804
PstI C_TGCA'G
Cuts at: 830 3293 830
Size: 2463 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5941
PvuII CAG'CTG
Cuts at: 2764 2764
Size: 5941
SacI G_AGCT'C
Cuts at: 3219 3219
Size: 5941
SacII CC_GC'GG
Cuts at: 2129 2129
Size: 5941
SalI G'TCGA_C
Cuts at: 1936 3283 1936
Size: 1347 4594
SanDI GG'GwC_CC
Cuts at: 2300 2300
Size: 5941
SapI GCTCTTCn'nnn_
Cuts at: 2267 2267
Size: 5941
SbfI CC_TGCA'GG
Cuts at: 3293 3293
Size: 5941
ScaI AGT'ACT
Cuts at: 4189 5901 4189
Size: 1712 4229
SfcI C'TryA_G
Cuts at: 826 1045 3289 4424 5105 5296 826
Size: 219 2244 1135 681 191 1471
Fragments arranged by size:
2244 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2230 2230
Size: 5941
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5941
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5941
SmaI CCC'GGG
Cuts at: 1872 2286 2298 3268 1872
Size: 414 12 970 4545
Fragments arranged by size:
4545 970 414 12
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2825 4045 4946 5208 820
Size: 197 926 882 1220 901 262 1553
Fragments arranged by size:
1553 1220 926 901 882 262 197
SnaBI TAC'GTA
Cuts at: 493 3732 493
Size: 3239 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5941
SphI G_CATG'C
Cuts at: 1132 3299 1132
Size: 2167 3774
SrfI GCCC'GGGC
Cuts at: 2286 2286
Size: 5941
SspI AAT'ATT
Cuts at: 5 52 3865 5
Size: 47 3813 2081
Fragments arranged by size:
3813 2081 47
StuI AGG'CCT
Cuts at: 1517 2850 1517
Size: 1333 4608
StyI C'CwwG_G
Cuts at: 513 1065 2448 2454 2889 3132 3380 513
Size: 552 1383 6 435 243 248 3074
Fragments arranged by size:
3074 1383 552 435 248 243 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3968 3985 4138 4323 4768 325
Size: 1255 2388 17 153 185 445 1498
Fragments arranged by size:
2388 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2829 4187 5899
Size: 274 80 33 51 1443 852 1358 1712
Cuts at: 5899 96
Size: 138
Fragments arranged by size:
1712 1443 1358 852 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5901 5933 5901
Size: 32 5909
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5941
XbaI T'CTAG_A
Cuts at: 3277 3277
Size: 5941
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3052 3052
Size: 5941
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5941
XmaI C'CCGG_G
Cuts at: 1870 2284 2296 3266 1870
Size: 414 12 970 4545
Fragments arranged by size:
4545 970 414 12
XmnI GAAnn'nnTTC
Cuts at: 4070 4070
Size: 5941
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3749 276
Size: 53 83 186 936 2215 2468
Fragments arranged by size:
2468 2215 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FalI
FseI FspAI NruI NsiI PacI PspXI PsrI RsrII
SexAI SfiI SwaI