Restriction map for
pFN21AE0585
(Circular) (Six-base) MAPSORT of: pFN21AE0585.seq Check: 3229 from: 1 to: 5611
pFN21AE0585 5611 bp
With 182 enzymes: SgfI *
October 20, 2009 10:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2859 3421 278
Size: 53 83 186 936 1323 562 2468
Fragments arranged by size:
2468 1323 936 562 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2677 2954 1937
Size: 740 277 4594
Fragments arranged by size:
4594 740 277
Acc65I G'GTAC_C
Cuts at: 2932 2932
Size: 5611
AclI AA'CG_TT
Cuts at: 1563 3738 4111 1563
Size: 2175 373 3063
Fragments arranged by size:
3063 2175 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2766 3674 5058 5326 1632
Size: 1134 908 1384 268 1917
Fragments arranged by size:
1917 1384 1134 908 268
AflII C'TTAA_G
Cuts at: 820 1017 2261 820
Size: 197 1244 4170
Fragments arranged by size:
4170 1244 197
AflIII A'CryG_T
Cuts at: 1203 2610 4510 5487 1203
Size: 1407 1900 977 1327
Fragments arranged by size:
1900 1407 1327 977
AgeI A'CCGG_T
Cuts at: 4442 4442
Size: 5611
AhdI GACnn_n'nnGTC
Cuts at: 2662 2855 4340 2662
Size: 193 1485 3933
Fragments arranged by size:
3933 1485 193
AleI CACnn'nnGTG
Cuts at: 1166 2522 1166
Size: 1356 4255
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5611
AlwNI CAG_nnn'CTG
Cuts at: 2351 2519 3354 4926 2351
Size: 168 835 1572 3036
Fragments arranged by size:
3036 1572 835 168
ApaLI G'TGCA_C
Cuts at: 3668 4824 3668
Size: 1156 4455
ApoI r'AATT_y
Cuts at: 1493 2728 2920 3146 3298 5407 1493
Size: 1235 192 226 152 2109 1697
Fragments arranged by size:
2109 1697 1235 226 192 152
AseI AT'TA_AT
Cuts at: 160 1032 4165 160
Size: 872 3133 1606
Fragments arranged by size:
3133 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5611
AvaI C'yCGr_G
Cuts at: 1870 1943 2936 1870
Size: 73 993 4545
Fragments arranged by size:
4545 993 73
BamHI G'GATC_C
Cuts at: 2941 3308 2941
Size: 367 5244
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2932 4387 618
Size: 325 217 627 1145 1455 1842
Fragments arranged by size:
1842 1455 1145 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2930 1391
Size: 1539 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4640
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3802 3836 1903
Size: 34 1865 34 3678
Fragments arranged by size:
3678 1865 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2633 3503 4713 2633
Size: 870 1210 3531
Fragments arranged by size:
3531 1210 870
BclI T'GATC_A
Cuts at: 1548 2370 1548
Size: 822 4789
BfrBI ATG'CAT
Cuts at: 2300 2461 2479 2890 2894 2300
Size: 161 18 411 4 5017
Fragments arranged by size:
5017 411 161 18 4
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4222 136
Size: 107 122 71 631 605 2550 1525
Fragments arranged by size:
2550 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2254 2539 5606 2254
Size: 285 3067 2259
Fragments arranged by size:
3067 2259 285
BlpI GC'TnA_GC
Cuts at: 3028 3028
Size: 5611
Bme1580I G_kGCm'C
Cuts at: 1790 3672 4828 1790
Size: 1882 1156 2573
Fragments arranged by size:
2573 1882 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5611
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2529 3342 4300
Size: 416 53 83 399 24 1112 813 958
Cuts at: 4300 442
Size: 1753
Fragments arranged by size:
1753 1112 958 813 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5611
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2601 4271 1239
Size: 1362 1670 2579
Fragments arranged by size:
2579 1670 1362
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3736 4601 4899 3736
Size: 865 298 4448
Fragments arranged by size:
4448 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2670 4274 882
Size: 617 1171 1604 2219
Fragments arranged by size:
2219 1604 1171 617
BsaAI yAC'GTr
Cuts at: 493 1204 2231 3402 493
Size: 711 1027 1171 2702
Fragments arranged by size:
2702 1171 1027 711
BsaBI GATnn'nnATC
Cuts at: 5605 5605
Size: 5611
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2856 3418 3800 4435
Size: 53 83 186 936 1323 562 382 635
Cuts at: 4435 275
Size: 1451
Fragments arranged by size:
1451 1323 936 635 562 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2074 4044 4442 4716 4863 5274 2074
Size: 1970 398 274 147 411 2411
Fragments arranged by size:
2411 1970 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2166 2196 1178
Size: 30 165 30 763 30 4593
Fragments arranged by size:
4593 763 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5611
BseYI C'CCAG_C
Cuts at: 4814 5310 4814
Size: 496 5115
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2219 2565 1163
Size: 573 483 346 4209
Fragments arranged by size:
4209 573 483 346
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2075 3061 3822 4850 1540
Size: 275 186 74 986 761 1028 2301
Fragments arranged by size:
2301 1028 986 761 275 186 74
BsiHKAI G_wGCw'C
Cuts at: 3672 3757 4828 3672
Size: 85 1071 4455
Fragments arranged by size:
4455 1071 85
BsmI GAATG_Cn'
Cuts at: 2031 2461 3122 3215 2031
Size: 430 661 93 4427
Fragments arranged by size:
4427 661 430 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2930 3672 3757 4828 1391
Size: 399 1140 742 85 1071 2174
Fragments arranged by size:
2174 1140 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5274 5274
Size: 5611
BspHI T'CATG_A
Cuts at: 3500 5230 3500
Size: 1730 3881
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2966 844
Size: 2122 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3498 1394
Size: 429 1675 3507
Fragments arranged by size:
3507 1675 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4106 4280 65
Size: 1183 2858 174 1396
Fragments arranged by size:
2858 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2553 4255 4442 1700
Size: 110 743 1702 187 2869
Fragments arranged by size:
2869 1702 743 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5611
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5611
BssSI C'ACGA_G
Cuts at: 3671 4683 3671
Size: 1012 4599
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2900 794
Size: 2106 3505
BstBI TT'CG_AA
Cuts at: 2107 4415 2107
Size: 2308 3303
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5611
BstXI CCAn_nnnn'nTGG
Cuts at: 2524 2524
Size: 5611
BstYI r'GATC_y
Cuts at: 1972 2254 2539 2836 2941 3308 3693 3710 5162
Size: 282 285 297 105 367 385 17 1452
Cuts at: 5162 5248 5260 5606 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 297 285 282
105 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4120
Fragments arranged by size:
4120 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4872
Fragments arranged by size:
4872 683 56
BtsI GCAGTG_nn'
Cuts at: 2449 2898 3127 3921 3941 2449
Size: 449 229 794 20 4119
Fragments arranged by size:
4119 794 449 229 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5611
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5576
DraI TTT'AAA
Cuts at: 2190 2915 2190
Size: 725 4886
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4618 809
Size: 507 3302 1802
Fragments arranged by size:
3302 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2551 3058 3947
Size: 54 1425 50 275 18 721 507 889
Cuts at: 3947 5576 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 721 507 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3058 1537
Size: 275 1246 4090
Fragments arranged by size:
4090 1246 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2464 2781 3541 1353
Size: 1111 317 760 3423
Fragments arranged by size:
3423 1111 760 317
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4198 4572 4718 387
Size: 1064 138 2609 374 146 1280
Fragments arranged by size:
2609 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2601 2766 3674 4271 5058 5326 1239
Size: 393 969 165 908 597 787 268 1524
Fragments arranged by size:
1524 969 908 787 597 393 268 165
EcoRI G'AATT_C
Cuts at: 2920 3298 2920
Size: 378 5233
FspI TGC'GCA
Cuts at: 4117 4117
Size: 5611
HaeII r_GCGC'y
Cuts at: 1678 4758 1678
Size: 3080 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2000 2032 2648 2680 2841
Size: 32 135 32 428 32 616 32 161
Cuts at: 2841 2873 4252 4284 4326 4358 1373
Size: 32 1379 32 42 32 2626
Fragments arranged by size:
2626 1379 616 428 161 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2955 3201 669
Size: 988 281 1017 246 3079
Fragments arranged by size:
3079 1017 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5611
HpaI GTT'AAC
Cuts at: 3201 3201
Size: 5611
KpnI G_GTAC'C
Cuts at: 2936 2936
Size: 5611
MfeI C'AATT_G
Cuts at: 3210 3210
Size: 5611
MluI A'CGCG_T
Cuts at: 5487 5487
Size: 5611
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2077 2830 4725 4909 1305
Size: 772 753 1895 184 2007
Fragments arranged by size:
2007 1895 772 753 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3789
Fragments arranged by size:
3789 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2280 2522 2634 3569 3928 4087 518
Size: 648 1114 242 112 935 359 159 2042
Fragments arranged by size:
2042 1114 935 648 359 242 159 112
MspA1I CmG'CkG
Cuts at: 1606 2579 3027 3704 4852 5097 1606
Size: 973 448 677 1148 245 2120
Fragments arranged by size:
2120 1148 973 677 448 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4120
Fragments arranged by size:
4120 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5611
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5611
NotI GC'GGCC_GC
Cuts at: 3058 3058
Size: 5611
NsiI A_TGCA'T
Cuts at: 2302 2463 2481 2892 2896 2302
Size: 161 18 411 4 5017
Fragments arranged by size:
5017 411 161 18 4
NspI r_CATG'y
Cuts at: 1132 1229 2461 2614 2894 2898 2969 4514 1132
Size: 97 1232 153 280 4 71 1545 2229
Fragments arranged by size:
2229 1545 1232 280 153 97 71 4
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5611
PciI A'CATG_T
Cuts at: 2610 4510 2610
Size: 1900 3711
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5611
PmeI GTTT'AAAC
Cuts at: 2915 2915
Size: 5611
PmlI CAC'GTG
Cuts at: 2231 2231
Size: 5611
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3680 3712 5193 5225 3680
Size: 32 1481 32 4066
Fragments arranged by size:
4066 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5611
PsiI TTA'TAA
Cuts at: 3181 3181
Size: 5611
PstI C_TGCA'G
Cuts at: 830 2963 830
Size: 2133 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5611
PvuII CAG'CTG
Cuts at: 2579 2579
Size: 5611
SalI G'TCGA_C
Cuts at: 1936 2953 1936
Size: 1017 4594
SbfI CC_TGCA'GG
Cuts at: 2963 2963
Size: 5611
ScaI AGT'ACT
Cuts at: 3859 5571 3859
Size: 1712 3899
SfcI C'TryA_G
Cuts at: 826 1045 2959 4094 4775 4966 826
Size: 219 1914 1135 681 191 1471
Fragments arranged by size:
1914 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5611
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5611
SmaI CCC'GGG
Cuts at: 1872 2938 1872
Size: 1066 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2261 3715 4616 4878 820
Size: 197 926 318 1454 901 262 1553
Fragments arranged by size:
1553 1454 926 901 318 262 197
SnaBI TAC'GTA
Cuts at: 493 3402 493
Size: 2909 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5611
SphI G_CATG'C
Cuts at: 1132 2894 2898 2969 1132
Size: 1762 4 71 3774
Fragments arranged by size:
3774 1762 71 4
SspI AAT'ATT
Cuts at: 5 52 2294 3535 5
Size: 47 2242 1241 2081
Fragments arranged by size:
2242 2081 1241 47
StuI AGG'CCT
Cuts at: 1517 2066 1517
Size: 549 5062
StyI C'CwwG_G
Cuts at: 513 1065 2004 3050 513
Size: 552 939 1046 3074
Fragments arranged by size:
3074 1046 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3638 3655 3808 3993 4438 325
Size: 1255 2058 17 153 185 445 1498
Fragments arranged by size:
2058 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3857 5569 96
Size: 274 80 33 51 1443 1880 1712 138
Fragments arranged by size:
1880 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5571 5603 5571
Size: 32 5579
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5611
XbaI T'CTAG_A
Cuts at: 2947 2947
Size: 5611
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5611
XmaI C'CCGG_G
Cuts at: 1870 2936 1870
Size: 1066 4545
XmnI GAAnn'nnTTC
Cuts at: 3740 3740
Size: 5611
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2857 3419 276
Size: 53 83 186 936 1323 562 2468
Fragments arranged by size:
2468 1323 936 562 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PfoI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI