Restriction map for
pFN21AE0581
(Circular) (Six-base) MAPSORT of: pFN21AE0581.seq Check: 8016 from: 1 to: 5554
pFN21AE0581 5554 bp
With 182 enzymes: SgfI *
October 20, 2009 10:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3364 278
Size: 53 83 186 936 1828 2468
Fragments arranged by size:
2468 1828 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2897 1937
Size: 960 4594
Acc65I G'GTAC_C
Cuts at: 2875 2875
Size: 5554
AclI AA'CG_TT
Cuts at: 1563 3681 4054 1563
Size: 2118 373 3063
Fragments arranged by size:
3063 2118 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3617 5001 5269 1632
Size: 1985 1384 268 1917
Fragments arranged by size:
1985 1917 1384 268
AfeI AGC'GCT
Cuts at: 2052 2052
Size: 5554
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5357
AflIII A'CryG_T
Cuts at: 1203 4453 5430 1203
Size: 3250 977 1327
Fragments arranged by size:
3250 1327 977
AgeI A'CCGG_T
Cuts at: 4385 4385
Size: 5554
AhdI GACnn_n'nnGTC
Cuts at: 4283 4283
Size: 5554
AleI CACnn'nnGTG
Cuts at: 1166 2435 1166
Size: 1269 4285
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5554
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2447 2479 2447
Size: 32 5522
AlwNI CAG_nnn'CTG
Cuts at: 2629 2827 3297 4869 2629
Size: 198 470 1572 3314
Fragments arranged by size:
3314 1572 470 198
ApaI G_GGCC'C
Cuts at: 2444 2444
Size: 5554
ApaLI G'TGCA_C
Cuts at: 3611 4767 3611
Size: 1156 4398
ApoI r'AATT_y
Cuts at: 1493 2863 3089 3241 5350 1493
Size: 1370 226 152 2109 1697
Fragments arranged by size:
2109 1697 1370 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4108 160
Size: 872 3076 1606
Fragments arranged by size:
3076 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5554
AvaI C'yCGr_G
Cuts at: 1870 1943 2879 1870
Size: 73 936 4545
Fragments arranged by size:
4545 936 73
BamHI G'GATC_C
Cuts at: 2274 2884 3251 2274
Size: 610 367 4577
Fragments arranged by size:
4577 610 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2875 4330 618
Size: 325 217 627 1088 1455 1842
Fragments arranged by size:
1842 1455 1088 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2343 2444 2706 2748 2873 1391
Size: 952 101 262 42 125 4072
Fragments arranged by size:
4072 952 262 125 101 42
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2095 928
Size: 971 196 4387
Fragments arranged by size:
4387 971 196
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3745 3779 1903
Size: 34 50 34 1724 34 3678
Fragments arranged by size:
3678 1724 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3446 4656 3446
Size: 1210 4344
BclI T'GATC_A
Cuts at: 1548 2613 1548
Size: 1065 4489
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4165 136
Size: 107 122 71 631 605 2493 1525
Fragments arranged by size:
2493 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5549 5549
Size: 5554
BlpI GC'TnA_GC
Cuts at: 2648 2971 2648
Size: 323 5231
Bme1580I G_kGCm'C
Cuts at: 1790 2444 2517 3615 4771 1790
Size: 654 73 1098 1156 2573
Fragments arranged by size:
2573 1156 1098 654 73
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5554
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3285 4243 442
Size: 416 53 83 399 24 1868 958 1753
Fragments arranged by size:
1868 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5554
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2060 2161 2336 4214 1239
Size: 821 101 175 1878 2579
Fragments arranged by size:
2579 1878 821 175 101
Bpu10I CC'TnA_GC
Cuts at: 2732 2732
Size: 5554
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3679 4544 4842 3679
Size: 865 298 4391
Fragments arranged by size:
4391 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2417 4217 882
Size: 617 918 1800 2219
Fragments arranged by size:
2219 1800 918 617
BsaAI yAC'GTr
Cuts at: 493 1204 3345 493
Size: 711 2141 2702
Fragments arranged by size:
2702 2141 711
BsaBI GATnn'nnATC
Cuts at: 5548 5548
Size: 5554
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3361 3743 4378 275
Size: 53 83 186 936 1828 382 635 1451
Fragments arranged by size:
1828 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2196 3987 4385 4659 4806 5217 2196
Size: 1791 398 274 147 411 2533
Fragments arranged by size:
2533 1791 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5329
Fragments arranged by size:
5329 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5554
BseYI C'CCAG_C
Cuts at: 2334 2495 2756 4757 5253 2334
Size: 161 261 2001 496 2635
Fragments arranged by size:
2635 2001 496 261 161
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2305 2413 2615 1163
Size: 573 569 108 202 4102
Fragments arranged by size:
4102 573 569 202 108
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3004 3765 4793 1540
Size: 275 186 1003 761 1028 2301
Fragments arranged by size:
2301 1028 1003 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2190 2343 3615 3700 4771 2190
Size: 153 1272 85 1071 2973
Fragments arranged by size:
2973 1272 1071 153 85
BsmI GAATG_Cn'
Cuts at: 3065 3158 3065
Size: 93 5461
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2190 2343 2444 2517 2706 2748 2873
Size: 399 400 153 101 73 189 42 125
Cuts at: 2873 3615 3700 4771 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 400 399 189 153 125
101 85 73 42
BspEI T'CCGG_A
Cuts at: 5217 5217
Size: 5554
BspHI T'CATG_A
Cuts at: 3443 5173 3443
Size: 1730 3824
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2435 2909 844
Size: 1591 474 3489
Fragments arranged by size:
3489 1591 474
BsrBI CCG'CTC
Cuts at: 1394 1823 3441 1394
Size: 429 1618 3507
Fragments arranged by size:
3507 1618 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4049 4223 65
Size: 1183 2801 174 1396
Fragments arranged by size:
2801 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2670 4198 4385 1700
Size: 110 860 1528 187 2869
Fragments arranged by size:
2869 1528 860 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5554
BssHII G'CGCG_C
Cuts at: 1925 2686 1925
Size: 761 4793
BssSI C'ACGA_G
Cuts at: 3614 4626 3614
Size: 1012 4542
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2629 794
Size: 1835 3719
BstBI TT'CG_AA
Cuts at: 4358 4358
Size: 5554
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5554
BstXI CCAn_nnnn'nTGG
Cuts at: 2581 2764 2581
Size: 183 5371
BstYI r'GATC_y
Cuts at: 1972 2274 2364 2884 3251 3636 3653 5105 5191
Size: 302 90 520 367 385 17 1452 86
Cuts at: 5191 5203 5549 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 520 385 367 346 302 90
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2064 513
Size: 552 999 4003
Fragments arranged by size:
4003 999 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2692 487
Size: 683 56 1466 3349
Fragments arranged by size:
3349 1466 683 56
BtsI GCAGTG_nn'
Cuts at: 3070 3864 3884 3070
Size: 794 20 4740
Fragments arranged by size:
4740 794 20
ClaI AT'CG_AT
Cuts at: 1554 2166 1554
Size: 612 4942
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5519
DraI TTT'AAA
Cuts at: 2858 2858
Size: 5554
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4561 809
Size: 507 3245 1802
Fragments arranged by size:
3245 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2535 2586 3001
Size: 54 1425 50 275 18 705 51 415
Cuts at: 3001 3890 5519 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 705 415 275 54 51
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3001 1537
Size: 275 1189 4090
Fragments arranged by size:
4090 1189 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3484 1353
Size: 2131 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4141 4515 4661 387
Size: 1064 138 2552 374 146 1280
Fragments arranged by size:
2552 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2341 2341
Size: 5554
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2060 2161 2336 3617 4214 5001 5269
Size: 393 428 101 175 1281 597 787 268
Cuts at: 5269 1239
Size: 1524
Fragments arranged by size:
1524 1281 787 597 428 393 268 175
101
EcoNI CCTnn'n_nnAGG
Cuts at: 2354 2354
Size: 5554
EcoO109I rG'GnC_Cy
Cuts at: 2441 2794 2441
Size: 353 5201
EcoRI G'AATT_C
Cuts at: 2863 3241 2863
Size: 378 5176
FspI TGC'GCA
Cuts at: 4060 4060
Size: 5554
HaeII r_GCGC'y
Cuts at: 1678 2054 2763 4701 1678
Size: 376 709 1938 2531
Fragments arranged by size:
2531 1938 709 376
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2159 2191 4195 4227 4269
Size: 32 135 32 587 32 2004 32 42
Cuts at: 4269 4301 1373
Size: 32 2626
Fragments arranged by size:
2626 2004 587 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2898 3144 669
Size: 988 281 960 246 3079
Fragments arranged by size:
3079 988 960 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5554
HpaI GTT'AAC
Cuts at: 3144 3144
Size: 5554
KpnI G_GTAC'C
Cuts at: 2879 2879
Size: 5554
MfeI C'AATT_G
Cuts at: 3153 3153
Size: 5554
MluI A'CGCG_T
Cuts at: 5430 5430
Size: 5554
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4668 4852 1305
Size: 3363 184 2007
Fragments arranged by size:
3363 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2537 2588 10
Size: 54 1425 343 705 51 2976
Fragments arranged by size:
2976 1425 705 343 54 51
MslI CAynn'nnrTG
Cuts at: 518 1166 2435 2474 2657 3512 3871 4030 518
Size: 648 1269 39 183 855 359 159 2042
Fragments arranged by size:
2042 1269 855 648 359 183 159 39
MspA1I CmG'CkG
Cuts at: 1606 2334 2827 2970 3647 4795 5040 1606
Size: 728 493 143 677 1148 245 2120
Fragments arranged by size:
2120 1148 728 677 493 245 143
NcoI C'CATG_G
Cuts at: 513 1065 2064 513
Size: 552 999 4003
Fragments arranged by size:
4003 999 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5554
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5554
NotI GC'GGCC_GC
Cuts at: 3001 3001
Size: 5554
NspI r_CATG'y
Cuts at: 1132 1229 2329 2644 2912 4457 1132
Size: 97 1100 315 268 1545 2229
Fragments arranged by size:
2229 1545 1100 315 268 97
PasI CC'CwG_GG
Cuts at: 1348 2677 1348
Size: 1329 4225
PciI A'CATG_T
Cuts at: 4453 4453
Size: 5554
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5554
PfoI T'CCnGG_A
Cuts at: 2106 2352 2106
Size: 246 5308
PmeI GTTT'AAAC
Cuts at: 2858 2858
Size: 5554
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3623 3655 5136 5168 3623
Size: 32 1481 32 4009
Fragments arranged by size:
4009 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2794 2794
Size: 5554
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5554
PsiI TTA'TAA
Cuts at: 3124 3124
Size: 5554
PspOMI G'GGCC_C
Cuts at: 2440 2440
Size: 5554
PstI C_TGCA'G
Cuts at: 830 2625 2906 830
Size: 1795 281 3478
Fragments arranged by size:
3478 1795 281
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5554
PvuII CAG'CTG
Cuts at: 2334 2334
Size: 5554
SacI G_AGCT'C
Cuts at: 2343 2343
Size: 5554
SalI G'TCGA_C
Cuts at: 1936 2896 1936
Size: 960 4594
SbfI CC_TGCA'GG
Cuts at: 2906 2906
Size: 5554
ScaI AGT'ACT
Cuts at: 3802 5514 3802
Size: 1712 3842
SfcI C'TryA_G
Cuts at: 826 1045 2621 2902 4037 4718 4909 826
Size: 219 1576 281 1135 681 191 1471
Fragments arranged by size:
1576 1471 1135 681 281 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5554
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5554
SmaI CCC'GGG
Cuts at: 1872 2881 1872
Size: 1009 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3658 4559 4821 820
Size: 197 926 1715 901 262 1553
Fragments arranged by size:
1715 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3345 493
Size: 2852 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5554
SphI G_CATG'C
Cuts at: 1132 2644 2912 1132
Size: 1512 268 3774
Fragments arranged by size:
3774 1512 268
SspI AAT'ATT
Cuts at: 5 52 3478 5
Size: 47 3426 2081
Fragments arranged by size:
3426 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5554
StyI C'CwwG_G
Cuts at: 513 1065 2064 2993 513
Size: 552 999 929 3074
Fragments arranged by size:
3074 999 929 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3581 3598 3751 3936 4381 325
Size: 1255 2001 17 153 185 445 1498
Fragments arranged by size:
2001 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3800 5512 96
Size: 274 80 33 51 1443 1823 1712 138
Fragments arranged by size:
1823 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2193 2225 5514 5546 2193
Size: 32 3289 32 2201
Fragments arranged by size:
3289 2201 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2150 1316
Size: 834 4720
XbaI T'CTAG_A
Cuts at: 2890 2890
Size: 5554
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2581 2674 2581
Size: 93 5461
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5554
XmaI C'CCGG_G
Cuts at: 1870 2879 1870
Size: 1009 4545
XmnI GAAnn'nnTTC
Cuts at: 3683 3683
Size: 5554
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3362 276
Size: 53 83 186 936 1828 2468
Fragments arranged by size:
2468 1828 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI