Restriction map for
pFN21AE0566
(Circular) (Six-base) MAPSORT of: pFN21AE0566.seq Check: 8075 from: 1 to: 5926
pFN21AE0566 5926 bp
With 182 enzymes: SgfI *
October 20, 2009 10:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3736 278
Size: 53 83 186 936 2200 2468
Fragments arranged by size:
2468 2200 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2166 2372 3269 1937
Size: 229 206 897 4594
Fragments arranged by size:
4594 897 229 206
Acc65I G'GTAC_C
Cuts at: 3247 3247
Size: 5926
AclI AA'CG_TT
Cuts at: 1563 4053 4426 1563
Size: 2490 373 3063
Fragments arranged by size:
3063 2490 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3989 5373 5641 1632
Size: 2357 1384 268 1917
Fragments arranged by size:
2357 1917 1384 268
AfeI AGC'GCT
Cuts at: 2327 2327
Size: 5926
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5729
AflIII A'CryG_T
Cuts at: 1203 2721 4825 5802 1203
Size: 1518 2104 977 1327
Fragments arranged by size:
2104 1518 1327 977
AgeI A'CCGG_T
Cuts at: 4757 4757
Size: 5926
AhdI GACnn_n'nnGTC
Cuts at: 4655 4655
Size: 5926
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5926
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5926
AlwNI CAG_nnn'CTG
Cuts at: 3669 5241 3669
Size: 1572 4354
ApaI G_GGCC'C
Cuts at: 2239 2239
Size: 5926
ApaLI G'TGCA_C
Cuts at: 3101 3983 5139 3101
Size: 882 1156 3888
Fragments arranged by size:
3888 1156 882
ApoI r'AATT_y
Cuts at: 1493 2459 2769 2849 2882 3059 3081 3155 3235
Size: 966 310 80 33 177 22 74 80
Cuts at: 3235 3461 3613 5722 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 966 310 226 177 152 80
80 74 33 22
AseI AT'TA_AT
Cuts at: 160 1032 4480 160
Size: 872 3448 1606
Fragments arranged by size:
3448 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5926
AvaI C'yCGr_G
Cuts at: 1870 1943 2119 2211 2277 2284 3251 1870
Size: 73 176 92 66 7 967 4545
Fragments arranged by size:
4545 967 176 92 73 66 7
BamHI G'GATC_C
Cuts at: 3256 3623 3256
Size: 367 5559
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2060 2180 2301 2314 3247
Size: 325 217 627 273 120 121 13 933
Cuts at: 3247 4702 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 933 627 325 273 217 121
120 13
BanII G_rGCy'C
Cuts at: 1391 2239 3245 1391
Size: 848 1006 4072
Fragments arranged by size:
4072 1006 848
BbeI G_GCGC'C
Cuts at: 2064 2184 2305 2064
Size: 120 121 5685
Fragments arranged by size:
5685 121 120
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4955
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4117 4151 1903
Size: 34 50 34 2096 34 3678
Fragments arranged by size:
3678 2096 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3818 5028 3818
Size: 1210 4716
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5926
BfrBI ATG'CAT
Cuts at: 2500 2500
Size: 5926
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4537 136
Size: 107 122 71 631 605 2865 1525
Fragments arranged by size:
2865 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5921 5921
Size: 5926
BlpI GC'TnA_GC
Cuts at: 3343 3343
Size: 5926
Bme1580I G_kGCm'C
Cuts at: 1790 2239 2319 3105 3987 5143 1790
Size: 449 80 786 882 1156 2573
Fragments arranged by size:
2573 1156 882 786 449 80
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5926
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2148 2396 2444
Size: 416 53 83 399 24 731 248 48
Cuts at: 2444 3657 4615 442
Size: 1213 958 1753
Fragments arranged by size:
1753 1213 958 731 416 399 248 83
53 48 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5926
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2058 4586 1239
Size: 819 2528 2579
Fragments arranged by size:
2579 2528 819
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4051 4916 5214 4051
Size: 865 298 4763
Fragments arranged by size:
4763 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2287 2466 4589 882
Size: 617 788 179 2123 2219
Fragments arranged by size:
2219 2123 788 617 179
BsaAI yAC'GTr
Cuts at: 493 1204 3717 493
Size: 711 2513 2702
Fragments arranged by size:
2702 2513 711
BsaBI GATnn'nnATC
Cuts at: 5920 5920
Size: 5926
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2061 2108 2181 2302
Size: 53 83 186 936 528 47 73 121
Cuts at: 2302 3733 4115 4750 275
Size: 1431 382 635 1451
Fragments arranged by size:
1451 1431 936 635 528 382 186 121
83 73 53 47
BsaWI w'CCGG_w
Cuts at: 2129 4359 4757 5031 5178 5589 2129
Size: 2230 398 274 147 411 2466
Fragments arranged by size:
2466 2230 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5701
Fragments arranged by size:
5701 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2087 1186
Size: 901 5025
BseYI C'CCAG_C
Cuts at: 2232 5129 5625 2232
Size: 2897 496 2533
Fragments arranged by size:
2897 2533 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2164 1163
Size: 573 428 4925
Fragments arranged by size:
4925 573 428
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3376 4137 5165 1540
Size: 275 186 1375 761 1028 2301
Fragments arranged by size:
2301 1375 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3105 3987 4072 5143 3105
Size: 882 85 1071 3888
Fragments arranged by size:
3888 1071 882 85
BsmI GAATG_Cn'
Cuts at: 3437 3530 3437
Size: 93 5833
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2239 2319 3105 3245 3987 4072 5143
Size: 399 449 80 786 140 742 85 1071
Cuts at: 5143 1391
Size: 2174
Fragments arranged by size:
2174 1071 786 742 449 399 140 85
80
BspEI T'CCGG_A
Cuts at: 2129 5589 2129
Size: 3460 2466
BspHI T'CATG_A
Cuts at: 3815 5545 3815
Size: 1730 4196
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2025 3281 844
Size: 1181 1256 3489
Fragments arranged by size:
3489 1256 1181
BsrBI CCG'CTC
Cuts at: 1394 1823 3813 1394
Size: 429 1990 3507
Fragments arranged by size:
3507 1990 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4421 4595 65
Size: 1183 3173 174 1396
Fragments arranged by size:
3173 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2104 2311 4570 4757 1700
Size: 110 294 207 2259 187 2869
Fragments arranged by size:
2869 2259 294 207 187 110
BsrGI T'GTAC_A
Cuts at: 96 2582 96
Size: 2486 3440
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5926
BssSI C'ACGA_G
Cuts at: 3986 4998 3986
Size: 1012 4914
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5926
BstBI TT'CG_AA
Cuts at: 4730 4730
Size: 5926
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5926
BstYI r'GATC_y
Cuts at: 1972 3256 3623 4008 4025 5477 5563 5575 5921
Size: 1284 367 385 17 1452 86 12 346
Cuts at: 5921 1972
Size: 1977
Fragments arranged by size:
1977 1452 1284 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2373 2373
Size: 5926
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5374
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5187
Fragments arranged by size:
5187 683 56
BtsI GCAGTG_nn'
Cuts at: 3099 3442 4236 4256 3099
Size: 343 794 20 4769
Fragments arranged by size:
4769 794 343 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5926
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5891
DraI TTT'AAA
Cuts at: 2464 3230 2464
Size: 766 5160
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4933 809
Size: 507 3617 1802
Fragments arranged by size:
3617 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2309 3373 4262
Size: 54 1425 50 275 18 479 1064 889
Cuts at: 4262 5891 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1064 889 479 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3373 1537
Size: 275 1561 4090
Fragments arranged by size:
4090 1561 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3856 1353
Size: 2503 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4513 4887 5033 387
Size: 1064 138 2924 374 146 1280
Fragments arranged by size:
2924 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2058 3989 4586 5373 5641 1239
Size: 393 426 1931 597 787 268 1524
Fragments arranged by size:
1931 1524 787 597 426 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2236 2862 2236
Size: 626 5300
EcoRI G'AATT_C
Cuts at: 3235 3613 3235
Size: 378 5548
FspI TGC'GCA
Cuts at: 4432 4432
Size: 5926
HaeII r_GCGC'y
Cuts at: 1678 2064 2184 2246 2305 2329 2640 5073 1678
Size: 386 120 62 59 24 311 2433 2531
Fragments arranged by size:
2531 2433 386 311 120 62 59 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4567 4599 4641 4673 1373
Size: 32 135 32 2995 32 42 32 2626
Fragments arranged by size:
2995 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3270 3516 669
Size: 988 281 1332 246 3079
Fragments arranged by size:
3079 1332 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5926
HpaI GTT'AAC
Cuts at: 3516 3516
Size: 5926
KasI G'GCGC_C
Cuts at: 2060 2180 2301 2060
Size: 120 121 5685
Fragments arranged by size:
5685 121 120
KpnI G_GTAC'C
Cuts at: 3251 3251
Size: 5926
MfeI C'AATT_G
Cuts at: 3525 3525
Size: 5926
MluI A'CGCG_T
Cuts at: 5802 5802
Size: 5926
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2408 2593 2623 5040 5224 1305
Size: 1103 185 30 2417 184 2007
Fragments arranged by size:
2417 2007 1103 185 184 30
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4104
Fragments arranged by size:
4104 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3884 4243 4402 518
Size: 648 2718 359 159 2042
Fragments arranged by size:
2718 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3342 4019 5167 5412 1606
Size: 1736 677 1148 245 2120
Fragments arranged by size:
2120 1736 1148 677 245
NarI GG'CG_CC
Cuts at: 2061 2181 2302 2061
Size: 120 121 5685
Fragments arranged by size:
5685 121 120
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5374
NdeI CA'TA_TG
Cuts at: 387 3178 387
Size: 2791 3135
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5926
NotI GC'GGCC_GC
Cuts at: 3373 3373
Size: 5926
NsiI A_TGCA'T
Cuts at: 2502 2502
Size: 5926
NspI r_CATG'y
Cuts at: 1132 1229 2095 2725 3284 4829 1132
Size: 97 866 630 559 1545 2229
Fragments arranged by size:
2229 1545 866 630 559 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5926
PciI A'CATG_T
Cuts at: 2721 4825 2721
Size: 2104 3822
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2036 1108
Size: 928 4998
PfoI T'CCnGG_A
Cuts at: 2071 2071
Size: 5926
PmeI GTTT'AAAC
Cuts at: 3230 3230
Size: 5926
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3995 4027 5508 5540 3995
Size: 32 1481 32 4381
Fragments arranged by size:
4381 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2862 2862
Size: 5926
PshAI GACnn'nnGTC
Cuts at: 1613 2337 1613
Size: 724 5202
PsiI TTA'TAA
Cuts at: 3126 3496 3126
Size: 370 5556
PspOMI G'GGCC_C
Cuts at: 2235 2235
Size: 5926
PstI C_TGCA'G
Cuts at: 830 3278 830
Size: 2448 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5926
SalI G'TCGA_C
Cuts at: 1936 3268 1936
Size: 1332 4594
SbfI CC_TGCA'GG
Cuts at: 3278 3278
Size: 5926
ScaI AGT'ACT
Cuts at: 2137 4174 5886 2137
Size: 2037 1712 2177
Fragments arranged by size:
2177 2037 1712
SfcI C'TryA_G
Cuts at: 826 1045 3274 4409 5090 5281 826
Size: 219 2229 1135 681 191 1471
Fragments arranged by size:
2229 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2062 2182 2303 2062
Size: 120 121 5685
Fragments arranged by size:
5685 121 120
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5926
SgrAI Cr'CCGG_yG
Cuts at: 1700 2104 1700
Size: 404 5522
SmaI CCC'GGG
Cuts at: 1872 2279 3253 1872
Size: 407 974 4545
Fragments arranged by size:
4545 974 407
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2119 4030 4931 5193 820
Size: 197 926 176 1911 901 262 1553
Fragments arranged by size:
1911 1553 926 901 262 197 176
SnaBI TAC'GTA
Cuts at: 493 3717 493
Size: 3224 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5926
SphI G_CATG'C
Cuts at: 1132 2095 3284 1132
Size: 963 1189 3774
Fragments arranged by size:
3774 1189 963
SspI AAT'ATT
Cuts at: 5 52 2928 3137 3850 5
Size: 47 2876 209 713 2081
Fragments arranged by size:
2876 2081 713 209 47
StuI AGG'CCT
Cuts at: 1517 2117 1517
Size: 600 5326
StyI C'CwwG_G
Cuts at: 513 1065 3365 513
Size: 552 2300 3074
Fragments arranged by size:
3074 2300 552
SwaI ATTT'AAAT
Cuts at: 2464 2464
Size: 5926
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2130 2734 3953 3970 4123 4308 4753
Size: 1255 550 604 1219 17 153 185 445
Cuts at: 4753 325
Size: 1498
Fragments arranged by size:
1498 1255 1219 604 550 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2135 2522 2582
Size: 274 80 33 51 1443 158 387 60
Cuts at: 2582 4172 5884 96
Size: 1590 1712 138
Fragments arranged by size:
1712 1590 1443 387 274 158 138 80
60 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5886 5918 5886
Size: 32 5894
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5926
XbaI T'CTAG_A
Cuts at: 3262 3262
Size: 5926
XhoI C'TCGA_G
Cuts at: 1943 2119 1943
Size: 176 5750
XmaI C'CCGG_G
Cuts at: 1870 2277 3251 1870
Size: 407 974 4545
Fragments arranged by size:
4545 974 407
XmnI GAAnn'nnTTC
Cuts at: 2433 4055 2433
Size: 1622 4304
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3734 276
Size: 53 83 186 936 2200 2468
Fragments arranged by size:
2468 2200 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI SwaI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BplI Bpu10I
BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI NaeI NgoMIV NruI PacI PmlI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SrfI XcmI