Restriction map for
pFN21AE0561
(Circular) (Six-base) MAPSORT of: pFN21AE0561.seq Check: 5083 from: 1 to: 5704
pFN21AE0561 5704 bp
With 182 enzymes: SgfI *
October 20, 2009 10:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3514 278
Size: 53 83 186 936 1978 2468
Fragments arranged by size:
2468 1978 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2471 3047 1937
Size: 534 576 4594
Fragments arranged by size:
4594 576 534
Acc65I G'GTAC_C
Cuts at: 3025 3025
Size: 5704
AclI AA'CG_TT
Cuts at: 1563 3831 4204 1563
Size: 2268 373 3063
Fragments arranged by size:
3063 2268 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2056 2297 2627 3767 5151 5419 1632
Size: 424 241 330 1140 1384 268 1917
Fragments arranged by size:
1917 1384 1140 424 330 268 241
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5507
AflIII A'CryG_T
Cuts at: 1203 4603 5580 1203
Size: 3400 977 1327
Fragments arranged by size:
3400 1327 977
AgeI A'CCGG_T
Cuts at: 2616 4535 2616
Size: 1919 3785
AhdI GACnn_n'nnGTC
Cuts at: 4433 4433
Size: 5704
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5704
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5704
AlwNI CAG_nnn'CTG
Cuts at: 2992 3447 5019 2992
Size: 455 1572 3677
Fragments arranged by size:
3677 1572 455
ApaLI G'TGCA_C
Cuts at: 3761 4917 3761
Size: 1156 4548
ApoI r'AATT_y
Cuts at: 1493 3013 3239 3391 5500 1493
Size: 1520 226 152 2109 1697
Fragments arranged by size:
2109 1697 1520 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4258 160
Size: 872 3226 1606
Fragments arranged by size:
3226 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5704
AvaI C'yCGr_G
Cuts at: 1870 1943 3029 1870
Size: 73 1086 4545
Fragments arranged by size:
4545 1086 73
BamHI G'GATC_C
Cuts at: 3034 3401 3034
Size: 367 5337
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2232 2653 3025 4480 618
Size: 325 217 627 445 421 372 1455 1842
Fragments arranged by size:
1842 1455 627 445 421 372 325 217
BanII G_rGCy'C
Cuts at: 1391 2337 3023 1391
Size: 946 686 4072
Fragments arranged by size:
4072 946 686
BbeI G_GCGC'C
Cuts at: 2236 2236
Size: 5704
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2192 2285 928
Size: 971 293 93 4347
Fragments arranged by size:
4347 971 293 93
BbvCI CC'TCA_GC
Cuts at: 2193 2193
Size: 5704
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3895 3929 1903
Size: 34 50 34 1874 34 3678
Fragments arranged by size:
3678 1874 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3596 4806 3596
Size: 1210 4494
BclI T'GATC_A
Cuts at: 1548 2698 1548
Size: 1150 4554
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2372 4315 136
Size: 107 122 71 631 605 700 1943 1525
Fragments arranged by size:
1943 1525 700 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5699 5699
Size: 5704
BlpI GC'TnA_GC
Cuts at: 3121 3121
Size: 5704
Bme1580I G_kGCm'C
Cuts at: 1790 3765 4921 1790
Size: 1975 1156 2573
Fragments arranged by size:
2573 1975 1156
BmgBI CAC'GTC
Cuts at: 1318 2792 1318
Size: 1474 4230
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2435 3435 4393
Size: 416 53 83 399 24 1018 1000 958
Cuts at: 4393 442
Size: 1753
Fragments arranged by size:
1753 1018 1000 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5704
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3014 4364 1239
Size: 1775 1350 2579
Fragments arranged by size:
2579 1775 1350
Bpu10I CC'TnA_GC
Cuts at: 2193 2193
Size: 5704
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2015 2252 3829 4694 4992 2015
Size: 237 1577 865 298 2727
Fragments arranged by size:
2727 1577 865 298 237
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2975 4367 882
Size: 617 1476 1392 2219
Fragments arranged by size:
2219 1476 1392 617
BsaAI yAC'GTr
Cuts at: 493 1204 2939 3495 493
Size: 711 1735 556 2702
Fragments arranged by size:
2702 1735 711 556
BsaBI GATnn'nnATC
Cuts at: 5698 5698
Size: 5704
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2233 3511 3893 4528
Size: 53 83 186 936 700 1278 382 635
Cuts at: 4528 275
Size: 1451
Fragments arranged by size:
1451 1278 936 700 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2616 4137 4535 4809 4956 5367 2616
Size: 1521 398 274 147 411 2953
Fragments arranged by size:
2953 1521 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5479
Fragments arranged by size:
5479 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5704
BseYI C'CCAG_C
Cuts at: 2297 4907 5403 2297
Size: 2610 496 2598
Fragments arranged by size:
2610 2598 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2593 1163
Size: 573 857 4274
Fragments arranged by size:
4274 857 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2206 3154 3915 4943 1540
Size: 275 186 205 948 761 1028 2301
Fragments arranged by size:
2301 1028 948 761 275 205 186
BsiHKAI G_wGCw'C
Cuts at: 3765 3850 4921 3765
Size: 85 1071 4548
Fragments arranged by size:
4548 1071 85
BsmI GAATG_Cn'
Cuts at: 3215 3308 3215
Size: 93 5611
BsmBI CGTCTCn'nnnn_
Cuts at: 2678 2678
Size: 5704
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2337 3023 3765 3850 4921 1391
Size: 399 547 686 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 686 547 399 85
BspEI T'CCGG_A
Cuts at: 5367 5367
Size: 5704
BspHI T'CATG_A
Cuts at: 2284 3593 5323 2284
Size: 1309 1730 2665
Fragments arranged by size:
2665 1730 1309
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2674 3059 844
Size: 1830 385 3489
Fragments arranged by size:
3489 1830 385
BsrBI CCG'CTC
Cuts at: 1394 1823 3591 1394
Size: 429 1768 3507
Fragments arranged by size:
3507 1768 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4199 4373 65
Size: 1183 2951 174 1396
Fragments arranged by size:
2951 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2208 2616 4348 4535 1700
Size: 110 398 408 1732 187 2869
Fragments arranged by size:
2869 1732 408 398 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5704
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5704
BssSI C'ACGA_G
Cuts at: 3764 4776 3764
Size: 1012 4692
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2653 794
Size: 1859 3845
BstBI TT'CG_AA
Cuts at: 4508 4508
Size: 5704
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5704
BstXI CCAn_nnnn'nTGG
Cuts at: 2330 2330
Size: 5704
BstYI r'GATC_y
Cuts at: 1972 3034 3401 3786 3803 5255 5341 5353 5699
Size: 1062 367 385 17 1452 86 12 346
Cuts at: 5699 1972
Size: 1977
Fragments arranged by size:
1977 1452 1062 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2377 2377
Size: 5704
BtgI C'CryG_G
Cuts at: 513 1065 2344 513
Size: 552 1279 3873
Fragments arranged by size:
3873 1279 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4965
Fragments arranged by size:
4965 683 56
BtsI GCAGTG_nn'
Cuts at: 3220 4014 4034 3220
Size: 794 20 4890
Fragments arranged by size:
4890 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5704
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2083 2118 529
Size: 35 1519 35 4115
Fragments arranged by size:
4115 1519 35 35
DraI TTT'AAA
Cuts at: 3008 3008
Size: 5704
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4711 809
Size: 507 3395 1802
Fragments arranged by size:
3395 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2203 2356 3151
Size: 54 1425 50 275 18 373 153 795
Cuts at: 3151 4040 5669 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 795 373 275 153 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2203 3151 1537
Size: 275 391 948 4090
Fragments arranged by size:
4090 948 391 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3634 1353
Size: 2281 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4291 4665 4811 387
Size: 1064 138 2702 374 146 1280
Fragments arranged by size:
2702 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2056 2297 2627 3014 3767 4364 5151
Size: 393 424 241 330 387 753 597 787
Cuts at: 5151 5419 1239
Size: 268 1524
Fragments arranged by size:
1524 787 753 597 424 393 387 330
268 241
EcoNI CCTnn'n_nnAGG
Cuts at: 2196 2221 2196
Size: 25 5679
EcoO109I rG'GnC_Cy
Cuts at: 2026 2478 2815 2026
Size: 452 337 4915
Fragments arranged by size:
4915 452 337
EcoRI G'AATT_C
Cuts at: 3013 3391 3013
Size: 378 5326
FspI TGC'GCA
Cuts at: 2633 4210 2633
Size: 1577 4127
FspAI rTGC'GCAy
Cuts at: 2633 2633
Size: 5704
HaeII r_GCGC'y
Cuts at: 1678 2236 4851 1678
Size: 558 2615 2531
Fragments arranged by size:
2615 2531 558
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2869 2901 4345 4377 4419
Size: 32 135 32 1297 32 1444 32 42
Cuts at: 4419 4451 1373
Size: 32 2626
Fragments arranged by size:
2626 1444 1297 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2110 2731 3048 3294 669
Size: 988 281 172 621 317 246 3079
Fragments arranged by size:
3079 988 621 317 281 246 172
HindIII A'AGCT_T
Cuts at: 748 2270 748
Size: 1522 4182
HpaI GTT'AAC
Cuts at: 3294 3294
Size: 5704
KasI G'GCGC_C
Cuts at: 2232 2232
Size: 5704
KpnI G_GTAC'C
Cuts at: 3029 3029
Size: 5704
MfeI C'AATT_G
Cuts at: 3303 3303
Size: 5704
MluI A'CGCG_T
Cuts at: 5580 5580
Size: 5704
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4818 5002 1305
Size: 3513 184 2007
Fragments arranged by size:
3513 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2358 10
Size: 54 1425 343 526 3356
Fragments arranged by size:
3356 1425 526 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2328 2549 3662 4021 4180 518
Size: 648 1162 221 1113 359 159 2042
Fragments arranged by size:
2042 1162 1113 648 359 221 159
MspA1I CmG'CkG
Cuts at: 1606 3120 3797 4945 5190 1606
Size: 1514 677 1148 245 2120
Fragments arranged by size:
2120 1514 1148 677 245
NarI GG'CG_CC
Cuts at: 2233 2233
Size: 5704
NcoI C'CATG_G
Cuts at: 513 1065 2344 513
Size: 552 1279 3873
Fragments arranged by size:
3873 1279 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5704
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5704
NotI GC'GGCC_GC
Cuts at: 2203 3151 2203
Size: 948 4756
NspI r_CATG'y
Cuts at: 1132 1229 3062 4607 1132
Size: 97 1833 1545 2229
Fragments arranged by size:
2229 1833 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5704
PciI A'CATG_T
Cuts at: 4603 4603
Size: 5704
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2758 2992 1108
Size: 1650 234 3820
Fragments arranged by size:
3820 1650 234
PmeI GTTT'AAAC
Cuts at: 3008 3008
Size: 5704
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3773 3805 5286 5318 3773
Size: 32 1481 32 4159
Fragments arranged by size:
4159 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2026 2026
Size: 5704
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5704
PsiI TTA'TAA
Cuts at: 3274 3274
Size: 5704
PstI C_TGCA'G
Cuts at: 830 3056 830
Size: 2226 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5704
SalI G'TCGA_C
Cuts at: 1936 3046 1936
Size: 1110 4594
SbfI CC_TGCA'GG
Cuts at: 3056 3056
Size: 5704
ScaI AGT'ACT
Cuts at: 3952 5664 3952
Size: 1712 3992
SexAI A'CCwGG_T
Cuts at: 2118 2118
Size: 5704
SfcI C'TryA_G
Cuts at: 826 1045 2248 2472 2827 3052 4187 4868 5059
Size: 219 1203 224 355 225 1135 681 191
Cuts at: 5059 826
Size: 1471
Fragments arranged by size:
1471 1203 1135 681 355 225 224 219
191
SfoI GGC'GCC
Cuts at: 2234 2234
Size: 5704
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5704
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5704
SmaI CCC'GGG
Cuts at: 1872 3031 1872
Size: 1159 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2030 2267 3808 4709 4971 820
Size: 197 926 87 237 1541 901 262 1553
Fragments arranged by size:
1553 1541 926 901 262 237 197 87
SnaBI TAC'GTA
Cuts at: 493 2939 3495 493
Size: 2446 556 2702
Fragments arranged by size:
2702 2446 556
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5704
SphI G_CATG'C
Cuts at: 1132 3062 1132
Size: 1930 3774
SspI AAT'ATT
Cuts at: 5 52 3628 5
Size: 47 3576 2081
Fragments arranged by size:
3576 2081 47
StuI AGG'CCT
Cuts at: 1517 2375 1517
Size: 858 4846
StyI C'CwwG_G
Cuts at: 513 1065 2344 3143 513
Size: 552 1279 799 3074
Fragments arranged by size:
3074 1279 799 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3731 3748 3901 4086 4531 325
Size: 1255 2151 17 153 185 445 1498
Fragments arranged by size:
2151 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3950 5662 96
Size: 274 80 33 51 1443 1973 1712 138
Fragments arranged by size:
1973 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5664 5696 5664
Size: 32 5672
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5704
XbaI T'CTAG_A
Cuts at: 3040 3040
Size: 5704
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2351 2351
Size: 5704
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5704
XmaI C'CCGG_G
Cuts at: 1870 3029 1870
Size: 1159 4545
XmnI GAAnn'nnTTC
Cuts at: 2747 3833 2747
Size: 1086 4618
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3512 276
Size: 53 83 186 936 1978 2468
Fragments arranged by size:
2468 1978 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BplI BsiWI BstZ17I DraIII EcoICRI EcoRV FalI FseI
NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SfiI SrfI SwaI