Restriction map for
pFN21AE0560
(Circular) (Six-base) MAPSORT of: pFN21AE0560.seq Check: 8969 from: 1 to: 6097
pFN21AE0560 6097 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3907 278
Size: 53 83 186 936 2371 2468
Fragments arranged by size:
2468 2371 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2763 3440 1937
Size: 826 677 4594
Fragments arranged by size:
4594 826 677
Acc65I G'GTAC_C
Cuts at: 3418 3418
Size: 6097
AclI AA'CG_TT
Cuts at: 1563 4224 4597 1563
Size: 2661 373 3063
Fragments arranged by size:
3063 2661 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4160 5544 5812 1632
Size: 2528 1384 268 1917
Fragments arranged by size:
2528 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2044 2444 820
Size: 197 1027 400 4473
Fragments arranged by size:
4473 1027 400 197
AflIII A'CryG_T
Cuts at: 1203 4996 5973 1203
Size: 3793 977 1327
Fragments arranged by size:
3793 1327 977
AgeI A'CCGG_T
Cuts at: 4928 4928
Size: 6097
AhdI GACnn_n'nnGTC
Cuts at: 4826 4826
Size: 6097
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6097
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6097
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2828 2860 2828
Size: 32 6065
AlwNI CAG_nnn'CTG
Cuts at: 2638 2950 2971 3115 3840 5412 2638
Size: 312 21 144 725 1572 3323
Fragments arranged by size:
3323 1572 725 312 144 21
ApaI G_GGCC'C
Cuts at: 3067 3067
Size: 6097
ApaLI G'TGCA_C
Cuts at: 4154 5310 4154
Size: 1156 4941
ApoI r'AATT_y
Cuts at: 1493 2135 3199 3244 3406 3632 3784 5893 1493
Size: 642 1064 45 162 226 152 2109 1697
Fragments arranged by size:
2109 1697 1064 642 226 162 152 45
AseI AT'TA_AT
Cuts at: 160 1032 4651 160
Size: 872 3619 1606
Fragments arranged by size:
3619 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6097
AvaI C'yCGr_G
Cuts at: 1870 1943 2755 3422 1870
Size: 73 812 667 4545
Fragments arranged by size:
4545 812 667 73
BamHI G'GATC_C
Cuts at: 2027 3427 3794 2027
Size: 1400 367 4330
Fragments arranged by size:
4330 1400 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2195 3418 4873 618
Size: 325 217 627 408 1223 1455 1842
Fragments arranged by size:
1842 1455 1223 627 408 325 217
BanII G_rGCy'C
Cuts at: 1391 2323 2553 3067 3416 1391
Size: 932 230 514 349 4072
Fragments arranged by size:
4072 932 514 349 230
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5126
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2792 2826 4288 4322 1903
Size: 34 855 34 1462 34 3678
Fragments arranged by size:
3678 1462 855 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3989 5199 3989
Size: 1210 4887
BclI T'GATC_A
Cuts at: 1548 2404 1548
Size: 856 5241
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2513 4708 136
Size: 107 122 71 631 605 841 2195 1525
Fragments arranged by size:
2195 1525 841 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2877 6092 2877
Size: 3215 2882
BlpI GC'TnA_GC
Cuts at: 3514 3514
Size: 6097
Bme1580I G_kGCm'C
Cuts at: 1790 2200 3067 4158 5314 1790
Size: 410 867 1091 1156 2573
Fragments arranged by size:
2573 1156 1091 867 410
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6097
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3828 4786 442
Size: 416 53 83 399 24 2411 958 1753
Fragments arranged by size:
2411 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6097
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2073 2345 2972 3014 3089 3137 4757 1239
Size: 834 272 627 42 75 48 1620 2579
Fragments arranged by size:
2579 1620 834 627 272 75 48 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4222 5087 5385 4222
Size: 865 298 4934
Fragments arranged by size:
4934 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3005 3092 3161 4760 882
Size: 617 1506 87 69 1599 2219
Fragments arranged by size:
2219 1599 1506 617 87 69
BsaAI yAC'GTr
Cuts at: 493 1204 3888 493
Size: 711 2684 2702
Fragments arranged by size:
2702 2684 711
BsaBI GATnn'nnATC
Cuts at: 6091 6091
Size: 6097
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3904 4286 4921 275
Size: 53 83 186 936 2371 382 635 1451
Fragments arranged by size:
2371 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4530 4928 5202 5349 5760 4530
Size: 398 274 147 411 4867
Fragments arranged by size:
4867 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2828 2858 1178
Size: 30 165 30 1425 30 4417
Fragments arranged by size:
4417 1425 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2237 2342 2452 2675 2708 3086 3122 1186
Size: 1051 105 110 223 33 378 36 4161
Fragments arranged by size:
4161 1051 378 223 110 105 36 33
BseYI C'CCAG_C
Cuts at: 2720 5300 5796 2720
Size: 2580 496 3021
Fragments arranged by size:
3021 2580 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2106 3065 1163
Size: 573 370 959 4195
Fragments arranged by size:
4195 959 573 370
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3547 4308 5336 1540
Size: 275 186 1546 761 1028 2301
Fragments arranged by size:
2301 1546 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2323 2553 3002 4158 4243 5314 2323
Size: 230 449 1156 85 1071 3106
Fragments arranged by size:
3106 1156 1071 449 230 85
BsmI GAATG_Cn'
Cuts at: 3608 3701 3608
Size: 93 6004
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2200 2323 2553 3002 3067 3416 4158
Size: 399 410 123 230 449 65 349 742
Cuts at: 4158 4243 5314 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 449 410 399 349 230
123 85 65
BspEI T'CCGG_A
Cuts at: 5760 5760
Size: 6097
BspHI T'CATG_A
Cuts at: 2407 3986 5716 2407
Size: 1579 1730 2788
Fragments arranged by size:
2788 1730 1579
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3452 844
Size: 2608 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2795 3984 1394
Size: 429 972 1189 3507
Fragments arranged by size:
3507 1189 972 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4592 4766 65
Size: 1183 3344 174 1396
Fragments arranged by size:
3344 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4741 4928 1700
Size: 110 2931 187 2869
Fragments arranged by size:
2931 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6097
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6097
BssSI C'ACGA_G
Cuts at: 4157 5169 4157
Size: 1012 5085
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2093 794
Size: 1299 4798
BstBI TT'CG_AA
Cuts at: 4901 4901
Size: 6097
BstEII G'GTnAC_C
Cuts at: 1184 2266 1184
Size: 1082 5015
BstXI CCAn_nnnn'nTGG
Cuts at: 2192 2192
Size: 6097
BstYI r'GATC_y
Cuts at: 1972 2027 2060 2387 2440 2877 2955 3045 3427
Size: 55 33 327 53 437 78 90 382
Cuts at: 3427 3794 4179 4196 5648 5734 5746 6092 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 437 385 382 367 346 327
90 86 78 55 53 33 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2395 513
Size: 552 1330 4215
Fragments arranged by size:
4215 1330 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5358
Fragments arranged by size:
5358 683 56
BtsI GCAGTG_nn'
Cuts at: 2110 2173 2717 3613 4407 4427 2110
Size: 63 544 896 794 20 3780
Fragments arranged by size:
3780 896 794 544 63 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6097
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6062
DraI TTT'AAA
Cuts at: 3401 3401
Size: 6097
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5104 809
Size: 507 3788 1802
Fragments arranged by size:
3788 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2976 3544 4433
Size: 54 1425 50 275 18 1146 568 889
Cuts at: 4433 6062 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1146 889 568 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3544 1537
Size: 275 1732 4090
Fragments arranged by size:
4090 1732 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2685 2849 3288 4027 1353
Size: 1332 164 439 739 3423
Fragments arranged by size:
3423 1332 739 439 164
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4684 5058 5204 387
Size: 1064 138 3095 374 146 1280
Fragments arranged by size:
3095 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2321 2551 2321
Size: 230 5867
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2073 2345 2972 3014 3089 3137 4160
Size: 393 441 272 627 42 75 48 1023
Cuts at: 4160 4757 5544 5812 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1023 787 627 597 441 393 272
268 75 48 42
EcoO109I rG'GnC_Cy
Cuts at: 3063 3064 3063
Size: 1 6096
EcoRI G'AATT_C
Cuts at: 3244 3406 3784 3244
Size: 162 378 5557
Fragments arranged by size:
5557 378 162
EcoRV GAT'ATC
Cuts at: 2423 2423
Size: 6097
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3166 3408 3166
Size: 242 5855
FspI TGC'GCA
Cuts at: 4603 4603
Size: 6097
HaeII r_GCGC'y
Cuts at: 1678 2862 5244 1678
Size: 1184 2382 2531
Fragments arranged by size:
2531 2382 1184
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2656 2688 2689 2721 3037
Size: 32 135 32 1084 32 1 32 316
Cuts at: 3037 3069 4738 4770 4812 4844 1373
Size: 32 1669 32 42 32 2626
Fragments arranged by size:
2626 1669 1084 316 135 42 32 32
32 32 32 32 32 1
HincII GTy'rAC
Cuts at: 669 1657 1938 3441 3687 669
Size: 988 281 1503 246 3079
Fragments arranged by size:
3079 1503 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6097
HpaI GTT'AAC
Cuts at: 3687 3687
Size: 6097
KpnI G_GTAC'C
Cuts at: 3422 3422
Size: 6097
MfeI C'AATT_G
Cuts at: 2433 3696 2433
Size: 1263 4834
MluI A'CGCG_T
Cuts at: 5973 5973
Size: 6097
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5211 5395 1305
Size: 3906 184 2007
Fragments arranged by size:
3906 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2978 10
Size: 54 1425 343 1146 3129
Fragments arranged by size:
3129 1425 1146 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2190 2927 4055 4414 4573 518
Size: 648 1024 737 1128 359 159 2042
Fragments arranged by size:
2042 1128 1024 737 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2962 3037 3079 3513 4190 5338 5583 1606
Size: 1356 75 42 434 677 1148 245 2120
Fragments arranged by size:
2120 1356 1148 677 434 245 75 42
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5545
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6097
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6097
NotI GC'GGCC_GC
Cuts at: 3544 3544
Size: 6097
NspI r_CATG'y
Cuts at: 1132 1229 2039 2112 2791 3455 5000 1132
Size: 97 810 73 679 664 1545 2229
Fragments arranged by size:
2229 1545 810 679 664 97 73
PasI CC'CwG_GG
Cuts at: 1348 3239 1348
Size: 1891 4206
PciI A'CATG_T
Cuts at: 4996 4996
Size: 6097
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3115 1108
Size: 2007 4090
PfoI T'CCnGG_A
Cuts at: 2601 2601
Size: 6097
PmeI GTTT'AAAC
Cuts at: 3401 3401
Size: 6097
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2828 2860 4166 4198 5679 5711 2828
Size: 32 1306 32 1481 32 3214
Fragments arranged by size:
3214 1481 1306 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6097
PsiI TTA'TAA
Cuts at: 3667 3667
Size: 6097
PspOMI G'GGCC_C
Cuts at: 3063 3063
Size: 6097
PstI C_TGCA'G
Cuts at: 830 2496 2624 2712 2871 2946 2967 3449 830
Size: 1666 128 88 159 75 21 482 3478
Fragments arranged by size:
3478 1666 482 159 128 88 75 21
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6097
PvuII CAG'CTG
Cuts at: 2962 3037 3079 2962
Size: 75 42 5980
Fragments arranged by size:
5980 75 42
SacI G_AGCT'C
Cuts at: 2323 2553 2323
Size: 230 5867
SalI G'TCGA_C
Cuts at: 1936 3439 1936
Size: 1503 4594
SapI GCTCTTCn'nnn_
Cuts at: 2849 3288 2849
Size: 439 5658
SbfI CC_TGCA'GG
Cuts at: 3449 3449
Size: 6097
ScaI AGT'ACT
Cuts at: 4345 6057 4345
Size: 1712 4385
SexAI A'CCwGG_T
Cuts at: 3084 3084
Size: 6097
SfcI C'TryA_G
Cuts at: 826 1045 2413 2492 2620 2708 2867 2942 2963
Size: 219 1368 79 128 88 159 75 21
Cuts at: 2963 3445 4580 5261 5452 826
Size: 482 1135 681 191 1471
Fragments arranged by size:
1471 1368 1135 681 482 219 191 159
128 88 79 75 21
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6097
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6097
SmaI CCC'GGG
Cuts at: 1872 2757 3424 1872
Size: 885 667 4545
Fragments arranged by size:
4545 885 667
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2044 2444 4201 5102 5364 820
Size: 197 926 101 400 1757 901 262 1553
Fragments arranged by size:
1757 1553 926 901 400 262 197 101
SnaBI TAC'GTA
Cuts at: 493 3888 493
Size: 3395 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6097
SphI G_CATG'C
Cuts at: 1132 3455 1132
Size: 2323 3774
SspI AAT'ATT
Cuts at: 5 52 4021 5
Size: 47 3969 2081
Fragments arranged by size:
3969 2081 47
StuI AGG'CCT
Cuts at: 1517 2840 1517
Size: 1323 4774
StyI C'CwwG_G
Cuts at: 513 1065 2148 2200 3536 513
Size: 552 1083 52 1336 3074
Fragments arranged by size:
3074 1336 1083 552 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2642 3029 3116 4124 4141 4294 4479
Size: 1255 1062 387 87 1008 17 153 185
Cuts at: 4479 4924 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1062 1008 445 387 185 153
87 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4343 6055 96
Size: 274 80 33 51 1443 2366 1712 138
Fragments arranged by size:
2366 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6057 6089 6057
Size: 32 6065
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6097
XbaI T'CTAG_A
Cuts at: 3433 3433
Size: 6097
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2021 3271 2021
Size: 1250 4847
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6097
XmaI C'CCGG_G
Cuts at: 1870 2755 3422 1870
Size: 885 667 4545
Fragments arranged by size:
4545 885 667
XmnI GAAnn'nnTTC
Cuts at: 4226 4226
Size: 6097
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3905 276
Size: 53 83 186 936 2371 2468
Fragments arranged by size:
2468 2371 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI
SfiI SfoI SrfI SwaI