Restriction map for
pFN21AE0557
(Circular) (Six-base) MAPSORT of: pFN21AE0557.seq Check: 1859 from: 1 to: 6055
pFN21AE0557 6055 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3865 278
Size: 53 83 186 936 2329 2468
Fragments arranged by size:
2468 2329 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3291 3398 1937
Size: 1354 107 4594
Fragments arranged by size:
4594 1354 107
Acc65I G'GTAC_C
Cuts at: 3376 3376
Size: 6055
AclI AA'CG_TT
Cuts at: 1563 4182 4555 1563
Size: 2619 373 3063
Fragments arranged by size:
3063 2619 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2927 4118 5502 5770 1632
Size: 1295 1191 1384 268 1917
Fragments arranged by size:
1917 1384 1295 1191 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5858
AflIII A'CryG_T
Cuts at: 1203 4954 5931 1203
Size: 3751 977 1327
Fragments arranged by size:
3751 1327 977
AgeI A'CCGG_T
Cuts at: 4886 4886
Size: 6055
AhdI GACnn_n'nnGTC
Cuts at: 3153 4784 3153
Size: 1631 4424
AleI CACnn'nnGTG
Cuts at: 1166 2544 1166
Size: 1378 4677
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6055
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2807 2839 2807
Size: 32 6023
AlwNI CAG_nnn'CTG
Cuts at: 3094 3112 3798 5370 3094
Size: 18 686 1572 3779
Fragments arranged by size:
3779 1572 686 18
ApaI G_GGCC'C
Cuts at: 3046 3046
Size: 6055
ApaLI G'TGCA_C
Cuts at: 4112 5268 4112
Size: 1156 4899
ApoI r'AATT_y
Cuts at: 1493 2104 2354 3364 3590 3742 5851 1493
Size: 611 250 1010 226 152 2109 1697
Fragments arranged by size:
2109 1697 1010 611 250 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4609 160
Size: 872 3577 1606
Fragments arranged by size:
3577 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6055
AvaI C'yCGr_G
Cuts at: 1870 1943 2735 3380 1870
Size: 73 792 645 4545
Fragments arranged by size:
4545 792 645 73
BamHI G'GATC_C
Cuts at: 3385 3752 3385
Size: 367 5688
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3376 4831 618
Size: 325 217 627 1589 1455 1842
Fragments arranged by size:
1842 1589 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2226 3046 3374 1391
Size: 835 820 328 4072
Fragments arranged by size:
4072 835 820 328
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5084
BbvCI CC'TCA_GC
Cuts at: 2884 2884
Size: 6055
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2771 2805 4246 4280 1903
Size: 34 834 34 1441 34 3678
Fragments arranged by size:
3678 1441 834 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3947 5157 3947
Size: 1210 4845
BclI T'GATC_A
Cuts at: 1548 2383 1548
Size: 835 5220
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4666 136
Size: 107 122 71 631 605 2994 1525
Fragments arranged by size:
2994 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2608 2856 6050 2608
Size: 248 3194 2613
Fragments arranged by size:
3194 2613 248
BlpI GC'TnA_GC
Cuts at: 3472 3472
Size: 6055
Bme1580I G_kGCm'C
Cuts at: 1790 3046 4116 5272 1790
Size: 1256 1070 1156 2573
Fragments arranged by size:
2573 1256 1156 1070
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6055
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3786 4744 442
Size: 416 53 83 399 24 2369 958 1753
Fragments arranged by size:
2369 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6055
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2324 2345 2633 2721 2993 3068 3116 4715
Size: 1085 21 288 88 272 75 48 1599
Cuts at: 4715 1239
Size: 2579
Fragments arranged by size:
2579 1599 1085 288 272 88 75 48
21
Bpu10I CC'TnA_GC
Cuts at: 2884 2884
Size: 6055
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2498 3302 4180 5045 5343 2498
Size: 804 878 865 298 3210
Fragments arranged by size:
3210 878 865 804 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2984 3071 3140 4718 882
Size: 617 1485 87 69 1578 2219
Fragments arranged by size:
2219 1578 1485 617 87 69
BsaAI yAC'GTr
Cuts at: 493 1204 3846 493
Size: 711 2642 2702
Fragments arranged by size:
2702 2642 711
BsaBI GATnn'nnATC
Cuts at: 6049 6049
Size: 6055
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3862 4244 4879 275
Size: 53 83 186 936 2329 382 635 1451
Fragments arranged by size:
2329 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4488 4886 5160 5307 5718 4488
Size: 398 274 147 411 4825
Fragments arranged by size:
4825 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2026 2056 2606 2636 2694
Size: 30 165 30 623 30 550 30 58
Cuts at: 2694 2724 2807 2837 1178
Size: 30 83 30 4396
Fragments arranged by size:
4396 623 550 165 83 58 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2321 2654 3065 3183 3299 1186
Size: 1135 333 411 118 116 3942
Fragments arranged by size:
3942 1135 411 333 118 116
BseYI C'CCAG_C
Cuts at: 3250 5258 5754 3250
Size: 2008 496 3551
Fragments arranged by size:
3551 2008 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2620 3044 1163
Size: 573 884 424 4174
Fragments arranged by size:
4174 884 573 424
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3505 4266 5294 1540
Size: 275 186 1504 761 1028 2301
Fragments arranged by size:
2301 1504 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2981 4116 4201 5272 2981
Size: 1135 85 1071 3764
Fragments arranged by size:
3764 1135 1071 85
BsmI GAATG_Cn'
Cuts at: 3566 3659 3566
Size: 93 5962
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2226 2981 3046 3374 4116 4201 5272
Size: 399 436 755 65 328 742 85 1071
Cuts at: 5272 1391
Size: 2174
Fragments arranged by size:
2174 1071 755 742 436 399 328 85
65
BspEI T'CCGG_A
Cuts at: 5718 5718
Size: 6055
BspHI T'CATG_A
Cuts at: 2386 3944 5674 2386
Size: 1558 1730 2767
Fragments arranged by size:
2767 1730 1558
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3410 844
Size: 2566 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2218 3942 1394
Size: 429 395 1724 3507
Fragments arranged by size:
3507 1724 429 395
BsrDI GCAATG_nn'
Cuts at: 65 1248 2242 2461 3358 4550 4724 65
Size: 1183 994 219 897 1192 174 1396
Fragments arranged by size:
1396 1192 1183 994 897 219 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4699 4886 1700
Size: 110 2889 187 2869
Fragments arranged by size:
2889 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6055
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6055
BssSI C'ACGA_G
Cuts at: 4115 5127 4115
Size: 1012 5043
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2686 794
Size: 1892 4163
BstBI TT'CG_AA
Cuts at: 4859 4859
Size: 6055
BstEII G'GTnAC_C
Cuts at: 1184 2245 1184
Size: 1061 4994
BstYI r'GATC_y
Cuts at: 1972 2608 2856 3024 3218 3385 3752 4137 4154
Size: 636 248 168 194 167 367 385 17
Cuts at: 4154 5606 5692 5704 6050 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 636 385 367 346 248 194
168 167 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2374 513
Size: 552 1309 4194
Fragments arranged by size:
4194 1309 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2422 487
Size: 683 56 1196 4120
Fragments arranged by size:
4120 1196 683 56
BtsI GCAGTG_nn'
Cuts at: 2696 3571 4365 4385 2696
Size: 875 794 20 4366
Fragments arranged by size:
4366 875 794 20
ClaI AT'CG_AT
Cuts at: 1554 2478 1554
Size: 924 5131
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6020
DraI TTT'AAA
Cuts at: 3359 3359
Size: 6055
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5062 809
Size: 507 3746 1802
Fragments arranged by size:
3746 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3502 4391 6020
Size: 54 1425 50 275 18 1672 889 1629
Cuts at: 6020 8
Size: 43
Fragments arranged by size:
1672 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3502 1537
Size: 275 1690 4090
Fragments arranged by size:
4090 1690 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2016 2541 2569 2828 2925 3330 3985 1353
Size: 663 525 28 259 97 405 655 3423
Fragments arranged by size:
3423 663 655 525 405 259 97 28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4642 5016 5162 387
Size: 1064 138 3053 374 146 1280
Fragments arranged by size:
3053 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2324 2345 2633 2721 2927 2993 3068
Size: 393 692 21 288 88 206 66 75
Cuts at: 3068 3116 4118 4715 5502 5770 1239
Size: 48 1002 597 787 268 1524
Fragments arranged by size:
1524 1002 787 692 597 393 288 268
206 88 75 66 48 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2636 2636
Size: 6055
EcoO109I rG'GnC_Cy
Cuts at: 2037 3042 3043 2037
Size: 1005 1 5049
Fragments arranged by size:
5049 1005 1
EcoRI G'AATT_C
Cuts at: 3364 3742 3364
Size: 378 5677
FspI TGC'GCA
Cuts at: 2695 3012 4561 2695
Size: 317 1549 4189
Fragments arranged by size:
4189 1549 317
HaeII r_GCGC'y
Cuts at: 1678 2841 5202 1678
Size: 1163 2361 2531
Fragments arranged by size:
2531 2361 1163
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2026 2058 3016 3048 3139
Size: 32 135 32 454 32 958 32 91
Cuts at: 3139 3171 3203 3235 4696 4728 4770 4802 1373
Size: 32 32 32 1461 32 42 32 2626
Fragments arranged by size:
2626 1461 958 454 135 91 42 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3399 3645 669
Size: 988 281 1461 246 3079
Fragments arranged by size:
3079 1461 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6055
HpaI GTT'AAC
Cuts at: 3645 3645
Size: 6055
KpnI G_GTAC'C
Cuts at: 3380 3380
Size: 6055
MfeI C'AATT_G
Cuts at: 3654 3654
Size: 6055
MluI A'CGCG_T
Cuts at: 5931 5931
Size: 6055
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2851 5169 5353 1305
Size: 1546 2318 184 2007
Fragments arranged by size:
2318 2007 1546 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4233
Fragments arranged by size:
4233 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2544 4013 4372 4531 518
Size: 648 1378 1469 359 159 2042
Fragments arranged by size:
2042 1469 1378 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2498 2699 3016 3058 3471 4148 5296 5541
Size: 892 201 317 42 413 677 1148 245
Cuts at: 5541 1606
Size: 2120
Fragments arranged by size:
2120 1148 892 677 413 317 245 201
42
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5503
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6055
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6055
NotI GC'GGCC_GC
Cuts at: 3502 3502
Size: 6055
NspI r_CATG'y
Cuts at: 1132 1229 2300 2770 3413 4958 1132
Size: 97 1071 470 643 1545 2229
Fragments arranged by size:
2229 1545 1071 643 470 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6055
PciI A'CATG_T
Cuts at: 4954 4954
Size: 6055
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3094 3331 1108
Size: 1986 237 3832
Fragments arranged by size:
3832 1986 237
PfoI T'CCnGG_A
Cuts at: 2030 3111 2030
Size: 1081 4974
PmeI GTTT'AAAC
Cuts at: 3359 3359
Size: 6055
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2807 2839 4124 4156 5637 5669 2807
Size: 32 1285 32 1481 32 3193
Fragments arranged by size:
3193 1481 1285 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2037 2037
Size: 6055
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6055
PsiI TTA'TAA
Cuts at: 3625 3625
Size: 6055
PspOMI G'GGCC_C
Cuts at: 3042 3042
Size: 6055
PstI C_TGCA'G
Cuts at: 830 2475 2691 2850 3407 830
Size: 1645 216 159 557 3478
Fragments arranged by size:
3478 1645 557 216 159
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6055
PvuII CAG'CTG
Cuts at: 2498 2699 3016 3058 2498
Size: 201 317 42 5495
Fragments arranged by size:
5495 317 201 42
SalI G'TCGA_C
Cuts at: 1936 3397 1936
Size: 1461 4594
SapI GCTCTTCn'nnn_
Cuts at: 2541 2828 2541
Size: 287 5768
SbfI CC_TGCA'GG
Cuts at: 3407 3407
Size: 6055
ScaI AGT'ACT
Cuts at: 4303 6015 4303
Size: 1712 4343
SexAI A'CCwGG_T
Cuts at: 3063 3063
Size: 6055
SfcI C'TryA_G
Cuts at: 826 1045 2392 2471 2687 2846 3403 4538 5219
Size: 219 1347 79 216 159 557 1135 681
Cuts at: 5219 5410 826
Size: 191 1471
Fragments arranged by size:
1471 1347 1135 681 557 219 216 191
159 79
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6055
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6055
SmaI CCC'GGG
Cuts at: 1872 2737 3382 1872
Size: 865 645 4545
Fragments arranged by size:
4545 865 645
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2513 3281 4159 5060 5322 820
Size: 197 926 570 768 878 901 262 1553
Fragments arranged by size:
1553 926 901 878 768 570 262 197
SnaBI TAC'GTA
Cuts at: 493 3846 493
Size: 3353 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6055
SphI G_CATG'C
Cuts at: 1132 2300 3413 1132
Size: 1168 1113 3774
Fragments arranged by size:
3774 1168 1113
SspI AAT'ATT
Cuts at: 5 52 3979 5
Size: 47 3927 2081
Fragments arranged by size:
3927 2081 47
StuI AGG'CCT
Cuts at: 1517 2819 1517
Size: 1302 4753
StyI C'CwwG_G
Cuts at: 513 1065 3494 513
Size: 552 2429 3074
Fragments arranged by size:
3074 2429 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3008 3095 4082 4099 4252 4437 4882
Size: 1255 1428 87 987 17 153 185 445
Cuts at: 4882 325
Size: 1498
Fragments arranged by size:
1498 1428 1255 987 445 185 153 87
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4301 6013 96
Size: 274 80 33 51 1443 2324 1712 138
Fragments arranged by size:
2324 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2116 2148 2694 2726 6015 6047 2116
Size: 32 546 32 3289 32 2124
Fragments arranged by size:
3289 2124 546 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6055
XbaI T'CTAG_A
Cuts at: 3391 3391
Size: 6055
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2320 3262 2320
Size: 942 5113
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6055
XmaI C'CCGG_G
Cuts at: 1870 2735 3380 1870
Size: 865 645 4545
Fragments arranged by size:
4545 865 645
XmnI GAAnn'nnTTC
Cuts at: 4184 4184
Size: 6055
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3863 276
Size: 53 83 186 936 2329 2468
Fragments arranged by size:
2468 2329 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspXI PsrI RsrII SacI SacII
SanDI SfiI SfoI SrfI SwaI