Restriction map for
pFN21AE0551
(Circular) (Six-base) MAPSORT of: pFN21AE0551.seq Check: 2433 from: 1 to: 5566
pFN21AE0551 5566 bp
With 182 enzymes: SgfI *
October 20, 2009 10:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3376 278
Size: 53 83 186 936 1840 2468
Fragments arranged by size:
2468 1840 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2187 2205 2909 1937
Size: 250 18 704 4594
Fragments arranged by size:
4594 704 250 18
Acc65I G'GTAC_C
Cuts at: 2887 2887
Size: 5566
AclI AA'CG_TT
Cuts at: 1563 2267 3693 4066 1563
Size: 704 1426 373 3063
Fragments arranged by size:
3063 1426 704 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3629 5013 5281 1632
Size: 1997 1384 268 1917
Fragments arranged by size:
1997 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5369
AflIII A'CryG_T
Cuts at: 1203 2598 2802 4465 5442 1203
Size: 1395 204 1663 977 1327
Fragments arranged by size:
1663 1395 1327 977 204
AgeI A'CCGG_T
Cuts at: 4397 4397
Size: 5566
AhdI GACnn_n'nnGTC
Cuts at: 4295 4295
Size: 5566
AleI CACnn'nnGTG
Cuts at: 1166 2807 1166
Size: 1641 3925
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2357 1184
Size: 1173 4393
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2477 2509 2477
Size: 32 5534
AlwNI CAG_nnn'CTG
Cuts at: 2389 2545 3309 4881 2389
Size: 156 764 1572 3074
Fragments arranged by size:
3074 1572 764 156
ApaI G_GGCC'C
Cuts at: 2023 2023
Size: 5566
ApaLI G'TGCA_C
Cuts at: 3623 4779 3623
Size: 1156 4410
ApoI r'AATT_y
Cuts at: 1493 2875 3101 3253 5362 1493
Size: 1382 226 152 2109 1697
Fragments arranged by size:
2109 1697 1382 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4120 160
Size: 872 3088 1606
Fragments arranged by size:
3088 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5566
AvaI C'yCGr_G
Cuts at: 1870 1943 2891 1870
Size: 73 948 4545
Fragments arranged by size:
4545 948 73
BamHI G'GATC_C
Cuts at: 2437 2896 3263 2437
Size: 459 367 4740
Fragments arranged by size:
4740 459 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2174 2414 2687 2887 4342
Size: 325 217 627 387 240 273 200 1455
Cuts at: 4342 618
Size: 1842
Fragments arranged by size:
1842 1455 627 387 325 273 240 217
200
BanII G_rGCy'C
Cuts at: 1391 2023 2264 2654 2885 1391
Size: 632 241 390 231 4072
Fragments arranged by size:
4072 632 390 241 231
BbeI G_GCGC'C
Cuts at: 2691 2691
Size: 5566
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2787 928
Size: 971 888 3707
Fragments arranged by size:
3707 971 888
BbvCI CC'TCA_GC
Cuts at: 2699 2838 2699
Size: 139 5427
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2454 2488 3757 3791 1903
Size: 34 50 34 433 34 1269 34 3678
Fragments arranged by size:
3678 1269 433 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3458 4668 3458
Size: 1210 4356
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5566
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2027 2611 4177
Size: 107 122 71 631 605 355 584 1566
Cuts at: 4177 136
Size: 1525
Fragments arranged by size:
1566 1525 631 605 584 355 122 107
71
BglII A'GATC_T
Cuts at: 5561 5561
Size: 5566
BlpI GC'TnA_GC
Cuts at: 2983 2983
Size: 5566
Bme1580I G_kGCm'C
Cuts at: 1790 2023 3627 4783 1790
Size: 233 1604 1156 2573
Fragments arranged by size:
2573 1604 1156 233
BmgBI CAC'GTC
Cuts at: 1318 2805 1318
Size: 1487 4079
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2267 3297 4255
Size: 416 53 83 399 24 850 1030 958
Cuts at: 4255 442
Size: 1753
Fragments arranged by size:
1753 1030 958 850 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5566
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2843 4226 1239
Size: 1604 1383 2579
Fragments arranged by size:
2579 1604 1383
Bpu10I CC'TnA_GC
Cuts at: 2699 2838 2699
Size: 139 5427
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3691 4556 4854 3691
Size: 865 298 4403
Fragments arranged by size:
4403 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4229 882
Size: 617 2730 2219
Fragments arranged by size:
2730 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2601 3357 493
Size: 711 1397 756 2702
Fragments arranged by size:
2702 1397 756 711
BsaBI GATnn'nnATC
Cuts at: 2633 5560 2633
Size: 2927 2639
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2688 3373 3755 4390
Size: 53 83 186 936 1155 685 382 635
Cuts at: 4390 275
Size: 1451
Fragments arranged by size:
1451 1155 936 685 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3999 4397 4671 4818 5229 3999
Size: 398 274 147 411 4336
Fragments arranged by size:
4336 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2281 2311 2477 2507 1178
Size: 30 165 30 878 30 166 30 4237
Fragments arranged by size:
4237 878 166 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5566
BseYI C'CCAG_C
Cuts at: 2345 2779 4769 5265 2345
Size: 434 1990 496 2646
Fragments arranged by size:
2646 1990 496 434
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4993
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2595 3016 3777 4805 1540
Size: 275 186 594 421 761 1028 2301
Fragments arranged by size:
2301 1028 761 594 421 275 186
BsiHKAI G_wGCw'C
Cuts at: 3627 3712 4783 3627
Size: 85 1071 4410
Fragments arranged by size:
4410 1071 85
BsmI GAATG_Cn'
Cuts at: 2230 2398 3077 3170 2230
Size: 168 679 93 4626
Fragments arranged by size:
4626 679 168 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2023 2264 2654 2885 3627 3712 4783
Size: 399 233 241 390 231 742 85 1071
Cuts at: 4783 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 390 241 233 231
85
BspEI T'CCGG_A
Cuts at: 5229 5229
Size: 5566
BspHI T'CATG_A
Cuts at: 3455 5185 3455
Size: 1730 3836
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2073 2349 2435 2921 844
Size: 1229 276 86 486 3489
Fragments arranged by size:
3489 1229 486 276 86
BsrBI CCG'CTC
Cuts at: 1394 1823 2014 2619 3453 1394
Size: 429 191 605 834 3507
Fragments arranged by size:
3507 834 605 429 191
BsrDI GCAATG_nn'
Cuts at: 65 1248 2691 4061 4235 65
Size: 1183 1443 1370 174 1396
Fragments arranged by size:
1443 1396 1370 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2610 4210 4397 1700
Size: 110 800 1600 187 2869
Fragments arranged by size:
2869 1600 800 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5566
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5566
BssSI C'ACGA_G
Cuts at: 2180 2705 3626 4638 2180
Size: 525 921 1012 3108
Fragments arranged by size:
3108 1012 921 525
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5566
BstBI TT'CG_AA
Cuts at: 4370 4370
Size: 5566
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5566
BstXI CCAn_nnnn'nTGG
Cuts at: 2527 2779 2527
Size: 252 5314
BstYI r'GATC_y
Cuts at: 1972 2385 2437 2896 3263 3648 3665 5117 5203
Size: 413 52 459 367 385 17 1452 86
Cuts at: 5203 5215 5561 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 459 413 385 367 346 86
52 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2481 2520 513
Size: 552 1416 39 3559
Fragments arranged by size:
3559 1416 552 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4827
Fragments arranged by size:
4827 683 56
BtsI GCAGTG_nn'
Cuts at: 2341 3082 3876 3896 2341
Size: 741 794 20 4011
Fragments arranged by size:
4011 794 741 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5566
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5531
DraI TTT'AAA
Cuts at: 2870 2870
Size: 5566
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2548 4573 809
Size: 507 1232 2025 1802
Fragments arranged by size:
2025 1802 1232 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2079 2603 3013
Size: 54 1425 50 275 18 249 524 410
Cuts at: 3013 3902 5531 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 524 410 275 249 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3013 1537
Size: 275 1201 4090
Fragments arranged by size:
4090 1201 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3496 1353
Size: 2143 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2629 4153 4527 4673 387
Size: 1064 138 1040 1524 374 146 1280
Fragments arranged by size:
1524 1280 1064 1040 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2843 3629 4226 5013 5281 1239
Size: 393 1211 786 597 787 268 1524
Fragments arranged by size:
1524 1211 787 786 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2020 2164 2297 2424 2452 2784 2020
Size: 144 133 127 28 332 4802
Fragments arranged by size:
4802 332 144 133 127 28
EcoRI G'AATT_C
Cuts at: 2875 3253 2875
Size: 378 5188
FspI TGC'GCA
Cuts at: 4072 4072
Size: 5566
HaeII r_GCGC'y
Cuts at: 1678 2691 4713 1678
Size: 1013 2022 2531
Fragments arranged by size:
2531 2022 1013
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2281 2313 2613 2645 4207
Size: 32 135 32 709 32 300 32 1562
Cuts at: 4207 4239 4281 4313 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1562 709 300 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2206 2910 3156 669
Size: 988 281 268 704 246 3079
Fragments arranged by size:
3079 988 704 281 268 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5566
HpaI GTT'AAC
Cuts at: 3156 3156
Size: 5566
KasI G'GCGC_C
Cuts at: 2687 2687
Size: 5566
KpnI G_GTAC'C
Cuts at: 2891 2891
Size: 5566
MfeI C'AATT_G
Cuts at: 3165 3165
Size: 5566
MluI A'CGCG_T
Cuts at: 5442 5442
Size: 5566
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2532 4680 4864 1305
Size: 1227 2148 184 2007
Fragments arranged by size:
2148 2007 1227 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2081 2605 10
Size: 54 1425 343 249 524 2971
Fragments arranged by size:
2971 1425 524 343 249 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2807 3524 3883 4042 518
Size: 648 1641 717 359 159 2042
Fragments arranged by size:
2042 1641 717 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2982 3659 4807 5052 1606
Size: 1376 677 1148 245 2120
Fragments arranged by size:
2120 1376 1148 677 245
NaeI GCC'GGC
Cuts at: 2612 2612
Size: 5566
NarI GG'CG_CC
Cuts at: 2688 2688
Size: 5566
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5014
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5566
NgoMIV G'CCGG_C
Cuts at: 2610 2610
Size: 5566
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5566
NotI GC'GGCC_GC
Cuts at: 3013 3013
Size: 5566
NspI r_CATG'y
Cuts at: 1132 1229 2149 2924 4469 1132
Size: 97 920 775 1545 2229
Fragments arranged by size:
2229 1545 920 775 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5566
PciI A'CATG_T
Cuts at: 4465 4465
Size: 5566
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5566
PfoI T'CCnGG_A
Cuts at: 2219 2859 2219
Size: 640 4926
PmeI GTTT'AAAC
Cuts at: 2870 2870
Size: 5566
PmlI CAC'GTG
Cuts at: 2601 2601
Size: 5566
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2477 2509 3635 3667 5148 5180 2477
Size: 32 1126 32 1481 32 2863
Fragments arranged by size:
2863 1481 1126 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2297 2424 2297
Size: 127 5439
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5566
PsiI TTA'TAA
Cuts at: 3136 3136
Size: 5566
PspOMI G'GGCC_C
Cuts at: 2019 2019
Size: 5566
PstI C_TGCA'G
Cuts at: 830 2070 2336 2918 830
Size: 1240 266 582 3478
Fragments arranged by size:
3478 1240 582 266
PvuI CG_AT'CG
Cuts at: 2001 2595 2001
Size: 594 4972
SalI G'TCGA_C
Cuts at: 1936 2204 2908 1936
Size: 268 704 4594
Fragments arranged by size:
4594 704 268
SbfI CC_TGCA'GG
Cuts at: 2918 2918
Size: 5566
ScaI AGT'ACT
Cuts at: 3814 5526 3814
Size: 1712 3854
SexAI A'CCwGG_T
Cuts at: 2400 2400
Size: 5566
SfcI C'TryA_G
Cuts at: 826 1045 2066 2332 2914 4049 4730 4921 826
Size: 219 1021 266 582 1135 681 191 1471
Fragments arranged by size:
1471 1135 1021 681 582 266 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2027 2027
Size: 5566
SfoI GGC'GCC
Cuts at: 2689 2689
Size: 5566
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5566
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5566
SmaI CCC'GGG
Cuts at: 1872 2893 1872
Size: 1021 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3670 4571 4833 820
Size: 197 926 1727 901 262 1553
Fragments arranged by size:
1727 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3357 493
Size: 2864 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5566
SphI G_CATG'C
Cuts at: 1132 2924 1132
Size: 1792 3774
SspI AAT'ATT
Cuts at: 5 52 3490 5
Size: 47 3438 2081
Fragments arranged by size:
3438 2081 47
StuI AGG'CCT
Cuts at: 1517 2378 1517
Size: 861 4705
StyI C'CwwG_G
Cuts at: 513 1065 2127 2373 3005 513
Size: 552 1062 246 632 3074
Fragments arranged by size:
3074 1062 632 552 246
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2192 2402 3593 3610 3763 3948 4393
Size: 1255 612 210 1191 17 153 185 445
Cuts at: 4393 325
Size: 1498
Fragments arranged by size:
1498 1255 1191 612 445 210 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2487 3812 5524
Size: 274 80 33 51 1443 510 1325 1712
Cuts at: 5524 96
Size: 138
Fragments arranged by size:
1712 1443 1325 510 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5526 5558 5526
Size: 32 5534
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5566
XbaI T'CTAG_A
Cuts at: 2902 2902
Size: 5566
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2125 2254 2125
Size: 129 5437
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5566
XmaI C'CCGG_G
Cuts at: 1870 2891 1870
Size: 1021 4545
XmnI GAAnn'nnTTC
Cuts at: 3695 3695
Size: 5566
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3374 276
Size: 53 83 186 936 1840 2468
Fragments arranged by size:
2468 1840 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SalI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI NruI NsiI PacI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SrfI SwaI