Restriction map for
pFN21AE0550
(Circular) (Six-base) MAPSORT of: pFN21AE0550.seq Check: 8805 from: 1 to: 5626
pFN21AE0550 5626 bp
With 182 enzymes: SgfI *
October 20, 2009 10:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3436 278
Size: 53 83 186 936 1900 2468
Fragments arranged by size:
2468 1900 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2630 2969 1937
Size: 693 339 4594
Fragments arranged by size:
4594 693 339
Acc65I G'GTAC_C
Cuts at: 2947 2947
Size: 5626
AclI AA'CG_TT
Cuts at: 1563 3753 4126 1563
Size: 2190 373 3063
Fragments arranged by size:
3063 2190 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3689 5073 5341 1632
Size: 2057 1384 268 1917
Fragments arranged by size:
2057 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5429
AflIII A'CryG_T
Cuts at: 1203 2019 4525 5502 1203
Size: 816 2506 977 1327
Fragments arranged by size:
2506 1327 977 816
AgeI A'CCGG_T
Cuts at: 4457 4457
Size: 5626
AhdI GACnn_n'nnGTC
Cuts at: 4355 4355
Size: 5626
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5626
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5626
AlwNI CAG_nnn'CTG
Cuts at: 3369 4941 3369
Size: 1572 4054
ApaLI G'TGCA_C
Cuts at: 3683 4839 3683
Size: 1156 4470
ApoI r'AATT_y
Cuts at: 1493 2221 2375 2775 2789 2821 2935 3161 3313
Size: 728 154 400 14 32 114 226 152
Cuts at: 3313 5422 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 728 400 226 154 152 114
32 14
AseI AT'TA_AT
Cuts at: 160 1032 4180 160
Size: 872 3148 1606
Fragments arranged by size:
3148 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5626
AvaI C'yCGr_G
Cuts at: 1870 1943 2951 1870
Size: 73 1008 4545
Fragments arranged by size:
4545 1008 73
BamHI G'GATC_C
Cuts at: 2956 3323 2956
Size: 367 5259
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2947 4402 618
Size: 325 217 627 1160 1455 1842
Fragments arranged by size:
1842 1455 1160 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2393 2736 2945 1391
Size: 1002 343 209 4072
Fragments arranged by size:
4072 1002 343 209
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2038 928
Size: 971 139 4516
Fragments arranged by size:
4516 971 139
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2857 2891 3817 3851 1903
Size: 34 920 34 926 34 3678
Fragments arranged by size:
3678 926 920 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3518 4728 3518
Size: 1210 4416
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5626
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4237 136
Size: 107 122 71 631 605 2565 1525
Fragments arranged by size:
2565 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2499 5621 2499
Size: 3122 2504
BlpI GC'TnA_GC
Cuts at: 3043 3043
Size: 5626
Bme1580I G_kGCm'C
Cuts at: 1790 3687 4843 1790
Size: 1897 1156 2573
Fragments arranged by size:
2573 1897 1156
BmgBI CAC'GTC
Cuts at: 1318 2828 1318
Size: 1510 4116
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3357 4315 442
Size: 416 53 83 399 24 1940 958 1753
Fragments arranged by size:
1940 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2099 1056
Size: 1043 4583
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2273 2685 2778 2883 2907 4286 1239
Size: 1034 412 93 105 24 1379 2579
Fragments arranged by size:
2579 1379 1034 412 105 93 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3751 4616 4914 3751
Size: 865 298 4463
Fragments arranged by size:
4463 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2516 4289 882
Size: 617 1017 1773 2219
Fragments arranged by size:
2219 1773 1017 617
BsaAI yAC'GTr
Cuts at: 493 1204 3417 493
Size: 711 2213 2702
Fragments arranged by size:
2702 2213 711
BsaBI GATnn'nnATC
Cuts at: 2480 5620 2480
Size: 3140 2486
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3433 3815 4450 275
Size: 53 83 186 936 1900 382 635 1451
Fragments arranged by size:
1900 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4059 4457 4731 4878 5289 4059
Size: 398 274 147 411 4396
Fragments arranged by size:
4396 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2045 2075 1178
Size: 30 165 30 642 30 4729
Fragments arranged by size:
4729 642 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2639 1186
Size: 1453 4173
BseYI C'CCAG_C
Cuts at: 2671 2735 4829 5325 2671
Size: 64 2094 496 2972
Fragments arranged by size:
2972 2094 496 64
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5053
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3076 3837 4865 1540
Size: 275 186 1075 761 1028 2301
Fragments arranged by size:
2301 1075 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3687 3772 4843 3687
Size: 85 1071 4470
Fragments arranged by size:
4470 1071 85
BsmI GAATG_Cn'
Cuts at: 3137 3230 3137
Size: 93 5533
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2393 2736 2945 3687 3772 4843 1391
Size: 399 603 343 209 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 603 399 343 209 85
BspEI T'CCGG_A
Cuts at: 5289 5289
Size: 5626
BspHI T'CATG_A
Cuts at: 3515 5245 3515
Size: 1730 3896
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2981 844
Size: 2137 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3513 1394
Size: 429 1690 3507
Fragments arranged by size:
3507 1690 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2900 4121 4295 65
Size: 1183 1652 1221 174 1396
Fragments arranged by size:
1652 1396 1221 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4270 4457 1700
Size: 110 2460 187 2869
Fragments arranged by size:
2869 2460 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5626
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5626
BssSI C'ACGA_G
Cuts at: 3686 4698 3686
Size: 1012 4614
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5626
BstBI TT'CG_AA
Cuts at: 4430 4430
Size: 5626
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5626
BstYI r'GATC_y
Cuts at: 1972 2499 2693 2956 3323 3708 3725 5177 5263
Size: 527 194 263 367 385 17 1452 86
Cuts at: 5263 5275 5621 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 527 385 367 346 263 194
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5074
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4887
Fragments arranged by size:
4887 683 56
BtsI GCAGTG_nn'
Cuts at: 2547 3142 3936 3956 2547
Size: 595 794 20 4217
Fragments arranged by size:
4217 794 595 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5626
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5591
DraI TTT'AAA
Cuts at: 2794 2930 2794
Size: 136 5490
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4633 809
Size: 507 3317 1802
Fragments arranged by size:
3317 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2361 3073 3962
Size: 54 1425 50 275 18 531 712 889
Cuts at: 3962 5591 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 712 531 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3073 1537
Size: 275 1261 4090
Fragments arranged by size:
4090 1261 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2844 3556 1353
Size: 1491 712 3423
Fragments arranged by size:
3423 1491 712
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4213 4587 4733 387
Size: 1064 138 2624 374 146 1280
Fragments arranged by size:
2624 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2273 2685 2778 2883 2907 3689 4286
Size: 393 641 412 93 105 24 782 597
Cuts at: 4286 5073 5341 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 782 641 597 412 393 268
105 93 24
EcoNI CCTnn'n_nnAGG
Cuts at: 2333 2333
Size: 5626
EcoO109I rG'GnC_Cy
Cuts at: 2102 2102
Size: 5626
EcoRI G'AATT_C
Cuts at: 2221 2775 2935 3313 2221
Size: 554 160 378 4534
Fragments arranged by size:
4534 554 378 160
FspI TGC'GCA
Cuts at: 4132 4132
Size: 5626
HaeII r_GCGC'y
Cuts at: 1678 4773 1678
Size: 3095 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4267 4299 4341 4373 1373
Size: 32 135 32 2695 32 42 32 2626
Fragments arranged by size:
2695 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2350 2631 2970 3216 669
Size: 988 281 412 281 339 246 3079
Fragments arranged by size:
3079 988 412 339 281 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5626
HpaI GTT'AAC
Cuts at: 3216 3216
Size: 5626
KpnI G_GTAC'C
Cuts at: 2951 2951
Size: 5626
MfeI C'AATT_G
Cuts at: 3225 3225
Size: 5626
MluI A'CGCG_T
Cuts at: 5502 5502
Size: 5626
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2404 4740 4924 1305
Size: 1099 2336 184 2007
Fragments arranged by size:
2336 2007 1099 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2363 10
Size: 54 1425 343 531 3273
Fragments arranged by size:
3273 1425 531 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2567 3584 3943 4102 518
Size: 648 1401 1017 359 159 2042
Fragments arranged by size:
2042 1401 1017 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2076 3042 3719 4867 5112 1606
Size: 470 966 677 1148 245 2120
Fragments arranged by size:
2120 1148 966 677 470 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5074
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5626
NheI G'CTAG_C
Cuts at: 1052 2095 1052
Size: 1043 4583
NotI GC'GGCC_GC
Cuts at: 3073 3073
Size: 5626
NspI r_CATG'y
Cuts at: 1132 1229 2023 2984 4529 1132
Size: 97 794 961 1545 2229
Fragments arranged by size:
2229 1545 961 794 97
PasI CC'CwG_GG
Cuts at: 1348 2040 2587 1348
Size: 692 547 4387
Fragments arranged by size:
4387 692 547
PciI A'CATG_T
Cuts at: 2019 4525 2019
Size: 2506 3120
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5626
PfoI T'CCnGG_A
Cuts at: 2400 2400
Size: 5626
PmeI GTTT'AAAC
Cuts at: 2930 2930
Size: 5626
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3695 3727 5208 5240 3695
Size: 32 1481 32 4081
Fragments arranged by size:
4081 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5626
PsiI TTA'TAA
Cuts at: 3196 3196
Size: 5626
PstI C_TGCA'G
Cuts at: 830 2978 830
Size: 2148 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5626
RsrII CG'GwC_CG
Cuts at: 2227 2227
Size: 5626
SalI G'TCGA_C
Cuts at: 1936 2629 2968 1936
Size: 693 339 4594
Fragments arranged by size:
4594 693 339
SbfI CC_TGCA'GG
Cuts at: 2978 2978
Size: 5626
ScaI AGT'ACT
Cuts at: 3874 5586 3874
Size: 1712 3914
SfcI C'TryA_G
Cuts at: 826 1045 2974 4109 4790 4981 826
Size: 219 1929 1135 681 191 1471
Fragments arranged by size:
1929 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5626
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5626
SmaI CCC'GGG
Cuts at: 1872 2953 1872
Size: 1081 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3730 4631 4893 820
Size: 197 926 1787 901 262 1553
Fragments arranged by size:
1787 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3417 493
Size: 2924 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5626
SphI G_CATG'C
Cuts at: 1132 2984 1132
Size: 1852 3774
SspI AAT'ATT
Cuts at: 5 52 3550 5
Size: 47 3498 2081
Fragments arranged by size:
3498 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5626
StyI C'CwwG_G
Cuts at: 513 1065 3065 513
Size: 552 2000 3074
Fragments arranged by size:
3074 2000 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3653 3670 3823 4008 4453 325
Size: 1255 2073 17 153 185 445 1498
Fragments arranged by size:
2073 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3872 5584 96
Size: 274 80 33 51 1443 1895 1712 138
Fragments arranged by size:
1895 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5586 5618 5586
Size: 32 5594
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5626
XbaI T'CTAG_A
Cuts at: 2962 2962
Size: 5626
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5626
XmaI C'CCGG_G
Cuts at: 1870 2951 1870
Size: 1081 4545
XmnI GAAnn'nnTTC
Cuts at: 3755 3755
Size: 5626
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3434 276
Size: 53 83 186 936 1900 2468
Fragments arranged by size:
2468 1900 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI RsrII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspOMI PspXI PsrI PvuII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI