Restriction map for
pFN21AE0546
(Circular) (Six-base) MAPSORT of: pFN21AE0546.seq Check: 5636 from: 1 to: 5596
pFN21AE0546 5596 bp
With 182 enzymes: SgfI *
October 20, 2009 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2494 2494
Size: 5596
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3406 278
Size: 53 83 186 936 1870 2468
Fragments arranged by size:
2468 1870 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2939 1937
Size: 1002 4594
Acc65I G'GTAC_C
Cuts at: 2917 2917
Size: 5596
AclI AA'CG_TT
Cuts at: 1563 3723 4096 1563
Size: 2160 373 3063
Fragments arranged by size:
3063 2160 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2429 3659 5043 5311 1632
Size: 797 1230 1384 268 1917
Fragments arranged by size:
1917 1384 1230 797 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5399
AflIII A'CryG_T
Cuts at: 1203 2103 4495 5472 1203
Size: 900 2392 977 1327
Fragments arranged by size:
2392 1327 977 900
AgeI A'CCGG_T
Cuts at: 4427 4427
Size: 5596
AhdI GACnn_n'nnGTC
Cuts at: 2206 4325 2206
Size: 2119 3477
AleI CACnn'nnGTG
Cuts at: 1166 2288 1166
Size: 1122 4474
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5596
AlwNI CAG_nnn'CTG
Cuts at: 2509 3339 4911 2509
Size: 830 1572 3194
Fragments arranged by size:
3194 1572 830
ApaI G_GGCC'C
Cuts at: 2085 2681 2854 2085
Size: 596 173 4827
Fragments arranged by size:
4827 596 173
ApaLI G'TGCA_C
Cuts at: 3653 4809 3653
Size: 1156 4440
ApoI r'AATT_y
Cuts at: 1493 2905 3131 3283 5392 1493
Size: 1412 226 152 2109 1697
Fragments arranged by size:
2109 1697 1412 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4150 160
Size: 872 3118 1606
Fragments arranged by size:
3118 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5596
AvaI C'yCGr_G
Cuts at: 1870 1943 2127 2150 2334 2608 2921 1870
Size: 73 184 23 184 274 313 4545
Fragments arranged by size:
4545 313 274 184 184 73 23
BamHI G'GATC_C
Cuts at: 2926 3293 2926
Size: 367 5229
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2123 2168 2573 2624 2917
Size: 325 217 627 336 45 405 51 293
Cuts at: 2917 4372 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 405 336 325 293 217
51 45
BanII G_rGCy'C
Cuts at: 1391 2085 2439 2681 2691 2854 2915 1391
Size: 694 354 242 10 163 61 4072
Fragments arranged by size:
4072 694 354 242 163 61 10
BbeI G_GCGC'C
Cuts at: 2172 2172
Size: 5596
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2452 928
Size: 971 553 4072
Fragments arranged by size:
4072 971 553
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3787 3821 1903
Size: 34 1850 34 3678
Fragments arranged by size:
3678 1850 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3488 4698 3488
Size: 1210 4386
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5596
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2268 4207 136
Size: 107 122 71 631 605 596 1939 1525
Fragments arranged by size:
1939 1525 631 605 596 122 107 71
BglII A'GATC_T
Cuts at: 5591 5591
Size: 5596
BlpI GC'TnA_GC
Cuts at: 3013 3013
Size: 5596
Bme1580I G_kGCm'C
Cuts at: 1790 2085 2576 2681 2854 3657 4813 1790
Size: 295 491 105 173 803 1156 2573
Fragments arranged by size:
2573 1156 803 491 295 173 105
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5596
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3327 4285 442
Size: 416 53 83 399 24 1910 958 1753
Fragments arranged by size:
1910 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5596
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2531 4256 1239
Size: 1292 1725 2579
Fragments arranged by size:
2579 1725 1292
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2474 2541 3721 4586 4884 2474
Size: 67 1180 865 298 3186
Fragments arranged by size:
3186 1180 865 298 67
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2041 2559 4259 882
Size: 617 542 518 1700 2219
Fragments arranged by size:
2219 1700 617 542 518
BsaAI yAC'GTr
Cuts at: 493 1204 2161 2290 2745 2773 3387 493
Size: 711 957 129 455 28 614 2702
Fragments arranged by size:
2702 957 711 614 455 129 28
BsaBI GATnn'nnATC
Cuts at: 5590 5590
Size: 5596
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2169 2207 3403 3785
Size: 53 83 186 936 636 38 1196 382
Cuts at: 3785 4420 275
Size: 635 1451
Fragments arranged by size:
1451 1196 936 636 635 382 186 83
53 38
BsaWI w'CCGG_w
Cuts at: 2397 4029 4427 4701 4848 5259 2397
Size: 1632 398 274 147 411 2734
Fragments arranged by size:
2734 1632 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2810 2840 1178
Size: 30 165 30 1407 30 3934
Fragments arranged by size:
3934 1407 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5596
BseYI C'CCAG_C
Cuts at: 2077 2231 4799 5295 2077
Size: 154 2568 496 2378
Fragments arranged by size:
2568 2378 496 154
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5023
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2457 3046 3807 4835 1540
Size: 275 186 456 589 761 1028 2301
Fragments arranged by size:
2301 1028 761 589 456 275 186
BsiHKAI G_wGCw'C
Cuts at: 2022 2385 2691 2698 3657 3742 4813 2022
Size: 363 306 7 959 85 1071 2805
Fragments arranged by size:
2805 1071 959 363 306 85 7
BsmI GAATG_Cn'
Cuts at: 3107 3200 3107
Size: 93 5503
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2022 2085 2385 2439 2576 2681 2691
Size: 399 232 63 300 54 137 105 10
Cuts at: 2691 2698 2854 2915 3657 3742 4813 1391
Size: 7 156 61 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 300 232 156 137
105 85 63 61 54 10 7
BspEI T'CCGG_A
Cuts at: 2397 5259 2397
Size: 2862 2734
BspHI T'CATG_A
Cuts at: 3485 5215 3485
Size: 1730 3866
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2494 2951 844
Size: 1650 457 3489
Fragments arranged by size:
3489 1650 457
BsrBI CCG'CTC
Cuts at: 1394 1823 2481 3483 1394
Size: 429 658 1002 3507
Fragments arranged by size:
3507 1002 658 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2736 4091 4265 65
Size: 1183 1488 1355 174 1396
Fragments arranged by size:
1488 1396 1355 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2452 4240 4427 1700
Size: 110 642 1788 187 2869
Fragments arranged by size:
2869 1788 642 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5596
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5596
BssSI C'ACGA_G
Cuts at: 3656 4668 3656
Size: 1012 4584
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5596
BstBI TT'CG_AA
Cuts at: 4400 4400
Size: 5596
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5596
BstXI CCAn_nnnn'nTGG
Cuts at: 2050 2621 2716 2050
Size: 571 95 4930
Fragments arranged by size:
4930 571 95
BstYI r'GATC_y
Cuts at: 1972 2719 2926 3293 3678 3695 5147 5233 5245
Size: 747 207 367 385 17 1452 86 12
Cuts at: 5245 5591 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 747 385 367 346 207 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2620 513
Size: 552 939 616 3489
Fragments arranged by size:
3489 939 616 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4857
Fragments arranged by size:
4857 683 56
BtsI GCAGTG_nn'
Cuts at: 3112 3906 3926 3112
Size: 794 20 4782
Fragments arranged by size:
4782 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5596
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5561
DraI TTT'AAA
Cuts at: 2900 2900
Size: 5596
DraIII CAC_nnn'GTG
Cuts at: 2046 2293 2425 2046
Size: 247 132 5217
Fragments arranged by size:
5217 247 132
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4603 809
Size: 507 3287 1802
Fragments arranged by size:
3287 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2362 2454 2811
Size: 54 1425 50 275 18 532 92 357
Cuts at: 2811 3043 3932 5561 8
Size: 232 889 1629 43
Fragments arranged by size:
1629 1425 889 532 357 275 232 92
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2454 3043 1537
Size: 275 642 589 4090
Fragments arranged by size:
4090 642 589 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2184 2679 3526 1353
Size: 831 495 847 3423
Fragments arranged by size:
3423 847 831 495
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2069 4183 4557 4703 387
Size: 1064 138 480 2114 374 146 1280
Fragments arranged by size:
2114 1280 1064 480 374 146 138
EcoICRI GAG'CTC
Cuts at: 2689 2689
Size: 5596
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2429 2531 3659 4256 5043 5311 1239
Size: 393 797 102 1128 597 787 268 1524
Fragments arranged by size:
1524 1128 797 787 597 393 268 102
EcoNI CCTnn'n_nnAGG
Cuts at: 2657 2657
Size: 5596
EcoO109I rG'GnC_Cy
Cuts at: 2081 2677 2851 2081
Size: 596 174 4826
Fragments arranged by size:
4826 596 174
EcoRI G'AATT_C
Cuts at: 2905 3283 2905
Size: 378 5218
FspI TGC'GCA
Cuts at: 4102 4102
Size: 5596
HaeII r_GCGC'y
Cuts at: 1678 2033 2158 2172 4743 1678
Size: 355 125 14 2571 2531
Fragments arranged by size:
2571 2531 355 125 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2192 2224 2537 2569 4237
Size: 32 135 32 620 32 313 32 1668
Cuts at: 4237 4269 4311 4343 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1668 620 313 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2632 2940 3186 669
Size: 988 281 694 308 246 3079
Fragments arranged by size:
3079 988 694 308 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5596
HpaI GTT'AAC
Cuts at: 3186 3186
Size: 5596
KasI G'GCGC_C
Cuts at: 2168 2168
Size: 5596
KpnI G_GTAC'C
Cuts at: 2921 2921
Size: 5596
MfeI C'AATT_G
Cuts at: 3195 3195
Size: 5596
MluI A'CGCG_T
Cuts at: 2103 5472 2103
Size: 3369 2227
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4710 4894 1305
Size: 3405 184 2007
Fragments arranged by size:
3405 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2364 10
Size: 54 1425 343 532 3242
Fragments arranged by size:
3242 1425 532 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2288 2619 3554 3913 4072 518
Size: 648 1122 331 935 359 159 2042
Fragments arranged by size:
2042 1122 935 648 359 331 159
MspA1I CmG'CkG
Cuts at: 1606 2539 3012 3689 4837 5082 1606
Size: 933 473 677 1148 245 2120
Fragments arranged by size:
2120 1148 933 677 473 245
NarI GG'CG_CC
Cuts at: 2169 2169
Size: 5596
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4105
Fragments arranged by size:
4105 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5596
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5596
NotI GC'GGCC_GC
Cuts at: 3043 3043
Size: 5596
NspI r_CATG'y
Cuts at: 1132 1229 2638 2954 4499 1132
Size: 97 1409 316 1545 2229
Fragments arranged by size:
2229 1545 1409 316 97
PasI CC'CwG_GG
Cuts at: 1348 2875 1348
Size: 1527 4069
PciI A'CATG_T
Cuts at: 4495 4495
Size: 5596
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2046 2542 1108
Size: 938 496 4162
Fragments arranged by size:
4162 938 496
PmeI GTTT'AAAC
Cuts at: 2900 2900
Size: 5596
PmlI CAC'GTG
Cuts at: 2290 2745 2773 2290
Size: 455 28 5113
Fragments arranged by size:
5113 455 28
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3665 3697 5178 5210 3665
Size: 32 1481 32 4051
Fragments arranged by size:
4051 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5596
PsiI TTA'TAA
Cuts at: 3166 3166
Size: 5596
PspOMI G'GGCC_C
Cuts at: 2081 2677 2850 2081
Size: 596 173 4827
Fragments arranged by size:
4827 596 173
PspXI vC'TCGA_Gb
Cuts at: 2150 2150
Size: 5596
PstI C_TGCA'G
Cuts at: 830 2948 830
Size: 2118 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5596
PvuII CAG'CTG
Cuts at: 2539 2539
Size: 5596
SacI G_AGCT'C
Cuts at: 2691 2691
Size: 5596
SalI G'TCGA_C
Cuts at: 1936 2938 1936
Size: 1002 4594
SapI GCTCTTCn'nnn_
Cuts at: 2679 2679
Size: 5596
SbfI CC_TGCA'GG
Cuts at: 2948 2948
Size: 5596
ScaI AGT'ACT
Cuts at: 3844 5556 3844
Size: 1712 3884
SfcI C'TryA_G
Cuts at: 826 1045 2944 4079 4760 4951 826
Size: 219 1899 1135 681 191 1471
Fragments arranged by size:
1899 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2170 2170
Size: 5596
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5596
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5596
SmaI CCC'GGG
Cuts at: 1872 2336 2923 1872
Size: 464 587 4545
Fragments arranged by size:
4545 587 464
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2150 2489 2556 3700 4601 4863
Size: 197 926 207 339 67 1144 901 262
Cuts at: 4863 820
Size: 1553
Fragments arranged by size:
1553 1144 926 901 339 262 207 197
67
SnaBI TAC'GTA
Cuts at: 493 3387 493
Size: 2894 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5596
SphI G_CATG'C
Cuts at: 1132 2954 1132
Size: 1822 3774
SspI AAT'ATT
Cuts at: 5 52 3520 5
Size: 47 3468 2081
Fragments arranged by size:
3468 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5596
StyI C'CwwG_G
Cuts at: 513 1065 2004 2778 3035 513
Size: 552 939 774 257 3074
Fragments arranged by size:
3074 939 774 552 257
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3623 3640 3793 3978 4423 325
Size: 1255 2043 17 153 185 445 1498
Fragments arranged by size:
2043 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3842 5554 96
Size: 274 80 33 51 1443 1865 1712 138
Fragments arranged by size:
1865 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2338 2370 5556 5588 2338
Size: 32 3186 32 2346
Fragments arranged by size:
3186 2346 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5596
XbaI T'CTAG_A
Cuts at: 2932 2932
Size: 5596
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2047 2047
Size: 5596
XhoI C'TCGA_G
Cuts at: 1943 2150 1943
Size: 207 5389
XmaI C'CCGG_G
Cuts at: 1870 2334 2921 1870
Size: 464 587 4545
Fragments arranged by size:
4545 587 464
XmnI GAAnn'nnTTC
Cuts at: 3725 3725
Size: 5596
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3404 276
Size: 53 83 186 936 1870 2468
Fragments arranged by size:
2468 1870 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PfoI PpuMI
PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI