Restriction map for
pFN21AE0545
(Circular) (Six-base) MAPSORT of: pFN21AE0545.seq Check: 2420 from: 1 to: 5836
pFN21AE0545 5836 bp
With 182 enzymes: SgfI *
October 20, 2009 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2074 2074
Size: 5836
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3646 278
Size: 53 83 186 936 2110 2468
Fragments arranged by size:
2468 2110 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2505 3179 1937
Size: 568 674 4594
Fragments arranged by size:
4594 674 568
Acc65I G'GTAC_C
Cuts at: 3157 3157
Size: 5836
AclI AA'CG_TT
Cuts at: 1563 3963 4336 1563
Size: 2400 373 3063
Fragments arranged by size:
3063 2400 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3899 5283 5551 1632
Size: 2267 1384 268 1917
Fragments arranged by size:
2267 1917 1384 268
AfeI AGC'GCT
Cuts at: 2895 2895
Size: 5836
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5639
AflIII A'CryG_T
Cuts at: 1203 4735 5712 1203
Size: 3532 977 1327
Fragments arranged by size:
3532 1327 977
AgeI A'CCGG_T
Cuts at: 4667 4667
Size: 5836
AhdI GACnn_n'nnGTC
Cuts at: 4565 4565
Size: 5836
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5836
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2059 2222 1184
Size: 875 163 4798
Fragments arranged by size:
4798 875 163
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3098 3130 3098
Size: 32 5804
AlwNI CAG_nnn'CTG
Cuts at: 3579 5151 3579
Size: 1572 4264
ApaLI G'TGCA_C
Cuts at: 2570 3893 5049 2570
Size: 1323 1156 3357
Fragments arranged by size:
3357 1323 1156
ApoI r'AATT_y
Cuts at: 1493 3145 3371 3523 5632 1493
Size: 1652 226 152 2109 1697
Fragments arranged by size:
2109 1697 1652 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4390 160
Size: 872 3358 1606
Fragments arranged by size:
3358 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5836
AvaI C'yCGr_G
Cuts at: 1870 1943 2127 2279 3161 1870
Size: 73 184 152 882 4545
Fragments arranged by size:
4545 882 184 152 73
BamHI G'GATC_C
Cuts at: 3166 3533 3166
Size: 367 5469
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2171 2243 3157 4612 618
Size: 325 217 627 384 72 914 1455 1842
Fragments arranged by size:
1842 1455 914 627 384 325 217 72
BanII G_rGCy'C
Cuts at: 1391 2126 3155 1391
Size: 735 1029 4072
Fragments arranged by size:
4072 1029 735
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2814 928
Size: 971 915 3950
Fragments arranged by size:
3950 971 915
BbvCI CC'TCA_GC
Cuts at: 2615 2615
Size: 5836
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4027 4061 1903
Size: 34 50 34 2006 34 3678
Fragments arranged by size:
3678 2006 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3728 4938 3728
Size: 1210 4626
BclI T'GATC_A
Cuts at: 1548 2580 1548
Size: 1032 4804
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2417 4447 136
Size: 107 122 71 631 605 745 2030 1525
Fragments arranged by size:
2030 1525 745 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5831 5831
Size: 5836
BlpI GC'TnA_GC
Cuts at: 2967 3253 2967
Size: 286 5550
Bme1580I G_kGCm'C
Cuts at: 1790 2174 2574 3897 5053 1790
Size: 384 400 1323 1156 2573
Fragments arranged by size:
2573 1323 1156 400 384
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5836
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2668 3567 4525
Size: 416 53 83 399 24 1251 899 958
Cuts at: 4525 442
Size: 1753
Fragments arranged by size:
1753 1251 958 899 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5836
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2450 2803 4496 1239
Size: 1211 353 1693 2579
Fragments arranged by size:
2579 1693 1211 353
Bpu10I CC'TnA_GC
Cuts at: 2145 2615 2145
Size: 470 5366
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2477 2735 3961 4826 5124 2477
Size: 258 1226 865 298 3189
Fragments arranged by size:
3189 1226 865 298 258
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2294 4499 882
Size: 617 795 2205 2219
Fragments arranged by size:
2219 2205 795 617
BsaAI yAC'GTr
Cuts at: 493 1204 2182 3627 493
Size: 711 978 1445 2702
Fragments arranged by size:
2702 1445 978 711
BsaBI GATnn'nnATC
Cuts at: 5830 5830
Size: 5836
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2271 2406 3643 4025
Size: 53 83 186 936 738 135 1237 382
Cuts at: 4025 4660 275
Size: 635 1451
Fragments arranged by size:
1451 1237 936 738 635 382 186 135
83 53
BsaWI w'CCGG_w
Cuts at: 4269 4667 4941 5088 5499 4269
Size: 398 274 147 411 4606
Fragments arranged by size:
4606 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3098 3128 1178
Size: 30 165 30 1695 30 3886
Fragments arranged by size:
3886 1695 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2168 2312 3106 1186
Size: 982 144 794 3916
Fragments arranged by size:
3916 982 794 144
BseYI C'CCAG_C
Cuts at: 2074 5039 5535 2074
Size: 2965 496 2375
Fragments arranged by size:
2965 2375 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2717 1163
Size: 573 981 4282
Fragments arranged by size:
4282 981 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2560 3286 4047 5075 1540
Size: 275 186 559 726 761 1028 2301
Fragments arranged by size:
2301 1028 761 726 559 275 186
BsiHKAI G_wGCw'C
Cuts at: 2469 2574 3897 3982 5053 2469
Size: 105 1323 85 1071 3252
Fragments arranged by size:
3252 1323 1071 105 85
BsmI GAATG_Cn'
Cuts at: 3347 3440 3347
Size: 93 5743
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2126 2174 2469 2574 3155 3897 3982
Size: 399 336 48 295 105 581 742 85
Cuts at: 3982 5053 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 581 399 336 295 105
85 48
BspEI T'CCGG_A
Cuts at: 5499 5499
Size: 5836
BspHI T'CATG_A
Cuts at: 3725 5455 3725
Size: 1730 4106
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2074 3191 844
Size: 1230 1117 3489
Fragments arranged by size:
3489 1230 1117
BsrBI CCG'CTC
Cuts at: 1394 1823 2212 2854 3723 1394
Size: 429 389 642 869 3507
Fragments arranged by size:
3507 869 642 429 389
BsrDI GCAATG_nn'
Cuts at: 65 1248 2598 4331 4505 65
Size: 1183 1350 1733 174 1396
Fragments arranged by size:
1733 1396 1350 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2039 2455 4480 4667 1700
Size: 110 229 416 2025 187 2869
Fragments arranged by size:
2869 2025 416 229 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5836
BssHII G'CGCG_C
Cuts at: 1925 2257 1925
Size: 332 5504
BssSI C'ACGA_G
Cuts at: 3896 4908 3896
Size: 1012 4824
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2728 794
Size: 1934 3902
BstBI TT'CG_AA
Cuts at: 4640 4640
Size: 5836
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5836
BstXI CCAn_nnnn'nTGG
Cuts at: 2182 2921 2182
Size: 739 5097
BstYI r'GATC_y
Cuts at: 1972 3166 3533 3918 3935 5387 5473 5485 5831
Size: 1194 367 385 17 1452 86 12 346
Cuts at: 5831 1972
Size: 1977
Fragments arranged by size:
1977 1452 1194 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2859 513
Size: 552 1794 3490
Fragments arranged by size:
3490 1794 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2761 487
Size: 683 56 1535 3562
Fragments arranged by size:
3562 1535 683 56
BtsI GCAGTG_nn'
Cuts at: 2245 3352 4146 4166 2245
Size: 1107 794 20 3915
Fragments arranged by size:
3915 1107 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5836
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2809 2844 529
Size: 35 2245 35 3521
Fragments arranged by size:
3521 2245 35 35
DraI TTT'AAA
Cuts at: 3140 3140
Size: 5836
DraIII CAC_nnn'GTG
Cuts at: 2643 2643
Size: 5836
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3023 4843 809
Size: 507 1707 1820 1802
Fragments arranged by size:
1820 1802 1707 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2325 2353 2557
Size: 54 1425 50 275 18 495 28 204
Cuts at: 2557 2911 3283 4172 5801 8
Size: 354 372 889 1629 43
Fragments arranged by size:
1629 1425 889 495 372 354 275 204
54 50 43 28 18
EagI C'GGCC_G
Cuts at: 1537 1812 2557 3283 1537
Size: 275 745 726 4090
Fragments arranged by size:
4090 745 726 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3766 1353
Size: 2413 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4423 4797 4943 387
Size: 1064 138 2834 374 146 1280
Fragments arranged by size:
2834 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2450 2803 3899 4496 5283 5551 1239
Size: 393 818 353 1096 597 787 268 1524
Fragments arranged by size:
1524 1096 818 787 597 393 353 268
EcoO109I rG'GnC_Cy
Cuts at: 2479 2479
Size: 5836
EcoRI G'AATT_C
Cuts at: 3145 3523 3145
Size: 378 5458
FspI TGC'GCA
Cuts at: 4342 4342
Size: 5836
HaeII r_GCGC'y
Cuts at: 1678 2746 2897 4983 1678
Size: 1068 151 2086 2531
Fragments arranged by size:
2531 2086 1068 151
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4477 4509 4551 4583 1373
Size: 32 135 32 2905 32 42 32 2626
Fragments arranged by size:
2905 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2884 3180 3426 669
Size: 988 281 946 296 246 3079
Fragments arranged by size:
3079 988 946 296 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5836
HpaI GTT'AAC
Cuts at: 2884 3426 2884
Size: 542 5294
KpnI G_GTAC'C
Cuts at: 3161 3161
Size: 5836
MfeI C'AATT_G
Cuts at: 3435 3435
Size: 5836
MluI A'CGCG_T
Cuts at: 5712 5712
Size: 5836
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4950 5134 1305
Size: 3645 184 2007
Fragments arranged by size:
3645 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2327 2913 10
Size: 54 1425 343 495 586 2933
Fragments arranged by size:
2933 1425 586 495 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2219 2252 2660 3794 4153 4312 518
Size: 648 1053 33 408 1134 359 159 2042
Fragments arranged by size:
2042 1134 1053 648 408 359 159 33
MspA1I CmG'CkG
Cuts at: 1606 2074 2725 3043 3252 3929 5077 5322 1606
Size: 468 651 318 209 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 651 468 318 245 209
NaeI GCC'GGC
Cuts at: 2041 2457 2041
Size: 416 5420
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5284
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5836
NgoMIV G'CCGG_C
Cuts at: 2039 2455 2039
Size: 416 5420
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5836
NotI GC'GGCC_GC
Cuts at: 3283 3283
Size: 5836
NspI r_CATG'y
Cuts at: 1132 1229 3194 4739 1132
Size: 97 1965 1545 2229
Fragments arranged by size:
2229 1965 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2921 1348
Size: 1573 4263
PciI A'CATG_T
Cuts at: 4735 4735
Size: 5836
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2680 1108
Size: 1572 4264
PfoI T'CCnGG_A
Cuts at: 2427 2427
Size: 5836
PmeI GTTT'AAAC
Cuts at: 3140 3140
Size: 5836
PmlI CAC'GTG
Cuts at: 2182 2182
Size: 5836
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3098 3130 3905 3937 5418 5450 3098
Size: 32 775 32 1481 32 3484
Fragments arranged by size:
3484 1481 775 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2479 2479
Size: 5836
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5836
PsiI TTA'TAA
Cuts at: 3406 3406
Size: 5836
PstI C_TGCA'G
Cuts at: 830 3188 830
Size: 2358 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5836
PvuII CAG'CTG
Cuts at: 2074 2725 2074
Size: 651 5185
SalI G'TCGA_C
Cuts at: 1936 3178 1936
Size: 1242 4594
SbfI CC_TGCA'GG
Cuts at: 3188 3188
Size: 5836
ScaI AGT'ACT
Cuts at: 4084 5796 4084
Size: 1712 4124
SexAI A'CCwGG_T
Cuts at: 2865 2865
Size: 5836
SfcI C'TryA_G
Cuts at: 826 1045 3184 4319 5000 5191 826
Size: 219 2139 1135 681 191 1471
Fragments arranged by size:
2139 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5836
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5836
SmaI CCC'GGG
Cuts at: 1872 2129 3163 1872
Size: 257 1034 4545
Fragments arranged by size:
4545 1034 257
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2492 2750 3940 4841 5103 820
Size: 197 926 549 258 1190 901 262 1553
Fragments arranged by size:
1553 1190 926 901 549 262 258 197
SnaBI TAC'GTA
Cuts at: 493 3627 493
Size: 3134 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5836
SphI G_CATG'C
Cuts at: 1132 3194 1132
Size: 2062 3774
SspI AAT'ATT
Cuts at: 5 52 3760 5
Size: 47 3708 2081
Fragments arranged by size:
3708 2081 47
StuI AGG'CCT
Cuts at: 1517 2339 2420 1517
Size: 822 81 4933
Fragments arranged by size:
4933 822 81
StyI C'CwwG_G
Cuts at: 513 1065 3275 513
Size: 552 2210 3074
Fragments arranged by size:
3074 2210 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2213 2491 3863 3880 4033 4218 4663
Size: 1255 633 278 1372 17 153 185 445
Cuts at: 4663 325
Size: 1498
Fragments arranged by size:
1498 1372 1255 633 445 278 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2712 4082 5794
Size: 274 80 33 51 1443 735 1370 1712
Cuts at: 5794 96
Size: 138
Fragments arranged by size:
1712 1443 1370 735 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2070 2102 5796 5828 2070
Size: 32 3694 32 2078
Fragments arranged by size:
3694 2078 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2792 2867 1316
Size: 1476 75 4285
Fragments arranged by size:
4285 1476 75
XbaI T'CTAG_A
Cuts at: 3172 3172
Size: 5836
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5836
XmaI C'CCGG_G
Cuts at: 1870 2127 3161 1870
Size: 257 1034 4545
Fragments arranged by size:
4545 1034 257
XmnI GAAnn'nnTTC
Cuts at: 3965 3965
Size: 5836
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3644 276
Size: 53 83 186 936 2110 2468
Fragments arranged by size:
2468 2110 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI
FspAI KasI NarI NruI NsiI PacI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SfiI SfoI
SrfI SwaI XcmI