Restriction map for
pFN21AE0541
(Circular) (Six-base) MAPSORT of: pFN21AE0541.seq Check: 9595 from: 1 to: 5368
pFN21AE0541 5368 bp
With 182 enzymes: SgfI *
October 20, 2009 10:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3178 278
Size: 53 83 186 936 1642 2468
Fragments arranged by size:
2468 1642 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2711 1937
Size: 774 4594
Acc65I G'GTAC_C
Cuts at: 2689 2689
Size: 5368
AclI AA'CG_TT
Cuts at: 1563 3495 3868 1563
Size: 1932 373 3063
Fragments arranged by size:
3063 1932 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2150 3431 4815 5083 1632
Size: 518 1281 1384 268 1917
Fragments arranged by size:
1917 1384 1281 518 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5171
AflIII A'CryG_T
Cuts at: 1203 4267 5244 1203
Size: 3064 977 1327
Fragments arranged by size:
3064 1327 977
AgeI A'CCGG_T
Cuts at: 2096 4199 2096
Size: 2103 3265
AhdI GACnn_n'nnGTC
Cuts at: 4097 4097
Size: 5368
AleI CACnn'nnGTG
Cuts at: 1166 2327 2507 1166
Size: 1161 180 4027
Fragments arranged by size:
4027 1161 180
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2363 1184
Size: 1179 4189
AlwNI CAG_nnn'CTG
Cuts at: 3111 4683 3111
Size: 1572 3796
ApaLI G'TGCA_C
Cuts at: 3425 4581 3425
Size: 1156 4212
ApoI r'AATT_y
Cuts at: 1493 2677 2903 3055 5164 1493
Size: 1184 226 152 2109 1697
Fragments arranged by size:
2109 1697 1184 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3922 160
Size: 872 2890 1606
Fragments arranged by size:
2890 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5368
AvaI C'yCGr_G
Cuts at: 1870 1943 2693 1870
Size: 73 750 4545
Fragments arranged by size:
4545 750 73
BamHI G'GATC_C
Cuts at: 2186 2253 2698 3065 2186
Size: 67 445 367 4489
Fragments arranged by size:
4489 445 367 67
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2084 2277 2501 2689 4144
Size: 325 217 627 297 193 224 188 1455
Cuts at: 4144 618
Size: 1842
Fragments arranged by size:
1842 1455 627 325 297 224 217 193
188
BanII G_rGCy'C
Cuts at: 1391 2109 2646 2687 1391
Size: 718 537 41 4072
Fragments arranged by size:
4072 718 537 41
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2415 928
Size: 971 516 3881
Fragments arranged by size:
3881 971 516
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3559 3593 1903
Size: 34 1622 34 3678
Fragments arranged by size:
3678 1622 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3260 4470 3260
Size: 1210 4158
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5368
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3979 136
Size: 107 122 71 631 605 2307 1525
Fragments arranged by size:
2307 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5363 5363
Size: 5368
BlpI GC'TnA_GC
Cuts at: 2785 2785
Size: 5368
Bme1580I G_kGCm'C
Cuts at: 1790 2282 2504 3429 4585 1790
Size: 492 222 925 1156 2573
Fragments arranged by size:
2573 1156 925 492 222
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5368
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2473 2574 3099
Size: 416 53 83 399 24 1056 101 525
Cuts at: 3099 4057 442
Size: 958 1753
Fragments arranged by size:
1753 1056 958 525 416 399 101 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5368
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4028 1239
Size: 2789 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3493 4358 4656 3493
Size: 865 298 4205
Fragments arranged by size:
4205 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4031 882
Size: 617 2532 2219
Fragments arranged by size:
2532 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2601 3159 493
Size: 711 1397 558 2702
Fragments arranged by size:
2702 1397 711 558
BsaBI GATnn'nnATC
Cuts at: 5362 5362
Size: 5368
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2225 2235 3175 3557
Size: 53 83 186 936 692 10 940 382
Cuts at: 3557 4192 275
Size: 635 1451
Fragments arranged by size:
1451 940 936 692 635 382 186 83
53 10
BsaWI w'CCGG_w
Cuts at: 2096 3801 4199 4473 4620 5031 2096
Size: 1705 398 274 147 411 2433
Fragments arranged by size:
2433 1705 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2261 2291 1178
Size: 30 165 30 858 30 4255
Fragments arranged by size:
4255 858 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5368
BseYI C'CCAG_C
Cuts at: 4571 5067 4571
Size: 496 4872
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2147 2333 1163
Size: 573 411 186 4198
Fragments arranged by size:
4198 573 411 186
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2818 3579 4607 1540
Size: 275 186 817 761 1028 2301
Fragments arranged by size:
2301 1028 817 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2109 3429 3514 4585 2109
Size: 1320 85 1071 2892
Fragments arranged by size:
2892 1320 1071 85
BsmI GAATG_Cn'
Cuts at: 2879 2972 2879
Size: 93 5275
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2109 2282 2504 2646 2687 3429 3514
Size: 399 319 173 222 142 41 742 85
Cuts at: 3514 4585 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 319 222 173 142
85 41
BspEI T'CCGG_A
Cuts at: 5031 5031
Size: 5368
BspHI T'CATG_A
Cuts at: 3257 4987 3257
Size: 1730 3638
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2123 2355 2642 2723 844
Size: 1279 232 287 81 3489
Fragments arranged by size:
3489 1279 287 232 81
BsrBI CCG'CTC
Cuts at: 1394 1823 3255 1394
Size: 429 1432 3507
Fragments arranged by size:
3507 1432 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3863 4037 65
Size: 1183 2615 174 1396
Fragments arranged by size:
2615 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2038 2096 2213 4012 4199 1700
Size: 110 228 58 117 1799 187 2869
Fragments arranged by size:
2869 1799 228 187 117 110 58
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5368
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5368
BssSI C'ACGA_G
Cuts at: 2108 3428 4440 2108
Size: 1320 1012 3036
Fragments arranged by size:
3036 1320 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5368
BstBI TT'CG_AA
Cuts at: 4172 4172
Size: 5368
BstEII G'GTnAC_C
Cuts at: 1184 2209 1184
Size: 1025 4343
BstYI r'GATC_y
Cuts at: 1972 2186 2253 2698 3065 3450 3467 4919 5005
Size: 214 67 445 367 385 17 1452 86
Cuts at: 5005 5017 5363 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 445 385 367 346 214 86
67 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2020 2257 2551 2569 2610 513
Size: 552 955 237 294 18 41 3271
Fragments arranged by size:
3271 955 552 294 237 41 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4629
Fragments arranged by size:
4629 683 56
BtsI GCAGTG_nn'
Cuts at: 2884 3678 3698 2884
Size: 794 20 4554
Fragments arranged by size:
4554 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5368
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5333
DraI TTT'AAA
Cuts at: 2672 2672
Size: 5368
DraIII CAC_nnn'GTG
Cuts at: 2155 2155
Size: 5368
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2119 4375 809
Size: 507 803 2256 1802
Fragments arranged by size:
2256 1802 803 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2036 2197 2815
Size: 54 1425 50 275 18 206 161 618
Cuts at: 2815 3704 5333 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 618 275 206 161 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2815 1537
Size: 275 1003 4090
Fragments arranged by size:
4090 1003 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3298 1353
Size: 1945 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2266 3955 4329 4475 387
Size: 1064 138 677 1689 374 146 1280
Fragments arranged by size:
1689 1280 1064 677 374 146 138
EcoICRI GAG'CTC
Cuts at: 2107 2107
Size: 5368
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2150 3431 4028 4815 5083 1239
Size: 393 518 1281 597 787 268 1524
Fragments arranged by size:
1524 1281 787 597 518 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2289 2289
Size: 5368
EcoRI G'AATT_C
Cuts at: 2677 3055 2677
Size: 378 4990
FspI TGC'GCA
Cuts at: 3874 3874
Size: 5368
HaeII r_GCGC'y
Cuts at: 1678 2066 4515 1678
Size: 388 2449 2531
Fragments arranged by size:
2531 2449 388
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4009 4041 4083 4115 1373
Size: 32 135 32 2437 32 42 32 2626
Fragments arranged by size:
2626 2437 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2712 2958 669
Size: 988 281 774 246 3079
Fragments arranged by size:
3079 988 774 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5368
HpaI GTT'AAC
Cuts at: 2958 2958
Size: 5368
KpnI G_GTAC'C
Cuts at: 2693 2693
Size: 5368
MfeI C'AATT_G
Cuts at: 2967 2967
Size: 5368
MluI A'CGCG_T
Cuts at: 5244 5244
Size: 5368
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2208 2403 4482 4666 1305
Size: 903 195 2079 184 2007
Fragments arranged by size:
2079 2007 903 195 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3546
Fragments arranged by size:
3546 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2327 2507 3326 3685 3844 518
Size: 648 1161 180 819 359 159 2042
Fragments arranged by size:
2042 1161 819 648 359 180 159
MspA1I CmG'CkG
Cuts at: 1606 2022 2196 2259 2374 2784 3461 4609 4854
Size: 416 174 63 115 410 677 1148 245
Cuts at: 4854 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 416 410 245 174 115
63
NcoI C'CATG_G
Cuts at: 513 1065 2610 513
Size: 552 1545 3271
Fragments arranged by size:
3271 1545 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5368
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5368
NotI GC'GGCC_GC
Cuts at: 2815 2815
Size: 5368
NspI r_CATG'y
Cuts at: 1132 1229 2330 2498 2578 2726 4271 1132
Size: 97 1101 168 80 148 1545 2229
Fragments arranged by size:
2229 1545 1101 168 148 97 80
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5368
PciI A'CATG_T
Cuts at: 4267 4267
Size: 5368
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2497 1108
Size: 1389 3979
PmeI GTTT'AAAC
Cuts at: 2672 2672
Size: 5368
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3437 3469 4950 4982 3437
Size: 32 1481 32 3823
Fragments arranged by size:
3823 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2289 2289
Size: 5368
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5368
PsiI TTA'TAA
Cuts at: 2938 2938
Size: 5368
PstI C_TGCA'G
Cuts at: 830 2653 2720 830
Size: 1823 67 3478
Fragments arranged by size:
3478 1823 67
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5368
SacI G_AGCT'C
Cuts at: 2109 2109
Size: 5368
SacII CC_GC'GG
Cuts at: 2023 2260 2023
Size: 237 5131
SalI G'TCGA_C
Cuts at: 1936 2710 1936
Size: 774 4594
SanDI GG'GwC_CC
Cuts at: 2289 2289
Size: 5368
SbfI CC_TGCA'GG
Cuts at: 2720 2720
Size: 5368
ScaI AGT'ACT
Cuts at: 3616 5328 3616
Size: 1712 3656
SfcI C'TryA_G
Cuts at: 826 1045 2649 2716 3851 4532 4723 826
Size: 219 1604 67 1135 681 191 1471
Fragments arranged by size:
1604 1471 1135 681 219 191 67
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5368
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5368
SmaI CCC'GGG
Cuts at: 1872 2695 1872
Size: 823 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3472 4373 4635 820
Size: 197 926 1529 901 262 1553
Fragments arranged by size:
1553 1529 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3159 493
Size: 2666 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5368
SphI G_CATG'C
Cuts at: 1132 2578 2726 1132
Size: 1446 148 3774
Fragments arranged by size:
3774 1446 148
SspI AAT'ATT
Cuts at: 5 52 3292 5
Size: 47 3240 2081
Fragments arranged by size:
3240 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5368
StyI C'CwwG_G
Cuts at: 513 1065 2610 2807 513
Size: 552 1545 197 3074
Fragments arranged by size:
3074 1545 552 197
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3395 3412 3565 3750 4195 325
Size: 1255 1815 17 153 185 445 1498
Fragments arranged by size:
1815 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3614 5326 96
Size: 274 80 33 51 1443 1637 1712 138
Fragments arranged by size:
1712 1637 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2261 2293 5328 5360 2261
Size: 32 3035 32 2269
Fragments arranged by size:
3035 2269 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5368
XbaI T'CTAG_A
Cuts at: 2704 2704
Size: 5368
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5368
XmaI C'CCGG_G
Cuts at: 1870 2693 1870
Size: 823 4545
XmnI GAAnn'nnTTC
Cuts at: 3497 3497
Size: 5368
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3176 276
Size: 53 83 186 936 1642 2468
Fragments arranged by size:
2468 1642 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI
SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI PvuII RsrII SapI SexAI SfiI SfoI
SrfI SwaI XcmI