Restriction map for
pFN21AE0540
(Circular) (Six-base) MAPSORT of: pFN21AE0540.seq Check: 8522 from: 1 to: 5503
pFN21AE0540 5503 bp
With 182 enzymes: SgfI *
October 20, 2009 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2328 2431 2328
Size: 103 5400
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3313 278
Size: 53 83 186 936 1777 2468
Fragments arranged by size:
2468 1777 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2846 1937
Size: 909 4594
Acc65I G'GTAC_C
Cuts at: 2824 2824
Size: 5503
AclI AA'CG_TT
Cuts at: 1563 3630 4003 1563
Size: 2067 373 3063
Fragments arranged by size:
3063 2067 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3566 4950 5218 1632
Size: 1934 1384 268 1917
Fragments arranged by size:
1934 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5306
AflIII A'CryG_T
Cuts at: 1203 4402 5379 1203
Size: 3199 977 1327
Fragments arranged by size:
3199 1327 977
AgeI A'CCGG_T
Cuts at: 4334 4334
Size: 5503
AhdI GACnn_n'nnGTC
Cuts at: 4232 4232
Size: 5503
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5503
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2148 1184
Size: 964 4539
AlwNI CAG_nnn'CTG
Cuts at: 2095 2468 3246 4818 2095
Size: 373 778 1572 2780
Fragments arranged by size:
2780 1572 778 373
ApaI G_GGCC'C
Cuts at: 2132 2168 2132
Size: 36 5467
ApaLI G'TGCA_C
Cuts at: 3560 4716 3560
Size: 1156 4347
ApoI r'AATT_y
Cuts at: 1493 2812 3038 3190 5299 1493
Size: 1319 226 152 2109 1697
Fragments arranged by size:
2109 1697 1319 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4057 160
Size: 872 3025 1606
Fragments arranged by size:
3025 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5503
AvaI C'yCGr_G
Cuts at: 1870 1943 2510 2828 1870
Size: 73 567 318 4545
Fragments arranged by size:
4545 567 318 73
BamHI G'GATC_C
Cuts at: 2833 3200 2833
Size: 367 5136
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2824 4279 618
Size: 325 217 627 1037 1455 1842
Fragments arranged by size:
1842 1455 1037 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2027 2132 2168 2344 2822 1391
Size: 636 105 36 176 478 4072
Fragments arranged by size:
4072 636 478 176 105 36
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2485 928
Size: 971 586 3946
Fragments arranged by size:
3946 971 586
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3694 3728 1903
Size: 34 50 34 1673 34 3678
Fragments arranged by size:
3678 1673 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3395 4605 3395
Size: 1210 4293
BclI T'GATC_A
Cuts at: 1548 2190 1548
Size: 642 4861
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2231 2240 4114
Size: 107 122 71 631 605 559 9 1874
Cuts at: 4114 136
Size: 1525
Fragments arranged by size:
1874 1525 631 605 559 122 107 71
9
BglII A'GATC_T
Cuts at: 2751 5498 2751
Size: 2747 2756
BlpI GC'TnA_GC
Cuts at: 2745 2920 2745
Size: 175 5328
Bme1580I G_kGCm'C
Cuts at: 1790 2132 2168 3564 4720 1790
Size: 342 36 1396 1156 2573
Fragments arranged by size:
2573 1396 1156 342 36
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5503
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3234 4192 442
Size: 416 53 83 399 24 1817 958 1753
Fragments arranged by size:
1817 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5503
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2770 2770
Size: 5503
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2447 2463 4163 1239
Size: 1208 16 1700 2579
Fragments arranged by size:
2579 1700 1208 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2039 3628 4493 4791 2039
Size: 1589 865 298 2751
Fragments arranged by size:
2751 1589 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4166 882
Size: 617 2667 2219
Fragments arranged by size:
2667 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3294 493
Size: 711 2090 2702
Fragments arranged by size:
2702 2090 711
BsaBI GATnn'nnATC
Cuts at: 5497 5497
Size: 5503
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3310 3692 4327 275
Size: 53 83 186 936 1777 382 635 1451
Fragments arranged by size:
1777 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2062 3936 4334 4608 4755 5166 2062
Size: 1874 398 274 147 411 2399
Fragments arranged by size:
2399 1874 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5278
Fragments arranged by size:
5278 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5503
BseYI C'CCAG_C
Cuts at: 4706 5202 4706
Size: 496 5007
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2459 2504 1163
Size: 573 723 45 4162
Fragments arranged by size:
4162 723 573 45
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2731 2953 3714 4742 1540
Size: 275 186 730 222 761 1028 2301
Fragments arranged by size:
2301 1028 761 730 275 222 186
BsiHKAI G_wGCw'C
Cuts at: 3564 3649 4720 3564
Size: 85 1071 4347
Fragments arranged by size:
4347 1071 85
BsmI GAATG_Cn'
Cuts at: 3014 3107 3014
Size: 93 5410
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2027 2132 2168 2344 2822 3564 3649
Size: 399 237 105 36 176 478 742 85
Cuts at: 3649 4720 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 478 399 237 176 105
85 36
BspEI T'CCGG_A
Cuts at: 5166 5166
Size: 5503
BspHI T'CATG_A
Cuts at: 3392 5122 3392
Size: 1730 3773
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2328 2431 2700 2858 844
Size: 1484 103 269 158 3489
Fragments arranged by size:
3489 1484 269 158 103
BsrBI CCG'CTC
Cuts at: 1394 1823 2515 3390 1394
Size: 429 692 875 3507
Fragments arranged by size:
3507 875 692 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2679 3998 4172 65
Size: 1183 1431 1319 174 1396
Fragments arranged by size:
1431 1396 1319 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2579 2636 4147 4334 1700
Size: 110 769 57 1511 187 2869
Fragments arranged by size:
2869 1511 769 187 110 57
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5503
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5503
BssSI C'ACGA_G
Cuts at: 3563 4575 3563
Size: 1012 4491
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2446 794
Size: 1652 3851
BstBI TT'CG_AA
Cuts at: 2176 4307 2176
Size: 2131 3372
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5503
BstXI CCAn_nnnn'nTGG
Cuts at: 2168 2168
Size: 5503
BstYI r'GATC_y
Cuts at: 1972 2751 2833 3200 3585 3602 5054 5140 5152
Size: 779 82 367 385 17 1452 86 12
Cuts at: 5152 5498 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 779 385 367 346 86 82
17 12
Bsu36I CC'TnA_GG
Cuts at: 2237 2237
Size: 5503
BtgI C'CryG_G
Cuts at: 513 1065 2532 513
Size: 552 1467 3484
Fragments arranged by size:
3484 1467 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4764
Fragments arranged by size:
4764 683 56
BtsI GCAGTG_nn'
Cuts at: 2398 2797 3019 3813 3833 2398
Size: 399 222 794 20 4068
Fragments arranged by size:
4068 794 399 222 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5503
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5468
DraI TTT'AAA
Cuts at: 2807 2807
Size: 5503
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2571 4510 809
Size: 507 1255 1939 1802
Fragments arranged by size:
1939 1802 1255 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2728 2950 3839
Size: 54 1425 50 275 18 898 222 889
Cuts at: 3839 5468 8
Size: 1629 43
Fragments arranged by size:
1629 1425 898 889 275 222 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2728 2950 1537
Size: 275 916 222 4090
Fragments arranged by size:
4090 916 275 222
EarI CTCTTCn'nnn_
Cuts at: 1353 2717 3433 1353
Size: 1364 716 3423
Fragments arranged by size:
3423 1364 716
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4090 4464 4610 387
Size: 1064 138 2501 374 146 1280
Fragments arranged by size:
2501 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2447 2463 3566 4163 4950 5218 1239
Size: 393 815 16 1103 597 787 268 1524
Fragments arranged by size:
1524 1103 815 787 597 393 268 16
EcoNI CCTnn'n_nnAGG
Cuts at: 2288 2288
Size: 5503
EcoO109I rG'GnC_Cy
Cuts at: 2076 2092 2106 2128 2129 2164 2165 2223 2076
Size: 16 14 22 1 35 1 58 5356
Fragments arranged by size:
5356 58 35 22 16 14 1 1
EcoRI G'AATT_C
Cuts at: 2812 3190 2812
Size: 378 5125
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2374 2374
Size: 5503
FspI TGC'GCA
Cuts at: 4009 4009
Size: 5503
HaeII r_GCGC'y
Cuts at: 1678 4650 1678
Size: 2972 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4144 4176 4218 4250 1373
Size: 32 135 32 2572 32 42 32 2626
Fragments arranged by size:
2626 2572 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2847 3093 669
Size: 988 281 909 246 3079
Fragments arranged by size:
3079 988 909 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5503
HpaI GTT'AAC
Cuts at: 3093 3093
Size: 5503
KpnI G_GTAC'C
Cuts at: 2828 2828
Size: 5503
MfeI C'AATT_G
Cuts at: 3102 3102
Size: 5503
MluI A'CGCG_T
Cuts at: 5379 5379
Size: 5503
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4617 4801 1305
Size: 3312 184 2007
Fragments arranged by size:
3312 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3681
Fragments arranged by size:
3681 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2501 3461 3820 3979 518
Size: 648 1335 960 359 159 2042
Fragments arranged by size:
2042 1335 960 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2297 2455 2919 3596 4744 4989 1606
Size: 691 158 464 677 1148 245 2120
Fragments arranged by size:
2120 1148 691 677 464 245 158
NaeI GCC'GGC
Cuts at: 2638 2638
Size: 5503
NcoI C'CATG_G
Cuts at: 513 1065 2532 513
Size: 552 1467 3484
Fragments arranged by size:
3484 1467 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5503
NgoMIV G'CCGG_C
Cuts at: 2636 2636
Size: 5503
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5503
NotI GC'GGCC_GC
Cuts at: 2950 2950
Size: 5503
NspI r_CATG'y
Cuts at: 1132 1229 2861 4406 1132
Size: 97 1632 1545 2229
Fragments arranged by size:
2229 1632 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2072 2080 2161 2168 1348
Size: 724 8 81 7 4683
Fragments arranged by size:
4683 724 81 8 7
PciI A'CATG_T
Cuts at: 4402 4402
Size: 5503
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2034 2323 1108
Size: 926 289 4288
Fragments arranged by size:
4288 926 289
PfoI T'CCnGG_A
Cuts at: 2547 2547
Size: 5503
PmeI GTTT'AAAC
Cuts at: 2807 2807
Size: 5503
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3572 3604 5085 5117 3572
Size: 32 1481 32 3958
Fragments arranged by size:
3958 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2076 2106 2076
Size: 30 5473
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5503
PsiI TTA'TAA
Cuts at: 3073 3073
Size: 5503
PspOMI G'GGCC_C
Cuts at: 2128 2164 2128
Size: 36 5467
PstI C_TGCA'G
Cuts at: 830 2137 2262 2855 830
Size: 1307 125 593 3478
Fragments arranged by size:
3478 1307 593 125
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5503
PvuII CAG'CTG
Cuts at: 2297 2455 2297
Size: 158 5345
SalI G'TCGA_C
Cuts at: 1936 2845 1936
Size: 909 4594
SanDI GG'GwC_CC
Cuts at: 2076 2076
Size: 5503
SbfI CC_TGCA'GG
Cuts at: 2855 2855
Size: 5503
ScaI AGT'ACT
Cuts at: 3751 5463 3751
Size: 1712 3791
SfcI C'TryA_G
Cuts at: 826 1045 2133 2258 2851 3986 4667 4858 826
Size: 219 1088 125 593 1135 681 191 1471
Fragments arranged by size:
1471 1135 1088 681 593 219 191 125
SfiI GGCCn_nnn'nGGCC
Cuts at: 2231 2231
Size: 5503
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5503
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5503
SmaI CCC'GGG
Cuts at: 1872 2830 1872
Size: 958 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2054 3607 4508 4770 820
Size: 197 926 111 1553 901 262 1553
Fragments arranged by size:
1553 1553 926 901 262 197 111
SnaBI TAC'GTA
Cuts at: 493 3294 493
Size: 2801 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5503
SphI G_CATG'C
Cuts at: 1132 2861 1132
Size: 1729 3774
SspI AAT'ATT
Cuts at: 5 52 3427 5
Size: 47 3375 2081
Fragments arranged by size:
3375 2081 47
StuI AGG'CCT
Cuts at: 1517 2199 1517
Size: 682 4821
StyI C'CwwG_G
Cuts at: 513 1065 2532 2643 2942 513
Size: 552 1467 111 299 3074
Fragments arranged by size:
3074 1467 552 299 111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2362 2574 3530 3547 3700 3885 4330
Size: 1255 782 212 956 17 153 185 445
Cuts at: 4330 325
Size: 1498
Fragments arranged by size:
1498 1255 956 782 445 212 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2382 2625 3749
Size: 274 80 33 51 1443 405 243 1124
Cuts at: 3749 5461 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1124 405 274 243 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5463 5495 5463
Size: 32 5471
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5503
XbaI T'CTAG_A
Cuts at: 2839 2839
Size: 5503
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5503
XmaI C'CCGG_G
Cuts at: 1870 2828 1870
Size: 958 4545
XmnI GAAnn'nnTTC
Cuts at: 3632 3632
Size: 5503
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3311 276
Size: 53 83 186 936 1777 2468
Fragments arranged by size:
2468 1777 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SanDI SbfI ScaI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI
FspAI KasI NarI NruI NsiI PacI PmlI PspXI
PsrI RsrII SacI SacII SapI SexAI SfoI SrfI
SwaI XcmI