Restriction map for
pFN21AE0534
(Circular) (Six-base) MAPSORT of: pFN21AE0534.seq Check: 8141 from: 1 to: 6028
pFN21AE0534 6028 bp
With 182 enzymes: SgfI *
October 20, 2009 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3238 3238
Size: 6028
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3838 278
Size: 53 83 186 936 2302 2468
Fragments arranged by size:
2468 2302 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2424 3371 1937
Size: 487 947 4594
Fragments arranged by size:
4594 947 487
Acc65I G'GTAC_C
Cuts at: 3349 3349
Size: 6028
AclI AA'CG_TT
Cuts at: 1563 4155 4528 1563
Size: 2592 373 3063
Fragments arranged by size:
3063 2592 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2605 2875 4091 5475 5743 1632
Size: 973 270 1216 1384 268 1917
Fragments arranged by size:
1917 1384 1216 973 270 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5831
AflIII A'CryG_T
Cuts at: 1203 2328 4927 5904 1203
Size: 1125 2599 977 1327
Fragments arranged by size:
2599 1327 1125 977
AgeI A'CCGG_T
Cuts at: 4859 4859
Size: 6028
AhdI GACnn_n'nnGTC
Cuts at: 4757 4757
Size: 6028
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6028
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6028
AlwNI CAG_nnn'CTG
Cuts at: 2027 2109 2134 2514 3230 3771 5343 2027
Size: 82 25 380 716 541 1572 2712
Fragments arranged by size:
2712 1572 716 541 380 82 25
ApaLI G'TGCA_C
Cuts at: 4085 5241 4085
Size: 1156 4872
ApoI r'AATT_y
Cuts at: 1493 3337 3563 3715 5824 1493
Size: 1844 226 152 2109 1697
Fragments arranged by size:
2109 1844 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4582 160
Size: 872 3550 1606
Fragments arranged by size:
3550 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6028
AvaI C'yCGr_G
Cuts at: 1870 1943 2560 3353 1870
Size: 73 617 793 4545
Fragments arranged by size:
4545 793 617 73
BamHI G'GATC_C
Cuts at: 3358 3725 3358
Size: 367 5661
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2120 2532 3029 3349 4804
Size: 325 217 627 333 412 497 320 1455
Cuts at: 4804 618
Size: 1842
Fragments arranged by size:
1842 1455 627 497 412 333 325 320
217
BanII G_rGCy'C
Cuts at: 1391 2042 3006 3224 3347 1391
Size: 651 964 218 123 4072
Fragments arranged by size:
4072 964 651 218 123
BbeI G_GCGC'C
Cuts at: 2124 2124
Size: 6028
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5057
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2551 2585 4219 4253 1903
Size: 34 614 34 1634 34 3678
Fragments arranged by size:
3678 1634 614 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3920 5130 3920
Size: 1210 4818
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6028
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2563 3200 4639
Size: 107 122 71 631 605 891 637 1439
Cuts at: 4639 136
Size: 1525
Fragments arranged by size:
1525 1439 891 637 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2520 6023 2520
Size: 3503 2525
BlpI GC'TnA_GC
Cuts at: 3223 3445 3223
Size: 222 5806
Bme1580I G_kGCm'C
Cuts at: 1790 2012 2537 2559 3032 4089 5245 1790
Size: 222 525 22 473 1057 1156 2573
Fragments arranged by size:
2573 1156 1057 525 473 222 22
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6028
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2212 2821 3035
Size: 416 53 83 399 24 795 609 214
Cuts at: 3035 3759 4717 442
Size: 724 958 1753
Fragments arranged by size:
1753 958 795 724 609 416 399 214
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2021 1056
Size: 965 5063
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2545 2775 3133 4688 1239
Size: 1306 230 358 1555 2579
Fragments arranged by size:
2579 1555 1306 358 230
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2930 4153 5018 5316 2930
Size: 1223 865 298 3642
Fragments arranged by size:
3642 1223 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2651 4691 882
Size: 617 1152 2040 2219
Fragments arranged by size:
2219 2040 1152 617
BsaAI yAC'GTr
Cuts at: 493 1204 3819 493
Size: 711 2615 2702
Fragments arranged by size:
2702 2615 711
BsaBI GATnn'nnATC
Cuts at: 6022 6022
Size: 6028
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2121 2360 3835 4217
Size: 53 83 186 936 588 239 1475 382
Cuts at: 4217 4852 275
Size: 635 1451
Fragments arranged by size:
1475 1451 936 635 588 382 239 186
83 53
BsaWI w'CCGG_w
Cuts at: 4461 4859 5133 5280 5691 4461
Size: 398 274 147 411 4798
Fragments arranged by size:
4798 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2313 2343 1178
Size: 30 165 30 910 30 4863
Fragments arranged by size:
4863 910 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2168 3143 1186
Size: 982 975 4071
Fragments arranged by size:
4071 982 975
BseYI C'CCAG_C
Cuts at: 2061 2418 2782 3046 3251 5231 5727 2061
Size: 357 364 264 205 1980 496 2362
Fragments arranged by size:
2362 1980 496 364 357 264 205
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3216 1163
Size: 573 1480 3975
Fragments arranged by size:
3975 1480 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3018 3478 4239 5267 1540
Size: 275 186 1017 460 761 1028 2301
Fragments arranged by size:
2301 1028 1017 761 460 275 186
BsiHKAI G_wGCw'C
Cuts at: 2394 3224 4089 4174 5245 2394
Size: 830 865 85 1071 3177
Fragments arranged by size:
3177 1071 865 830 85
BsmI GAATG_Cn'
Cuts at: 3539 3632 3539
Size: 93 5935
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2042 2394 2537 2559 3006 3032
Size: 399 222 30 352 143 22 447 26
Cuts at: 3032 3224 3347 4089 4174 5245 1391
Size: 192 123 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 447 399 352 222 192
143 123 85 30 26 22
BspEI T'CCGG_A
Cuts at: 5691 5691
Size: 6028
BspHI T'CATG_A
Cuts at: 3917 5647 3917
Size: 1730 4298
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3081 3238 3383 844
Size: 2237 157 145 3489
Fragments arranged by size:
3489 2237 157 145
BsrBI CCG'CTC
Cuts at: 1394 1823 2203 3915 1394
Size: 429 380 1712 3507
Fragments arranged by size:
3507 1712 429 380
BsrDI GCAATG_nn'
Cuts at: 65 1248 4523 4697 65
Size: 1183 3275 174 1396
Fragments arranged by size:
3275 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2280 4672 4859 1700
Size: 110 470 2392 187 2869
Fragments arranged by size:
2869 2392 470 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6028
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6028
BssSI C'ACGA_G
Cuts at: 2741 4088 5100 2741
Size: 1347 1012 3669
Fragments arranged by size:
3669 1347 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2134 794
Size: 1340 4688
BstBI TT'CG_AA
Cuts at: 4832 4832
Size: 6028
BstEII G'GTnAC_C
Cuts at: 1184 2341 3055 1184
Size: 1157 714 4157
Fragments arranged by size:
4157 1157 714
BstXI CCAn_nnnn'nTGG
Cuts at: 2653 2653
Size: 6028
BstYI r'GATC_y
Cuts at: 1972 2520 3358 3725 4110 4127 5579 5665 5677
Size: 548 838 367 385 17 1452 86 12
Cuts at: 5677 6023 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 838 548 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2316 2316
Size: 6028
BtgI C'CryG_G
Cuts at: 513 1065 2464 2538 3012 3237 513
Size: 552 1399 74 474 225 3304
Fragments arranged by size:
3304 1399 552 474 225 74
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5289
Fragments arranged by size:
5289 683 56
BtsI GCAGTG_nn'
Cuts at: 2683 3243 3544 4338 4358 2683
Size: 560 301 794 20 4353
Fragments arranged by size:
4353 794 560 301 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6028
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5993
DraI TTT'AAA
Cuts at: 3332 3332
Size: 6028
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2975 5035 809
Size: 507 1659 2060 1802
Fragments arranged by size:
2060 1802 1659 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2295 2745 3015
Size: 54 1425 50 275 18 465 450 270
Cuts at: 3015 3201 3240 3475 4364 5993 8
Size: 186 39 235 889 1629 43
Fragments arranged by size:
1629 1425 889 465 450 275 270 235
186 54 50 43 39 18
EagI C'GGCC_G
Cuts at: 1537 1812 3015 3475 1537
Size: 275 1203 460 4090
Fragments arranged by size:
4090 1203 460 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2873 3958 1353
Size: 1520 1085 3423
Fragments arranged by size:
3423 1520 1085
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4615 4989 5135 387
Size: 1064 138 3026 374 146 1280
Fragments arranged by size:
3026 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3222 3222
Size: 6028
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2545 2605 2775 2875 3133 4091 4688
Size: 393 913 60 170 100 258 958 597
Cuts at: 4688 5475 5743 1239
Size: 787 268 1524
Fragments arranged by size:
1524 958 913 787 597 393 268 258
170 100 60
EcoO109I rG'GnC_Cy
Cuts at: 2206 2502 2511 2565 2665 2737 2760 2770 3139
Size: 296 9 54 100 72 23 10 369
Cuts at: 3139 2206
Size: 5095
Fragments arranged by size:
5095 369 296 100 72 54 23 10
9
EcoRI G'AATT_C
Cuts at: 3337 3715 3337
Size: 378 5650
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2650 2650
Size: 6028
FspI TGC'GCA
Cuts at: 4534 4534
Size: 6028
HaeII r_GCGC'y
Cuts at: 1678 2124 2177 5175 1678
Size: 446 53 2998 2531
Fragments arranged by size:
2998 2531 446 53
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2381 2413 2887 2919 4669
Size: 32 135 32 809 32 474 32 1750
Cuts at: 4669 4701 4743 4775 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1750 809 474 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2425 3372 3618 669
Size: 988 281 487 947 246 3079
Fragments arranged by size:
3079 988 947 487 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6028
HpaI GTT'AAC
Cuts at: 3618 3618
Size: 6028
KasI G'GCGC_C
Cuts at: 2120 2120
Size: 6028
KpnI G_GTAC'C
Cuts at: 3353 3353
Size: 6028
MfeI C'AATT_G
Cuts at: 3627 3627
Size: 6028
MluI A'CGCG_T
Cuts at: 5904 5904
Size: 6028
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5142 5326 1305
Size: 3837 184 2007
Fragments arranged by size:
3837 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2747 10
Size: 54 1425 343 915 3291
Fragments arranged by size:
3291 1425 915 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3274 3986 4345 4504 518
Size: 648 2108 712 359 159 2042
Fragments arranged by size:
2108 2042 712 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2869 3444 4121 5269 5514 1606
Size: 1263 575 677 1148 245 2120
Fragments arranged by size:
2120 1263 1148 677 575 245
NarI GG'CG_CC
Cuts at: 2121 2121
Size: 6028
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5476
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6028
NheI G'CTAG_C
Cuts at: 1052 2017 1052
Size: 965 5063
NotI GC'GGCC_GC
Cuts at: 3475 3475
Size: 6028
NspI r_CATG'y
Cuts at: 1132 1229 3386 4931 1132
Size: 97 2157 1545 2229
Fragments arranged by size:
2229 2157 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2045 1348
Size: 697 5331
PciI A'CATG_T
Cuts at: 4927 4927
Size: 6028
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2109 2488 2907 1108
Size: 1001 379 419 4229
Fragments arranged by size:
4229 1001 419 379
PfoI T'CCnGG_A
Cuts at: 3317 3317
Size: 6028
PmeI GTTT'AAAC
Cuts at: 3332 3332
Size: 6028
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2315 2347 4097 4129 5610 5642 2315
Size: 32 1750 32 1481 32 2701
Fragments arranged by size:
2701 1750 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2206 2502 2511 2665 2737 2760 2770 2206
Size: 296 9 154 72 23 10 5464
Fragments arranged by size:
5464 296 154 72 23 10 9
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6028
PsiI TTA'TAA
Cuts at: 2079 3598 2079
Size: 1519 4509
PspXI vC'TCGA_Gb
Cuts at: 2560 2560
Size: 6028
PstI C_TGCA'G
Cuts at: 830 2130 2142 2874 3380 830
Size: 1300 12 732 506 3478
Fragments arranged by size:
3478 1300 732 506 12
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6028
PvuII CAG'CTG
Cuts at: 2869 2869
Size: 6028
SacI G_AGCT'C
Cuts at: 3224 3224
Size: 6028
SalI G'TCGA_C
Cuts at: 1936 2423 3370 1936
Size: 487 947 4594
Fragments arranged by size:
4594 947 487
SanDI GG'GwC_CC
Cuts at: 2502 2665 2502
Size: 163 5865
SapI GCTCTTCn'nnn_
Cuts at: 2873 2873
Size: 6028
SbfI CC_TGCA'GG
Cuts at: 3380 3380
Size: 6028
ScaI AGT'ACT
Cuts at: 4276 5988 4276
Size: 1712 4316
SfcI C'TryA_G
Cuts at: 826 1045 2126 2138 2793 2870 3376 4511 5192
Size: 219 1081 12 655 77 506 1135 681
Cuts at: 5192 5383 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1081 681 655 506 219 191
77 12
SfoI GGC'GCC
Cuts at: 2122 2122
Size: 6028
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6028
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6028
SmaI CCC'GGG
Cuts at: 1872 3355 1872
Size: 1483 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2560 2945 4132 5033 5295 820
Size: 197 926 617 385 1187 901 262 1553
Fragments arranged by size:
1553 1187 926 901 617 385 262 197
SnaBI TAC'GTA
Cuts at: 493 3819 493
Size: 3326 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6028
SphI G_CATG'C
Cuts at: 1132 3386 1132
Size: 2254 3774
SspI AAT'ATT
Cuts at: 5 52 3952 5
Size: 47 3900 2081
Fragments arranged by size:
3900 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6028
StyI C'CwwG_G
Cuts at: 513 1065 2652 2748 3135 3467 513
Size: 552 1587 96 387 332 3074
Fragments arranged by size:
3074 1587 552 387 332 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2774 3250 3314 4055 4072 4225 4410
Size: 1255 1194 476 64 741 17 153 185
Cuts at: 4410 4855 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1194 741 476 445 185 153
64 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4274 5986 96
Size: 274 80 33 51 1443 2297 1712 138
Fragments arranged by size:
2297 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2313 2345 5988 6020 2313
Size: 32 3643 32 2321
Fragments arranged by size:
3643 2321 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6028
XbaI T'CTAG_A
Cuts at: 3364 3364
Size: 6028
XhoI C'TCGA_G
Cuts at: 1943 2560 1943
Size: 617 5411
XmaI C'CCGG_G
Cuts at: 1870 3353 1870
Size: 1483 4545
XmnI GAAnn'nnTTC
Cuts at: 2330 4157 2330
Size: 1827 4201
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3836 276
Size: 53 83 186 936 2302 2468
Fragments arranged by size:
2468 2302 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV
FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI
PspOMI PsrI RsrII SacII SexAI SfiI SrfI SwaI
XcmI