Restriction map for
pFN21AE0531
(Circular) (Six-base) MAPSORT of: pFN21AE0531.seq Check: 7165 from: 1 to: 5938
pFN21AE0531 5938 bp
With 182 enzymes: SgfI *
October 20, 2009 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2390 2390
Size: 5938
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3117 3748 278
Size: 53 83 186 936 1581 631 2468
Fragments arranged by size:
2468 1581 936 631 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2454 3117 3281 1937
Size: 517 663 164 4594
Fragments arranged by size:
4594 663 517 164
Acc65I G'GTAC_C
Cuts at: 2508 3198 3259 2508
Size: 690 61 5187
Fragments arranged by size:
5187 690 61
AclI AA'CG_TT
Cuts at: 1563 4065 4438 1563
Size: 2502 373 3063
Fragments arranged by size:
3063 2502 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2033 2366 3010 3165 4001 5385 5653 1632
Size: 401 333 644 155 836 1384 268 1917
Fragments arranged by size:
1917 1384 836 644 401 333 268 155
AfeI AGC'GCT
Cuts at: 2294 2294
Size: 5938
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5741
AflIII A'CryG_T
Cuts at: 1203 2035 4837 5814 1203
Size: 832 2802 977 1327
Fragments arranged by size:
2802 1327 977 832
AgeI A'CCGG_T
Cuts at: 4769 4769
Size: 5938
AhdI GACnn_n'nnGTC
Cuts at: 4667 4667
Size: 5938
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5938
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5938
AlwNI CAG_nnn'CTG
Cuts at: 2050 3681 5253 2050
Size: 1631 1572 2735
Fragments arranged by size:
2735 1631 1572
ApaI G_GGCC'C
Cuts at: 2503 2809 2503
Size: 306 5632
ApaLI G'TGCA_C
Cuts at: 3995 5151 3995
Size: 1156 4782
ApoI r'AATT_y
Cuts at: 1493 3247 3473 3625 5734 1493
Size: 1754 226 152 2109 1697
Fragments arranged by size:
2109 1754 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4492 160
Size: 872 3460 1606
Fragments arranged by size:
3460 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5938
AvaI C'yCGr_G
Cuts at: 1870 1943 2489 2802 3263 1870
Size: 73 546 313 461 4545
Fragments arranged by size:
4545 546 461 313 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2848 2881 2848
Size: 33 5905
BamHI G'GATC_C
Cuts at: 2284 2926 3268 3635 2284
Size: 642 342 367 4587
Fragments arranged by size:
4587 642 367 342
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2399 2508 3198 3259 4714
Size: 325 217 627 612 109 690 61 1455
Cuts at: 4714 618
Size: 1842
Fragments arranged by size:
1842 1455 690 627 612 325 217 109
61
BanII G_rGCy'C
Cuts at: 1391 2070 2503 2809 3257 1391
Size: 679 433 306 448 4072
Fragments arranged by size:
4072 679 448 433 306
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2021 3013 928
Size: 971 122 992 3853
Fragments arranged by size:
3853 992 971 122
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4129 4163 1903
Size: 34 50 34 2108 34 3678
Fragments arranged by size:
3678 2108 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3216 3830 5040 3216
Size: 614 1210 4114
Fragments arranged by size:
4114 1210 614
BclI T'GATC_A
Cuts at: 1548 2910 3168 1548
Size: 1362 258 4318
Fragments arranged by size:
4318 1362 258
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2813 4549 136
Size: 107 122 71 631 605 1141 1736 1525
Fragments arranged by size:
1736 1525 1141 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5933 5933
Size: 5938
BlpI GC'TnA_GC
Cuts at: 3355 3355
Size: 5938
Bme1580I G_kGCm'C
Cuts at: 1790 2503 2745 2809 3999 5155 1790
Size: 713 242 64 1190 1156 2573
Fragments arranged by size:
2573 1190 1156 713 242 64
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5938
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3669 4627 442
Size: 416 53 83 399 24 2252 958 1753
Fragments arranged by size:
2252 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3012 1056
Size: 1956 3982
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3242 4598 1239
Size: 2003 1356 2579
Fragments arranged by size:
2579 2003 1356
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2447 4063 4928 5226 2447
Size: 1616 865 298 3159
Fragments arranged by size:
3159 1616 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2789 4601 882
Size: 617 1290 1812 2219
Fragments arranged by size:
2219 1812 1290 617
BsaAI yAC'GTr
Cuts at: 493 1204 3729 493
Size: 711 2525 2702
Fragments arranged by size:
2702 2525 711
BsaBI GATnn'nnATC
Cuts at: 2915 5932 2915
Size: 3017 2921
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3114 3745 4127 4762
Size: 53 83 186 936 1581 631 382 635
Cuts at: 4762 275
Size: 1451
Fragments arranged by size:
1581 1451 936 635 631 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2184 4371 4769 5043 5190 5601 2184
Size: 2187 398 274 147 411 2521
Fragments arranged by size:
2521 2187 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2318 2348 3047 3077 1178
Size: 30 165 30 915 30 699 30 4039
Fragments arranged by size:
4039 915 699 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2507 3052 1186
Size: 1321 545 4072
Fragments arranged by size:
4072 1321 545
BseYI C'CCAG_C
Cuts at: 2029 3231 5141 5637 2029
Size: 1202 1910 496 2330
Fragments arranged by size:
2330 1910 1202 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5365
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3388 4149 5177 1540
Size: 275 186 1387 761 1028 2301
Fragments arranged by size:
2301 1387 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2025 2682 2709 3999 4084 5155 2025
Size: 657 27 1290 85 1071 2808
Fragments arranged by size:
2808 1290 1071 657 85 27
BsmI GAATG_Cn'
Cuts at: 2307 2539 3449 3542 2307
Size: 232 910 93 4703
Fragments arranged by size:
4703 910 232 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2025 2070 2503 2682 2709 2745 2809
Size: 399 235 45 433 179 27 36 64
Cuts at: 2809 3257 3999 4084 5155 1391
Size: 448 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 448 433 399 235 179
85 64 45 36 27
BspEI T'CCGG_A
Cuts at: 5601 5601
Size: 5938
BspHI T'CATG_A
Cuts at: 2907 3827 5557 2907
Size: 920 1730 3288
Fragments arranged by size:
3288 1730 920
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2390 2868 3226 3293 844
Size: 1546 478 358 67 3489
Fragments arranged by size:
3489 1546 478 358 67
BsrBI CCG'CTC
Cuts at: 1394 1823 2497 3825 1394
Size: 429 674 1328 3507
Fragments arranged by size:
3507 1328 674 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4433 4607 65
Size: 1183 3185 174 1396
Fragments arranged by size:
3185 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2732 4582 4769 1700
Size: 110 922 1850 187 2869
Fragments arranged by size:
2869 1850 922 187 110
BsrGI T'GTAC_A
Cuts at: 96 2982 96
Size: 2886 3052
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5938
BssSI C'ACGA_G
Cuts at: 3998 5010 3998
Size: 1012 4926
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5938
BstBI TT'CG_AA
Cuts at: 4742 4742
Size: 5938
BstEII G'GTnAC_C
Cuts at: 1184 2390 1184
Size: 1206 4732
BstYI r'GATC_y
Cuts at: 1972 2284 2926 3268 3635 4020 4037 5489 5575
Size: 312 642 342 367 385 17 1452 86
Cuts at: 5575 5587 5933 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 642 385 367 346 342 312
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2932 2932
Size: 5938
BtgI C'CryG_G
Cuts at: 513 1065 2085 513
Size: 552 1020 4366
Fragments arranged by size:
4366 1020 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2274 2536 487
Size: 683 56 1048 262 3889
Fragments arranged by size:
3889 1048 683 262 56
BtsI GCAGTG_nn'
Cuts at: 3454 4248 4268 3454
Size: 794 20 5124
Fragments arranged by size:
5124 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5938
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5903
DraI TTT'AAA
Cuts at: 3242 3242
Size: 5938
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2417 4945 809
Size: 507 1101 2528 1802
Fragments arranged by size:
2528 1802 1101 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2269 2570 2730
Size: 54 1425 50 275 18 439 301 160
Cuts at: 2730 3070 3385 4274 5903 8
Size: 340 315 889 1629 43
Fragments arranged by size:
1629 1425 889 439 340 315 301 275
160 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3385 1537
Size: 275 1573 4090
Fragments arranged by size:
4090 1573 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2171 3868 1353
Size: 818 1697 3423
Fragments arranged by size:
3423 1697 818
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2636 2863 4525 4899 5045 387
Size: 1064 138 1047 227 1662 374 146 1280
Fragments arranged by size:
1662 1280 1064 1047 374 227 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2033 2366 3010 3165 3242 4001 4598
Size: 393 401 333 644 155 77 759 597
Cuts at: 4598 5385 5653 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 759 644 597 401 393 333
268 155 77
EcoO109I rG'GnC_Cy
Cuts at: 2137 2137
Size: 5938
EcoRI G'AATT_C
Cuts at: 3247 3625 3247
Size: 378 5560
FseI GG_CCGG'CC
Cuts at: 2736 2736
Size: 5938
FspI TGC'GCA
Cuts at: 4444 4444
Size: 5938
HaeII r_GCGC'y
Cuts at: 1678 2296 3015 5085 1678
Size: 618 719 2070 2531
Fragments arranged by size:
2531 2070 719 618
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2239 2269 2271 2301 3104
Size: 32 135 32 667 30 2 30 803
Cuts at: 3104 3136 4579 4611 4653 4685 1373
Size: 32 1443 32 42 32 2626
Fragments arranged by size:
2626 1443 803 667 135 42 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 669 1657 1938 3282 3528 669
Size: 988 281 1344 246 3079
Fragments arranged by size:
3079 1344 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5938
HpaI GTT'AAC
Cuts at: 3528 3528
Size: 5938
KpnI G_GTAC'C
Cuts at: 2512 3202 3263 2512
Size: 690 61 5187
Fragments arranged by size:
5187 690 61
MfeI C'AATT_G
Cuts at: 3537 3537
Size: 5938
MluI A'CGCG_T
Cuts at: 5814 5814
Size: 5938
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3024 5052 5236 1305
Size: 1719 2028 184 2007
Fragments arranged by size:
2028 2007 1719 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2572 3072 10
Size: 54 1425 343 740 500 2876
Fragments arranged by size:
2876 1425 740 500 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2174 2384 2748 2978 3896 4255 4414
Size: 648 1008 210 364 230 918 359 159
Cuts at: 4414 518
Size: 2042
Fragments arranged by size:
2042 1008 918 648 364 359 230 210
159
MspA1I CmG'CkG
Cuts at: 1606 2115 2568 3030 3076 3354 4031 5179 5424
Size: 509 453 462 46 278 677 1148 245
Cuts at: 5424 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 509 462 453 278 245
46
NaeI GCC'GGC
Cuts at: 2734 2734
Size: 5938
NcoI C'CATG_G
Cuts at: 513 1065 2085 513
Size: 552 1020 4366
Fragments arranged by size:
4366 1020 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5938
NgoMIV G'CCGG_C
Cuts at: 2732 2732
Size: 5938
NheI G'CTAG_C
Cuts at: 1052 3008 1052
Size: 1956 3982
NotI GC'GGCC_GC
Cuts at: 3385 3385
Size: 5938
NruI TCG'CGA
Cuts at: 2259 2259
Size: 5938
NspI r_CATG'y
Cuts at: 1132 1229 2039 2461 2764 2989 3296 4841 1132
Size: 97 810 422 303 225 307 1545 2229
Fragments arranged by size:
2229 1545 810 422 307 303 225 97
PasI CC'CwG_GG
Cuts at: 1348 2338 2503 2738 1348
Size: 990 165 235 4548
Fragments arranged by size:
4548 990 235 165
PciI A'CATG_T
Cuts at: 2035 4837 2035
Size: 2802 3136
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2091 2884 1108
Size: 983 793 4162
Fragments arranged by size:
4162 983 793
PfoI T'CCnGG_A
Cuts at: 2421 2658 2421
Size: 237 5701
PmeI GTTT'AAAC
Cuts at: 3242 3242
Size: 5938
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4007 4039 5520 5552 4007
Size: 32 1481 32 4393
Fragments arranged by size:
4393 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2137 2137
Size: 5938
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5938
PsiI TTA'TAA
Cuts at: 3508 3508
Size: 5938
PspOMI G'GGCC_C
Cuts at: 2499 2805 2499
Size: 306 5632
PstI C_TGCA'G
Cuts at: 830 2076 2567 2724 2898 3035 3290 830
Size: 1246 491 157 174 137 255 3478
Fragments arranged by size:
3478 1246 491 255 174 157 137
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5938
PvuII CAG'CTG
Cuts at: 2568 3030 3076 2568
Size: 462 46 5430
Fragments arranged by size:
5430 462 46
SalI G'TCGA_C
Cuts at: 1936 3280 1936
Size: 1344 4594
SapI GCTCTTCn'nnn_
Cuts at: 2171 2171
Size: 5938
SbfI CC_TGCA'GG
Cuts at: 3290 3290
Size: 5938
ScaI AGT'ACT
Cuts at: 4186 5898 4186
Size: 1712 4226
SfcI C'TryA_G
Cuts at: 826 1045 2072 2563 2608 2720 2894 3031 3286
Size: 219 1027 491 45 112 174 137 255
Cuts at: 3286 4421 5102 5293 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1027 681 491 255 219 191
174 137 112 45
SfiI GGCCn_nnn'nGGCC
Cuts at: 2813 2813
Size: 5938
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5938
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5938
SmaI CCC'GGG
Cuts at: 1872 2804 3265 1872
Size: 932 461 4545
Fragments arranged by size:
4545 932 461
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2462 4042 4943 5205 820
Size: 197 926 519 1580 901 262 1553
Fragments arranged by size:
1580 1553 926 901 519 262 197
SnaBI TAC'GTA
Cuts at: 493 3729 493
Size: 3236 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5938
SphI G_CATG'C
Cuts at: 1132 3296 1132
Size: 2164 3774
SspI AAT'ATT
Cuts at: 5 52 3862 5
Size: 47 3810 2081
Fragments arranged by size:
3810 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5938
StyI C'CwwG_G
Cuts at: 513 1065 2085 2597 3067 3377 513
Size: 552 1020 512 470 310 3074
Fragments arranged by size:
3074 1020 552 512 470 310
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2098 3053 3965 3982 4135 4320 4765
Size: 1255 518 955 912 17 153 185 445
Cuts at: 4765 325
Size: 1498
Fragments arranged by size:
1498 1255 955 912 518 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2982 4184 5896
Size: 274 80 33 51 1443 1005 1202 1712
Cuts at: 5896 96
Size: 138
Fragments arranged by size:
1712 1443 1202 1005 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2654 2686 5898 5930 2654
Size: 32 3212 32 2662
Fragments arranged by size:
3212 2662 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5938
XbaI T'CTAG_A
Cuts at: 3274 3274
Size: 5938
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2088 2092 3065 3217 2088
Size: 4 973 152 4809
Fragments arranged by size:
4809 973 152 4
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5938
XmaI C'CCGG_G
Cuts at: 1870 2802 3263 1870
Size: 932 461 4545
Fragments arranged by size:
4545 932 461
XmnI GAAnn'nnTTC
Cuts at: 2696 4067 2696
Size: 1371 4567
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3115 3746 276
Size: 53 83 186 936 1581 631 2468
Fragments arranged by size:
2468 1581 936 631 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FseI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NruI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV
FalI FspAI KasI NarI NsiI PacI PmlI PspXI
PsrI RsrII SacI SacII SanDI SexAI SfoI SrfI
SwaI