Restriction map for
pFN21AE0525
(Circular) (Six-base) MAPSORT of: pFN21AE0525.seq Check: 6498 from: 1 to: 5857
pFN21AE0525 5857 bp
With 182 enzymes: SgfI *
October 20, 2009 10:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2061 2861 3040 2061
Size: 800 179 4878
Fragments arranged by size:
4878 800 179
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3667 278
Size: 53 83 186 936 2131 2468
Fragments arranged by size:
2468 2131 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3200 1937
Size: 1263 4594
Acc65I G'GTAC_C
Cuts at: 3178 3178
Size: 5857
AclI AA'CG_TT
Cuts at: 1563 3984 4357 1563
Size: 2421 373 3063
Fragments arranged by size:
3063 2421 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3920 5304 5572 1632
Size: 2288 1384 268 1917
Fragments arranged by size:
2288 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5660
AflIII A'CryG_T
Cuts at: 1203 4756 5733 1203
Size: 3553 977 1327
Fragments arranged by size:
3553 1327 977
AgeI A'CCGG_T
Cuts at: 4688 4688
Size: 5857
AhdI GACnn_n'nnGTC
Cuts at: 4586 4586
Size: 5857
AleI CACnn'nnGTG
Cuts at: 1166 3146 1166
Size: 1980 3877
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2190 1184
Size: 1006 4851
AlwNI CAG_nnn'CTG
Cuts at: 2454 2464 2969 3600 5172 2454
Size: 10 505 631 1572 3139
Fragments arranged by size:
3139 1572 631 505 10
ApaI G_GGCC'C
Cuts at: 2442 2442
Size: 5857
ApaLI G'TGCA_C
Cuts at: 2219 3914 5070 2219
Size: 1695 1156 3006
Fragments arranged by size:
3006 1695 1156
ApoI r'AATT_y
Cuts at: 1493 2414 3166 3392 3544 5653 1493
Size: 921 752 226 152 2109 1697
Fragments arranged by size:
2109 1697 921 752 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4411 160
Size: 872 3379 1606
Fragments arranged by size:
3379 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5857
AvaI C'yCGr_G
Cuts at: 1870 1943 2902 2950 3182 1870
Size: 73 959 48 232 4545
Fragments arranged by size:
4545 959 232 73 48
BamHI G'GATC_C
Cuts at: 3187 3554 3187
Size: 367 5490
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2712 2873 3178 4633 618
Size: 325 217 627 925 161 305 1455 1842
Fragments arranged by size:
1842 1455 925 627 325 305 217 161
BanII G_rGCy'C
Cuts at: 1391 2442 3111 3176 1391
Size: 1051 669 65 4072
Fragments arranged by size:
4072 1051 669 65
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2160 928
Size: 971 261 4625
Fragments arranged by size:
4625 971 261
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4048 4082 1903
Size: 34 2111 34 3678
Fragments arranged by size:
3678 2111 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3749 4959 3749
Size: 1210 4647
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5857
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2270 4468 136
Size: 107 122 71 631 605 598 2198 1525
Fragments arranged by size:
2198 1525 631 605 598 122 107 71
BglII A'GATC_T
Cuts at: 5852 5852
Size: 5857
BlpI GC'TnA_GC
Cuts at: 3024 3274 3024
Size: 250 5607
Bme1580I G_kGCm'C
Cuts at: 1790 2223 2442 2876 3918 5074 1790
Size: 433 219 434 1042 1156 2573
Fragments arranged by size:
2573 1156 1042 434 433 219
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5857
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3588 4546 442
Size: 416 53 83 399 24 2171 958 1753
Fragments arranged by size:
2171 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5857
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2917 2917
Size: 5857
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2051 2131 4517 1239
Size: 812 80 2386 2579
Fragments arranged by size:
2579 2386 812 80
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3982 4847 5145 3982
Size: 865 298 4694
Fragments arranged by size:
4694 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2287 4520 882
Size: 617 788 2233 2219
Fragments arranged by size:
2233 2219 788 617
BsaAI yAC'GTr
Cuts at: 493 1204 2224 3648 493
Size: 711 1020 1424 2702
Fragments arranged by size:
2702 1424 1020 711
BsaBI GATnn'nnATC
Cuts at: 5851 5851
Size: 5857
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3664 4046 4681 275
Size: 53 83 186 936 2131 382 635 1451
Fragments arranged by size:
2131 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2564 4290 4688 4962 5109 5520 2564
Size: 1726 398 274 147 411 2901
Fragments arranged by size:
2901 1726 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5632
Fragments arranged by size:
5632 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2207 1186
Size: 1021 4836
BseYI C'CCAG_C
Cuts at: 5060 5556 5060
Size: 496 5361
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5284
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3307 4068 5096 1540
Size: 275 186 1306 761 1028 2301
Fragments arranged by size:
2301 1306 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2223 2791 2883 3111 3918 4003 5074 2223
Size: 568 92 228 807 85 1071 3006
Fragments arranged by size:
3006 1071 807 568 228 92 85
BsmI GAATG_Cn'
Cuts at: 2042 3368 3461 2042
Size: 1326 93 4438
Fragments arranged by size:
4438 1326 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2197 2197
Size: 5857
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2223 2442 2791 2876 2883 3111 3176
Size: 399 433 219 349 85 7 228 65
Cuts at: 3176 3918 4003 5074 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 433 399 349 228 219
85 85 65 7
BspEI T'CCGG_A
Cuts at: 2564 5520 2564
Size: 2956 2901
BspHI T'CATG_A
Cuts at: 3746 5476 3746
Size: 1730 4127
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2061 2861 3040 3075 3212 844
Size: 1217 800 179 35 137 3489
Fragments arranged by size:
3489 1217 800 179 137 35
BsrBI CCG'CTC
Cuts at: 1394 1823 2059 3744 1394
Size: 429 236 1685 3507
Fragments arranged by size:
3507 1685 429 236
BsrDI GCAATG_nn'
Cuts at: 65 1248 2334 2832 4352 4526 65
Size: 1183 1086 498 1520 174 1396
Fragments arranged by size:
1520 1396 1183 1086 498 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4501 4688 1700
Size: 110 2691 187 2869
Fragments arranged by size:
2869 2691 187 110
BsrGI T'GTAC_A
Cuts at: 96 2736 96
Size: 2640 3217
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5857
BssSI C'ACGA_G
Cuts at: 3917 4929 3917
Size: 1012 4845
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2834 794
Size: 2040 3817
BstBI TT'CG_AA
Cuts at: 2136 4661 2136
Size: 2525 3332
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5857
BstXI CCAn_nnnn'nTGG
Cuts at: 2933 2933
Size: 5857
BstYI r'GATC_y
Cuts at: 1972 3187 3554 3939 3956 5408 5494 5506 5852
Size: 1215 367 385 17 1452 86 12 346
Cuts at: 5852 1972
Size: 1977
Fragments arranged by size:
1977 1452 1215 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2547 513
Size: 552 939 543 3823
Fragments arranged by size:
3823 939 552 543
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5118
Fragments arranged by size:
5118 683 56
BtsI GCAGTG_nn'
Cuts at: 2842 3373 4167 4187 2842
Size: 531 794 20 4512
Fragments arranged by size:
4512 794 531 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5857
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5822
DraI TTT'AAA
Cuts at: 3161 3161
Size: 5857
DraIII CAC_nnn'GTG
Cuts at: 2662 2662
Size: 5857
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4864 809
Size: 507 3548 1802
Fragments arranged by size:
3548 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2775 3304 4193
Size: 54 1425 50 275 18 945 529 889
Cuts at: 4193 5822 8
Size: 1629 43
Fragments arranged by size:
1629 1425 945 889 529 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3304 1537
Size: 275 1492 4090
Fragments arranged by size:
4090 1492 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3787 1353
Size: 2434 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4444 4818 4964 387
Size: 1064 138 2855 374 146 1280
Fragments arranged by size:
2855 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3109 3109
Size: 5857
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2051 2131 3920 4517 5304 5572 1239
Size: 393 419 80 1789 597 787 268 1524
Fragments arranged by size:
1789 1524 787 597 419 393 268 80
EcoRI G'AATT_C
Cuts at: 3166 3544 3166
Size: 378 5479
FspI TGC'GCA
Cuts at: 4363 4363
Size: 5857
HaeII r_GCGC'y
Cuts at: 1678 2016 5004 1678
Size: 338 2988 2531
Fragments arranged by size:
2988 2531 338
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2009 2041 2328 2360 4498
Size: 32 135 32 437 32 287 32 2138
Cuts at: 4498 4530 4572 4604 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2138 437 287 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2341 2628 3201 3447 669
Size: 988 281 403 287 573 246 3079
Fragments arranged by size:
3079 988 573 403 287 281 246
HindIII A'AGCT_T
Cuts at: 748 2111 2130 2399 748
Size: 1363 19 269 4206
Fragments arranged by size:
4206 1363 269 19
HpaI GTT'AAC
Cuts at: 3447 3447
Size: 5857
KpnI G_GTAC'C
Cuts at: 3182 3182
Size: 5857
MfeI C'AATT_G
Cuts at: 3456 3456
Size: 5857
MluI A'CGCG_T
Cuts at: 5733 5733
Size: 5857
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3153 4971 5155 1305
Size: 1848 1818 184 2007
Fragments arranged by size:
2007 1848 1818 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4035
Fragments arranged by size:
4035 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2486 2526 3146 3815 4174 4333 518
Size: 648 1320 40 620 669 359 159 2042
Fragments arranged by size:
2042 1320 669 648 620 359 159 40
MspA1I CmG'CkG
Cuts at: 1606 2189 2200 3273 3950 5098 5343 1606
Size: 583 11 1073 677 1148 245 2120
Fragments arranged by size:
2120 1148 1073 677 583 245 11
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4366
Fragments arranged by size:
4366 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5857
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5857
NotI GC'GGCC_GC
Cuts at: 3304 3304
Size: 5857
NspI r_CATG'y
Cuts at: 1132 1229 3215 4760 1132
Size: 97 1986 1545 2229
Fragments arranged by size:
2229 1986 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2124 1348
Size: 776 5081
PciI A'CATG_T
Cuts at: 4756 4756
Size: 5857
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2482 1108
Size: 1374 4483
PfoI T'CCnGG_A
Cuts at: 2355 2502 2355
Size: 147 5710
PmeI GTTT'AAAC
Cuts at: 3161 3161
Size: 5857
PmlI CAC'GTG
Cuts at: 2224 2224
Size: 5857
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3926 3958 5439 5471 3926
Size: 32 1481 32 4312
Fragments arranged by size:
4312 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5857
PsiI TTA'TAA
Cuts at: 3427 3427
Size: 5857
PspOMI G'GGCC_C
Cuts at: 2438 2438
Size: 5857
PspXI vC'TCGA_Gb
Cuts at: 2950 2950
Size: 5857
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2687 2719 2687
Size: 32 5825
PstI C_TGCA'G
Cuts at: 830 2169 3209 830
Size: 1339 1040 3478
Fragments arranged by size:
3478 1339 1040
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5857
PvuII CAG'CTG
Cuts at: 2200 2200
Size: 5857
SacI G_AGCT'C
Cuts at: 3111 3111
Size: 5857
SalI G'TCGA_C
Cuts at: 1936 3199 1936
Size: 1263 4594
SbfI CC_TGCA'GG
Cuts at: 3209 3209
Size: 5857
ScaI AGT'ACT
Cuts at: 4105 5817 4105
Size: 1712 4145
SfcI C'TryA_G
Cuts at: 826 1045 2165 3133 3205 4340 5021 5212 826
Size: 219 1120 968 72 1135 681 191 1471
Fragments arranged by size:
1471 1135 1120 968 681 219 191 72
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5857
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5857
SmaI CCC'GGG
Cuts at: 1872 3184 1872
Size: 1312 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2950 3961 4862 5124 820
Size: 197 926 1007 1011 901 262 1553
Fragments arranged by size:
1553 1011 1007 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3648 493
Size: 3155 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5857
SphI G_CATG'C
Cuts at: 1132 3215 1132
Size: 2083 3774
SspI AAT'ATT
Cuts at: 5 52 3781 5
Size: 47 3729 2081
Fragments arranged by size:
3729 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5857
StyI C'CwwG_G
Cuts at: 513 1065 2004 2405 3296 513
Size: 552 939 401 891 3074
Fragments arranged by size:
3074 939 891 552 401
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2600 3884 3901 4054 4239 4684 325
Size: 1255 1020 1284 17 153 185 445 1498
Fragments arranged by size:
1498 1284 1255 1020 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2736 4103 5815
Size: 274 80 33 51 1443 759 1367 1712
Cuts at: 5815 96
Size: 138
Fragments arranged by size:
1712 1443 1367 759 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2519 2551 5817 5849 2519
Size: 32 3266 32 2527
Fragments arranged by size:
3266 2527 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5857
XbaI T'CTAG_A
Cuts at: 3193 3193
Size: 5857
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2866 2866
Size: 5857
XhoI C'TCGA_G
Cuts at: 1943 2950 1943
Size: 1007 4850
XmaI C'CCGG_G
Cuts at: 1870 3182 1870
Size: 1312 4545
XmnI GAAnn'nnTTC
Cuts at: 3986 3986
Size: 5857
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3665 276
Size: 53 83 186 936 2131 2468
Fragments arranged by size:
2468 2131 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BstZ17I Bsu36I EcoNI EcoO109I EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PpuMI RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI